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The map label for this gene is appA [H]

Identifier: 218235957

GI number: 218235957

Start: 215815

End: 217542

Strand: Direct

Name: appA [H]

Synonym: BCB4264_A0245

Alternate gene names: 218235957

Gene position: 215815-217542 (Clockwise)

Preceding gene: 218231435

Following gene: 218235203

Centisome position: 3.98

GC content: 34.49

Gene sequence:

>1728_bases
ATGAAGAAAAAGACAAAAAAATGGGCTGGTGTATTTTCGGTATTAATGAGTAGTTCGCTTGTGTTGTCTGCTTGTGGGGG
ACAGGAGGACGCGGCTTCTACAGAGCCGGTTAAACAGCAGGATTTAAAAAATGTGAAAATTGAAAAAATCGCTGCGACAG
ATAAAACGAAAGTGCCAGACAAAGCGAAGAATAGAAAAGATACGTTAGTAGTTGGTATTAGTAAGCCAGGCGGCGTATTT
TTGCCATACTTCCAACAAAATGGTTGGGATGGAAACGTAACTTCAGTTATTTTTGCATCTCTCGTATCAACCGATAAACA
AGGGAAACCAATTCCAGAATTAGCTGAGAAGTGGGACGTTTCTTCTGACCAGTTAACATATACATTCCATTTACGTAAAG
ATTTGAAATTTAGTGATGGATCGCCATTAACGGCGGATGATGTAGCTTTCACATTGACGCTTTTACATGATAAGGCATAT
GAAGGTGAAGTAGATATCTCTCAATATGCGGTTAAAGGTGGTAAAGAATATAAAGAAGGGAAAGCAACTTCAATTGAAGG
TATTCAAGTTGTTGATCCACAAACAATTAAAATTACAACTGAAAAAGTGAATTCACAAGCTATATTTGTTTTAGGAGGTA
CAGTACTATCAAAAGCTTATTATGGAAAAGATTATAAGCAAAATACAAGCTTAGATTACTTAAAAGATTTATATGGAAAG
CCATTAGCTGCAGGTCCATATAAATTTGAAAAGTATATTCCAGGTCAAGAAGTTCGTTTTGTAGCAAACGAAAATTATTA
TGCGGGTAAACCAAAAATTCAAAACTTCATTTATAAAATTACTTCTGGTGATACGAAGCTTCAGTTATTCCAAACAGGAG
AAGTTGACCATACTGGCTTAGGTACTGGTGATGAGGTTCTTGAACAGGCTAAGGCTTTAGAATTCGCAAATATCCAAATC
GAAACAGCACCGTCATTTAGTTATATTTATATGAATAATAATAAGCCTTACTTAAAGGATAAAAAGGTTCGCCAAGCACT
TATTTACGGATTAGACCGCAAAAAATATGTTGATACAGCGTTAAAAGGATACGGTACAGTAGCCAATGTACCAATTCATC
CAACTTCTTGGGCTTATACGGAAGAAGGAGTTAATAAATACGAATACGATAAAGAAAAAGCGAAAAAATTATTAGATGAA
GCAGGGTGGAAAGTTGGTTCGGATGGAATTCGTGAAAAAGATGGTCAAAAATTAAAACTTTCTTATTTCGGTCCAAGTTC
GGCTAAAGATAGTGATTTATTAATTCCGATTGCGAAAGAGAATTATAAAGAAATAGGTGTAGAGTTTAATCCTGAATTTA
TGGACTTTAATACGATGCTTTCTAAGGTAAACAAAGGGGATTACGATTTAGCTTCTGTTTCGACACCGATTACAAGTGAT
CCGAGTGAAACAGCTGGTGAATATTTGTCTACTGCAAATGAAACTAGTCTTGGTTATAAGAATGCAAAAGTAGATGAGCT
AATTCAAAAAGGTATTGAAACTGTAGATATCGAAAAGCGTAAACCTATTTATAAAGAATTGTATAAAGAATTAAGTGATG
ATCCACCAGTAATTCTATTAAACTACCGTAGAACAATTACTGGTTATAACGGGAATATAAAAGGAATTGATCCTGAAAAG
TACAACAGTATTAGTGCAAACTTACCAGTATTATCATTTGAAAAATAA

Upstream 100 bases:

>100_bases
TAAAACCGACTTTTCCGATTGGCTATAAATGTTGCTGTGGGAGGCATACATAAGGTAATTCGTTCAGAGGGTATAATCAT
AAAAAGTTAGGGTGGGGACA

Downstream 100 bases:

>100_bases
CGATAAGAATGTATAAGTAGAAGACATTTTTTCATTCGGTTAGTAATGTGCCGCTTTGAAAAAATGTTCTTCTATTTTAA
GCATAAGATTGGAGAGAAAA

Product: oligopeptide-binding protein OppA

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MKKKTKKWAGVFSVLMSSSLVLSACGGQEDAASTEPVKQQDLKNVKIEKIAATDKTKVPDKAKNRKDTLVVGISKPGGVF
LPYFQQNGWDGNVTSVIFASLVSTDKQGKPIPELAEKWDVSSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLTLLHDKAY
EGEVDISQYAVKGGKEYKEGKATSIEGIQVVDPQTIKITTEKVNSQAIFVLGGTVLSKAYYGKDYKQNTSLDYLKDLYGK
PLAAGPYKFEKYIPGQEVRFVANENYYAGKPKIQNFIYKITSGDTKLQLFQTGEVDHTGLGTGDEVLEQAKALEFANIQI
ETAPSFSYIYMNNNKPYLKDKKVRQALIYGLDRKKYVDTALKGYGTVANVPIHPTSWAYTEEGVNKYEYDKEKAKKLLDE
AGWKVGSDGIREKDGQKLKLSYFGPSSAKDSDLLIPIAKENYKEIGVEFNPEFMDFNTMLSKVNKGDYDLASVSTPITSD
PSETAGEYLSTANETSLGYKNAKVDELIQKGIETVDIEKRKPIYKELYKELSDDPPVILLNYRRTITGYNGNIKGIDPEK
YNSISANLPVLSFEK

Sequences:

>Translated_575_residues
MKKKTKKWAGVFSVLMSSSLVLSACGGQEDAASTEPVKQQDLKNVKIEKIAATDKTKVPDKAKNRKDTLVVGISKPGGVF
LPYFQQNGWDGNVTSVIFASLVSTDKQGKPIPELAEKWDVSSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLTLLHDKAY
EGEVDISQYAVKGGKEYKEGKATSIEGIQVVDPQTIKITTEKVNSQAIFVLGGTVLSKAYYGKDYKQNTSLDYLKDLYGK
PLAAGPYKFEKYIPGQEVRFVANENYYAGKPKIQNFIYKITSGDTKLQLFQTGEVDHTGLGTGDEVLEQAKALEFANIQI
ETAPSFSYIYMNNNKPYLKDKKVRQALIYGLDRKKYVDTALKGYGTVANVPIHPTSWAYTEEGVNKYEYDKEKAKKLLDE
AGWKVGSDGIREKDGQKLKLSYFGPSSAKDSDLLIPIAKENYKEIGVEFNPEFMDFNTMLSKVNKGDYDLASVSTPITSD
PSETAGEYLSTANETSLGYKNAKVDELIQKGIETVDIEKRKPIYKELYKELSDDPPVILLNYRRTITGYNGNIKGIDPEK
YNSISANLPVLSFEK
>Mature_575_residues
MKKKTKKWAGVFSVLMSSSLVLSACGGQEDAASTEPVKQQDLKNVKIEKIAATDKTKVPDKAKNRKDTLVVGISKPGGVF
LPYFQQNGWDGNVTSVIFASLVSTDKQGKPIPELAEKWDVSSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLTLLHDKAY
EGEVDISQYAVKGGKEYKEGKATSIEGIQVVDPQTIKITTEKVNSQAIFVLGGTVLSKAYYGKDYKQNTSLDYLKDLYGK
PLAAGPYKFEKYIPGQEVRFVANENYYAGKPKIQNFIYKITSGDTKLQLFQTGEVDHTGLGTGDEVLEQAKALEFANIQI
ETAPSFSYIYMNNNKPYLKDKKVRQALIYGLDRKKYVDTALKGYGTVANVPIHPTSWAYTEEGVNKYEYDKEKAKKLLDE
AGWKVGSDGIREKDGQKLKLSYFGPSSAKDSDLLIPIAKENYKEIGVEFNPEFMDFNTMLSKVNKGDYDLASVSTPITSD
PSETAGEYLSTANETSLGYKNAKVDELIQKGIETVDIEKRKPIYKELYKELSDDPPVILLNYRRTITGYNGNIKGIDPEK
YNSISANLPVLSFEK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=473, Percent_Identity=26.8498942917548, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1787762, Length=514, Percent_Identity=24.9027237354086, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1789887, Length=472, Percent_Identity=24.7881355932203, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1787495, Length=500, Percent_Identity=24.2, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI1787551, Length=465, Percent_Identity=24.0860215053763, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1787052, Length=508, Percent_Identity=25, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789397, Length=498, Percent_Identity=23.0923694779116, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI87081878, Length=464, Percent_Identity=23.0603448275862, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64011; Mature: 64011

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKTKKWAGVFSVLMSSSLVLSACGGQEDAASTEPVKQQDLKNVKIEKIAATDKTKVPD
CCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCC
KAKNRKDTLVVGISKPGGVFLPYFQQNGWDGNVTSVIFASLVSTDKQGKPIPELAEKWDV
HHCCCCCEEEEEECCCCCEEEEHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCC
SSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLTLLHDKAYEGEVDISQYAVKGGKEYKEG
CCCCEEEEEEEEECCCCCCCCCCCHHHHEEEEEEEECCCCCCCCCHHHHHHCCCCHHCCC
KATSIEGIQVVDPQTIKITTEKVNSQAIFVLGGTVLSKAYYGKDYKQNTSLDYLKDLYGK
CCCCCCCEEEECCCEEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHCC
PLAAGPYKFEKYIPGQEVRFVANENYYAGKPKIQNFIYKITSGDTKLQLFQTGEVDHTGL
CCCCCCCHHHHCCCCCCEEEEECCCEECCCCHHCEEEEEEECCCEEEEEEECCCCCCCCC
GTGDEVLEQAKALEFANIQIETAPSFSYIYMNNNKPYLKDKKVRQALIYGLDRKKYVDTA
CCHHHHHHHHHHEEEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LKGYGTVANVPIHPTSWAYTEEGVNKYEYDKEKAKKLLDEAGWKVGSDGIREKDGQKLKL
HHCCCCEEECCCCCCCCCCCHHCCCHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCEEEE
SYFGPSSAKDSDLLIPIAKENYKEIGVEFNPEFMDFNTMLSKVNKGDYDLASVSTPITSD
EEECCCCCCCCCEEEEECCCCHHHCCCEECCCEECHHHHHHHCCCCCCCHHHCCCCCCCC
PSETAGEYLSTANETSLGYKNAKVDELIQKGIETVDIEKRKPIYKELYKELSDDPPVILL
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCEEEE
NYRRTITGYNGNIKGIDPEKYNSISANLPVLSFEK
ECEEEEECCCCCCCCCCHHHCCCCCCCCCEEEECC
>Mature Secondary Structure
MKKKTKKWAGVFSVLMSSSLVLSACGGQEDAASTEPVKQQDLKNVKIEKIAATDKTKVPD
CCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCC
KAKNRKDTLVVGISKPGGVFLPYFQQNGWDGNVTSVIFASLVSTDKQGKPIPELAEKWDV
HHCCCCCEEEEEECCCCCEEEEHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCC
SSDQLTYTFHLRKDLKFSDGSPLTADDVAFTLTLLHDKAYEGEVDISQYAVKGGKEYKEG
CCCCEEEEEEEEECCCCCCCCCCCHHHHEEEEEEEECCCCCCCCCHHHHHHCCCCHHCCC
KATSIEGIQVVDPQTIKITTEKVNSQAIFVLGGTVLSKAYYGKDYKQNTSLDYLKDLYGK
CCCCCCCEEEECCCEEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHCC
PLAAGPYKFEKYIPGQEVRFVANENYYAGKPKIQNFIYKITSGDTKLQLFQTGEVDHTGL
CCCCCCCHHHHCCCCCCEEEEECCCEECCCCHHCEEEEEEECCCEEEEEEECCCCCCCCC
GTGDEVLEQAKALEFANIQIETAPSFSYIYMNNNKPYLKDKKVRQALIYGLDRKKYVDTA
CCHHHHHHHHHHEEEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LKGYGTVANVPIHPTSWAYTEEGVNKYEYDKEKAKKLLDEAGWKVGSDGIREKDGQKLKL
HHCCCCEEECCCCCCCCCCCHHCCCHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCEEEE
SYFGPSSAKDSDLLIPIAKENYKEIGVEFNPEFMDFNTMLSKVNKGDYDLASVSTPITSD
EEECCCCCCCCCEEEEECCCCHHHCCCEECCCEECHHHHHHHCCCCCCCHHHCCCCCCCC
PSETAGEYLSTANETSLGYKNAKVDELIQKGIETVDIEKRKPIYKELYKELSDDPPVILL
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCHHHHHHHHHCCCCCEEEE
NYRRTITGYNGNIKGIDPEKYNSISANLPVLSFEK
ECEEEEECCCCCCCCCCHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]