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The map label for this gene is wcaI [H]

Identifier: 218549465

GI number: 218549465

Start: 2165806

End: 2167029

Strand: Reverse

Name: wcaI [H]

Synonym: EFER_2134

Alternate gene names: 218549465

Gene position: 2167029-2165806 (Counterclockwise)

Preceding gene: 218549466

Following gene: 218549464

Centisome position: 47.23

GC content: 55.31

Gene sequence:

>1224_bases
ATGAAAATACTGGTCTACGGCATTAACTACTCGCCGGAGTTAACCGGCATCGGCAAATACACCGGCGAGATGGTGGAATG
GCTGGCGGCACAAGGTCATGAGGTGCGGGTCATTACCGCACCGCCGTACTACCCGCAGTGGCAGGTGGGCGAGAACTATT
CCGCATGGTGGTACAAACGAGAAGAGGGGGCCGCCACGGTGTGGCGCTGCCCGCTGTATGTGCCAAAACAGCCGAGCACC
GTGAAACGCCTGTTACATCTCGGCAGCTTTGCCGCCAGCAGTTTTTTTCCCCTGATGGCGCAACGTCGCTGGAAGCCGGA
TCGCATTATCGGCGTAGTACCAACGCTGTTTTGCACGCCGGGAATGCGCCTGCTGGCGAAACTCTCTGGTGCGCGTACCG
TGCTGCATATTCAGGATTACGAAGTGGATGCCATGCTGGGGCTGGGCCTTGCCGGAAAAGGCAAAGGCGGCAAAGTGGCA
CAGCTGGCGACAGCCTTCGAACGTAGCGGACTGCATAACGTCGATAACGTTTCCACGATTTCGCGCTCGATGATGAATAA
AGCCATCGAAAAAGGCGTGGCGGCGGAAAACGTCATCTTCTTCCCCAATTGGTCGGAAATTGCTCGTTTTCAGCATGTCG
CAGACGCCGATGTTGCTGCCCTTCGTAACCAACTTGGCCTGCCGGATAACAAAAAAATCATTCTTTACTCCGGCAATATT
GGTGAAAAGCAGGGGCTGGAAAACGTTATTGAAGCTGCCGATCGCCTGCGCGATGAACCGCTGATTTTTGCCATTGTCGG
GCAGGGCGGCGGCAAAGCGCGGCTGGAAAAAATGGCGCAGCAGCGCGGACTGCGCAATATGCAATTTTTCCCGCTGCAAT
CGTATGACGCCTTGCCTGCACTGCTGAAGATGGGGGATTGCCATCTGGTGGTGCAAAAACGCGGTGCGGCAGATGCCGTA
TTGCCGTCGAAACTGACCAATATTCTGGCGGTAGGCGGTAACGCGGTAATTACCGCAGAAGCTCACACAGAACTGGGGCA
GCTTTGCGAAACCTTCCCAGGAATTGCGGTTTGCGTAGAACCGGAATCGGTCGAGGCGCTGGTTGCTGGGATTCGTCAGG
CGCTCCTGCTGCCCAAACACAACACGGTGGCACGTGAATATGCCGAACGCACGCTCGATAAAGAGAACGTGTTACGTCAA
TTTATACATGATATTCGGGGATAA

Upstream 100 bases:

>100_bases
ACCGCTGGCTGACGCCGGACGCACTGCTCGCCAGTGACAATGTTCACCCTAACAGCCGCGCCTATTTTCTCGCTGAGAAG
CGTGCCGGAGTACCCGGATT

Downstream 100 bases:

>100_bases
TTATGTCGCAGTCGAAACTCTATCCAGTTGTGATGGCAGGTGGCTCCGGTAGCCGCTTATGGCCGCTTTCCCGCGTACTT
TATCCCAAGCAGTTTTTATG

Product: glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWWYKREEGAATVWRCPLYVPKQPST
VKRLLHLGSFAASSFFPLMAQRRWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADVAALRNQLGLPDNKKIILYSGNI
GEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAV
LPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQ
FIHDIRG

Sequences:

>Translated_407_residues
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWWYKREEGAATVWRCPLYVPKQPST
VKRLLHLGSFAASSFFPLMAQRRWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADVAALRNQLGLPDNKKIILYSGNI
GEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAV
LPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQ
FIHDIRG
>Mature_407_residues
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWWYKREEGAATVWRCPLYVPKQPST
VKRLLHLGSFAASSFFPLMAQRRWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADVAALRNQLGLPDNKKIILYSGNI
GEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAV
LPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQ
FIHDIRG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=407, Percent_Identity=98.034398034398, Blast_Score=821, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 44827; Mature: 44827

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWWYKR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
EEGAATVWRCPLYVPKQPSTVKRLLHLGSFAASSFFPLMAQRRWKPDRIIGVVPTLFCTP
CCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCC
GMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVAQLATAFERSGLHNVDNVSTI
HHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
SRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADVAALRNQLGLPDNKKIILYSGNI
HHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCC
GEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPA
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHH
LLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVE
HHHCCCEEEEEECCCCCCHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHHCCCCEEEEC
PESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQFIHDIRG
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWWYKR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
EEGAATVWRCPLYVPKQPSTVKRLLHLGSFAASSFFPLMAQRRWKPDRIIGVVPTLFCTP
CCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCC
GMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVAQLATAFERSGLHNVDNVSTI
HHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
SRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADVAALRNQLGLPDNKKIILYSGNI
HHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEECCC
GEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPA
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHH
LLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVE
HHHCCCEEEEEECCCCCCHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHHCCCCEEEEC
PESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQFIHDIRG
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]