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The map label for this gene is citE [H]

Identifier: 218547473

GI number: 218547473

Start: 33527

End: 34471

Strand: Direct

Name: citE [H]

Synonym: EFER_0034

Alternate gene names: 218547473

Gene position: 33527-34471 (Clockwise)

Preceding gene: 218547472

Following gene: 218547474

Centisome position: 0.73

GC content: 52.17

Gene sequence:

>945_bases
GTGTGTGTTACGTGCTCGCGTACAGGCAGCGGTAATGCGTGCTGCAGAACAAACGGAAATTCAGTGGGGGGCAATATGAA
ACCACGTCGCAGTATGTTGTTTATCCCGGGTGCAAATGCCGCCATGTTAAGTACTTCCTTCGTTTATGGCGCAGATGCCG
TCATGTTCGATCTGGAAGATGCTGTTTCTCTGCGTGAAAAAGATTCCGCTCGTCTGCTGGTACATCACGCACTACAGCAT
CCGCTGTATCAGGATGTAGAAACCGTTGTCCGTATCAACCCACTCAATACGCCGTTTGGTCTGGCTGATCTGGAAGCTGT
AGTTCGTGCCGGAGTGAATATTGTTCGCCTGCCGAAAACTGACAGTAAAGAAGATGTTCTGGAGCTGGAAGCGCACGTTG
AGCGTATTGAACGTGAATGCGGACGTGAGCCTGGCAGCACGAAATTAATGGCGGCAATTGAGTCTGCTGTGGGTGTTGTC
AATGCGGTTGAGATCGCGACATCTTCCCCGCGCATGGTCGCTATTGCTCTGGCGGCTTTTGACTATGTGATGGATATGGG
CACCAGCCGTGGCGATGGCACTGAGCTATTTTACGCGCGTTGTGCGGTATTACATGCAGCTCGTGTGGCGAAAATTGCGG
CCTACGATGTGGTGTGGTCAGACATTAACGATGAAGCGGGATTTGTCAAAGAAGCGCAACTGGCGAAAAACCTTGGTTTT
AACGGTAAATCCCTGGTAAACCCTCGTCAGATCGAGATGCTGCACCAGGTTTATGCTCCGACGCGTGCTGAAGTAGGTCA
TGCACTGGAGGTGATTGCTGCGGCAGAAGACGCCGAAGAACGCGGCCTGGGCGTGGTGTCATTAAACGGCAAGATGATAG
ATGGCCCGATTATCGATCACGCACGTAAAGTGGTGGCGCTGTCGGCTTCCGGATTTCGCGACTAA

Upstream 100 bases:

>100_bases
AACAATTTGGCAACGCCATTGAACGCGTTGCCCGCGAAACCCTGGCGAAGCTGGGCGTGGAACGTGCACACGTAGTGATT
GATGACAAAGGTGCGCTGGA

Downstream 100 bases:

>100_bases
GGACAGGGAACAATGAAAGACACCGTATCGATGCTGAATCAGCAATATGTTATGCCGGAAGGATTACAACCTTACGCGGG
TGTGACTGCAAAAAGCCCAT

Product: citrate lyase, citryl-ACP lyase subunit beta

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MCVTCSRTGSGNACCRTNGNSVGGNMKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVSLREKDSARLLVHHALQH
PLYQDVETVVRINPLNTPFGLADLEAVVRAGVNIVRLPKTDSKEDVLELEAHVERIERECGREPGSTKLMAAIESAVGVV
NAVEIATSSPRMVAIALAAFDYVMDMGTSRGDGTELFYARCAVLHAARVAKIAAYDVVWSDINDEAGFVKEAQLAKNLGF
NGKSLVNPRQIEMLHQVYAPTRAEVGHALEVIAAAEDAEERGLGVVSLNGKMIDGPIIDHARKVVALSASGFRD

Sequences:

>Translated_314_residues
MCVTCSRTGSGNACCRTNGNSVGGNMKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVSLREKDSARLLVHHALQH
PLYQDVETVVRINPLNTPFGLADLEAVVRAGVNIVRLPKTDSKEDVLELEAHVERIERECGREPGSTKLMAAIESAVGVV
NAVEIATSSPRMVAIALAAFDYVMDMGTSRGDGTELFYARCAVLHAARVAKIAAYDVVWSDINDEAGFVKEAQLAKNLGF
NGKSLVNPRQIEMLHQVYAPTRAEVGHALEVIAAAEDAEERGLGVVSLNGKMIDGPIIDHARKVVALSASGFRD
>Mature_314_residues
MCVTCSRTGSGNACCRTNGNSVGGNMKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVSLREKDSARLLVHHALQH
PLYQDVETVVRINPLNTPFGLADLEAVVRAGVNIVRLPKTDSKEDVLELEAHVERIERECGREPGSTKLMAAIESAVGVV
NAVEIATSSPRMVAIALAAFDYVMDMGTSRGDGTELFYARCAVLHAARVAKIAAYDVVWSDINDEAGFVKEAQLAKNLGF
NGKSLVNPRQIEMLHQVYAPTRAEVGHALEVIAAAEDAEERGLGVVSLNGKMIDGPIIDHARKVVALSASGFRD

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=296, Percent_Identity=26.0135135135135, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI87081764, Length=290, Percent_Identity=61.7241379310345, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=28.0936454849498, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 33695; Mature: 33695

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVTCSRTGSGNACCRTNGNSVGGNMKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLED
CEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEHHH
AVSLREKDSARLLVHHALQHPLYQDVETVVRINPLNTPFGLADLEAVVRAGVNIVRLPKT
HHHHHCCCCHHHHHHHHHHCHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCEEECCCC
DSKEDVLELEAHVERIERECGREPGSTKLMAAIESAVGVVNAVEIATSSPRMVAIALAAF
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHH
DYVMDMGTSRGDGTELFYARCAVLHAARVAKIAAYDVVWSDINDEAGFVKEAQLAKNLGF
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
NGKSLVNPRQIEMLHQVYAPTRAEVGHALEVIAAAEDAEERGLGVVSLNGKMIDGPIIDH
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCEEEEECCEEECCHHHHH
ARKVVALSASGFRD
HHHEEEEECCCCCC
>Mature Secondary Structure
MCVTCSRTGSGNACCRTNGNSVGGNMKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLED
CEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEEEEHHH
AVSLREKDSARLLVHHALQHPLYQDVETVVRINPLNTPFGLADLEAVVRAGVNIVRLPKT
HHHHHCCCCHHHHHHHHHHCHHHHHHHHHEEECCCCCCCCHHHHHHHHHCCCCEEECCCC
DSKEDVLELEAHVERIERECGREPGSTKLMAAIESAVGVVNAVEIATSSPRMVAIALAAF
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHH
DYVMDMGTSRGDGTELFYARCAVLHAARVAKIAAYDVVWSDINDEAGFVKEAQLAKNLGF
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
NGKSLVNPRQIEMLHQVYAPTRAEVGHALEVIAAAEDAEERGLGVVSLNGKMIDGPIIDH
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCEEEEECCEEECCHHHHH
ARKVVALSASGFRD
HHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2205500; 7830578 [H]