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The map label for this gene is ynaJ [H]

Identifier: 213691187

GI number: 213691187

Start: 316053

End: 317498

Strand: Reverse

Name: ynaJ [H]

Synonym: Blon_0267

Alternate gene names: 213691187

Gene position: 317498-316053 (Counterclockwise)

Preceding gene: 213691188

Following gene: 213691186

Centisome position: 11.21

GC content: 60.24

Gene sequence:

>1446_bases
ATGACATCGGCAACGGCAGCCGCACCGGTCAAATCCAGCAATGCGGACAAAGTGAGCCTCAAGGAATACGTGGCGTATTT
CTGCTACGGATTCGGACAGTGCTTCAGCTACGGCATCATCGGCAGCTTCATCCTGATCTACTACACGGATGTGGTCGGCA
TCTCCTCGGTGGTCGCCAGCGCGATCTTCCTGGTCGCCCGCCTGTGGGACGCCGTCTTCGACCCGCTGGTCTCCGGGTTC
ATGGACTCCCGCAAGCCCAGCAAGCAAGGCAAGTTCCGCAAGTGGATGAAGATCGCCCCGATCTTCGTGGTCGTCGCGAC
GATCGCCTGCTTCATCGACCCGGACATCCCGGTGGCCGGCAAGATCGTCTTCTGCGGCACCACCTACATCCTGTGGGGCA
CCTGCTACGCCTTCTCCGACATCCCGTTCTGGTCGCTGTCCACCGTGATGTCGCATGACCGCCAGACCCGCACCGACCTA
GTGACCGTCGCCAACATCGGCGTGTCCGCCGGCATCGCCATGGCCGGCATCATCATGCCGGTGATGCTCGCGCAGTTCAA
GCAGAACAACACTCCGTCGATGAGCTATCTGATCTCCGTGTCGATCCTCATGATCGCGGGCTTCGTGATCATGGAGTTCG
GCTACCTGTTCACCAAGGAGCGCATCGCCGCCTCCGACAAGGCGGAGAAGCTCTCCTACAAGCAGGTGTTCGAGGCCGTC
AAGGCGAACAAGTACATGATGATGATCCTGGTGATCTTCTTCCTGAAGTTCTTCATGGAAATGGTCAACAACATGATGAT
CTACTTCTTCACCTACAACATGTCGAACGCCGGGCTGATGAGTGGCTTCGGCCTGATCGGCACCTTCAGCGCGCTGACCT
TCCTGCTCATCCCGGTGCTGACCCGCCGCTACAAGAAGGTGAACCTCGTCCGCGTGCTGCTCATCGCCGAGATCGCCTTC
CGCGTGGTCTTCTACTTCGTCGGATACTCGAACACGACATTGACCTTCGTGTTCATCGGCATCACCTGGTGCATGTACTC
GATGAGCAACCCGATCCTGTCCGCCATGCTGGCCGAGACTATCGAATACAGCCAGGTCAAGACCGGGCACCGTGCGGAGG
CCGTGGTCTTCGGCGGCCAGACCTTCTCCAGCAAGCTGGCCATCGCCCTCGCCGGCGCCACGACCGGTCTGACGCTCACT
TTCCTCGGCTATCAGCCGAACGTCGCGCAGACGTCGGGCACGCTGAACGGCCTGTTCATCGTGATCTCGATCCTGCCGGC
GCTGGGCGCGCTGCTCAAGCTGCTGATTCTGACGAAGTACGACTACACCGAGACCGAGTTCAAGGAATACCAGCGCATCC
TGGTCGAACGCGGCGCGGAGGGATACCAGACCGCACCCGCCGATCTGGTGTCCGAGACCGCCGCGTCTGGCTCGGCGACA
AAGTAA

Upstream 100 bases:

>100_bases
CACACCTACGGCTACGTGCAGGTGTGAGAACGAGCGCCGACATGGGTGCGGATATGGCCACCCTTCACAATCGAACCAAT
AAATAGAGAAAGGGAACAAT

Downstream 100 bases:

