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The map label for this gene is miaB [H]

Identifier: 213691832

GI number: 213691832

Start: 1111125

End: 1112579

Strand: Direct

Name: miaB [H]

Synonym: Blon_0941

Alternate gene names: 213691832

Gene position: 1111125-1112579 (Clockwise)

Preceding gene: 213691830

Following gene: 213691833

Centisome position: 39.22

GC content: 61.37

Gene sequence:

>1455_bases
ATGAATGAAGACATGATGACCGAGGCGGAGCGTGCCTCGATTGCAGCCGATGGAACCGATAGCTTGCTGGGGGAGCGTGG
AAAGGGCGTATTCCATATCCACACGCTCGGCTGCCAGATGAACGTGCACGATTCCGAGCGCATCGCCGGCGTGCTGGAGG
CCAATGGCTACGTGCCTGCAACCGAGGACCAGATCAATGACAATGACCTCGATCTGCTGGTGCTCAATACCTGTGCGGTA
CGCGAGAACGCAGCCGAACGCATGTATGGCACCATTGGCCGATTCAATCGCGTCAAACTGGTTCGGCCGAACCTGCAGAT
CGCCGTTGGCGGCTGCATGGCCCAGCTTGACCGCAAGAAGATCGCGGATACTGCGCCATGGGTTTCCGCGGTGTTCGGTA
CCAAGAACATCGAAGATCTGCCCAAGCTATTGGATCAGAATCGTGCGACCGGCAAGGCGCAGGTCCAGGTCACTGAACAA
TTGCGGCAGTTCCCGAGCCAGCTTCCCGCCGCCCGCGCCTCCCGCATCAGCTCGTGGGTGGCGATTTCCGTGGGCTGCAA
TAACACCTGCACGTTCTGCATCGTGCCCACCACGCGAGGCAAGGAAAAGGACCGCCGCCCCGGTGACATTCTTGATGAGA
TCCGCCAATGCGTGGCCGGCGGCGCCAAGGAGGTCACGCTTCTCGGCCAGAACGTGAACTCGTTTGGTTATGGCATCGGC
GATCGATACGCTTTCAGCAAGCTGTTGCGTGCCTGCGGAACCATCGACGGTCTCGAACGTGTGCGCTTCACTTCGCCGCA
CCCGGCCGCCTTCACCGATGATGTGATTGCAGCCATGGCCGAAACGCCGAATATCATGCATCAACTGCACTTCCCGCTGC
AGTCCGGTTCCGACCGCATCCTGCGCGCGATGCGCCGCTCCTACAGGTCCGCCAAGTTCCTCGATATCTTGGGCCGCATC
CGCGAGGCCATGCCCGACGCACAGATTTCCACCGATATCATCGTCGGATTCCCCGGCGAAACCGAGGAGGACTTCCAGCA
GACCATGGACGTGGTGCGCCAGGCGCGCTTCTCCTCGGCGTTCACGTTCATCTACTCGCCACGCCCCGGCACGCCGGCCG
CCGCAATGGAACAGATTCCGCGCGACGTGGTGCAGGACCGGTTCGACCGACTGGTGGCGTTGCAGGAGCAGATCACCGAA
GAGAACCTCGCCACATTCGAGGGCCGCGACGTCGAAGTGATGATCACCGGCAAACTCGGCAAGAAGGACACCGACACCCA
CCGTGTCACCGGCCGCGAGAAAACTGGCGTGCTGGTGCACATCGGCGTGCCTGAAGGCGAACCGGTACCTGAAATCGGCG
ATTTCGTCACGGTCACCGTCACACACGCTGGCCGCCATAATCTGCTTGCCGACCCCGATGTGGCCGCTGGACAGACCTAT
TCCGTGCGGCACTGA

Upstream 100 bases:

>100_bases
TTAGCGGGGAATCTTTCCAAGAACCCATAATTTGACATCGTGACAACCAATGGCATACGTGACCGGAGCATGACGAACCC
CGCCGATAGAATCGGTCGTT

Downstream 100 bases:

>100_bases
GGGGGCGTATATGACGCAACGAGTCGTCTCCATCGTCGGTCCCACCGCGTCAGGCAAGACGGGGTTGGGCATCGCCATCG
CCCGGCGTCTGGCCGAAGCG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNEDMMTEAERASIAADGTDSLLGERGKGVFHIHTLGCQMNVHDSERIAGVLEANGYVPATEDQINDNDLDLLVLNTCAV
RENAAERMYGTIGRFNRVKLVRPNLQIAVGGCMAQLDRKKIADTAPWVSAVFGTKNIEDLPKLLDQNRATGKAQVQVTEQ
LRQFPSQLPAARASRISSWVAISVGCNNTCTFCIVPTTRGKEKDRRPGDILDEIRQCVAGGAKEVTLLGQNVNSFGYGIG
DRYAFSKLLRACGTIDGLERVRFTSPHPAAFTDDVIAAMAETPNIMHQLHFPLQSGSDRILRAMRRSYRSAKFLDILGRI
REAMPDAQISTDIIVGFPGETEEDFQQTMDVVRQARFSSAFTFIYSPRPGTPAAAMEQIPRDVVQDRFDRLVALQEQITE
ENLATFEGRDVEVMITGKLGKKDTDTHRVTGREKTGVLVHIGVPEGEPVPEIGDFVTVTVTHAGRHNLLADPDVAAGQTY
SVRH

Sequences:

