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The map label for this gene is cpsG [H]

Identifier: 218549463

GI number: 218549463

Start: 2162716

End: 2164086

Strand: Reverse

Name: cpsG [H]

Synonym: EFER_2132

Alternate gene names: 218549463

Gene position: 2164086-2162716 (Counterclockwise)

Preceding gene: 218549464

Following gene: 218549462

Centisome position: 47.16

GC content: 55.0

Gene sequence:

>1371_bases
ATGAAAAAATTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGAACTGAATGAAGATATCGCCTGGCG
TATTGGTCGCGCTTATGGCGAATTTCTCAAACCGAAAACCATTGTTTTAGGCGGTGATGTCCGTCTCACCAGCGAAACCT
TAAAACTGGCGCTGGCAAAAGGGTTACAGGATGCGGGCGTTGACGTGCTGGATATCGGCATGTCCGGCACCGAAGAGATC
TATTTCGCCACGTTCCATCTCGGCGTGGATGGCGGCATCGAAGTTACCGCCAGCCATAATCCGATGGATTACAACGGCAT
GAAACTGGTGCGCGAAGGGGCTCGCCCGATCAGCGGTGATACTGGACTGCGCGACGTCCAGCGTCTGGCAGAAGCCAACG
ATTTTCCTCCCGTCGATGAAACCAAACGCGGTCGCTATCAGCAAATCAACCTGCGCGACGCTTACGTTGATCATCTGTTC
GGTTATATCAACGTCAAAAACCTTACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGTGCGGCGGGTCCGGTGGTGGA
CGCCATCGAAGCCCGCTTTAAAGCCCTCGGCGCACCCGTGGAATTGATCAAAGTACACAACACGCCGGACGGCAATTTCC
CCAACGGTATTCCTAATCCATTGCTGCCGGAATGCCGCGACGATACCCGCAATGCGGTCATTAAACACGGCGCGGATATG
GGTATTGCTTTTGATGGCGATTTTGACCGCTGTTTCCTGTTTGACGAAAAAGGACAGTTTATCGAGGGCTACTACATTGT
CGGCCTGCTGGCAGAAGCGTTCCTCGAAAAAAATCCCGGCGCGAAGATCATCCACGATCCGCGTCTTTCCTGGAACACCG
TTGATGTAGTGACTGCCGCAGGCGGCACCCCGGTGATGTCGAAAACCGGACACGCCTTTATTAAAGAACGTATGCGCAAG
GAAGACGCCATCTATGGTGGCGAAATGAGCGCCCACCATTACTTCCGTGATTTCGCTTACTGCGACAGCGGCATGATCCC
GTGGCTGCTGGTCGCCGAACTGGTGTGCCTGAAAGAAAAAACGCTGGGCGAACTGGTGCGCGACCGCATGGCGGCGTTCC
CGGCAAGCGGTGAGATCAACAGCAAACTGGCGCAACCCGTTGAGGCGATTAACCGCGTCGAACAGCATTTTAGCCGCGAG
GCACTGGCGGTGGATCGCACCGATGGCATCAGCATGACCTTTGCCGACTGGCGCTTTAACCTGCGCTCCTCCAACACCGA
ACCGGTGGTGCGCCTGAATGTGGAATCGCGCGGCGATGTGCCACTGATGGAAGCGCGAACGCGAACTCTGCTGACGTTGC
TGAACGAGTAA

Upstream 100 bases:

>100_bases
CGCATCAGGCAATGAATGCAAAACAGCGATGTAAATAACGACAAAAACAAAATTGGCCGCTTCGGTCAGGGCCCACTATT
GCCTGAAAAAGGGTAACGAT

Downstream 100 bases:

>100_bases
TGTCGGATCTTCCCTTACCCCATTGCGGGTAAGGGGCTAATAACAGGAACAACGATGACAAATCTAAAAAAGCGCGAGCG
AGCGAAAACCAATGCATCGT

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRK
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSRE
ALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE

Sequences:

>Translated_456_residues
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRK
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSRE
ALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE
>Mature_456_residues
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRK
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSRE
ALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=456, Percent_Identity=99.5614035087719, Blast_Score=937, Evalue=0.0,
Organism=Escherichia coli, GI1789566, Length=460, Percent_Identity=26.0869565217391, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 50463; Mature: 50463

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAK
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHH
GLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGD
CHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCC
TGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGYINVKNLTPLKLVINSGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHEEEECCCCEEEEEEECCCC
AAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAA
EEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEC
GGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKEK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TLGELVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSREALAVDRTDGISMTFADWRFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEEEECEEEE
LRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE
ECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSETLKLALAK
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHH
GLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGD
CHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCC
TGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGYINVKNLTPLKLVINSGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHEEEECCCCEEEEEEECCCC
AAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAA
EEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEC
GGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKEK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TLGELVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSREALAVDRTDGISMTFADWRFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEEEECEEEE
LRSSNTEPVVRLNVESRGDVPLMEARTRTLLTLLNE
ECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9673232; 10222209; 11206551; 11258796 [H]