>100_bases
GGATATCCGCTCCACACAATGACTGAGAGGGCTCCGTGTCCGATGCGTTCCGGCATCGGACACGGAGCCCTTCGACGTTT
CGGGAGAACGGGACGGCCGT

Product: sugar (glycoside-Pentoside-Hexuronide) transporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTSATAAAPVKSSNADKVSLKEYVAYFCYGFGQCFSYGIIGSFILIYYTDVVGISSVVASAIFLVARLWDAVFDPLVSGF
MDSRKPSKQGKFRKWMKIAPIFVVVATIACFIDPDIPVAGKIVFCGTTYILWGTCYAFSDIPFWSLSTVMSHDRQTRTDL
VTVANIGVSAGIAMAGIIMPVMLAQFKQNNTPSMSYLISVSILMIAGFVIMEFGYLFTKERIAASDKAEKLSYKQVFEAV
KANKYMMMILVIFFLKFFMEMVNNMMIYFFTYNMSNAGLMSGFGLIGTFSALTFLLIPVLTRRYKKVNLVRVLLIAEIAF
RVVFYFVGYSNTTLTFVFIGITWCMYSMSNPILSAMLAETIEYSQVKTGHRAEAVVFGGQTFSSKLAIALAGATTGLTLT
FLGYQPNVAQTSGTLNGLFIVISILPALGALLKLLILTKYDYTETEFKEYQRILVERGAEGYQTAPADLVSETAASGSAT
K

Sequences:

>Translated_481_residues
MTSATAAAPVKSSNADKVSLKEYVAYFCYGFGQCFSYGIIGSFILIYYTDVVGISSVVASAIFLVARLWDAVFDPLVSGF
MDSRKPSKQGKFRKWMKIAPIFVVVATIACFIDPDIPVAGKIVFCGTTYILWGTCYAFSDIPFWSLSTVMSHDRQTRTDL
VTVANIGVSAGIAMAGIIMPVMLAQFKQNNTPSMSYLISVSILMIAGFVIMEFGYLFTKERIAASDKAEKLSYKQVFEAV
KANKYMMMILVIFFLKFFMEMVNNMMIYFFTYNMSNAGLMSGFGLIGTFSALTFLLIPVLTRRYKKVNLVRVLLIAEIAF
RVVFYFVGYSNTTLTFVFIGITWCMYSMSNPILSAMLAETIEYSQVKTGHRAEAVVFGGQTFSSKLAIALAGATTGLTLT
FLGYQPNVAQTSGTLNGLFIVISILPALGALLKLLILTKYDYTETEFKEYQRILVERGAEGYQTAPADLVSETAASGSAT
K
>Mature_480_residues
TSATAAAPVKSSNADKVSLKEYVAYFCYGFGQCFSYGIIGSFILIYYTDVVGISSVVASAIFLVARLWDAVFDPLVSGFM
DSRKPSKQGKFRKWMKIAPIFVVVATIACFIDPDIPVAGKIVFCGTTYILWGTCYAFSDIPFWSLSTVMSHDRQTRTDLV
TVANIGVSAGIAMAGIIMPVMLAQFKQNNTPSMSYLISVSILMIAGFVIMEFGYLFTKERIAASDKAEKLSYKQVFEAVK
ANKYMMMILVIFFLKFFMEMVNNMMIYFFTYNMSNAGLMSGFGLIGTFSALTFLLIPVLTRRYKKVNLVRVLLIAEIAFR
VVFYFVGYSNTTLTFVFIGITWCMYSMSNPILSAMLAETIEYSQVKTGHRAEAVVFGGQTFSSKLAIALAGATTGLTLTF
LGYQPNVAQTSGTLNGLFIVISILPALGALLKLLILTKYDYTETEFKEYQRILVERGAEGYQTAPADLVSETAASGSATK

Specific function: Responsible For Melibiose Transport. It Is Capable Of Using Hydrogen, Sodium, And Lithium Cations As Coupling Cations For Cotransport, Depending On The Particular Sugar Transported (Symport System). [C]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=464, Percent_Identity=25.4310344827586, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082306, Length=470, Percent_Identity=24.2553191489362, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786466, Length=438, Percent_Identity=23.972602739726, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI145693206, Length=446, Percent_Identity=26.9058295964126, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994989, Length=450, Percent_Identity=25.3333333333333, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1787902, Length=456, Percent_Identity=24.5614035087719, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53011; Mature: 52880

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure 
TSATAAAPVKSSNADKVSLKEYVAYFCYGFGQCFSYGIIGSFILIYYTDVVGISSVVAS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]