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The map label for this gene is cpsB [H]

Identifier: 218549464

GI number: 218549464

Start: 2164367

End: 2165803

Strand: Reverse

Name: cpsB [H]

Synonym: EFER_2133

Alternate gene names: 218549464

Gene position: 2165803-2164367 (Counterclockwise)

Preceding gene: 218549465

Following gene: 218549463

Centisome position: 47.2

GC content: 55.53

Gene sequence:

>1437_bases
ATGTCGCAGTCGAAACTCTATCCAGTTGTGATGGCAGGTGGCTCCGGTAGCCGCTTATGGCCGCTTTCCCGCGTACTTTA
TCCCAAGCAGTTTTTATGCCTGAAAGGCGATCTTACCATGCTGCAAACCACCATCTGCCGCCTGAACGGCGTGGAGTGCG
AAAGCCCGGTGGTGATTTGCAATGAGCAGCACCGCTTTATTGTCGCGGAACAGCTGCGTCAACTAAACAAACTCACCGAG
AACATTATTCTCGAACCGGCAGGGCGTAACACTGCACCTGCCATTGCGTTGGCGGCGCTGGCGGCAAAACGTCATAGCCC
GGACAGCGATCCGTTAATGTTGGTCCTGGCGGCAGATCATGTGATTGCCGACGAAGACGCGTTCCGCGCCGCCGTGCGTA
ATGCCATGCCGTATGCCGAAGCGGGCAAGCTGGTCACCTTCGGCATTGTGCCGGATCTACCAGAAACCGGTTATGGCTAT
ATTCGTCGCGGTGAAGTGTCGGCGGGTGAGCAGGATACGGTGGCCTTTGAAGTGGCGCAGTTTGTCGAAAAACCGAATCT
GGAAACCGCTCAGGCCTATGTGGCAAGCGGCGAATATTACTGGAACAGCGGCATGTTCCTGTTCCGCGCCGGACGTTATC
TCGAAGAACTGAAAAAATATCGCCCGGATATTCTCGATGCCTGTGAAAAGGCGATGAGTGCCGTCGATCCGGATCTCGAT
TTTATTCGTGTGGATGAAGAGGCGTTTCTTGCCTGCCCGGAAGAGTCGGTGGATTACGCGGTCATGGAACGTACGGCAGA
TGCCGTTGTGGTGCCGATGGATGCGGGCTGGAGCGATGTCGGTTCCTGGTCCTCTTTATGGGAGATCAGCGCCCACACCG
CGGAAGGCAACGTTTGCCACGGCGATGTGATTAATCACAAAACCGAAAACAGCTACGTGTATGCCGAATCTGGCCTGGTC
ACCACCGTCGGGGTGAAAGATCTGGTAGTGGTGCAGACCAAAGACGCGGTGCTGATTGCAGACCGTAACGCGGTGCAGGA
TGTGAAAAAAGTGGTCGAGCAGATCAAAGCCGATGGTCGCCATGAGCATCGGGTGCATCGCGAAGTGTATCGCCCGTGGG
GCAAATATGACTCTATCGACGCGGGCGACCGCTACCAGGTGAAACGCATCACCGTGAAACCGGGCGAGGGCTTGTCGGTA
CAGATGCACCATCACCGCGCGGAACACTGGGTAGTGGTCGCGGGAACGGCAAAAGTCACCATTGACGGTGATATCAAACT
GCTTGGTGAAAACGAGTCCATTTATATTCCGCTGGGGGCAAAACACTGCCTGGAAAACCCGGGGAAAATTCCCCTCGATT
TAATTGAAGTGCGCTCCGGCTCTTATCTCGAAGAGGATGATGTGGTGCGCTTCGCGGATCGCTACGGACGGGTGTAA

Upstream 100 bases:

>100_bases
TGCTGCCCAAACACAACACGGTGGCACGTGAATATGCCGAACGCACGCTCGATAAAGAGAACGTGTTACGTCAATTTATA
CATGATATTCGGGGATAATT

Downstream 100 bases:

>100_bases
ATGTCGCATCAGGCGCTGTTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTACGGATTTGTAGGCCTGATAAG
ACGCGACAGCGTCGCATCAG

Product: mannose-1-phosphate guanyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTE
NIILEPAGRNTAPAIALAALAAKRHSPDSDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
FIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLV
TTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSV
QMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAKHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV

Sequences:

>Translated_478_residues
MSQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTE
NIILEPAGRNTAPAIALAALAAKRHSPDSDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
FIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLV
TTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSV
QMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAKHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
>Mature_477_residues
SQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTEN
IILEPAGRNTAPAIALAALAAKRHSPDSDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYI
RRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLDF
IRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVT
TVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQ
MHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAKHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV

Specific function: Involved in the biosynthesis of the capsular polysaccharide colanic acid [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=478, Percent_Identity=98.9539748953975, Blast_Score=979, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 53017; Mature: 52886

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVIC
CCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEEE
NEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAAKRHSPDSDPLMLVLAADH
CCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCC
VIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYIRRGEVSAGEQDTVAFEVAQ
EECCCHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
FVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
HHHCCCCCHHHHEEECCCEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
FIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCH
EEEECCHHEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHEEEEEECCCCCEEE
GDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGR
CHHHCCCCCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCC
HEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQMHHHRAEHWVVVAGTAKVT
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEE
IDGDIKLLGENESIYIPLGAKHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
ECCCEEEEECCCEEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVIC
CCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEEE
NEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAAKRHSPDSDPLMLVLAADH
CCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCC
VIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYIRRGEVSAGEQDTVAFEVAQ
EECCCHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
FVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
HHHCCCCCHHHHEEECCCEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
FIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCH
EEEECCHHEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHHEEEEEECCCCCEEE
GDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGR
CHHHCCCCCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCC
HEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQMHHHRAEHWVVVAGTAKVT
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEE
IDGDIKLLGENESIYIPLGAKHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
ECCCEEEEECCCEEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1712067; 11677609 [H]