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The map label for this gene is ispG [H]

Identifier: 213691675

GI number: 213691675

Start: 903966

End: 905189

Strand: Direct

Name: ispG [H]

Synonym: Blon_0780

Alternate gene names: 213691675

Gene position: 903966-905189 (Clockwise)

Preceding gene: 213691674

Following gene: 213691676

Centisome position: 31.91

GC content: 63.56

Gene sequence:

>1224_bases
ATGATGAATACCTTGAATACGGAAAATCCCATCGAAAAGCCGTTCCGCAAAACCGGTGATCCGGTCGATCTGACCAGCGA
ATCGCCCTTGCACCCGCGCCGCAAGTCCCGTCGCATCATGGTGGGTCCGGTGCCGGTCGGCGGTGGCGCCCCCATCTCCG
TGCAGTCGATGACCAACACGCTCACGGCCGACGTGCCTGCCACGTTGCAGCAGATCGCCGAACTGACCGCCGCCGGCTGC
GACATCGTGCGCGTGGCCGTGCCGAGTCAGGACGATGCCGACGCGCTGCCGGAAATCTGCCGCAAATCGCCGATTCCGGT
CATCGCCGATATCCACTTCCAGTCCAAATACGTGTTCCAGGCCATCGACGCCGGCTGCGCGGCCGTGCGCGTGAACCCCG
GCAACATCCGTAAGTTCGACGAAGTCGGCCCGGACATCTGCAAGGCCGCCACAGACGCGGGCATCTCGCTGCGTATCGGC
GTGAACGCCGGCTCGCTGGACAAGGACCTGTACGCCAAATACGGTGGCCCGACTCCGGAAGCGCTGGTGGCCTCCGCCTT
GAAGGAAGCGCACATGTTCGAGGACGTCGGCTTCCACGATTTCAAGATCTCCGTCAAACACCATGACGTCATCACCATGG
TCGAAACGTACCGATTGCTCGCATCCAAGGGCGACTGGCCGCTGCACCTCGGCGTCACCGAAGCCGGCCCCGCCTGGCAA
GGCACCATCAAATCCTGCCTGGCGTTCGGCGCGCTCTTGGCCGAGGGCATTGGCGACACGATTCGCGTGTCCCTCTCCGC
GCCGCCGGTCGAAGAGGTCAAGGTGGGTTGCAAACTCTTGGAATACATGGGATTGCGCCCCCGCAAGTTCGACATCATCT
CCTGCCCGAGCTGCGGCCGCGCCCAAGTGGACGTGATCCAGCTCGCCTCCGCCGTCACCGAAGGCCTGAAGGACGTGACC
GCGCCGATCCGAGTGGCTGTTATGGGCTGCATCGTCAACGGTCCGGGCGAGGCGCGCGAAGCCGACCTCGGCGTGGCCTC
CGGCAACGGCAAAGGGCAGATCTTCATCAAAGGCAAGGTCATCAAGACCGTGCCCGAGGATCAGATCGTCGACACGCTGC
TCACCATAGCGAACGATATCGCCGCCCAGATGGAGGCCGACGGCCAAGTGCCAGTCAATTCCACCGGCCCGGTAGTGGTT
CCAATCCAGCATCCCGGTCACTGA

Upstream 100 bases:

>100_bases
ACGCCGAGCTTCGTGGTAACCCGCTGTTCACGGACGTGGAGGAAATGAACCAACTTGAGCTTGAAGCCCGCCGACGCGCC
GACGACCTGATAAACAAGCA

Downstream 100 bases:

>100_bases
TTCGGCCGAATAGGCCCTCGGCATAAATATCGCGGTCAGATTACAATAGCGCACGTGTCGCAAGAAGAAGAGCATCGCCG
TACTGTTCGATCCAAGCCGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MMNTLNTENPIEKPFRKTGDPVDLTSESPLHPRRKSRRIMVGPVPVGGGAPISVQSMTNTLTADVPATLQQIAELTAAGC
DIVRVAVPSQDDADALPEICRKSPIPVIADIHFQSKYVFQAIDAGCAAVRVNPGNIRKFDEVGPDICKAATDAGISLRIG
VNAGSLDKDLYAKYGGPTPEALVASALKEAHMFEDVGFHDFKISVKHHDVITMVETYRLLASKGDWPLHLGVTEAGPAWQ
GTIKSCLAFGALLAEGIGDTIRVSLSAPPVEEVKVGCKLLEYMGLRPRKFDIISCPSCGRAQVDVIQLASAVTEGLKDVT
APIRVAVMGCIVNGPGEAREADLGVASGNGKGQIFIKGKVIKTVPEDQIVDTLLTIANDIAAQMEADGQVPVNSTGPVVV
PIQHPGH

Sequences:

>Translated_407_residues
MMNTLNTENPIEKPFRKTGDPVDLTSESPLHPRRKSRRIMVGPVPVGGGAPISVQSMTNTLTADVPATLQQIAELTAAGC
DIVRVAVPSQDDADALPEICRKSPIPVIADIHFQSKYVFQAIDAGCAAVRVNPGNIRKFDEVGPDICKAATDAGISLRIG
VNAGSLDKDLYAKYGGPTPEALVASALKEAHMFEDVGFHDFKISVKHHDVITMVETYRLLASKGDWPLHLGVTEAGPAWQ
GTIKSCLAFGALLAEGIGDTIRVSLSAPPVEEVKVGCKLLEYMGLRPRKFDIISCPSCGRAQVDVIQLASAVTEGLKDVT
APIRVAVMGCIVNGPGEAREADLGVASGNGKGQIFIKGKVIKTVPEDQIVDTLLTIANDIAAQMEADGQVPVNSTGPVVV
PIQHPGH
>Mature_407_residues
MMNTLNTENPIEKPFRKTGDPVDLTSESPLHPRRKSRRIMVGPVPVGGGAPISVQSMTNTLTADVPATLQQIAELTAAGC
DIVRVAVPSQDDADALPEICRKSPIPVIADIHFQSKYVFQAIDAGCAAVRVNPGNIRKFDEVGPDICKAATDAGISLRIG
VNAGSLDKDLYAKYGGPTPEALVASALKEAHMFEDVGFHDFKISVKHHDVITMVETYRLLASKGDWPLHLGVTEAGPAWQ
GTIKSCLAFGALLAEGIGDTIRVSLSAPPVEEVKVGCKLLEYMGLRPRKFDIISCPSCGRAQVDVIQLASAVTEGLKDVT
APIRVAVMGCIVNGPGEAREADLGVASGNGKGQIFIKGKVIKTVPEDQIVDTLLTIANDIAAQMEADGQVPVNSTGPVVV
PIQHPGH

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=369, Percent_Identity=45.5284552845528, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 43039; Mature: 43039

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNTLNTENPIEKPFRKTGDPVDLTSESPLHPRRKSRRIMVGPVPVGGGAPISVQSMTNT
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCEEHHHHHHH
LTADVPATLQQIAELTAAGCDIVRVAVPSQDDADALPEICRKSPIPVIADIHFQSKYVFQ
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHH
AIDAGCAAVRVNPGNIRKFDEVGPDICKAATDAGISLRIGVNAGSLDKDLYAKYGGPTPE
HHCCCCEEEEECCCCCCCHHHCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCHH
ALVASALKEAHMFEDVGFHDFKISVKHHDVITMVETYRLLASKGDWPLHLGVTEAGPAWQ
HHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHH
GTIKSCLAFGALLAEGIGDTIRVSLSAPPVEEVKVGCKLLEYMGLRPRKFDIISCPSCGR
HHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
AQVDVIQLASAVTEGLKDVTAPIRVAVMGCIVNGPGEAREADLGVASGNGKGQIFIKGKV
CHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECEE
IKTVPEDQIVDTLLTIANDIAAQMEADGQVPVNSTGPVVVPIQHPGH
EECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MMNTLNTENPIEKPFRKTGDPVDLTSESPLHPRRKSRRIMVGPVPVGGGAPISVQSMTNT
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCCEEHHHHHHH
LTADVPATLQQIAELTAAGCDIVRVAVPSQDDADALPEICRKSPIPVIADIHFQSKYVFQ
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHH
AIDAGCAAVRVNPGNIRKFDEVGPDICKAATDAGISLRIGVNAGSLDKDLYAKYGGPTPE
HHCCCCEEEEECCCCCCCHHHCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCHH
ALVASALKEAHMFEDVGFHDFKISVKHHDVITMVETYRLLASKGDWPLHLGVTEAGPAWQ
HHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHH
GTIKSCLAFGALLAEGIGDTIRVSLSAPPVEEVKVGCKLLEYMGLRPRKFDIISCPSCGR
HHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
AQVDVIQLASAVTEGLKDVTAPIRVAVMGCIVNGPGEAREADLGVASGNGKGQIFIKGKV
CHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECEE
IKTVPEDQIVDTLLTIANDIAAQMEADGQVPVNSTGPVVVPIQHPGH
EECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]