>Translated_484_residues
MNEDMMTEAERASIAADGTDSLLGERGKGVFHIHTLGCQMNVHDSERIAGVLEANGYVPATEDQINDNDLDLLVLNTCAV
RENAAERMYGTIGRFNRVKLVRPNLQIAVGGCMAQLDRKKIADTAPWVSAVFGTKNIEDLPKLLDQNRATGKAQVQVTEQ
LRQFPSQLPAARASRISSWVAISVGCNNTCTFCIVPTTRGKEKDRRPGDILDEIRQCVAGGAKEVTLLGQNVNSFGYGIG
DRYAFSKLLRACGTIDGLERVRFTSPHPAAFTDDVIAAMAETPNIMHQLHFPLQSGSDRILRAMRRSYRSAKFLDILGRI
REAMPDAQISTDIIVGFPGETEEDFQQTMDVVRQARFSSAFTFIYSPRPGTPAAAMEQIPRDVVQDRFDRLVALQEQITE
ENLATFEGRDVEVMITGKLGKKDTDTHRVTGREKTGVLVHIGVPEGEPVPEIGDFVTVTVTHAGRHNLLADPDVAAGQTY
SVRH
>Mature_484_residues
MNEDMMTEAERASIAADGTDSLLGERGKGVFHIHTLGCQMNVHDSERIAGVLEANGYVPATEDQINDNDLDLLVLNTCAV
RENAAERMYGTIGRFNRVKLVRPNLQIAVGGCMAQLDRKKIADTAPWVSAVFGTKNIEDLPKLLDQNRATGKAQVQVTEQ
LRQFPSQLPAARASRISSWVAISVGCNNTCTFCIVPTTRGKEKDRRPGDILDEIRQCVAGGAKEVTLLGQNVNSFGYGIG
DRYAFSKLLRACGTIDGLERVRFTSPHPAAFTDDVIAAMAETPNIMHQLHFPLQSGSDRILRAMRRSYRSAKFLDILGRI
REAMPDAQISTDIIVGFPGETEEDFQQTMDVVRQARFSSAFTFIYSPRPGTPAAAMEQIPRDVVQDRFDRLVALQEQITE
ENLATFEGRDVEVMITGKLGKKDTDTHRVTGREKTGVLVHIGVPEGEPVPEIGDFVTVTVTHAGRHNLLADPDVAAGQTY
SVRH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=488, Percent_Identity=33.6065573770492, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=33.6065573770492, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI93277076, Length=362, Percent_Identity=30.6629834254144, Blast_Score=167, Evalue=3e-41,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=39.2461197339246, Blast_Score=310, Evalue=9e-86,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=30.7692307692308, Blast_Score=198, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=30.8333333333333, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI71996771, Length=380, Percent_Identity=27.8947368421053, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI21356207, Length=480, Percent_Identity=32.7083333333333, Blast_Score=253, Evalue=3e-67,
Organism=Drosophila melanogaster, GI19922432, Length=371, Percent_Identity=30.7277628032345, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53221; Mature: 53221

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEDMMTEAERASIAADGTDSLLGERGKGVFHIHTLGCQMNVHDSERIAGVLEANGYVPA
CCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEEEEEECCCCHHHHHEEEECCCCCCC
TEDQINDNDLDLLVLNTCAVRENAAERMYGTIGRFNRVKLVRPNLQIAVGGCMAQLDRKK
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHH
IADTAPWVSAVFGTKNIEDLPKLLDQNRATGKAQVQVTEQLRQFPSQLPAARASRISSWV
HHCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCHHHHHHCCCEE
AISVGCNNTCTFCIVPTTRGKEKDRRPGDILDEIRQCVAGGAKEVTLLGQNVNSFGYGIG
EEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHCCCCC
DRYAFSKLLRACGTIDGLERVRFTSPHPAAFTDDVIAAMAETPNIMHQLHFPLQSGSDRI
HHHHHHHHHHHHCCHHHHHHCEECCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHH
LRAMRRSYRSAKFLDILGRIREAMPDAQISTDIIVGFPGETEEDFQQTMDVVRQARFSSA
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCE
FTFIYSPRPGTPAAAMEQIPRDVVQDRFDRLVALQEQITEENLATFEGRDVEVMITGKLG
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCC
KKDTDTHRVTGREKTGVLVHIGVPEGEPVPEIGDFVTVTVTHAGRHNLLADPDVAAGQTY
CCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEE
SVRH
ECCC
>Mature Secondary Structure
MNEDMMTEAERASIAADGTDSLLGERGKGVFHIHTLGCQMNVHDSERIAGVLEANGYVPA
CCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEEEEEECCCCHHHHHEEEECCCCCCC
TEDQINDNDLDLLVLNTCAVRENAAERMYGTIGRFNRVKLVRPNLQIAVGGCMAQLDRKK
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHH
IADTAPWVSAVFGTKNIEDLPKLLDQNRATGKAQVQVTEQLRQFPSQLPAARASRISSWV
HHCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCHHHHHHCCCEE
AISVGCNNTCTFCIVPTTRGKEKDRRPGDILDEIRQCVAGGAKEVTLLGQNVNSFGYGIG
EEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHCCCCC
DRYAFSKLLRACGTIDGLERVRFTSPHPAAFTDDVIAAMAETPNIMHQLHFPLQSGSDRI
HHHHHHHHHHHHCCHHHHHHCEECCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHH
LRAMRRSYRSAKFLDILGRIREAMPDAQISTDIIVGFPGETEEDFQQTMDVVRQARFSSA
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCE
FTFIYSPRPGTPAAAMEQIPRDVVQDRFDRLVALQEQITEENLATFEGRDVEVMITGKLG
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCC
KKDTDTHRVTGREKTGVLVHIGVPEGEPVPEIGDFVTVTVTHAGRHNLLADPDVAAGQTY
CCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEE
SVRH
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA