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The map label for this gene is yeiN [H]

Identifier: 218549579

GI number: 218549579

Start: 2309962

End: 2310900

Strand: Reverse

Name: yeiN [H]

Synonym: EFER_2251

Alternate gene names: 218549579

Gene position: 2310900-2309962 (Counterclockwise)

Preceding gene: 218549580

Following gene: 218549578

Centisome position: 50.36

GC content: 46.54

Gene sequence:

>939_bases
ATGTCTGAATTAACTCTGTCCCCTGAACTATTACAAATATCTGCCGAAGTACAAGAAGCTTTAAAAAATAAAAAACCGAT
TGTTGCTCTTGAATCAACCATTATTTCGCATGGTATGCCATTTCCGCAAAATGCTCAGACGGCAATTGAAGTAGAAGACA
CAATTCGTAAAAACGGTGCTGTACCTGCAACAATCGCCATTATTGGTGGTGTCATGAAAGTAGGTTTGAGCAAAGAAGAA
ATTGAATTGTTAGGCCGTGAAGGGCATAACGTGACGAAAGTCAGTCGCCGTGATTTACCTTTTGTCGTTGCCGCAGGGAA
AAATGGTGCGACCACCGTTGCTTCAACGATGATTATCGCGGCACTGGCAGGAATTAAAGTATTTGCAACGGGGGGTATTG
GTGGTGTGCATCGCGGTGCGGAACATACATTCGATATTTCTGCCGATTTACAGGAACTGGCAAATACTAATGTCACCGTT
GTTTGTGCCGGTGCAAAATCTATTCTCGATTTAGGATTAACAACAGAATATTTAGAAACCTTCGGTGTACCGTTAATTGG
CTATCAGACAAAAGCGCTGCCTGCGTTTTTCTGCCGTACCAGCCCATTTGAAGTCAGTATTCGCCTTGATAGTGCCACGG
AAATCGCTCGTGCAATGGCAGTGAAATGGCAAACCGGTTTGAACGGCGGCCTGGTAGTGGCGAATCCGATCCCGGAACAG
TTTGCGATGCCGGAGGAATCTATCAATGCAGCCATAGATCAAGCCGTCGCCGAAGCCGAAGAGCAGGGCGTTATTGGTAA
AGAAAGTACACCGTTCCTGCTGGCTCGTGTTGCTGAACTGACTGGCGGTGACAGCCTGAAATCCAACATCCAACTGGTGT
TCAACAACGCCATTCTGGCGAGCGAAATTGCCAAAGAATACCAGCGTCTCGCGGGTTAA

Upstream 100 bases:

>100_bases
GATTTGCGCAGGGATGTTCGTCAATGGCACTTGCCTGTGAATATACCAATAACCCTGAATTATCAATTGCCAATGTTACA
TCGTTAGTGGAGAACTCCGA

Downstream 100 bases:

>100_bases
TTTCTGACGGGCAGAAAGCCGTCGCCCGTACTGATCATACATACCGAAACCTGGCGCTTGCGCCGGGTTTACTGGCATGA
AAGGAAAATCATTATGGATA

Product: hypothetical protein

Products: NA

Alternate protein names: PsiMP glycosidase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSELTLSPELLQISAEVQEALKNKKPIVALESTIISHGMPFPQNAQTAIEVEDTIRKNGAVPATIAIIGGVMKVGLSKEE
IELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARAMAVKWQTGLNGGLVVANPIPEQ
FAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG

Sequences:

>Translated_312_residues
MSELTLSPELLQISAEVQEALKNKKPIVALESTIISHGMPFPQNAQTAIEVEDTIRKNGAVPATIAIIGGVMKVGLSKEE
IELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARAMAVKWQTGLNGGLVVANPIPEQ
FAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG
>Mature_311_residues
SELTLSPELLQISAEVQEALKNKKPIVALESTIISHGMPFPQNAQTAIEVEDTIRKNGAVPATIAIIGGVMKVGLSKEEI
ELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVV
CAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARAMAVKWQTGLNGGLVVANPIPEQF
AMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG

Specific function: Catalyzes the hydrolysis of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. In vitro, can also catalyze the reverse reaction [H]

COG id: COG2313

COG function: function code Q; Uncharacterized enzyme involved in pigment biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine-5'-phosphate glycosidase family [H]

Homologues:

Organism=Escherichia coli, GI1788490, Length=312, Percent_Identity=95.5128205128205, Blast_Score=605, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI25147470, Length=297, Percent_Identity=50.5050505050505, Blast_Score=276, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24580669, Length=295, Percent_Identity=51.5254237288136, Blast_Score=285, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022830
- InterPro:   IPR007342 [H]

Pfam domain/function: PF04227 Indigoidine_A [H]

EC number: NA

Molecular weight: Translated: 32907; Mature: 32776

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTLSPELLQISAEVQEALKNKKPIVALESTIISHGMPFPQNAQTAIEVEDTIRKNGA
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCEEEEEHHHHHHCCC
VPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIA
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHH
ALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLET
HHHCCEEEEECCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCHHHHHHHCCCHHHHHH
FGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARAMAVKWQTGLNGGLVVANPIPEQ
HCCCEECCCHHCCHHHHCCCCCEEEEEEECCHHHHHHHHHHHEECCCCCCEEEECCCCHH
FAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHCCEEEEECCHHHH
SEIAKEYQRLAG
HHHHHHHHHHCC
>Mature Secondary Structure 
SELTLSPELLQISAEVQEALKNKKPIVALESTIISHGMPFPQNAQTAIEVEDTIRKNGA
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCEEEEEHHHHHHCCC
VPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIA
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHH
ALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLET
HHHCCEEEEECCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCHHHHHHHCCCHHHHHH
FGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARAMAVKWQTGLNGGLVVANPIPEQ
HCCCEECCCHHCCHHHHCCCCCEEEEEEECCHHHHHHHHHHHEECCCCCCEEEECCCCHH
FAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHCCEEEEECCHHHH
SEIAKEYQRLAG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]