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The map label for this gene is aksF [H]

Identifier: 218550942

GI number: 218550942

Start: 3758247

End: 3759410

Strand: Direct

Name: aksF [H]

Synonym: EFER_3668

Alternate gene names: 218550942

Gene position: 3758247-3759410 (Clockwise)

Preceding gene: 218550941

Following gene: 218550943

Centisome position: 81.9

GC content: 52.23

Gene sequence:

>1164_bases
ATGACATTTATCGAAGAGGCTCAGGCGAAATTTGCAGAGTTGATCACCAGCGAACTGGCGCGTATTGCCACCATGAAACA
AGCCGGAGAGGTAAAAGATTTCGCCAGCATGGAGCGGATCGTCGTCGGTATTTTGCCAGGCGATGGTATCGGCCCAATCA
TTATGGCTCAGGCATTACGGGTGCTGGCATTTCTGGCACAAGAGGAGATCGCCGCGGGTAAACTGGAGCTGCGCCCTGTT
AGTGGAATGACCATTGAAGATCGTGCACGTCAGGGGCAAAGCCTTCCTGATGATGTTCTGCAGCAAGTTAAACAGTGTGA
TGTGTTATTAAAAGGCCCGATGGTGACACCGCGTCCGGGTGAAGAATGGCCCAATATGGTCAGTGCGAATAGCCTGCTGC
GCCGTTCACTGGACCTGTTCGCCGCCGTGCGCCCGGTTGCTATTCCTGAGAAGAATATCGACTGGACCTTCTTTCGCGAA
AATATCGAAGGCGAATATATCTGGGGTAACAAAGGGATTCAGGTGACAGAAGATCTGGCGGTGGATTTCAAAGTGCAAAC
CACACCAGGCACTGAGCGCATTGCACGTCAGGCATTTGAGTTTGCGAGAAAAAATGGCAAAACCAACGTCACGGTGATCA
CCAAATCCAATATCGTCAAACTGGCAGATGGTAACTTTCTGCAAGGAGTTCGTGAGGTAGGTAAACGCGAATACCCGGAA
ATTGATGTTCAGGATCGGCTGGTTGATGCCATGTGTGCTCGCATGGCGGACGAAACCTTCTGCAAAGGATTGCAGGTTTT
TGTATTACCAAACCTGTATGGCGATATCGTCACTGATATTGCCGCCGAACTGCAGGGCGGATTGGGTACCGCGGCTTCCG
CCAATATCGGCAACCGCTACGCCATGTTTGAGGCAATTCACGGCACGGCACCGGGTCTTATCAGCGAAGGGCGTGGCGAG
TATGCCGACCCGTGCAGCCTCATTCGTGCGGTAGGCATGTTGCTCGCACATATCGGTTATGGCGATAAGAGCCGTAAGCT
AAGCCAGGCGCTCCACCGTTGTACTGTCAGCGAACGAAAAAAAGTCGTTACCACATGGCCACAGGATGCCAGTGCGGCGG
AGTTTACTGACTATCTGCTGGAAGTGTTGGCTGACCTGAACTAA

Upstream 100 bases:

>100_bases
CGATGACTCCACTTACTCAGGATGAACGTGAAATTATTAAGGCTGGCTGCCTGATTAACTACAACAAAGCTCGTAACCAT
AAGTAAAAAATGGAGTTGTT

Downstream 100 bases:

>100_bases
CGCACTCCCTCTCCTGGCGGCATTCGTCGCCGGGATAAACCAAAAGGAAAATCACCGTGCTGGATGAATACCGCCAACAT
CAGCGTGCCCGGGCAGCGGC

Product: Isocitrate dehydrogenase

Products: NA

Alternate protein names: HICDH; Isohomocitrate dehydrogenase; IHDH; NAD-dependent threo-isohomocitrate dehydrogenase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTFIEEAQAKFAELITSELARIATMKQAGEVKDFASMERIVVGILPGDGIGPIIMAQALRVLAFLAQEEIAAGKLELRPV
SGMTIEDRARQGQSLPDDVLQQVKQCDVLLKGPMVTPRPGEEWPNMVSANSLLRRSLDLFAAVRPVAIPEKNIDWTFFRE
NIEGEYIWGNKGIQVTEDLAVDFKVQTTPGTERIARQAFEFARKNGKTNVTVITKSNIVKLADGNFLQGVREVGKREYPE
IDVQDRLVDAMCARMADETFCKGLQVFVLPNLYGDIVTDIAAELQGGLGTAASANIGNRYAMFEAIHGTAPGLISEGRGE
YADPCSLIRAVGMLLAHIGYGDKSRKLSQALHRCTVSERKKVVTTWPQDASAAEFTDYLLEVLADLN

Sequences:

>Translated_387_residues
MTFIEEAQAKFAELITSELARIATMKQAGEVKDFASMERIVVGILPGDGIGPIIMAQALRVLAFLAQEEIAAGKLELRPV
SGMTIEDRARQGQSLPDDVLQQVKQCDVLLKGPMVTPRPGEEWPNMVSANSLLRRSLDLFAAVRPVAIPEKNIDWTFFRE
NIEGEYIWGNKGIQVTEDLAVDFKVQTTPGTERIARQAFEFARKNGKTNVTVITKSNIVKLADGNFLQGVREVGKREYPE
IDVQDRLVDAMCARMADETFCKGLQVFVLPNLYGDIVTDIAAELQGGLGTAASANIGNRYAMFEAIHGTAPGLISEGRGE
YADPCSLIRAVGMLLAHIGYGDKSRKLSQALHRCTVSERKKVVTTWPQDASAAEFTDYLLEVLADLN
>Mature_386_residues
TFIEEAQAKFAELITSELARIATMKQAGEVKDFASMERIVVGILPGDGIGPIIMAQALRVLAFLAQEEIAAGKLELRPVS
GMTIEDRARQGQSLPDDVLQQVKQCDVLLKGPMVTPRPGEEWPNMVSANSLLRRSLDLFAAVRPVAIPEKNIDWTFFREN
IEGEYIWGNKGIQVTEDLAVDFKVQTTPGTERIARQAFEFARKNGKTNVTVITKSNIVKLADGNFLQGVREVGKREYPEI
DVQDRLVDAMCARMADETFCKGLQVFVLPNLYGDIVTDIAAELQGGLGTAASANIGNRYAMFEAIHGTAPGLISEGRGEY
ADPCSLIRAVGMLLAHIGYGDKSRKLSQALHRCTVSERKKVVTTWPQDASAAEFTDYLLEVLADLN

Specific function: Catalyzes the NAD-dependent oxidation and decarboxylation of (2R,3S)-homoisocitrate ((1R,2S)-1- hydroxybutane-1,2,4-tricarboxylate), (2R,3S)-homo(2)-isocitrate and (2R,3S)-homo(3)-isocitrate, into 2-oxoadipate, 2-oxopimelate, and 2-oxosuberate, respective

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=359, Percent_Identity=33.983286908078, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI4758582, Length=337, Percent_Identity=33.2344213649852, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28178838, Length=337, Percent_Identity=33.2344213649852, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28178816, Length=319, Percent_Identity=32.9153605015674, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28178821, Length=319, Percent_Identity=32.9153605015674, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28178819, Length=168, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=30.08356545961, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787381, Length=403, Percent_Identity=27.5434243176179, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081683, Length=334, Percent_Identity=27.5449101796407, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=32.4561403508772, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25144293, Length=330, Percent_Identity=33.030303030303, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17505779, Length=329, Percent_Identity=31.9148936170213, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17550882, Length=325, Percent_Identity=28.9230769230769, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324709, Length=378, Percent_Identity=32.010582010582, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=356, Percent_Identity=32.3033707865169, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6322097, Length=338, Percent_Identity=31.9526627218935, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319830, Length=326, Percent_Identity=26.6871165644172, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=32.4858757062147, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=32.4858757062147, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24661184, Length=360, Percent_Identity=30.5555555555556, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI161078637, Length=331, Percent_Identity=30.5135951661631, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI161078635, Length=331, Percent_Identity=30.5135951661631, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI161078633, Length=331, Percent_Identity=30.5135951661631, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24650122, Length=331, Percent_Identity=30.5135951661631, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI161078639, Length=326, Percent_Identity=30.3680981595092, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI281362242, Length=331, Percent_Identity=30.8157099697885, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648872, Length=331, Percent_Identity=30.8157099697885, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20130355, Length=315, Percent_Identity=22.8571428571429, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.87 [H]

Molecular weight: Translated: 42476; Mature: 42345

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFIEEAQAKFAELITSELARIATMKQAGEVKDFASMERIVVGILPGDGIGPIIMAQALR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHH
VLAFLAQEEIAAGKLELRPVSGMTIEDRARQGQSLPDDVLQQVKQCDVLLKGPMVTPRPG
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHCCCEEECCCCCCCCC
EEWPNMVSANSLLRRSLDLFAAVRPVAIPEKNIDWTFFRENIEGEYIWGNKGIQVTEDLA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEECCCCCEEECCCE
VDFKVQTTPGTERIARQAFEFARKNGKTNVTVITKSNIVKLADGNFLQGVREVGKREYPE
EEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHHHCCCCCCC
IDVQDRLVDAMCARMADETFCKGLQVFVLPNLYGDIVTDIAAELQGGLGTAASANIGNRY
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
AMFEAIHGTAPGLISEGRGEYADPCSLIRAVGMLLAHIGYGDKSRKLSQALHRCTVSERK
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
KVVTTWPQDASAAEFTDYLLEVLADLN
CEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFIEEAQAKFAELITSELARIATMKQAGEVKDFASMERIVVGILPGDGIGPIIMAQALR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHH
VLAFLAQEEIAAGKLELRPVSGMTIEDRARQGQSLPDDVLQQVKQCDVLLKGPMVTPRPG
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHCCCEEECCCCCCCCC
EEWPNMVSANSLLRRSLDLFAAVRPVAIPEKNIDWTFFRENIEGEYIWGNKGIQVTEDLA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEECCCCCEEECCCE
VDFKVQTTPGTERIARQAFEFARKNGKTNVTVITKSNIVKLADGNFLQGVREVGKREYPE
EEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHHHCCCCCCC
IDVQDRLVDAMCARMADETFCKGLQVFVLPNLYGDIVTDIAAELQGGLGTAASANIGNRY
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
AMFEAIHGTAPGLISEGRGEYADPCSLIRAVGMLLAHIGYGDKSRKLSQALHRCTVSERK
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
KVVTTWPQDASAAEFTDYLLEVLADLN
CEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]