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The map label for this gene is appB [H]

Identifier: 218235203

GI number: 218235203

Start: 217679

End: 218602

Strand: Direct

Name: appB [H]

Synonym: BCB4264_A0246

Alternate gene names: 218235203

Gene position: 217679-218602 (Clockwise)

Preceding gene: 218235957

Following gene: 218234952

Centisome position: 4.02

GC content: 35.06

Gene sequence:

>924_bases
ATGATTCCTACACTGCTTGGTACATCAATTATTATCTTCGTCCTGTTCGCACTTCTTCCTGGGGATTATATTGATTCAAA
CCCGAAAATTACACCGGAAAGAGCAGCTGAACTGAGGGAGTTATATGGGTTGAACAAACCAATTGTTGAGCGTTATTTTC
ATTGGTTAGGTAATGCATTGCAAGGGGACTTTGGCTTCTCGCTTCAATATCAAGAGCCTGTAACGTCATTATTAAATAAA
TTTATTTGGAATTCTTTTAGTGTAGCAATTATTGCATTGTTTTTTATTTGGCTAATTGCACTTATTATCGGTGTATTCTC
GGCGACGAAGCAACATTCTTTATTCGATAAACTTGTAACAATCGGTGTTTTTGCAGCAATGTCATTCCCTTCATTCTTTA
TCGGTTTGTTTTTAATTAAGGTGTTTGCGGTTGACTTTAAGTTATTACCGATAGGTGGAATGATTGATGTTGGCAGTAAT
TCAACAGGATTTGCTTACGTGATAGAAGTTGCAAAACATATGATTTTACCAGTATTTATTTTGACACTTCTTGGGGTCGG
CTCGCTAACTCGTTATTTTAGAACAAGTATGTTAGAGGTTGTAAGACAAGATTACATACGAACAGCAAGAGCGAAAGGTT
TGAAGGAGAAGACGGTTATTTATAAGCATGCATTAAAAAATGCAATTTTACCGGCAATTACGTTATTAGCATTTGAATTA
CCTGGATTGTTTTCTGGGGCAATTATTATTGAACAAATTTTTAACTGGCCAGGGATTGGTAATATTCAATTAGAAGCATT
AAATTTCCGTGACTATACAGTATTGATGGCATTTACGATGTTTCTTTCTTGTTTAACAATCGTTTCAAACTTTTTAGCAG
ATATCGTATATGCAGTTGTTGATCCAAGAATTCGGTTGAAGTAA

Upstream 100 bases:

>100_bases
CGGTTAGTAATGTGCCGCTTTGAAAAAATGTTCTTCTATTTTAAGCATAAGATTGGAGAGAAAAAGGTTGAAAACATATA
TTGTTCGTAGGTTGTTACAA

Downstream 100 bases:

>100_bases
GGGGCAAAGAAATGGAGACTGTTACAGTAATTACAGAAAAAAAAGAAAAAAAGAGAAGAAATGAATCATCACCATGGCGA
CAAGCTTTTAAAAAGATAAA

Product: oligopeptide ABC transporter, permease protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIPTLLGTSIIIFVLFALLPGDYIDSNPKITPERAAELRELYGLNKPIVERYFHWLGNALQGDFGFSLQYQEPVTSLLNK
FIWNSFSVAIIALFFIWLIALIIGVFSATKQHSLFDKLVTIGVFAAMSFPSFFIGLFLIKVFAVDFKLLPIGGMIDVGSN
STGFAYVIEVAKHMILPVFILTLLGVGSLTRYFRTSMLEVVRQDYIRTARAKGLKEKTVIYKHALKNAILPAITLLAFEL
PGLFSGAIIIEQIFNWPGIGNIQLEALNFRDYTVLMAFTMFLSCLTIVSNFLADIVYAVVDPRIRLK

Sequences:

>Translated_307_residues
MIPTLLGTSIIIFVLFALLPGDYIDSNPKITPERAAELRELYGLNKPIVERYFHWLGNALQGDFGFSLQYQEPVTSLLNK
FIWNSFSVAIIALFFIWLIALIIGVFSATKQHSLFDKLVTIGVFAAMSFPSFFIGLFLIKVFAVDFKLLPIGGMIDVGSN
STGFAYVIEVAKHMILPVFILTLLGVGSLTRYFRTSMLEVVRQDYIRTARAKGLKEKTVIYKHALKNAILPAITLLAFEL
PGLFSGAIIIEQIFNWPGIGNIQLEALNFRDYTVLMAFTMFLSCLTIVSNFLADIVYAVVDPRIRLK
>Mature_307_residues
MIPTLLGTSIIIFVLFALLPGDYIDSNPKITPERAAELRELYGLNKPIVERYFHWLGNALQGDFGFSLQYQEPVTSLLNK
FIWNSFSVAIIALFFIWLIALIIGVFSATKQHSLFDKLVTIGVFAAMSFPSFFIGLFLIKVFAVDFKLLPIGGMIDVGSN
STGFAYVIEVAKHMILPVFILTLLGVGSLTRYFRTSMLEVVRQDYIRTARAKGLKEKTVIYKHALKNAILPAITLLAFEL
PGLFSGAIIIEQIFNWPGIGNIQLEALNFRDYTVLMAFTMFLSCLTIVSNFLADIVYAVVDPRIRLK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=326, Percent_Identity=34.6625766871166, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787053, Length=309, Percent_Identity=31.7152103559871, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1787497, Length=308, Percent_Identity=34.7402597402597, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1789888, Length=310, Percent_Identity=34.1935483870968, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1787761, Length=316, Percent_Identity=30.6962025316456, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788504, Length=295, Percent_Identity=31.5254237288136, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1787550, Length=268, Percent_Identity=26.865671641791, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34423; Mature: 34423

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPTLLGTSIIIFVLFALLPGDYIDSNPKITPERAAELRELYGLNKPIVERYFHWLGNAL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QGDFGFSLQYQEPVTSLLNKFIWNSFSVAIIALFFIWLIALIIGVFSATKQHSLFDKLVT
CCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGVFAAMSFPSFFIGLFLIKVFAVDFKLLPIGGMIDVGSNSTGFAYVIEVAKHMILPVFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
LTLLGVGSLTRYFRTSMLEVVRQDYIRTARAKGLKEKTVIYKHALKNAILPAITLLAFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PGLFSGAIIIEQIFNWPGIGNIQLEALNFRDYTVLMAFTMFLSCLTIVSNFLADIVYAVV
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DPRIRLK
CCCCCCC
>Mature Secondary Structure
MIPTLLGTSIIIFVLFALLPGDYIDSNPKITPERAAELRELYGLNKPIVERYFHWLGNAL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QGDFGFSLQYQEPVTSLLNKFIWNSFSVAIIALFFIWLIALIIGVFSATKQHSLFDKLVT
CCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGVFAAMSFPSFFIGLFLIKVFAVDFKLLPIGGMIDVGSNSTGFAYVIEVAKHMILPVFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
LTLLGVGSLTRYFRTSMLEVVRQDYIRTARAKGLKEKTVIYKHALKNAILPAITLLAFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PGLFSGAIIIEQIFNWPGIGNIQLEALNFRDYTVLMAFTMFLSCLTIVSNFLADIVYAVV
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DPRIRLK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]