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The map label for this gene is lacZ [H]

Identifier: 213691188

GI number: 213691188

Start: 317572

End: 319392

Strand: Reverse

Name: lacZ [H]

Synonym: Blon_0268

Alternate gene names: 213691188

Gene position: 319392-317572 (Counterclockwise)

Preceding gene: 213691189

Following gene: 213691187

Centisome position: 11.27

GC content: 62.82

Gene sequence:

>1821_bases
ATGGAGCGAATCCAATACCCCGACCCCCAATGCGTCCGACGCAACTGGGTCGATCTCAACGGCACCTGGGACTTCGCGAT
CCCCGGCGCACCGGCCGGGACCAAGAACGGCAACCAAGGCAACAACGCGATGGAGTCCACACCCGAGACCCTCCAAGCCG
CGGCCTCGATGGCCTTCCCCGAATTCGACCGCACCATCGAGGTGCCCTACAGCTACACCTTCCCCAAGTCCGGCGTGGAC
GAACCGCAGTATCACCCCGTCGTGTGGTACCGCCGCTCCTTCGACCTCGACATGCGCCCGGGCAAACGATACCTGCTCAA
CTTCGAGGCCGTCGACTATGCCTGCGACGTGTGGCTCAACGGCCGACACGTCGGCTCGCACCAAGGCGGACACACGCCCT
TCCGCTTCGATGTGACGCCTTACGCGGCCAAGGAAAACGAACTGGTCGTCAAGGTCGTCGATTTCAACCGCACCGACCAA
CCGCTCGGCAAGCAGGCATGGAAGGACGGCAACTTCGCCTGCTGGTACACGCGCACCATCGGCATCTGGCAGAGCGTGTG
GCTCGAAGAGACCGGCGACGTGCATCTGACCGACTTCACGATGACCCCGCACGCCAACCAAGCGCGCCTCGACGTGGATG
CCTCGCTCAATGTCAACGTCGACGCGGAGCTCGACTACAACGTGACCTTCGAAGGCAAGCCGGTCACGCACGGCACCGTC
TCCTTCACCAACGCGCGCGCCCGGTTCTCCGTCGAGATCCGCAACTACGACCCGTCCCCCGAACCGCATCCATGGACGCC
CTCGACGCCGGACCTCTACGACATCGACTTCACCGTGCGCTCCGGCGGCGAGACCACCGACGAGGTGTCCAGCTACTTCG
GTCTGCGTGATGTAACCAGCGACGGGAAGATGATCTTCCTCAACGACCAGAAGTACTACATGAAGCTGCTGCTCAACCAG
GGCTATTACCCGGGCGGAGGATTGACGGGCACCGCCGACGAGTTCCGCGCCGACATCGAGAAGATGAAGGCCATGGGCTT
CAACGGCAACCGCATCCATCAGAAGCTCGAGTCCAACCGCATGCTCTACCTGTGCGACAAGCTCGGTTTCATCGTCTGGG
CCGAGATGCCCAGCGCCTACGAATTCGGCCCGGAGCTCATCGCCAATGTGGAGCGCGAGCTGCCCGCCTACCTGCGCAAG
CACGTCAACCACCCCTCGGTGTTCGCCTATGTGCTGATGAACGAGTCGTGGGGCGTCTTCGACATCGCCCACAGCGAGCA
GGAGCGCACCTTCGTCAACTCGCTCTATTATCAGGCGAAGGCCTTCGATCCCTCGCGCCTGGTCATCGGCAACGATGGCT
ACGAGCAGACGCGCACCGACATCGCCACGATCCACGACTACAACGCCGATGCCGACGAACTGCTGGAGACCTATCAGGGC
CGTCGCGACGAAGTGTTGGCTGGAGCACCCTCCCCGATGGGCGGCCGCCGCGTGTTCTGCCAGGGGTACGAACAGCAGCG
CGTGCCGGTGATGGTCACCGAATACGGCGGCGTGGCCTATGAGCCGGGCGGTCACACCGAGGATTCGTGGGGCTACGGTG
AGCGTATCACCGACCCCGACAAGGTCGTTGATCAGATCGCGGCCCTGACGCGGGCCGTGATGGACATCGACTATTGCGTT
GGCTTCTGCTACACGCAGACCTCCGACGTGGAACAGGAGGTCAACGGCCTGCTCGACCACGACCACGAATACAAATTCGA
CCCCAAGCGCATACGAGAGGTGTTGCTGAGCAAGCACACCTACGGCTACGTGCAGGTGTGA

Upstream 100 bases:

>100_bases
CAACAAGGAAGTGACTGCGACAAAGTAGTGCGATAACAGTACTTTCAATCACAAAGACAACGACATTGTTTTTGTGAAAC
GATACAGAGAAAGGAAAGCA

Downstream 100 bases:

>100_bases
GAACGAGCGCCGACATGGGTGCGGATATGGCCACCCTTCACAATCGAACCAATAAATAGAGAAAGGGAACAATATGACAT
CGGCAACGGCAGCCGCACCG

Product: glycoside hydrolase family 2, sugar binding

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MERIQYPDPQCVRRNWVDLNGTWDFAIPGAPAGTKNGNQGNNAMESTPETLQAAASMAFPEFDRTIEVPYSYTFPKSGVD
EPQYHPVVWYRRSFDLDMRPGKRYLLNFEAVDYACDVWLNGRHVGSHQGGHTPFRFDVTPYAAKENELVVKVVDFNRTDQ
PLGKQAWKDGNFACWYTRTIGIWQSVWLEETGDVHLTDFTMTPHANQARLDVDASLNVNVDAELDYNVTFEGKPVTHGTV
SFTNARARFSVEIRNYDPSPEPHPWTPSTPDLYDIDFTVRSGGETTDEVSSYFGLRDVTSDGKMIFLNDQKYYMKLLLNQ
GYYPGGGLTGTADEFRADIEKMKAMGFNGNRIHQKLESNRMLYLCDKLGFIVWAEMPSAYEFGPELIANVERELPAYLRK
HVNHPSVFAYVLMNESWGVFDIAHSEQERTFVNSLYYQAKAFDPSRLVIGNDGYEQTRTDIATIHDYNADADELLETYQG
RRDEVLAGAPSPMGGRRVFCQGYEQQRVPVMVTEYGGVAYEPGGHTEDSWGYGERITDPDKVVDQIAALTRAVMDIDYCV
GFCYTQTSDVEQEVNGLLDHDHEYKFDPKRIREVLLSKHTYGYVQV

Sequences:

>Translated_606_residues
MERIQYPDPQCVRRNWVDLNGTWDFAIPGAPAGTKNGNQGNNAMESTPETLQAAASMAFPEFDRTIEVPYSYTFPKSGVD
EPQYHPVVWYRRSFDLDMRPGKRYLLNFEAVDYACDVWLNGRHVGSHQGGHTPFRFDVTPYAAKENELVVKVVDFNRTDQ
PLGKQAWKDGNFACWYTRTIGIWQSVWLEETGDVHLTDFTMTPHANQARLDVDASLNVNVDAELDYNVTFEGKPVTHGTV
SFTNARARFSVEIRNYDPSPEPHPWTPSTPDLYDIDFTVRSGGETTDEVSSYFGLRDVTSDGKMIFLNDQKYYMKLLLNQ
GYYPGGGLTGTADEFRADIEKMKAMGFNGNRIHQKLESNRMLYLCDKLGFIVWAEMPSAYEFGPELIANVERELPAYLRK
HVNHPSVFAYVLMNESWGVFDIAHSEQERTFVNSLYYQAKAFDPSRLVIGNDGYEQTRTDIATIHDYNADADELLETYQG
RRDEVLAGAPSPMGGRRVFCQGYEQQRVPVMVTEYGGVAYEPGGHTEDSWGYGERITDPDKVVDQIAALTRAVMDIDYCV
GFCYTQTSDVEQEVNGLLDHDHEYKFDPKRIREVLLSKHTYGYVQV
>Mature_606_residues
MERIQYPDPQCVRRNWVDLNGTWDFAIPGAPAGTKNGNQGNNAMESTPETLQAAASMAFPEFDRTIEVPYSYTFPKSGVD
EPQYHPVVWYRRSFDLDMRPGKRYLLNFEAVDYACDVWLNGRHVGSHQGGHTPFRFDVTPYAAKENELVVKVVDFNRTDQ
PLGKQAWKDGNFACWYTRTIGIWQSVWLEETGDVHLTDFTMTPHANQARLDVDASLNVNVDAELDYNVTFEGKPVTHGTV
SFTNARARFSVEIRNYDPSPEPHPWTPSTPDLYDIDFTVRSGGETTDEVSSYFGLRDVTSDGKMIFLNDQKYYMKLLLNQ
GYYPGGGLTGTADEFRADIEKMKAMGFNGNRIHQKLESNRMLYLCDKLGFIVWAEMPSAYEFGPELIANVERELPAYLRK
HVNHPSVFAYVLMNESWGVFDIAHSEQERTFVNSLYYQAKAFDPSRLVIGNDGYEQTRTDIATIHDYNADADELLETYQG
RRDEVLAGAPSPMGGRRVFCQGYEQQRVPVMVTEYGGVAYEPGGHTEDSWGYGERITDPDKVVDQIAALTRAVMDIDYCV
GFCYTQTSDVEQEVNGLLDHDHEYKFDPKRIREVLLSKHTYGYVQV

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI48994920, Length=486, Percent_Identity=23.2510288065844, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787903, Length=424, Percent_Identity=25.7075471698113, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17510825, Length=416, Percent_Identity=25.2403846153846, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 69008; Mature: 69008

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIQYPDPQCVRRNWVDLNGTWDFAIPGAPAGTKNGNQGNNAMESTPETLQAAASMAFP
CCCCCCCCHHHHHCCCEECCCEEEEECCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCC
EFDRTIEVPYSYTFPKSGVDEPQYHPVVWYRRSFDLDMRPGKRYLLNFEAVDYACDVWLN
CCCCEEECCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEECEEEEEEEC
GRHVGSHQGGHTPFRFDVTPYAAKENELVVKVVDFNRTDQPLGKQAWKDGNFACWYTRTI
CEEECCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCEEEEEEEEH
GIWQSVWLEETGDVHLTDFTMTPHANQARLDVDASLNVNVDAELDYNVTFEGKPVTHGTV
HHHHHHHHCCCCCEEEEEEEECCCCCCEEEEECCEEEEEEECEEEEEEEECCCCCCCCEE
SFTNARARFSVEIRNYDPSPEPHPWTPSTPDLYDIDFTVRSGGETTDEVSSYFGLRDVTS
EEECCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHCCCEEECC
DGKMIFLNDQKYYMKLLLNQGYYPGGGLTGTADEFRADIEKMKAMGFNGNRIHQKLESNR
CCCEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
MLYLCDKLGFIVWAEMPSAYEFGPELIANVERELPAYLRKHVNHPSVFAYVLMNESWGVF
EEEEECCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
DIAHSEQERTFVNSLYYQAKAFDPSRLVIGNDGYEQTRTDIATIHDYNADADELLETYQG
EECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCEEEEECCCCCHHHHHHHHCC
RRDEVLAGAPSPMGGRRVFCQGYEQQRVPVMVTEYGGVAYEPGGHTEDSWGYGERITDPD
CCCCEEECCCCCCCCCEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCHH
KVVDQIAALTRAVMDIDYCVGFCYTQTSDVEQEVNGLLDHDHEYKFDPKRIREVLLSKHT
HHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
YGYVQV
CEEEEC
>Mature Secondary Structure
MERIQYPDPQCVRRNWVDLNGTWDFAIPGAPAGTKNGNQGNNAMESTPETLQAAASMAFP
CCCCCCCCHHHHHCCCEECCCEEEEECCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCC
EFDRTIEVPYSYTFPKSGVDEPQYHPVVWYRRSFDLDMRPGKRYLLNFEAVDYACDVWLN
CCCCEEECCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEECEEEEEEEC
GRHVGSHQGGHTPFRFDVTPYAAKENELVVKVVDFNRTDQPLGKQAWKDGNFACWYTRTI
CEEECCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCEEEEEEEEH
GIWQSVWLEETGDVHLTDFTMTPHANQARLDVDASLNVNVDAELDYNVTFEGKPVTHGTV
HHHHHHHHCCCCCEEEEEEEECCCCCCEEEEECCEEEEEEECEEEEEEEECCCCCCCCEE
SFTNARARFSVEIRNYDPSPEPHPWTPSTPDLYDIDFTVRSGGETTDEVSSYFGLRDVTS
EEECCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHCCCEEECC
DGKMIFLNDQKYYMKLLLNQGYYPGGGLTGTADEFRADIEKMKAMGFNGNRIHQKLESNR
CCCEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
MLYLCDKLGFIVWAEMPSAYEFGPELIANVERELPAYLRKHVNHPSVFAYVLMNESWGVF
EEEEECCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
DIAHSEQERTFVNSLYYQAKAFDPSRLVIGNDGYEQTRTDIATIHDYNADADELLETYQG
EECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCEEEEECCCCCHHHHHHHHCC
RRDEVLAGAPSPMGGRRVFCQGYEQQRVPVMVTEYGGVAYEPGGHTEDSWGYGERITDPD
CCCCEEECCCCCCCCCEEEECCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCHH
KVVDQIAALTRAVMDIDYCVGFCYTQTSDVEQEVNGLLDHDHEYKFDPKRIREVLLSKHT
HHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
YGYVQV
CEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA