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The map label for this gene is queF

Identifier: 218547687

GI number: 218547687

Start: 282182

End: 283030

Strand: Reverse

Name: queF

Synonym: EFER_0266

Alternate gene names: 218547687

Gene position: 283030-282182 (Counterclockwise)

Preceding gene: 218547692

Following gene: 218547686

Centisome position: 6.17

GC content: 49.12

Gene sequence:

>849_bases
ATGTCTTCTTATGAAAACCATCAGGCGCTTCACAGCCTGACTCTCGGGAAATCAACTGATTACCGGGATACTTATGACGC
CAGCCTGTTGCAGGGTGTGCCACGCAGTCTGAATCGCGATCCGCTGGGATTGACCGCAGATAATCTGCCTTTTCATGGCG
CAGATATCTGGACGTTATATGAATTGTCATGGCTGAATGCGAAAGGTCTGCCACAAGTTGCGGTCGGGCATGTAGAACTG
GATTACACTAGCGTCAATCTTATTGAGTCGAAAAGCTTTAAGTTGTATCTCAACAGCTTTAACCAGACGCGCTTTGACAG
TTGGGAGGATGTTCGTCAGACGCTGGAGCGTGATCTACGTGCCTGCGCCCAGGGGGAAGTCAGTGTTGCCCTCTATCAAC
TTGATGAACTGGAAGGGCAACCCATTGCTCACCTGCATGGCACCTGCATTGATAATCAGGATATCGAAATTGATCACTAT
GATTTCAATGCTGATTATCTGCAAAATGCCACCAGCGGTGAAAAAGTGGTCGAAGAGACGCTGGTCAGCCATCTGCTGAA
ATCAAACTGCCTTATAACCCACCAGCCTGACTGGGGATCTATTCAAATTCAGTATCGTGGTCGTAAGATTGATCGCGAGA
AACTGCTGCGTTATCTGGTCTCTTTCCGCCACCATAACGAGTTCCATGAACAGTGCGTTGAGCGTATTTTCAGCGATATA
TTGCGCTTATGCCAGCCAGAAGAACTTAGCGTCTATGCCCGTTATACGAGGCGCGGCGGTCTGGATATTAACCCATGGCG
CAGCAACTGCAATTTCCAGCCCTCTACCGGGCGTCTGGTTCGTCAGTAA

Upstream 100 bases:

>100_bases
TCTCGTAGGGCTTGCGCGGTCAATTCGTCCACTAATTTCTCTCTTCACATAAGCCAGGATACACTTGTCGCTTAGTGTAA
CTGGTTTATCACGGTGAAAC

Downstream 100 bases:

>100_bases
ACTTTATTTATCATTTTTGCGTGGCAGATTCCGCGCGCAAGGTTGTTAAATGTCATAAGGCAGGGCTATTGTAATCAGCA
GGGAATGACGACACTCGTCC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSSYENHQALHSLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLYELSWLNAKGLPQVAVGHVEL
DYTSVNLIESKSFKLYLNSFNQTRFDSWEDVRQTLERDLRACAQGEVSVALYQLDELEGQPIAHLHGTCIDNQDIEIDHY
DFNADYLQNATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFSDI
LRLCQPEELSVYARYTRRGGLDINPWRSNCNFQPSTGRLVRQ

Sequences:

>Translated_282_residues
MSSYENHQALHSLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLYELSWLNAKGLPQVAVGHVEL
DYTSVNLIESKSFKLYLNSFNQTRFDSWEDVRQTLERDLRACAQGEVSVALYQLDELEGQPIAHLHGTCIDNQDIEIDHY
DFNADYLQNATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFSDI
LRLCQPEELSVYARYTRRGGLDINPWRSNCNFQPSTGRLVRQ
>Mature_281_residues
SSYENHQALHSLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLYELSWLNAKGLPQVAVGHVELD
YTSVNLIESKSFKLYLNSFNQTRFDSWEDVRQTLERDLRACAQGEVSVALYQLDELEGQPIAHLHGTCIDNQDIEIDHYD
FNADYLQNATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFSDIL
RLCQPEELSVYARYTRRGGLDINPWRSNCNFQPSTGRLVRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=282, Percent_Identity=87.5886524822695, Blast_Score=524, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_ESCF3 (B7LVW9)

Other databases:

- EMBL:   CU928158
- RefSeq:   YP_002381478.1
- ProteinModelPortal:   B7LVW9
- EnsemblBacteria:   EBESCT00000124112
- GeneID:   7121501
- GenomeReviews:   CU928158_GR
- KEGG:   efe:EFER_0266
- GeneTree:   EBGT00050000011597
- HOGENOM:   HBG289139
- ProtClustDB:   PRK11792
- BioCyc:   EFER585054:EFER_0266-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 32602; Mature: 32471

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYENHQALHSLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLY
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQTRFDSWEDVRQTLERDLR
EEEECCCCCCCCEEEEEEEEEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
ACAQGEVSVALYQLDELEGQPIAHLHGTCIDNQDIEIDHYDFNADYLQNATSGEKVVEET
HHHCCCCEEEEEEHHHCCCCCCHHCCCEEECCCCCEEEEEECCHHHHHCCCCHHHHHHHH
LVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFSDI
HHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LRLCQPEELSVYARYTRRGGLDINPWRSNCNFQPSTGRLVRQ
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SSYENHQALHSLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLTADNLPFHGADIWTLY
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQTRFDSWEDVRQTLERDLR
EEEECCCCCCCCEEEEEEEEEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
ACAQGEVSVALYQLDELEGQPIAHLHGTCIDNQDIEIDHYDFNADYLQNATSGEKVVEET
HHHCCCCEEEEEEHHHCCCCCCHHCCCEEECCCCCEEEEEECCHHHHHCCCCHHHHHHHH
LVSHLLKSNCLITHQPDWGSIQIQYRGRKIDREKLLRYLVSFRHHNEFHEQCVERIFSDI
HHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LRLCQPEELSVYARYTRRGGLDINPWRSNCNFQPSTGRLVRQ
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA