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The map label for this gene is pstS2 [H]

Identifier: 213691279

GI number: 213691279

Start: 421767

End: 422900

Strand: Direct

Name: pstS2 [H]

Synonym: Blon_0363

Alternate gene names: 213691279

Gene position: 421767-422900 (Clockwise)

Preceding gene: 213691278

Following gene: 213691280

Centisome position: 14.89

GC content: 64.81

Gene sequence:

>1134_bases
ATGCAGAAGAACATCCTCGTTCGCTCCATTGCCGCGCTGTCCGGCATTGTGATGCTTGCTTCCGTGGCTGCCTGCGGCGA
CAACAGCGCCGCGAAGACCGACAACAACGCTTCCTCCGACTCCACCGCCAAGGCCGCTCCGGTCTCCGGCGACTTCCAGG
GCGCCGGCGCCTCCTCCCAGCAGGCCGCCGTCGAAGCTTGGATCGCCGGCTTCCAGGGCTCCAACCCGGACGCCAAGATC
GCCTACAACCCGTCCGGTTCCGGCGCCGGCGTGCAGACCTTCCTGACCGGCGCCACCGCATGGGCCGGCTCCGACAAGGC
CCTCGCCGATAACGAGGTCGAGCAGTCCAAGTCCGTGTGCGCCTCCGGCTCCGCCTTCGACGTTCCGGTCTACATCTCCC
CGATCGCCGTGGTCTTCAACCTCAAGGGCGTTTCCGATGCCGGCAAGCACATCAACATGGACGCCGCCACCATCGCCAAG
ATCTTCGACGGCAAGATCACCAAGTGGAACGACCCGGCCATCGCCGAGCAGAACAAGGACCTGAAGCTGCCCGACACCGA
CATCACCGTCGTGCACCGCTCCGATAAGTCCGGCACCACCCAGAACTTCGTCTCCTACTTCAAGGACGTCACCCCGGACA
ACTGGAACTACGACCTCTCCGAGAACTGGCCGAACGAGGTCGGCCAGGGCGCCAAGGGCACTTCCGGCGTGATCTCCACC
GTCAAGCAGGCCGACGGCACCATCGGCTACGCCGACTTCTCCCAGGTCGGCGATCTGGGCACCGTGGCCGTCAAGGTCGG
CGAGAAGTACAACGTGATCTCCGCCAAGGCCGGCTCCAAGGTCATCGAGGACTCCAAGCAGGACACCACCGTCACCGGTG
ACAACCGCGTCGTCATCAAGATCAACCACGCCACCAAGGCCGACGGCGCCTACCCGATCGTCCTGGTCTCCTACGATGTC
GTCTGCCCGGCCTACAAGGACGCCAAGCAGGCCGAGTTCGCCAAGGCATGGCTGACCTACGTGACCTCCGACGAAGGCCA
GAAGGCCGCTCAGGATGCCGCCGGCACCGCTCCGCTGCCCGCCTCCCTGAAGACCAAGGTCGCCAAGTCCATCGAGGCCA
TCAAGACCAAGTGA

Upstream 100 bases:

>100_bases
CCGCCTTTTCGAAACCGCTAATAGTCTTGTAGTTGTCAAGAATTGACCGGGCGGTTTCGCTCCGCCCGCGAATAGTTGAT
AGAGGGATTAGGTAATAATC

Downstream 100 bases:

>100_bases
TGCCGTCGGCCCACAGGGTCGCATGGACTGGGCCTGACCTCTGAACGCCGAACTGTGAACGCACAGTCGCCAGCATGAAG
CCGCAGTCGTAAACCATACG

Product: phosphate ABC transporter, periplasmic phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 2; PstS-2 [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MQKNILVRSIAALSGIVMLASVAACGDNSAAKTDNNASSDSTAKAAPVSGDFQGAGASSQQAAVEAWIAGFQGSNPDAKI
AYNPSGSGAGVQTFLTGATAWAGSDKALADNEVEQSKSVCASGSAFDVPVYISPIAVVFNLKGVSDAGKHINMDAATIAK
IFDGKITKWNDPAIAEQNKDLKLPDTDITVVHRSDKSGTTQNFVSYFKDVTPDNWNYDLSENWPNEVGQGAKGTSGVIST
VKQADGTIGYADFSQVGDLGTVAVKVGEKYNVISAKAGSKVIEDSKQDTTVTGDNRVVIKINHATKADGAYPIVLVSYDV
VCPAYKDAKQAEFAKAWLTYVTSDEGQKAAQDAAGTAPLPASLKTKVAKSIEAIKTK

Sequences:

