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The map label for this gene is rspA [H]

Identifier: 213691565

GI number: 213691565

Start: 770590

End: 771837

Strand: Reverse

Name: rspA [H]

Synonym: Blon_0667

Alternate gene names: 213691565

Gene position: 771837-770590 (Counterclockwise)

Preceding gene: 213691566

Following gene: 213691564

Centisome position: 27.25

GC content: 59.86

Gene sequence:

>1248_bases
ATGGCAATCGAAAAAGCTGAAGTCTTCGTGACCAGCCCGGGACGTAATTTCGTCGTTTTCCGCATCACCACTACTGACGG
CGTCGTTGGACTTGGTGACGCCACGCTGAACGGACGTGAGCTCGTCGCCGCGAAGTACCTAGAGACCATCGCTCCGAGCC
TTATCGGCAAGAATGAGGACAACATCGAGGACATCTGGCAGTACCTGTACAAGGGTGCTTACTGGAAGCGTGGACCGGTG
ACCATGGCCGCCATCTCCGCTGTGGATGTGGCACTGTGGGACATCAAGGCCAAGAAGGCCGGCGTCCCGGTTTACGAGCT
GCTGGGCGGCGCTTCCCGCACCGGCATTCTGACCTACGCCCACGCTTCCGGTGTGGACTTCCCGAGCCTGTTCGATTCCA
TCGACATGTACCGCGACATGGGCTTCAAGGCCACCCGTATTCAGTTCGCTCTGCCGGGCATTCCGTCCACCTACGGTGTC
GCCGGTTCCGCTAACTCCCAGCAGGGCGCCGGCGAGAAGGGCCTGCGTTACGACTACGAGCCTGCCCGCCGTACCACGGT
GCCGGTCGAAGAGGTTTGGGACGCACGCACCTACCTCAACACGCTTCCGGGAGTGTTCGAGGAAGTTCGTAACAAGTACG
GCAAGGACCTCATCCTGCTGCACGATTCCCACCACCGTCTGAGCCCGATTCAGGCAGCTCGCTTCGGCAAGTCGCTCGAG
CCGTACGACCTGTTCTGGATGGAAGACTCCACTCCGGCCGAGGAGAATCAAGAATTCCTGCGCATCGTGCGTGAGCACAC
CACCACGCCTATCGCCATCGGCGAGATTATCAACTCGTGGAGCGATTACATCACCCTGATCAAGGAACAGCTCATCGACT
ACGTGCGTTCCGCCGTCACCCACACCGGTGGTCTGACCCACATGAAGAAGCTCATGGCCTTCGCCGAGCTCTTTGGTGTC
AAGTCTGGCTTCCACGGTCCGACCGACGTTTCCCCGGTCGGCATGGCTGCGAACCTGCACCTTGACCTAGCGATTCCGAA
CTTCGGCATCCAGGAGTACATGAAGCATTCCGAGGAGACTCTCGAGGTGTTCCACACTTCGTACACCTTCAAGGATGGAT
ATCTGCACCCGGGCAACAAGCCTGGGCTGGGCGTCGACTTCGACGAGAAGCTTGCGGCGAAGTATCCGTACCAGCCGGCT
CCGCTCCCCGTTGACCGCCTGCTTGACGGCACCATGCACGAGTGGTGA

Upstream 100 bases:

>100_bases
ACAACCATAAACATAATCAAATAACCGATAGCATAAGCTTCCTCGCCGTAGGTGGGGATTATGACGCGGACGCGCCGCGT
CATAACAGGAAGGAATAACC

Downstream 100 bases:

>100_bases
TTTAGCCACCGATTCATTATCGGGCGATTGGAACGTATTCGGGTCTGATCGCCCTTTCCATTCAAGGAGAATCCATGCTT
GATTCCAATGCCGAACCCGT

Product: Mandelate racemase/muconate lactonizing enzyme, N-terminal domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MAIEKAEVFVTSPGRNFVVFRITTTDGVVGLGDATLNGRELVAAKYLETIAPSLIGKNEDNIEDIWQYLYKGAYWKRGPV
TMAAISAVDVALWDIKAKKAGVPVYELLGGASRTGILTYAHASGVDFPSLFDSIDMYRDMGFKATRIQFALPGIPSTYGV
AGSANSQQGAGEKGLRYDYEPARRTTVPVEEVWDARTYLNTLPGVFEEVRNKYGKDLILLHDSHHRLSPIQAARFGKSLE
PYDLFWMEDSTPAEENQEFLRIVREHTTTPIAIGEIINSWSDYITLIKEQLIDYVRSAVTHTGGLTHMKKLMAFAELFGV
KSGFHGPTDVSPVGMAANLHLDLAIPNFGIQEYMKHSEETLEVFHTSYTFKDGYLHPGNKPGLGVDFDEKLAAKYPYQPA
PLPVDRLLDGTMHEW

Sequences:

>Translated_415_residues
MAIEKAEVFVTSPGRNFVVFRITTTDGVVGLGDATLNGRELVAAKYLETIAPSLIGKNEDNIEDIWQYLYKGAYWKRGPV
TMAAISAVDVALWDIKAKKAGVPVYELLGGASRTGILTYAHASGVDFPSLFDSIDMYRDMGFKATRIQFALPGIPSTYGV
AGSANSQQGAGEKGLRYDYEPARRTTVPVEEVWDARTYLNTLPGVFEEVRNKYGKDLILLHDSHHRLSPIQAARFGKSLE
PYDLFWMEDSTPAEENQEFLRIVREHTTTPIAIGEIINSWSDYITLIKEQLIDYVRSAVTHTGGLTHMKKLMAFAELFGV
KSGFHGPTDVSPVGMAANLHLDLAIPNFGIQEYMKHSEETLEVFHTSYTFKDGYLHPGNKPGLGVDFDEKLAAKYPYQPA
PLPVDRLLDGTMHEW
>Mature_414_residues
AIEKAEVFVTSPGRNFVVFRITTTDGVVGLGDATLNGRELVAAKYLETIAPSLIGKNEDNIEDIWQYLYKGAYWKRGPVT
MAAISAVDVALWDIKAKKAGVPVYELLGGASRTGILTYAHASGVDFPSLFDSIDMYRDMGFKATRIQFALPGIPSTYGVA
GSANSQQGAGEKGLRYDYEPARRTTVPVEEVWDARTYLNTLPGVFEEVRNKYGKDLILLHDSHHRLSPIQAARFGKSLEP
YDLFWMEDSTPAEENQEFLRIVREHTTTPIAIGEIINSWSDYITLIKEQLIDYVRSAVTHTGGLTHMKKLMAFAELFGVK
SGFHGPTDVSPVGMAANLHLDLAIPNFGIQEYMKHSEETLEVFHTSYTFKDGYLHPGNKPGLGVDFDEKLAAKYPYQPAP
LPVDRLLDGTMHEW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=416, Percent_Identity=57.9326923076923, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI48994953, Length=425, Percent_Identity=27.5294117647059, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 46160; Mature: 46029

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEKAEVFVTSPGRNFVVFRITTTDGVVGLGDATLNGRELVAAKYLETIAPSLIGKNED
CCCCCCEEEEECCCCEEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
NIEDIWQYLYKGAYWKRGPVTMAAISAVDVALWDIKAKKAGVPVYELLGGASRTGILTYA
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEHHCCCCCHHHHHCCCCCCCEEEEE
HASGVDFPSLFDSIDMYRDMGFKATRIQFALPGIPSTYGVAGSANSQQGAGEKGLRYDYE
CCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PARRTTVPVEEVWDARTYLNTLPGVFEEVRNKYGKDLILLHDSHHRLSPIQAARFGKSLE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCCCC
PYDLFWMEDSTPAEENQEFLRIVREHTTTPIAIGEIINSWSDYITLIKEQLIDYVRSAVT
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTGGLTHMKKLMAFAELFGVKSGFHGPTDVSPVGMAANLHLDLAIPNFGIQEYMKHSEET
HCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHH
LEVFHTSYTFKDGYLHPGNKPGLGVDFDEKLAAKYPYQPAPLPVDRLLDGTMHEW
HHHHHHEEEECCCEECCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
AIEKAEVFVTSPGRNFVVFRITTTDGVVGLGDATLNGRELVAAKYLETIAPSLIGKNED
CCCCCEEEEECCCCEEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
NIEDIWQYLYKGAYWKRGPVTMAAISAVDVALWDIKAKKAGVPVYELLGGASRTGILTYA
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEHHCCCCCHHHHHCCCCCCCEEEEE
HASGVDFPSLFDSIDMYRDMGFKATRIQFALPGIPSTYGVAGSANSQQGAGEKGLRYDYE
CCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PARRTTVPVEEVWDARTYLNTLPGVFEEVRNKYGKDLILLHDSHHRLSPIQAARFGKSLE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCCCC
PYDLFWMEDSTPAEENQEFLRIVREHTTTPIAIGEIINSWSDYITLIKEQLIDYVRSAVT
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTGGLTHMKKLMAFAELFGVKSGFHGPTDVSPVGMAANLHLDLAIPNFGIQEYMKHSEET
HCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHH
LEVFHTSYTFKDGYLHPGNKPGLGVDFDEKLAAKYPYQPAPLPVDRLLDGTMHEW
HHHHHHEEEECCCEECCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]