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The map label for this gene is 218550932

Identifier: 218550932

GI number: 218550932

Start: 3745939

End: 3746757

Strand: Direct

Name: 218550932

Synonym: EFER_3658

Alternate gene names: NA

Gene position: 3745939-3746757 (Clockwise)

Preceding gene: 218550930

Following gene: 218550933

Centisome position: 81.63

GC content: 41.51

Gene sequence:

>819_bases
GTGCTGAAAAAGGATAAATTTCATATGCGTTTTGATACCCATGCCCATGTTTTCCGCTTAGACCTACCGTTGGCAGAACA
ATGCCGCTACGTTCCTGAATATGATGCAACACCTGAACATTACCTCTCACATCTTGACCAGCACGGTTTCGATAAAGCGA
TTTTAATCCAGCCAAGTTTTCTGGGAACAGATAACCACTACATGCTTGAGGCTATTCGCCGGTATCCGGATCGTTTTTTC
GGTGTCGCGGTGATCGATATCACAACTTCACTTTCAGCGATGGAAGAGATGAAACGAAACGGCATTGTTGGTATTCGCCT
GAATTTAATTGGACAACCAATCCCCGATCTGACCTCTCCTTTATGGCGTCAATTTCTAATTCAAATTAAGAATCTGGGCT
GGCATGTCGAACTTCATCGTCCGGTAGCAGATTTACGTCCTTTAATTGAAGCATTGTTACAGGCCGGGGTAAAAATCGTT
ATTGACCATTTCGGATTACCCAAAGATAAAGTGCAAGACAGCGGATTTAATTTTCTTCTCAAGCAGGCCAGCTCTCGAAA
TATTTGGGTCAAAATTTCCGCCAGTTATCGTAATGGTGATTATCTATTGCCTGCTGATAATGTTGGGATGCTTCTGTCGC
CCTTGTTGAAGAATGTTGGCGCTGATCGCTTATTATGGGGAAGTGACTGGCCTCATACACGTTATGAAACTCGTATAACA
TATGAAAAAACTGTTCATGAATTTTTTAACTGGCCACTTTCGGAAAAAGAAAGAAACATGATTTTAGAAAAAAGTGTAGT
AGATCTACTAACCCTATAA

Upstream 100 bases:

>100_bases
GTATAGTCAACTACAGTTAAAGTTGACTATACAACTATGTATGATTTGAACGTTTCTACGTGAAAATTGGATTACGTTTT
GCTGATAGTTTATCGCTATC

Downstream 100 bases:

>100_bases
TGGGAAATCATACATAATTCATATAAATAATCAGCATAATTTCTGTCACCCGACTGTGATAAAGATTATTGCGCGCATAT
AACATAATAATATTGAGCAG

Product: hydrolase

Products: NA

Alternate protein names: Amidohydrolase; 2-Pyrone-4 6-Dicarboxylic Acid Hydrolase; Dicarboxylic Acid Hydrolase; Amidohydrolase Family Protein; 2-Pyrone-4 6-Dicarbaxylate Hydrolase; Metal-Dependent Hydrolase; 2-Pyrone-4 6-Dicarboxylate Lactonase; Hydrolase Amidohydrolase 2 Motif; 2-Pyrone-4 6-Dicarboxylate Hydrolase; Metal-Dependent Hydrolase Of TIM-Barrel Fold; Transcriptional Regulator GntR; Aminohydrolase; Metal-Dependent Transmembrane Hydrolase; Metal-Dependent Hydrolase Protein; Metal-Dependent Hydrolase Of TIM-Barrel Fold Family; Amidohydrolase 2 Family; Amidohydrolase Family; Exported Protein

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MLKKDKFHMRFDTHAHVFRLDLPLAEQCRYVPEYDATPEHYLSHLDQHGFDKAILIQPSFLGTDNHYMLEAIRRYPDRFF
GVAVIDITTSLSAMEEMKRNGIVGIRLNLIGQPIPDLTSPLWRQFLIQIKNLGWHVELHRPVADLRPLIEALLQAGVKIV
IDHFGLPKDKVQDSGFNFLLKQASSRNIWVKISASYRNGDYLLPADNVGMLLSPLLKNVGADRLLWGSDWPHTRYETRIT
YEKTVHEFFNWPLSEKERNMILEKSVVDLLTL

Sequences:

>Translated_272_residues
MLKKDKFHMRFDTHAHVFRLDLPLAEQCRYVPEYDATPEHYLSHLDQHGFDKAILIQPSFLGTDNHYMLEAIRRYPDRFF
GVAVIDITTSLSAMEEMKRNGIVGIRLNLIGQPIPDLTSPLWRQFLIQIKNLGWHVELHRPVADLRPLIEALLQAGVKIV
IDHFGLPKDKVQDSGFNFLLKQASSRNIWVKISASYRNGDYLLPADNVGMLLSPLLKNVGADRLLWGSDWPHTRYETRIT
YEKTVHEFFNWPLSEKERNMILEKSVVDLLTL
>Mature_272_residues
MLKKDKFHMRFDTHAHVFRLDLPLAEQCRYVPEYDATPEHYLSHLDQHGFDKAILIQPSFLGTDNHYMLEAIRRYPDRFF
GVAVIDITTSLSAMEEMKRNGIVGIRLNLIGQPIPDLTSPLWRQFLIQIKNLGWHVELHRPVADLRPLIEALLQAGVKIV
IDHFGLPKDKVQDSGFNFLLKQASSRNIWVKISASYRNGDYLLPADNVGMLLSPLLKNVGADRLLWGSDWPHTRYETRIT
YEKTVHEFFNWPLSEKERNMILEKSVVDLLTL

Specific function: Unknown

COG id: COG3618

COG function: function code R; Predicted metal-dependent hydrolase of the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 31557; Mature: 31557

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKDKFHMRFDTHAHVFRLDLPLAEQCRYVPEYDATPEHYLSHLDQHGFDKAILIQPSF
CCCCCCEEEEECCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCC
LGTDNHYMLEAIRRYPDRFFGVAVIDITTSLSAMEEMKRNGIVGIRLNLIGQPIPDLTSP
CCCCCHHHHHHHHHCCHHHHCEEEEEHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHH
LWRQFLIQIKNLGWHVELHRPVADLRPLIEALLQAGVKIVIDHFGLPKDKVQDSGFNFLL
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCHHHHH
KQASSRNIWVKISASYRNGDYLLPADNVGMLLSPLLKNVGADRLLWGSDWPHTRYETRIT
HHCCCCEEEEEEEEEECCCCEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEH
YEKTVHEFFNWPLSEKERNMILEKSVVDLLTL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKKDKFHMRFDTHAHVFRLDLPLAEQCRYVPEYDATPEHYLSHLDQHGFDKAILIQPSF
CCCCCCEEEEECCCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCC
LGTDNHYMLEAIRRYPDRFFGVAVIDITTSLSAMEEMKRNGIVGIRLNLIGQPIPDLTSP
CCCCCHHHHHHHHHCCHHHHCEEEEEHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHH
LWRQFLIQIKNLGWHVELHRPVADLRPLIEALLQAGVKIVIDHFGLPKDKVQDSGFNFLL
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCHHHHH
KQASSRNIWVKISASYRNGDYLLPADNVGMLLSPLLKNVGADRLLWGSDWPHTRYETRIT
HHCCCCEEEEEEEEEECCCCEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEH
YEKTVHEFFNWPLSEKERNMILEKSVVDLLTL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA