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The map label for this gene is regX3 [H]

Identifier: 213691278

GI number: 213691278

Start: 420759

End: 421529

Strand: Direct

Name: regX3 [H]

Synonym: Blon_0362

Alternate gene names: 213691278

Gene position: 420759-421529 (Clockwise)

Preceding gene: 213691277

Following gene: 213691279

Centisome position: 14.85

GC content: 61.48

Gene sequence:

>771_bases
ATGGTTACGATGGTGTCGGAAAATTTTGCGCCGATGCGCACGGGAACATATCCAAGAAAGGCTACTATGGCACGCATTCT
TATCGTCGAAGACGAGGAATCCTATCGCGAGCCGCTGGTGTATCAGCTCACGCGCGAGGGGTACGACGTGTCCGCCGCCG
CCACCGGCGAGGAAGGCCTGGAACTGTTCACCAAGGGCGGCATCGACTTGGTGCTGCTCGATCTGATGCTGCCGGGCATT
GACGGCACCGCGCTGTGCCGGCGCATCCGCGAGCAGAGCCGTGTGCCGATCATCATGCTCACCGCCAAGAGCGCCGAGAT
CGATAAGGTCGTCGGCCTTGAGATCGGCGCGGACGATTATGTGACCAAGCCGTACTCCTTCCGTGAGCTTCTGGCGCGCA
TCCGGGCCGTGATGCGCCGCAACCAGGCCACCGCCCAGGCCTCCGGCACCGTGGACGACGACATTCCGCTGGTGTGCGGC
GACATCGCCATGCAGGTGGGCCAGCATCAGGTCACCGTGCGCGGCGAGACCGTGTTCTTCCCGCTCAAGGAATTCGAGCT
GCTCGAATATCTCATGCAGAACAAGGGCCGCGTGATGACCCGTCATCAGTTGATCGACCGCATCTGGGGCTCGGATTACG
TGGGCGACACCAAGACGCTCGATGTGCATGTCAAGCGCGTGCGCTCCAAGATTGAGGAGGATCCGGCGCACCCCAAGTAT
CTGACTACCGTGCGCGGTCTCGGATACAAGATCGATACTCCCGCGGAGTAA

Upstream 100 bases:

>100_bases
CGGCGTCGGCCGATCGCATGGCGGGCTGGTTTCGCCCGTGGCGTGTAGCCTCATTGAGCAATTACGTACTTTCGGGAAAC
GGCAGGCTAACGGAGAACAA

Downstream 100 bases:

>100_bases
ATGGGAGGCGGCCTTCGGGTCGCCTTTTTTGTTGTGGATTGGGGGCTCGCCGCAGGTGAGTCCGCTCCAAAAACTACGTA
GCCTCAACGTAGCCCATAAA

Product: two component transcriptional regulator, winged helix family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MVTMVSENFAPMRTGTYPRKATMARILIVEDEESYREPLVYQLTREGYDVSAAATGEEGLELFTKGGIDLVLLDLMLPGI
DGTALCRRIREQSRVPIIMLTAKSAEIDKVVGLEIGADDYVTKPYSFRELLARIRAVMRRNQATAQASGTVDDDIPLVCG
DIAMQVGQHQVTVRGETVFFPLKEFELLEYLMQNKGRVMTRHQLIDRIWGSDYVGDTKTLDVHVKRVRSKIEEDPAHPKY
LTTVRGLGYKIDTPAE

Sequences:

>Translated_256_residues
MVTMVSENFAPMRTGTYPRKATMARILIVEDEESYREPLVYQLTREGYDVSAAATGEEGLELFTKGGIDLVLLDLMLPGI
DGTALCRRIREQSRVPIIMLTAKSAEIDKVVGLEIGADDYVTKPYSFRELLARIRAVMRRNQATAQASGTVDDDIPLVCG
DIAMQVGQHQVTVRGETVFFPLKEFELLEYLMQNKGRVMTRHQLIDRIWGSDYVGDTKTLDVHVKRVRSKIEEDPAHPKY
LTTVRGLGYKIDTPAE
>Mature_256_residues
MVTMVSENFAPMRTGTYPRKATMARILIVEDEESYREPLVYQLTREGYDVSAAATGEEGLELFTKGGIDLVLLDLMLPGI
DGTALCRRIREQSRVPIIMLTAKSAEIDKVVGLEIGADDYVTKPYSFRELLARIRAVMRRNQATAQASGTVDDDIPLVCG
DIAMQVGQHQVTVRGETVFFPLKEFELLEYLMQNKGRVMTRHQLIDRIWGSDYVGDTKTLDVHVKRVRSKIEEDPAHPKY
LTTVRGLGYKIDTPAE

Specific function: Part of the two-component regulatory system senX3/regX3. Once phosphorylated by senX3, activates the expression of several operons/genes involved in phosphate assimilation and metabolism, such as pstSCAB, phnDCE, and phoA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=226, Percent_Identity=41.5929203539823, Blast_Score=180, Evalue=7e-47,
Organism=Escherichia coli, GI1788394, Length=238, Percent_Identity=38.2352941176471, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=38.0952380952381, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI87082012, Length=230, Percent_Identity=36.0869565217391, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1786911, Length=230, Percent_Identity=38.2608695652174, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1786599, Length=232, Percent_Identity=37.0689655172414, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=34.2105263157895, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=34.3612334801762, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789402, Length=228, Percent_Identity=34.6491228070175, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1787229, Length=226, Percent_Identity=37.1681415929204, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1790552, Length=228, Percent_Identity=34.6491228070175, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=33.7719298245614, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1787375, Length=227, Percent_Identity=33.0396475770925, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=30.7359307359307, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788550, Length=170, Percent_Identity=31.1764705882353, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=35.9223300970874, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790437, Length=142, Percent_Identity=30.9859154929577, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28798; Mature: 28798

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTMVSENFAPMRTGTYPRKATMARILIVEDEESYREPLVYQLTREGYDVSAAATGEEGL
CEEEECCCCCCCCCCCCCCCCCEEEEEEEECCHHHHCCCEEEHHHCCCCEEEECCCHHHH
ELFTKGGIDLVLLDLMLPGIDGTALCRRIREQSRVPIIMLTAKSAEIDKVVGLEIGADDY
HHHHCCCHHEEEEEHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCC
VTKPYSFRELLARIRAVMRRNQATAQASGTVDDDIPLVCGDIAMQVGQHQVTVRGETVFF
CCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEECCEEEE
PLKEFELLEYLMQNKGRVMTRHQLIDRIWGSDYVGDTKTLDVHVKRVRSKIEEDPAHPKY
EHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHH
LTTVRGLGYKIDTPAE
HHHHHHCCEECCCCCC
>Mature Secondary Structure
MVTMVSENFAPMRTGTYPRKATMARILIVEDEESYREPLVYQLTREGYDVSAAATGEEGL
CEEEECCCCCCCCCCCCCCCCCEEEEEEEECCHHHHCCCEEEHHHCCCCEEEECCCHHHH
ELFTKGGIDLVLLDLMLPGIDGTALCRRIREQSRVPIIMLTAKSAEIDKVVGLEIGADDY
HHHHCCCHHEEEEEHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCC
VTKPYSFRELLARIRAVMRRNQATAQASGTVDDDIPLVCGDIAMQVGQHQVTVRGETVFF
CCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEECCEEEE
PLKEFELLEYLMQNKGRVMTRHQLIDRIWGSDYVGDTKTLDVHVKRVRSKIEEDPAHPKY
EHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHH
LTTVRGLGYKIDTPAE
HHHHHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA