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The map label for this gene is acnB [H]

Identifier: 218550943

GI number: 218550943

Start: 3759467

End: 3761989

Strand: Direct

Name: acnB [H]

Synonym: EFER_3669

Alternate gene names: 218550943

Gene position: 3759467-3761989 (Clockwise)

Preceding gene: 218550942

Following gene: 218550944

Centisome position: 81.93

GC content: 52.72

Gene sequence:

>2523_bases
GTGCTGGATGAATACCGCCAACATCAGCGTGCCCGGGCAGCGGCTGGTCTGGTACCAAAGCCCCTGAATGCAGATCAGGT
CCGTGCTCTGACAGATTTGCTGCAAAATCCCCTCTCTGCCGATGACGAAGCATTGTTATATGATCTGCTGGTGAATCGTG
TTCCACCGGGTGTGGATGAAGCCGCTGAAGTCAAAGCTTCATTTCTGGCTGCTATCGCTCATGGCGAACTCGCTTGTGCG
AGAATCAACAAACTACAGGCAATTACATTGCTGGGAACTATGGCTGGAGGCTATAATCTGCCACCCTTGATTGCCGCCCT
TGATGACAACGAACTTGCCTCCACAGCGGTGACAGCTCTCGCCAACACCCTGTTAATCTTCGACTATTTTCAACCAATTG
CCGACAAGGCAACCGCCAAAAACCCCTACGCCATGCAGGTACTACACGCCTGGGCAGCTGGCGACTGGTTTCTTAGCCGT
CCGGCAGTGGCAGAAAAAATCACGCTAACCGTATTCAAAGTAGCCGGAGAAACGAATACCGATGACCTCTCCCCGGCGCA
GGATGTCTGGTCTCGCCCGGATATTCCTCTGCATGCCCTGACGATGCTAAAGAATCCGCGTGAAGGCATTATTGCAGACA
AACCAGGCGAAATTGGTCCGATGCGACAAATCGCCAGTTTGAAAGAGAACGGGTATCCATTAGCTTTCGTTGGCGATACC
GTCGGGACAGGTTCATCACGTAAATCCGCAACAAACTCCTTACTCTGGCTCATGGGAGAAGATATCCCGTGGATCCCGAA
CAAACGCCGGGGCGGCGTGGTGCTGGCAGGGAAAATCGCCCCCATTTTCTTTAATACGCTGCAAGATGCCGGTGCTCTCC
CCATAGAAGCGGATATTTCATCTTTGCAGATGGGCGATATCATCGATATTTACCCAATACGTGGCGAGATACGCGCTCAT
CATAACGACGAACTGCTGGCACGCTTTACGCTCAAAACCGAAGGGATGCTTGATGAAGTACGTGCCGGTGGACGCATTGC
ATTAATTATTGGTCGCGCGCTCACCGCCAAAGCCCGACAAGCGCTGAATCTGCCACCGTCTATGTTATTTCATTCACCGC
AAACAGCTGTAGTAAGTTCCCAGGGATTTACTCAGGCGCAGAAAATAATGGGGCGTGCCTGCGGTGTTGAGGGTGTTCGC
CCCGGCAGCTGGTGTGAACCTAAAATCACATCTATAGGTTCGCCAGATACCAGCGGTCCATTAACACGTGATGAACTCAA
GGACCTGGCTTGCCTGCATTTTTCTGCTGATTTGGTCATGCAGTCCTTCTGTCATACAGCGGCATATCCTGAGCAAATCG
ATGTGGCAACACATCAAACACTGGCAGATTTTATGCGCCAACGAGGTGGTATTGCGCTGCGTCCTGGTGATGGCGTTATC
CACTCCTGGCTTAACCGCATGTTGCTGCCTGATACCGTCGGAACGGGTGGCGATTCGCACACCCGTTTCCCTGTCGGACT
CTCTTTGCCTGCGGGATCTGGGCTGGTGGCATTCGCTGCTGCAACAGGTGTGATGCCACTGGATATGCCAGAATCAGTAC
GGGTCCGTTTTCATGGTGAGCTGCAACCGGGGATCACCTTGCGCGATCTGGTACATGCCATCCCCTGGTATGCCTGCCAG
AACGGCTTACTCACTCTTGAGAAACGTGGCAAGGTTAACGTTTTCTCCGGGCGAATCATTGAAATTGAAGGGCTGGAGCA
TCTCAGTGTCGAGCAGGCCTTTGAGCTGACCGCCGCCACTGCCGAGCGTTCTGCCGCTGGATGCACCATCGCGCTGAGTG
AAGCCTCAGTAATCACCAATATGCAGTCGAATGTTGCCCTGTTGAAAGGGTTGATCGCCGACGGTTATGGTGACCGGAAC
ACTCTGGAGCGTCGCATCAGGAAAATGGAAGCATGGCTGGCAAGCCCAAAATTGCTGGTTGCAGATGCTGATGCGCAATA
TGCCGCTACCCTGGATATTGATCTGAATAGCTTGTGTGAGCCATTACTTTGCGCACCTGACGATCCTGATGATGTACGCC
CACTTTCTGCTCTTAGTGGTGTCTCTATAGATGAGGTGTTTATTGGTTCCTGTATGACCCGTGTTAGTCATTACCGGGCA
ACGGGAGAATTACTTCGTGGGCATAAAGGGCAACTGCCAGCACGCCTGTGGCTGACACCACCAACGCGGATGGATGCCAA
TAAACTACGGGAAGAGGGTTGGTTCAGTGTTTTTGGGCAACGCGGTGGCAGAATTGAGATCCCCGGTTGTTCATTGTGTA
TGGGCAACCAGGCGCGAGTTGCTAATAACGCCACAGTGGTGTCGACATCAACACGTAACTTCCCGAATCGATTAGGTAAT
GGCGCCCATGTTTATCTGGCCTCCGCAGAAGTTGCAGCGATTTGCGCCCGACTGGGACGATTGCCAACGAAGGAGGAGTA
TTTTGCTGCTGTTGCGTCTCTTGTTAGCTGCGAAAGACCTTGA

Upstream 100 bases:

>100_bases
AGTTTACTGACTATCTGCTGGAAGTGTTGGCTGACCTGAACTAACGCACTCCCTCTCCTGGCGGCATTCGTCGCCGGGAT
AAACCAAAAGGAAAATCACC

Downstream 100 bases:

>100_bases
GTGAATACTCCGTTCGTTAAGACTTTATCCTGACGATGCATTTTTCTCGCAAGAAGATAAACTGCCGAATCTGTTTGATA
TTGTATACAATATGGCGCTT

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 840; Mature: 840

Protein sequence:

>840_residues
MLDEYRQHQRARAAAGLVPKPLNADQVRALTDLLQNPLSADDEALLYDLLVNRVPPGVDEAAEVKASFLAAIAHGELACA
RINKLQAITLLGTMAGGYNLPPLIAALDDNELASTAVTALANTLLIFDYFQPIADKATAKNPYAMQVLHAWAAGDWFLSR
PAVAEKITLTVFKVAGETNTDDLSPAQDVWSRPDIPLHALTMLKNPREGIIADKPGEIGPMRQIASLKENGYPLAFVGDT
VGTGSSRKSATNSLLWLMGEDIPWIPNKRRGGVVLAGKIAPIFFNTLQDAGALPIEADISSLQMGDIIDIYPIRGEIRAH
HNDELLARFTLKTEGMLDEVRAGGRIALIIGRALTAKARQALNLPPSMLFHSPQTAVVSSQGFTQAQKIMGRACGVEGVR
PGSWCEPKITSIGSPDTSGPLTRDELKDLACLHFSADLVMQSFCHTAAYPEQIDVATHQTLADFMRQRGGIALRPGDGVI
HSWLNRMLLPDTVGTGGDSHTRFPVGLSLPAGSGLVAFAAATGVMPLDMPESVRVRFHGELQPGITLRDLVHAIPWYACQ
NGLLTLEKRGKVNVFSGRIIEIEGLEHLSVEQAFELTAATAERSAAGCTIALSEASVITNMQSNVALLKGLIADGYGDRN
TLERRIRKMEAWLASPKLLVADADAQYAATLDIDLNSLCEPLLCAPDDPDDVRPLSALSGVSIDEVFIGSCMTRVSHYRA
TGELLRGHKGQLPARLWLTPPTRMDANKLREEGWFSVFGQRGGRIEIPGCSLCMGNQARVANNATVVSTSTRNFPNRLGN
GAHVYLASAEVAAICARLGRLPTKEEYFAAVASLVSCERP

Sequences:

