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The map label for this gene is citF [H]

Identifier: 218547474

GI number: 218547474

Start: 34484

End: 36004

Strand: Direct

Name: citF [H]

Synonym: EFER_0035

Alternate gene names: 218547474

Gene position: 34484-36004 (Clockwise)

Preceding gene: 218547473

Following gene: 218547475

Centisome position: 0.75

GC content: 50.95

Gene sequence:

>1521_bases
ATGAAAGACACCGTATCGATGCTGAATCAGCAATATGTTATGCCGGAAGGATTACAACCTTACGCGGGTGTGACTGCAAA
AAGCCCATGGCTGGCGGATGAAAAAGAAAAACGTAGCCGTAAAATTTGTGACTCTCTGGAAGATGCCATTCGCCGCTCAG
GTCTGCAAAACGGCATGACCATCTCCTTTCACCATGCGTTTCGTGGTGGCGATAAAGTTGTTAACCTGGTGGTAGCGAAA
CTGGCTGAAATGGGCTTTCGCGATCTGACACTGGCCTCCAGTTCACTGATTGATGCCCACTGGCCGCTGATTGAGCACAT
TAAAAATGGCGTCATTCGCCAGATCTACACTTCCGGCCTGCGCGGCAAACTGGGTGAAGAAATCTCTGCCGGGCTGATGG
AAAATCCGGTACAAATTCACTCCCACGGTGGTCGTGCGCACCTGGTGAAATCGGGTGAGCTGAGCATTGACGTTGCATTT
CTCGGTGTACCGTGCTGTGACGAATTTGGTAACGCCAACGGATTTAGCGGTAAATCTCGTTGCGGTTCACTGGGCTATGC
CAAAGTGGATGCCGATGCGGCGAAATGTGTGGTACTGCTGACAGAAGAGTGGGTCGATTATCCTAACTACCCAGCGAGTA
TTGCTCAGGATCAGGTTGATCTCATTGTCCAGGTCGAAGAAGTCGGTGATCCGGCAAAGATCACTGCAGGTGCTATTCGC
CTGACCAGCAATCCTCGCGAACTGTTAATAGCCCGCAAGGCAGCGAATGTTATTGAGCATTCTGGTTATTTCAAAGACGG
ATTCTCGCTACAAACCGGTACTGGCGGTGCCTCGTTGGCAGTGACGCGTTTCCTCGAAGATAAAATGCGCCGTCTTAATA
TTACCGCTGGTTTTGGCCTTGGCGGGATCACTGGCACGATGGTTGATCTGCATGAAAAAGGGCTGATCAAAGCGCTGCTT
GATACCCAATCATTCGATGGAGATGCCGCTCGCTCGCTGGCAACTAACCCGAATCATATCGAAATTTCAGCGAATCAATA
TGCTAACCCCAGCTCTAAAGGTGTCTCTTGTGAGCGACTGAATGTGGTGATGCTGAGTGCGCTGGAAATTGACGTTAACT
TCAACGTTAACGTCATGACTGGTTCCAATGGCGTACTGCGCGGTGCATCCGGTGGTCATAGTGACACCGCAGCTGGTGCT
GATCTCACTATTATCACCGCACCCTTAGTGCGTGGTCGTATTCCGTGTGTCGTGGAAAAAGTACTGACCACCATAACACC
GGGCGCCAGTGTTGACGTGCTGGTTACCGATCATGGTATTGCTGTTAACCCGGCACGTCAGGATCTGATCGACAATCTGC
GTGAAGCGGGTGTGCCGTTAATGACCATTGAACAGCTCCAGGCACGTGCCGAAATGCTCACCGGCAAACCAGAACCCATC
GAATTTACTGATCGGGTGGTAGCTGTGGTGCGTTATCGTGATGGATCGGTCATTGATGTGATTCGCCAGGTGAAAAACTG
A

Upstream 100 bases:

>100_bases
GTCATTAAACGGCAAGATGATAGATGGCCCGATTATCGATCACGCACGTAAAGTGGTGGCGCTGTCGGCTTCCGGATTTC
GCGACTAAGGACAGGGAACA

Downstream 100 bases:

>100_bases
TGAATGGCGCAGACTTGCTGTCAGGCGGTCAGCCTGTCACGCTCGAAGAAATGCTGCTGGCTCGCGAAAAGCGGGCAGCA
AGGCAACGGCATGCAGTGGC

Product: citrate lyase, citrate-ACP transferase alpha subunit

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKDTVSMLNQQYVMPEGLQPYAGVTAKSPWLADEKEKRSRKICDSLEDAIRRSGLQNGMTISFHHAFRGGDKVVNLVVAK
LAEMGFRDLTLASSSLIDAHWPLIEHIKNGVIRQIYTSGLRGKLGEEISAGLMENPVQIHSHGGRAHLVKSGELSIDVAF
LGVPCCDEFGNANGFSGKSRCGSLGYAKVDADAAKCVVLLTEEWVDYPNYPASIAQDQVDLIVQVEEVGDPAKITAGAIR
LTSNPRELLIARKAANVIEHSGYFKDGFSLQTGTGGASLAVTRFLEDKMRRLNITAGFGLGGITGTMVDLHEKGLIKALL
DTQSFDGDAARSLATNPNHIEISANQYANPSSKGVSCERLNVVMLSALEIDVNFNVNVMTGSNGVLRGASGGHSDTAAGA
DLTIITAPLVRGRIPCVVEKVLTTITPGASVDVLVTDHGIAVNPARQDLIDNLREAGVPLMTIEQLQARAEMLTGKPEPI
EFTDRVVAVVRYRDGSVIDVIRQVKN

Sequences:

>Translated_506_residues
MKDTVSMLNQQYVMPEGLQPYAGVTAKSPWLADEKEKRSRKICDSLEDAIRRSGLQNGMTISFHHAFRGGDKVVNLVVAK
LAEMGFRDLTLASSSLIDAHWPLIEHIKNGVIRQIYTSGLRGKLGEEISAGLMENPVQIHSHGGRAHLVKSGELSIDVAF
LGVPCCDEFGNANGFSGKSRCGSLGYAKVDADAAKCVVLLTEEWVDYPNYPASIAQDQVDLIVQVEEVGDPAKITAGAIR
LTSNPRELLIARKAANVIEHSGYFKDGFSLQTGTGGASLAVTRFLEDKMRRLNITAGFGLGGITGTMVDLHEKGLIKALL
DTQSFDGDAARSLATNPNHIEISANQYANPSSKGVSCERLNVVMLSALEIDVNFNVNVMTGSNGVLRGASGGHSDTAAGA
DLTIITAPLVRGRIPCVVEKVLTTITPGASVDVLVTDHGIAVNPARQDLIDNLREAGVPLMTIEQLQARAEMLTGKPEPI
EFTDRVVAVVRYRDGSVIDVIRQVKN
>Mature_506_residues
MKDTVSMLNQQYVMPEGLQPYAGVTAKSPWLADEKEKRSRKICDSLEDAIRRSGLQNGMTISFHHAFRGGDKVVNLVVAK
LAEMGFRDLTLASSSLIDAHWPLIEHIKNGVIRQIYTSGLRGKLGEEISAGLMENPVQIHSHGGRAHLVKSGELSIDVAF
LGVPCCDEFGNANGFSGKSRCGSLGYAKVDADAAKCVVLLTEEWVDYPNYPASIAQDQVDLIVQVEEVGDPAKITAGAIR
LTSNPRELLIARKAANVIEHSGYFKDGFSLQTGTGGASLAVTRFLEDKMRRLNITAGFGLGGITGTMVDLHEKGLIKALL
DTQSFDGDAARSLATNPNHIEISANQYANPSSKGVSCERLNVVMLSALEIDVNFNVNVMTGSNGVLRGASGGHSDTAAGA
DLTIITAPLVRGRIPCVVEKVLTTITPGASVDVLVTDHGIAVNPARQDLIDNLREAGVPLMTIEQLQARAEMLTGKPEPI
EFTDRVVAVVRYRDGSVIDVIRQVKN

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=479, Percent_Identity=72.8601252609603, Blast_Score=709, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 54308; Mature: 54308

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTVSMLNQQYVMPEGLQPYAGVTAKSPWLADEKEKRSRKICDSLEDAIRRSGLQNGMT
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
ISFHHAFRGGDKVVNLVVAKLAEMGFRDLTLASSSLIDAHWPLIEHIKNGVIRQIYTSGL
EEEEECCCCCHHHHHHHHHHHHHCCCHHEECCCCCHHHHCCHHHHHHHHHHHHHHHHHCC
RGKLGEEISAGLMENPVQIHSHGGRAHLVKSGELSIDVAFLGVPCCDEFGNANGFSGKSR
CCCCCHHHHHHHHCCCEEEECCCCEEEEEECCCEEEEEEEECCCCHHHCCCCCCCCCCCC
CGSLGYAKVDADAAKCVVLLTEEWVDYPNYPASIAQDQVDLIVQVEEVGDPAKITAGAIR
CCCCCEEEECCCCCEEEEEEEHHHCCCCCCCHHHHCCCEEEEEEEECCCCCCEEEEEEEE
LTSNPRELLIARKAANVIEHSGYFKDGFSLQTGTGGASLAVTRFLEDKMRRLNITAGFGL
ECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHEEEEECCCC
GGITGTMVDLHEKGLIKALLDTQSFDGDAARSLATNPNHIEISANQYANPSSKGVSCERL
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHE
NVVMLSALEIDVNFNVNVMTGSNGVLRGASGGHSDTAAGADLTIITAPLVRGRIPCVVEK
EEEEEEEEEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEECHHHCCCCHHHHHH
VLTTITPGASVDVLVTDHGIAVNPARQDLIDNLREAGVPLMTIEQLQARAEMLTGKPEPI
HHHHCCCCCCEEEEEECCCEEECCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCC
EFTDRVVAVVRYRDGSVIDVIRQVKN
CHHCEEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MKDTVSMLNQQYVMPEGLQPYAGVTAKSPWLADEKEKRSRKICDSLEDAIRRSGLQNGMT
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
ISFHHAFRGGDKVVNLVVAKLAEMGFRDLTLASSSLIDAHWPLIEHIKNGVIRQIYTSGL
EEEEECCCCCHHHHHHHHHHHHHCCCHHEECCCCCHHHHCCHHHHHHHHHHHHHHHHHCC
RGKLGEEISAGLMENPVQIHSHGGRAHLVKSGELSIDVAFLGVPCCDEFGNANGFSGKSR
CCCCCHHHHHHHHCCCEEEECCCCEEEEEECCCEEEEEEEECCCCHHHCCCCCCCCCCCC
CGSLGYAKVDADAAKCVVLLTEEWVDYPNYPASIAQDQVDLIVQVEEVGDPAKITAGAIR
CCCCCEEEECCCCCEEEEEEEHHHCCCCCCCHHHHCCCEEEEEEEECCCCCCEEEEEEEE
LTSNPRELLIARKAANVIEHSGYFKDGFSLQTGTGGASLAVTRFLEDKMRRLNITAGFGL
ECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHEEEEECCCC
GGITGTMVDLHEKGLIKALLDTQSFDGDAARSLATNPNHIEISANQYANPSSKGVSCERL
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHE
NVVMLSALEIDVNFNVNVMTGSNGVLRGASGGHSDTAAGADLTIITAPLVRGRIPCVVEK
EEEEEEEEEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEECHHHCCCCHHHHHH
VLTTITPGASVDVLVTDHGIAVNPARQDLIDNLREAGVPLMTIEQLQARAEMLTGKPEPI
HHHHCCCCCCEEEEEECCCEEECCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCC
EFTDRVVAVVRYRDGSVIDVIRQVKN
CHHCEEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7830578; 773936 [H]