>Translated_377_residues
MQKNILVRSIAALSGIVMLASVAACGDNSAAKTDNNASSDSTAKAAPVSGDFQGAGASSQQAAVEAWIAGFQGSNPDAKI
AYNPSGSGAGVQTFLTGATAWAGSDKALADNEVEQSKSVCASGSAFDVPVYISPIAVVFNLKGVSDAGKHINMDAATIAK
IFDGKITKWNDPAIAEQNKDLKLPDTDITVVHRSDKSGTTQNFVSYFKDVTPDNWNYDLSENWPNEVGQGAKGTSGVIST
VKQADGTIGYADFSQVGDLGTVAVKVGEKYNVISAKAGSKVIEDSKQDTTVTGDNRVVIKINHATKADGAYPIVLVSYDV
VCPAYKDAKQAEFAKAWLTYVTSDEGQKAAQDAAGTAPLPASLKTKVAKSIEAIKTK
>Mature_377_residues
MQKNILVRSIAALSGIVMLASVAACGDNSAAKTDNNASSDSTAKAAPVSGDFQGAGASSQQAAVEAWIAGFQGSNPDAKI
AYNPSGSGAGVQTFLTGATAWAGSDKALADNEVEQSKSVCASGSAFDVPVYISPIAVVFNLKGVSDAGKHINMDAATIAK
IFDGKITKWNDPAIAEQNKDLKLPDTDITVVHRSDKSGTTQNFVSYFKDVTPDNWNYDLSENWPNEVGQGAKGTSGVIST
VKQADGTIGYADFSQVGDLGTVAVKVGEKYNVISAKAGSKVIEDSKQDTTVTGDNRVVIKINHATKADGAYPIVLVSYDV
VCPAYKDAKQAEFAKAWLTYVTSDEGQKAAQDAAGTAPLPASLKTKVAKSIEAIKTK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=205, Percent_Identity=31.219512195122, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39186; Mature: 39186

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKNILVRSIAALSGIVMLASVAACGDNSAAKTDNNASSDSTAKAAPVSGDFQGAGASSQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
QAAVEAWIAGFQGSNPDAKIAYNPSGSGAGVQTFLTGATAWAGSDKALADNEVEQSKSVC
HHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHCCCEECCCCCCCCCCHHHHHHHHH
ASGSAFDVPVYISPIAVVFNLKGVSDAGKHINMDAATIAKIFDGKITKWNDPAIAEQNKD
CCCCCEECEEEECCEEEEEEECCCCCCCCEECCHHHHHHHHHCCCCCCCCCCCCCCCCCC
LKLPDTDITVVHRSDKSGTTQNFVSYFKDVTPDNWNYDLSENWPNEVGQGAKGTSGVIST
CCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHH
VKQADGTIGYADFSQVGDLGTVAVKVGEKYNVISAKAGSKVIEDSKQDTTVTGDNRVVIK
HHHCCCCCCCCCHHHCCCCEEEEEEECCCEEEEECCCCCHHHHCCCCCCEEECCCEEEEE
INHATKADGAYPIVLVSYDVVCPAYKDAKQAEFAKAWLTYVTSDEGQKAAQDAAGTAPLP
ECCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHEECCCCHHHHHHCCCCCCCC
ASLKTKVAKSIEAIKTK
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQKNILVRSIAALSGIVMLASVAACGDNSAAKTDNNASSDSTAKAAPVSGDFQGAGASSQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
QAAVEAWIAGFQGSNPDAKIAYNPSGSGAGVQTFLTGATAWAGSDKALADNEVEQSKSVC
HHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHCCCEECCCCCCCCCCHHHHHHHHH
ASGSAFDVPVYISPIAVVFNLKGVSDAGKHINMDAATIAKIFDGKITKWNDPAIAEQNKD
CCCCCEECEEEECCEEEEEEECCCCCCCCEECCHHHHHHHHHCCCCCCCCCCCCCCCCCC
LKLPDTDITVVHRSDKSGTTQNFVSYFKDVTPDNWNYDLSENWPNEVGQGAKGTSGVIST
CCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHH
VKQADGTIGYADFSQVGDLGTVAVKVGEKYNVISAKAGSKVIEDSKQDTTVTGDNRVVIK
HHHCCCCCCCCCHHHCCCCEEEEEEECCCEEEEECCCCCHHHHCCCCCCEEECCCEEEEE
INHATKADGAYPIVLVSYDVVCPAYKDAKQAEFAKAWLTYVTSDEGQKAAQDAAGTAPLP
ECCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHEECCCCHHHHHHCCCCCCCC
ASLKTKVAKSIEAIKTK
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9139906; 9634230; 12218036 [H]