>Translated_840_residues
MLDEYRQHQRARAAAGLVPKPLNADQVRALTDLLQNPLSADDEALLYDLLVNRVPPGVDEAAEVKASFLAAIAHGELACA
RINKLQAITLLGTMAGGYNLPPLIAALDDNELASTAVTALANTLLIFDYFQPIADKATAKNPYAMQVLHAWAAGDWFLSR
PAVAEKITLTVFKVAGETNTDDLSPAQDVWSRPDIPLHALTMLKNPREGIIADKPGEIGPMRQIASLKENGYPLAFVGDT
VGTGSSRKSATNSLLWLMGEDIPWIPNKRRGGVVLAGKIAPIFFNTLQDAGALPIEADISSLQMGDIIDIYPIRGEIRAH
HNDELLARFTLKTEGMLDEVRAGGRIALIIGRALTAKARQALNLPPSMLFHSPQTAVVSSQGFTQAQKIMGRACGVEGVR
PGSWCEPKITSIGSPDTSGPLTRDELKDLACLHFSADLVMQSFCHTAAYPEQIDVATHQTLADFMRQRGGIALRPGDGVI
HSWLNRMLLPDTVGTGGDSHTRFPVGLSLPAGSGLVAFAAATGVMPLDMPESVRVRFHGELQPGITLRDLVHAIPWYACQ
NGLLTLEKRGKVNVFSGRIIEIEGLEHLSVEQAFELTAATAERSAAGCTIALSEASVITNMQSNVALLKGLIADGYGDRN
TLERRIRKMEAWLASPKLLVADADAQYAATLDIDLNSLCEPLLCAPDDPDDVRPLSALSGVSIDEVFIGSCMTRVSHYRA
TGELLRGHKGQLPARLWLTPPTRMDANKLREEGWFSVFGQRGGRIEIPGCSLCMGNQARVANNATVVSTSTRNFPNRLGN
GAHVYLASAEVAAICARLGRLPTKEEYFAAVASLVSCERP
>Mature_840_residues
MLDEYRQHQRARAAAGLVPKPLNADQVRALTDLLQNPLSADDEALLYDLLVNRVPPGVDEAAEVKASFLAAIAHGELACA
RINKLQAITLLGTMAGGYNLPPLIAALDDNELASTAVTALANTLLIFDYFQPIADKATAKNPYAMQVLHAWAAGDWFLSR
PAVAEKITLTVFKVAGETNTDDLSPAQDVWSRPDIPLHALTMLKNPREGIIADKPGEIGPMRQIASLKENGYPLAFVGDT
VGTGSSRKSATNSLLWLMGEDIPWIPNKRRGGVVLAGKIAPIFFNTLQDAGALPIEADISSLQMGDIIDIYPIRGEIRAH
HNDELLARFTLKTEGMLDEVRAGGRIALIIGRALTAKARQALNLPPSMLFHSPQTAVVSSQGFTQAQKIMGRACGVEGVR
PGSWCEPKITSIGSPDTSGPLTRDELKDLACLHFSADLVMQSFCHTAAYPEQIDVATHQTLADFMRQRGGIALRPGDGVI
HSWLNRMLLPDTVGTGGDSHTRFPVGLSLPAGSGLVAFAAATGVMPLDMPESVRVRFHGELQPGITLRDLVHAIPWYACQ
NGLLTLEKRGKVNVFSGRIIEIEGLEHLSVEQAFELTAATAERSAAGCTIALSEASVITNMQSNVALLKGLIADGYGDRN
TLERRIRKMEAWLASPKLLVADADAQYAATLDIDLNSLCEPLLCAPDDPDDVRPLSALSGVSIDEVFIGSCMTRVSHYRA
TGELLRGHKGQLPARLWLTPPTRMDANKLREEGWFSVFGQRGGRIEIPGCSLCMGNQARVANNATVVSTSTRNFPNRLGN
GAHVYLASAEVAAICARLGRLPTKEEYFAAVASLVSCERP

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=834, Percent_Identity=69.3045563549161, Blast_Score=1179, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=361, Percent_Identity=25.4847645429363, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323335, Length=375, Percent_Identity=26.1333333333333, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 90443; Mature: 90443

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDEYRQHQRARAAAGLVPKPLNADQVRALTDLLQNPLSADDEALLYDLLVNRVPPGVDE
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
AAEVKASFLAAIAHGELACARINKLQAITLLGTMAGGYNLPPLIAALDDNELASTAVTAL
HHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEHHHCCCCCCCCEEEEECCCHHHHHHHHHH
ANTLLIFDYFQPIADKATAKNPYAMQVLHAWAAGDWFLSRPAVAEKITLTVFKVAGETNT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCHHHEEEEEEEEEECCCCC
DDLSPAQDVWSRPDIPLHALTMLKNPREGIIADKPGEIGPMRQIASLKENGYPLAFVGDT
CCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
VGTGSSRKSATNSLLWLMGEDIPWIPNKRRGGVVLAGKIAPIFFNTLQDAGALPIEADIS
CCCCCCCCHHCCCEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEECCHH
SLQMGDIIDIYPIRGEIRAHHNDELLARFTLKTEGMLDEVRAGGRIALIIGRALTAKARQ
HCCCCCEEEEEECCCEEECCCCCCEEEEEEECCCCCHHHHCCCCEEEEEECCHHHHHHHH
ALNLPPSMLFHSPQTAVVSSQGFTQAQKIMGRACGVEGVRPGSWCEPKITSIGSPDTSGP
HCCCCHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTRDELKDLACLHFSADLVMQSFCHTAAYPEQIDVATHQTLADFMRQRGGIALRPGDGVI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHH
HSWLNRMLLPDTVGTGGDSHTRFPVGLSLPAGSGLVAFAAATGVMPLDMPESVRVRFHGE
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEHHHCCCCCCCCCCCEEEEEECC
LQPGITLRDLVHAIPWYACQNGLLTLEKRGKVNVFSGRIIEIEGLEHLSVEQAFELTAAT
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCEEEEECCCCCCCHHHHHHHHHHH
AERSAAGCTIALSEASVITNMQSNVALLKGLIADGYGDRNTLERRIRKMEAWLASPKLLV
HHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEE
ADADAQYAATLDIDLNSLCEPLLCAPDDPDDVRPLSALSGVSIDEVFIGSCMTRVSHYRA
EECCCCEEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGELLRGHKGQLPARLWLTPPTRMDANKLREEGWFSVFGQRGGRIEIPGCSLCMGNQARV
HHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCHHHHHCCCCCEEECCCCCEECCCCCEE
ANNATVVSTSTRNFPNRLGNGAHVYLASAEVAAICARLGRLPTKEEYFAAVASLVSCERP
CCCCEEEEECCCCCHHHCCCCCEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDEYRQHQRARAAAGLVPKPLNADQVRALTDLLQNPLSADDEALLYDLLVNRVPPGVDE
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
AAEVKASFLAAIAHGELACARINKLQAITLLGTMAGGYNLPPLIAALDDNELASTAVTAL
HHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEHHHCCCCCCCCEEEEECCCHHHHHHHHHH
ANTLLIFDYFQPIADKATAKNPYAMQVLHAWAAGDWFLSRPAVAEKITLTVFKVAGETNT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCHHHEEEEEEEEEECCCCC
DDLSPAQDVWSRPDIPLHALTMLKNPREGIIADKPGEIGPMRQIASLKENGYPLAFVGDT
CCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
VGTGSSRKSATNSLLWLMGEDIPWIPNKRRGGVVLAGKIAPIFFNTLQDAGALPIEADIS
CCCCCCCCHHCCCEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEECCHH
SLQMGDIIDIYPIRGEIRAHHNDELLARFTLKTEGMLDEVRAGGRIALIIGRALTAKARQ
HCCCCCEEEEEECCCEEECCCCCCEEEEEEECCCCCHHHHCCCCEEEEEECCHHHHHHHH
ALNLPPSMLFHSPQTAVVSSQGFTQAQKIMGRACGVEGVRPGSWCEPKITSIGSPDTSGP
HCCCCHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
LTRDELKDLACLHFSADLVMQSFCHTAAYPEQIDVATHQTLADFMRQRGGIALRPGDGVI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHH
HSWLNRMLLPDTVGTGGDSHTRFPVGLSLPAGSGLVAFAAATGVMPLDMPESVRVRFHGE
HHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEHHHCCCCCCCCCCCEEEEEECC
LQPGITLRDLVHAIPWYACQNGLLTLEKRGKVNVFSGRIIEIEGLEHLSVEQAFELTAAT
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCEEEEECCCCCCCHHHHHHHHHHH
AERSAAGCTIALSEASVITNMQSNVALLKGLIADGYGDRNTLERRIRKMEAWLASPKLLV
HHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEE
ADADAQYAATLDIDLNSLCEPLLCAPDDPDDVRPLSALSGVSIDEVFIGSCMTRVSHYRA
EECCCCEEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGELLRGHKGQLPARLWLTPPTRMDANKLREEGWFSVFGQRGGRIEIPGCSLCMGNQARV
HHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCHHHHHCCCCCEEECCCCCEECCCCCEE
ANNATVVSTSTRNFPNRLGNGAHVYLASAEVAAICARLGRLPTKEEYFAAVASLVSCERP
CCCCEEEEECCCCCHHHCCCCCEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]