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The map label for this gene is glpF [H]

Identifier: 218235696

GI number: 218235696

Start: 1039068

End: 1039889

Strand: Direct

Name: glpF [H]

Synonym: BCB4264_A1065

Alternate gene names: 218235696

Gene position: 1039068-1039889 (Clockwise)

Preceding gene: 218232750

Following gene: 218235134

Centisome position: 19.17

GC content: 39.42

Gene sequence:

>822_bases
ATGTCAGCATTTTTAGGAGAGTTAATAGGGACTGCGTTGTTAATCGTACTTGGTGGCGGCGTTTGTGCTGGTGTAAGTTT
AAAGAAATCGTTTGCGAAAGATTCAGGTTGGATTGTTATTACAATGGGCTGGGGCTTAGCAGTAGCGGTTGCAGCGTATG
CGGTTGGATCAATTAGTGGGGCACATTTGAATCCAGCTTTAACGATAGGATTAGCATTTAAGGGAGCGTTCCCATGGAGT
GACGTACCAGGTTATATCGTAGCACAAATGATTGGGGCAATTATCGGGGCAGTTATCGTATATTTACATTACTTACCACA
CTGGAAAGAAACAGAAGATCCAGGAACAAAGTTAGGTGTATTTGCAACAGGTCCAGCAATTCCGAACACATTTGCAAACC
TTTTAAGTGAAATGATTGGAACATTCGTTTTAGTATTTGGTATATTAGCAATTGGTGCAAATAAATTTGCAGATGGATTA
AATCCATTTATCGTAGGTTTCTTAATTGTAAGTATTGGTTTATCATTAGGTGGGACAACAGGATACGCAATTAACCCAGC
GCGTGATTTAGGTCCGCGTATTGCACACTTCTTCCTTCCGATTGCAGGAAAAGGCGGTTCAAACTGGAAGTATGCATGGA
TTCCAGTAGTTGGTCCAATTTTAGGTGGATCACTTGCAGGTTTATTCCATCAAGTTGTATTTGAGGGAAAACAAAATTCA
GCACTTATTTATGTGATTATCGCAACTGTGATTGTACTAGCAATCTCTTATATGACAAGTAAAAAAAGCGGAAGCAATAC
AAATAGTAGAAAAGTAGCATAA

Upstream 100 bases:

>100_bases
CGTGCAAGCTGTGGGTCTTTTTCTTTTGACAAAAACGAATGGCCATTCATTTTACCTCTATCACATTTGAAAGCGTTTAA
ATTGATTGTGGAGGGATACT

Downstream 100 bases:

>100_bases
GGGGGAAACCGATATGAAAAAATATATTCTTTCATTAGACCAAGGAACAACAAGCTCACGCGCAATTCTTTTCAATAAAA
AAGGTGAAATTGTTCATTCA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISGAHLNPALTIGLAFKGAFPWS
DVPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGL
NPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNS
ALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA

Sequences:

>Translated_273_residues
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISGAHLNPALTIGLAFKGAFPWS
DVPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGL
NPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNS
ALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA
>Mature_272_residues
SAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISGAHLNPALTIGLAFKGAFPWSD
VPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLN
PFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNSA
LIYVIIATVIVLAISYMTSKKSGSNTNSRKVA

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=242, Percent_Identity=38.0165289256198, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI157266307, Length=250, Percent_Identity=36.4, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI4502187, Length=249, Percent_Identity=34.9397590361446, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=32.9268292682927, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4502181, Length=206, Percent_Identity=34.4660194174757, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4755125, Length=206, Percent_Identity=34.4660194174757, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI37694062, Length=246, Percent_Identity=30.8943089430894, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI310133356, Length=200, Percent_Identity=30.5, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310114181, Length=200, Percent_Identity=30.5, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790362, Length=259, Percent_Identity=34.7490347490347, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1787101, Length=189, Percent_Identity=33.8624338624339, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994009, Length=230, Percent_Identity=33.9130434782609, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17544068, Length=246, Percent_Identity=35.7723577235772, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32564052, Length=240, Percent_Identity=30.8333333333333, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17533613, Length=240, Percent_Identity=30.8333333333333, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71992966, Length=279, Percent_Identity=28.3154121863799, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531431, Length=259, Percent_Identity=30.5019305019305, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17531429, Length=259, Percent_Identity=30.5019305019305, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=236, Percent_Identity=28.8135593220339, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=269, Percent_Identity=27.1375464684015, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=195, Percent_Identity=29.7435897435897, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=36.4077669902913, Blast_Score=115, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322985, Length=290, Percent_Identity=26.8965517241379, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24652747, Length=241, Percent_Identity=32.3651452282158, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45551084, Length=241, Percent_Identity=32.3651452282158, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24762342, Length=249, Percent_Identity=28.5140562248996, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24762344, Length=249, Percent_Identity=31.7269076305221, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24762346, Length=249, Percent_Identity=31.7269076305221, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24762348, Length=249, Percent_Identity=31.7269076305221, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20130305, Length=249, Percent_Identity=31.7269076305221, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922828, Length=194, Percent_Identity=30.9278350515464, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28278; Mature: 28146

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISG
CCHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTIGLAFKGAFPWSDVPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGV
CCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLSLGGTT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNS
CCEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
ALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISG
CHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTIGLAFKGAFPWSDVPGYIVAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGV
CCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLSLGGTT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFFLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNS
CCEECCHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
ALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]