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The map label for this gene is glyA

Identifier: 213692714

GI number: 213692714

Start: 2014787

End: 2016094

Strand: Reverse

Name: glyA

Synonym: Blon_1847

Alternate gene names: 213692714

Gene position: 2016094-2014787 (Counterclockwise)

Preceding gene: 213692716

Following gene: 213692713

Centisome position: 71.17

GC content: 65.29

Gene sequence:

>1308_bases
ATGACCGCTTCACCCCTTGCGCAGAAAGCGACCGATGCTTTCAACGCGCCGATCTGCGAAACCGATCCTGAGATCGCCGA
ACTGCTGGACTCCGAACTGGGCCGCCAGCGCAATGGCCTTGAGATGATCGCCTCTGAGAACTTCGTGCCGCGAGCGGTAC
TGCAATGCCAGGGCTCCGTGCTGACCAACAAATACGCCGAAGGCTACCCCGGCCACCGCTACTATGGCGGCTGTGAATAC
GTGGACCAGATCGAAACCATCGCCCGCGAACGCGCCAAGGCCCTGTTCGGCGCCGAATACGTCAACGTCCAGCCGCACTC
CGGCGCCCAGGCCAACGCCGCCGTCTACCAGGCGCTCGTCAAGCCCGGCGACACCGTGCTCGGTCTGGCCCTCGACCACG
GCGGCCACCTGACCCACGGCATGAAAATCAACTTCTCTGGCCGTTTCTACCACGCCGAGGCGTACGGCGTGAACCCCGTG
ACCTTCCGCATCGATCCGGAAATCATCCGCCAGCGCGCGCTCGAAACCCACCCGGCCATGATCATCGGCGGCTGGTCCGC
CTACCCGCGCATCGAGGACTTCAAGGCCATGAAGGAGATCGCCGACGAGGTCGGCGCCAAATTCTGGGTCGACATGGCCC
ACTTCGCCGGACTCGTGGCCGCAGGCCTGCACCCGAGCCCCGTGCCATACGCCGACGTGGTCTCTTCCACCTCGCACAAG
ACCTTCGGCGGTCCGCGTTCCGGTTTCATTCTCGCCAAGCAGGAATACGCCAAGAAACTCAACTCCTCCGTGTTCCCCGG
CCAGCAGGGCGGTCCGCTCATGCACGTGATCGCGGGCAAGGCCGTGAGCTTCAAGGTGGCCGGCACCCCCGAATTCAAAG
ACCGTATGCAGCGCACGCTCGACGGCGCCAAGATTCTCGCTGAACGTCTCCTGGCCGACGATGTCAAGGCCAACGGCATC
AGCGTGCTGACCGGCGGCACTGACGTGCACCTGGTGATGGTCGACCTGCGCAACAGCGAGATGGACGGGCAGCAGGGCGA
GGATTTGCTTGCCGCCTGCGGCATCACCATCAACCGCAACACCGTGCCCTTCGACCCGCGCCCGGCCTCGGTGGCCTCGG
GCCTGAGAATCGGCACGTCCGCCCTGGCCACGCGCGGTTTCGGACCGAAGGAATACGAGGAGGTGGCCGACATCATCGGC
ACCGCGCTCGCCGCCGGCCCGTCCGCCGACGTGACCGCGCTGAAGGCCCGCGTCGACAAGCTCGCCGAAGACTTCCCGCT
CTACCCCGACCTGGACCAGATTCACTGA

Upstream 100 bases:

>100_bases
AACGGGGACCGGCCATATCATGAAACAGGCCGGTTACAAACGGGCCATACAGTAAGGCGACAGCGGCGAATCAATTGTCG
CACATACCAAAGGAGCACCC

Downstream 100 bases:

>100_bases
TTTGCTGATTCGCTAATCCAGCGTGGTGTACCGGTTAGTGATGATTCGCTGACCGGAGCGCATCACATCACCGGCTTCCC
GCCTGAGATGGAGGGGAGCC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTASPLAQKATDAFNAPICETDPEIAELLDSELGRQRNGLEMIASENFVPRAVLQCQGSVLTNKYAEGYPGHRYYGGCEY
VDQIETIARERAKALFGAEYVNVQPHSGAQANAAVYQALVKPGDTVLGLALDHGGHLTHGMKINFSGRFYHAEAYGVNPV
TFRIDPEIIRQRALETHPAMIIGGWSAYPRIEDFKAMKEIADEVGAKFWVDMAHFAGLVAAGLHPSPVPYADVVSSTSHK
TFGGPRSGFILAKQEYAKKLNSSVFPGQQGGPLMHVIAGKAVSFKVAGTPEFKDRMQRTLDGAKILAERLLADDVKANGI
SVLTGGTDVHLVMVDLRNSEMDGQQGEDLLAACGITINRNTVPFDPRPASVASGLRIGTSALATRGFGPKEYEEVADIIG
TALAAGPSADVTALKARVDKLAEDFPLYPDLDQIH

Sequences:

>Translated_435_residues
MTASPLAQKATDAFNAPICETDPEIAELLDSELGRQRNGLEMIASENFVPRAVLQCQGSVLTNKYAEGYPGHRYYGGCEY
VDQIETIARERAKALFGAEYVNVQPHSGAQANAAVYQALVKPGDTVLGLALDHGGHLTHGMKINFSGRFYHAEAYGVNPV
TFRIDPEIIRQRALETHPAMIIGGWSAYPRIEDFKAMKEIADEVGAKFWVDMAHFAGLVAAGLHPSPVPYADVVSSTSHK
TFGGPRSGFILAKQEYAKKLNSSVFPGQQGGPLMHVIAGKAVSFKVAGTPEFKDRMQRTLDGAKILAERLLADDVKANGI
SVLTGGTDVHLVMVDLRNSEMDGQQGEDLLAACGITINRNTVPFDPRPASVASGLRIGTSALATRGFGPKEYEEVADIIG
TALAAGPSADVTALKARVDKLAEDFPLYPDLDQIH
>Mature_434_residues
TASPLAQKATDAFNAPICETDPEIAELLDSELGRQRNGLEMIASENFVPRAVLQCQGSVLTNKYAEGYPGHRYYGGCEYV
DQIETIARERAKALFGAEYVNVQPHSGAQANAAVYQALVKPGDTVLGLALDHGGHLTHGMKINFSGRFYHAEAYGVNPVT
FRIDPEIIRQRALETHPAMIIGGWSAYPRIEDFKAMKEIADEVGAKFWVDMAHFAGLVAAGLHPSPVPYADVVSSTSHKT
FGGPRSGFILAKQEYAKKLNSSVFPGQQGGPLMHVIAGKAVSFKVAGTPEFKDRMQRTLDGAKILAERLLADDVKANGIS
VLTGGTDVHLVMVDLRNSEMDGQQGEDLLAACGITINRNTVPFDPRPASVASGLRIGTSALATRGFGPKEYEEVADIIGT
ALAAGPSADVTALKARVDKLAEDFPLYPDLDQIH

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=462, Percent_Identity=41.1255411255411, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI261862352, Length=462, Percent_Identity=41.1255411255411, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI261862350, Length=462, Percent_Identity=41.1255411255411, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI261862348, Length=462, Percent_Identity=41.1255411255411, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI261862346, Length=462, Percent_Identity=40.2597402597403, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI22547186, Length=433, Percent_Identity=42.7251732101617, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI22547189, Length=418, Percent_Identity=41.1483253588517, Blast_Score=278, Evalue=8e-75,
Organism=Escherichia coli, GI1788902, Length=415, Percent_Identity=51.566265060241, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25144732, Length=403, Percent_Identity=43.1761786600496, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI25144729, Length=403, Percent_Identity=43.1761786600496, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6319739, Length=422, Percent_Identity=41.2322274881517, Blast_Score=318, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6323087, Length=410, Percent_Identity=39.7560975609756, Blast_Score=305, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24640005, Length=449, Percent_Identity=43.652561247216, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI221329721, Length=449, Percent_Identity=43.652561247216, Blast_Score=352, Evalue=4e-97,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_BIFLI (B7GTL3)

Other databases:

- EMBL:   CP001095
- RefSeq:   YP_002323300.1
- ProteinModelPortal:   B7GTL3
- GeneID:   7055605
- GenomeReviews:   CP001095_GR
- KEGG:   bln:Blon_1847
- HOGENOM:   HBG301263
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46732; Mature: 46601

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPLAQKATDAFNAPICETDPEIAELLDSELGRQRNGLEMIASENFVPRAVLQCQGSV
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHCCCH
LTNKYAEGYPGHRYYGGCEYVDQIETIARERAKALFGAEYVNVQPHSGAQANAAVYQALV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
KPGDTVLGLALDHGGHLTHGMKINFSGRFYHAEAYGVNPVTFRIDPEIIRQRALETHPAM
CCCCEEEEEEECCCCCEECCEEEEECCEEEEEEEECCCCEEEEECHHHHHHHHHHCCCCE
IIGGWSAYPRIEDFKAMKEIADEVGAKFWVDMAHFAGLVAAGLHPSPVPYADVVSSTSHK
EEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCC
TFGGPRSGFILAKQEYAKKLNSSVFPGQQGGPLMHVIAGKAVSFKVAGTPEFKDRMQRTL
CCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHH
DGAKILAERLLADDVKANGISVLTGGTDVHLVMVDLRNSEMDGQQGEDLLAACGITINRN
HHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCHHHHHCCCEECCC
TVPFDPRPASVASGLRIGTSALATRGFGPKEYEEVADIIGTALAAGPSADVTALKARVDK
CCCCCCCCHHHHCCCHHCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LAEDFPLYPDLDQIH
HHHHCCCCCCHHHCC
>Mature Secondary Structure 
TASPLAQKATDAFNAPICETDPEIAELLDSELGRQRNGLEMIASENFVPRAVLQCQGSV
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHCCCH
LTNKYAEGYPGHRYYGGCEYVDQIETIARERAKALFGAEYVNVQPHSGAQANAAVYQALV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH
KPGDTVLGLALDHGGHLTHGMKINFSGRFYHAEAYGVNPVTFRIDPEIIRQRALETHPAM
CCCCEEEEEEECCCCCEECCEEEEECCEEEEEEEECCCCEEEEECHHHHHHHHHHCCCCE
IIGGWSAYPRIEDFKAMKEIADEVGAKFWVDMAHFAGLVAAGLHPSPVPYADVVSSTSHK
EEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCC
TFGGPRSGFILAKQEYAKKLNSSVFPGQQGGPLMHVIAGKAVSFKVAGTPEFKDRMQRTL
CCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHH
DGAKILAERLLADDVKANGISVLTGGTDVHLVMVDLRNSEMDGQQGEDLLAACGITINRN
HHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCHHHHHCCCEECCC
TVPFDPRPASVASGLRIGTSALATRGFGPKEYEEVADIIGTALAAGPSADVTALKARVDK
CCCCCCCCHHHHCCCHHCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LAEDFPLYPDLDQIH
HHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA