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The map label for this gene is miaA [H]

Identifier: 213691833

GI number: 213691833

Start: 1112590

End: 1113576

Strand: Direct

Name: miaA [H]

Synonym: Blon_0942

Alternate gene names: 213691833

Gene position: 1112590-1113576 (Clockwise)

Preceding gene: 213691832

Following gene: 213691835

Centisome position: 39.28

GC content: 63.63

Gene sequence:

>987_bases
ATGACGCAACGAGTCGTCTCCATCGTCGGTCCCACCGCGTCAGGCAAGACGGGGTTGGGCATCGCCATCGCCCGGCGTCT
GGCCGAAGCGGGGGAGCGGGCCGAAATCGTGAACGCGGACGCCTACCAGATGTACCGTGGCATGGATATCGGCACCGCCA
AACCCACCGCCGAAGAACAGGCGGTCGTGTCGCACCATCTCATCGACATCATCGACCCCGAAGACACGATGAGCGTGGCC
CGATTCCAGCAGCTTGCGCGCGAGACCATCGCCGACCTGCAATCACGTGGCATCCGGCCGATTCTCGTCGGCGGCTCCGG
CTTGTACGCCAGAGCGGCCATCGATGACATCACCTTCCCCGGCACCGATCCCGACGTGCGCACACGTCTTGAGGAACGCG
AGAAAACCGAGGGTGCCGGTGCTTTGTTCGACGAGCTGCGGGCCAAAGACCCCGAGGCCGCCGCGCGCATGGACCCGCGC
AATCCACGCCGCACCATCCGTGCGCTGGAAGTCATCGAGCTTACCGGCAAACCGTATTCGGCCAGTCTGCCGCGCTACCG
GTACGTGATTCCGTCCGTGCAGATCGGACTCGACCTGGATCGCCCGGATTTGGACCATCGCATCGATTTGCGCACCAAGC
AGATGTATGACGATGGGTTCATCGAAGAAGTCGAACGGCTGCGCCCGCATCTGGGTGCCACCGCCGTACGCGCGCTCGGC
TATCAGCAGATCATCGACCTGCTGGACGGCATCTGGGATGTGAACGACGCCTTCGCGGACATCGCACAGAAAACCAAGCG
ACTCGCCCGCAAACAGATGGGATGGTTCGGCCGTGACCCGCGCATCCACTGGCTGCAGGCCCTGAACCCGAAACTGGTCG
ACAACGCGATGGCCATTATCGCGCACGCCGACGCCGGCGACTACGACTCGATCGACGCCCGAGCCGACGAATACACCCAG
CATCATCTGGGGGATATCACGGCGTAG

Upstream 100 bases:

>100_bases
GTCACGGTCACCGTCACACACGCTGGCCGCCATAATCTGCTTGCCGACCCCGATGTGGCCGCTGGACAGACCTATTCCGT
GCGGCACTGAGGGGGCGTAT

Downstream 100 bases:

>100_bases
GAATCAGCCAAAGGCCGATGCAATCACCCGCAATCAGTGTGCAGGCCCCTGATTCCAGCCGGCGATCAAGAACACGCGCG
TCGGATTCGCCGGGCGTAGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTQRVVSIVGPTASGKTGLGIAIARRLAEAGERAEIVNADAYQMYRGMDIGTAKPTAEEQAVVSHHLIDIIDPEDTMSVA
RFQQLARETIADLQSRGIRPILVGGSGLYARAAIDDITFPGTDPDVRTRLEEREKTEGAGALFDELRAKDPEAAARMDPR
NPRRTIRALEVIELTGKPYSASLPRYRYVIPSVQIGLDLDRPDLDHRIDLRTKQMYDDGFIEEVERLRPHLGATAVRALG
YQQIIDLLDGIWDVNDAFADIAQKTKRLARKQMGWFGRDPRIHWLQALNPKLVDNAMAIIAHADAGDYDSIDARADEYTQ
HHLGDITA

Sequences:

>Translated_328_residues
MTQRVVSIVGPTASGKTGLGIAIARRLAEAGERAEIVNADAYQMYRGMDIGTAKPTAEEQAVVSHHLIDIIDPEDTMSVA
RFQQLARETIADLQSRGIRPILVGGSGLYARAAIDDITFPGTDPDVRTRLEEREKTEGAGALFDELRAKDPEAAARMDPR
NPRRTIRALEVIELTGKPYSASLPRYRYVIPSVQIGLDLDRPDLDHRIDLRTKQMYDDGFIEEVERLRPHLGATAVRALG
YQQIIDLLDGIWDVNDAFADIAQKTKRLARKQMGWFGRDPRIHWLQALNPKLVDNAMAIIAHADAGDYDSIDARADEYTQ
HHLGDITA
>Mature_327_residues
TQRVVSIVGPTASGKTGLGIAIARRLAEAGERAEIVNADAYQMYRGMDIGTAKPTAEEQAVVSHHLIDIIDPEDTMSVAR
FQQLARETIADLQSRGIRPILVGGSGLYARAAIDDITFPGTDPDVRTRLEEREKTEGAGALFDELRAKDPEAAARMDPRN
PRRTIRALEVIELTGKPYSASLPRYRYVIPSVQIGLDLDRPDLDHRIDLRTKQMYDDGFIEEVERLRPHLGATAVRALGY
QQIIDLLDGIWDVNDAFADIAQKTKRLARKQMGWFGRDPRIHWLQALNPKLVDNAMAIIAHADAGDYDSIDARADEYTQH
HLGDITA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=30.4487179487179, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1790613, Length=315, Percent_Identity=34.2857142857143, Blast_Score=167, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI25144712, Length=270, Percent_Identity=25.9259259259259, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324848, Length=297, Percent_Identity=24.9158249158249, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24649830, Length=333, Percent_Identity=26.4264264264264, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36472; Mature: 36341

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRVVSIVGPTASGKTGLGIAIARRLAEAGERAEIVNADAYQMYRGMDIGTAKPTAEEQ
CCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCCCCCCCHHHH
AVVSHHLIDIIDPEDTMSVARFQQLARETIADLQSRGIRPILVGGSGLYARAAIDDITFP
HHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHHCCCCC
GTDPDVRTRLEEREKTEGAGALFDELRAKDPEAAARMDPRNPRRTIRALEVIELTGKPYS
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
ASLPRYRYVIPSVQIGLDLDRPDLDHRIDLRTKQMYDDGFIEEVERLRPHLGATAVRALG
CCCCCEEEECCCEEEECCCCCCCCCCEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YQQIIDLLDGIWDVNDAFADIAQKTKRLARKQMGWFGRDPRIHWLQALNPKLVDNAMAII
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHEEEE
AHADAGDYDSIDARADEYTQHHLGDITA
EECCCCCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQRVVSIVGPTASGKTGLGIAIARRLAEAGERAEIVNADAYQMYRGMDIGTAKPTAEEQ
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCCCCCCCHHHH
AVVSHHLIDIIDPEDTMSVARFQQLARETIADLQSRGIRPILVGGSGLYARAAIDDITFP
HHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHHCCCCC
GTDPDVRTRLEEREKTEGAGALFDELRAKDPEAAARMDPRNPRRTIRALEVIELTGKPYS
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
ASLPRYRYVIPSVQIGLDLDRPDLDHRIDLRTKQMYDDGFIEEVERLRPHLGATAVRALG
CCCCCEEEECCCEEEECCCCCCCCCCEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YQQIIDLLDGIWDVNDAFADIAQKTKRLARKQMGWFGRDPRIHWLQALNPKLVDNAMAII
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHEEEE
AHADAGDYDSIDARADEYTQHHLGDITA
EECCCCCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA