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The map label for this gene is spoT [H]

Identifier: 213158928

GI number: 213158928

Start: 3734126

End: 3736231

Strand: Direct

Name: spoT [H]

Synonym: AB57_3629

Alternate gene names: 213158928

Gene position: 3734126-3736231 (Clockwise)

Preceding gene: 213158927

Following gene: 213158929

Centisome position: 92.19

GC content: 39.36

Gene sequence:

>2106_bases
ATGCCAGGCGAAGAGGTCAGCCAAGCCAAGCAACAGCTCAAATTAATTATCGACCCTTACTTATCGGTAAGTGAAGTCGA
AAAAGTGCTTGCTGCCTGCGATTTTGGAGATCTGGCACACACTGGTATTACCCGTAAAAGTGGAGAACCTTATATTCTGC
ATCCTATCGCGGTTAGCTGTATTTTAGCGAATATGCGTCTGGATCCTGAAACATTAATGGCTGCACTTTTACATGATGTC
ATTGAAGACACTCAATATACAAAAGACGATATTATTGAGCGATTTGGACAAACAGTTGCAGAGCTTGTTGATGGCGTAAC
CAAACTAAGCCAGTCGAGCGATAAAGAATATAATAAAGCTGCTTCTTTTAGAAAAATTCTACAAGCTACTTTGCAGGATC
CACGTGTCATTATTATTAAACTAGCTGATCGCTACCACAATATGACCACATTGGGCGCCTTACGTCCCGATAAACGTGCT
CGTATTGCTCAAGAAACCTTTGATATTTTCGTACCGATGGCAAGATTAGTCGGCATGAATGAAATGGCCGATAACCTTGA
AAATTTATGTTATCAAAATCTCGATTTAGATATGTTTGATAACGTTCAAAATGCGTTGCTTCAAACCAAACCAGAGCGAT
GTAAATACCAAAGTATCTGGGAACAAAATCTTGCGGAGCTTCTCCACAATTATCATATTCAAGGTCGAATTAAAAAGAAA
AATAATAATATTGAGCTCTTACGCCATTTCGTAAAAAATGAAATGGATTTACAAGAACTTACTCATAGCCATGCCTTTGA
AATCGTTTTGCAATCCATTGCAGACTGCGATCGTTTAGTAGCAGCATTAAAAGAAAATTTTCAAGTCATCCAATATCAAG
ATCATATCCGCCGGCCATTACCAGGTGGTAATCAGTCATTAATGATCAAGCTTAAGGGTGAAAAAACCACCTTATCGCTC
ACTATTCAAACTGAGTTGATGCGTAAAGCTGCACGTTTCGGTGTAGTACTCGGCGAAAATGCTCCACAAACTTGCCGCTC
TGCCATTCAAGCTTCCATGCAGAACCTAAATACACTCATTGATGGTGAATGCGCTAAAACGACTTTTAATGACTTACTTG
ATTATTTACATCAAGAAAAAATTTGGGTTTACACACCACATGGCCAGTTACATGAATTACCACAGGGCGCCACAGTAGTT
GATTTTGCCTATTCAGCTAGTCTTTTCTTAGGCAACCATGCGGTAGGTGCAAAAGTTGATGGTGAAATTAAGCCACTTTC
TACACCATTAGTTAGTGGGCAGGTTATTGAGATTATTACTGACGTTTTAGCAACGCCAAATCCAGATTGGCTGAGCTTCA
TTAACACTCAAAAAGCTCGTCGTGCTTTACAGCACGTACTCAAAGACCAAGATATTGAAGAACAACGTTTAGTGGGTGCC
CAAGCACTTTCACGAGCTTTAAAACTCTTTAATCGTTCTATTAACGACCTTTCAGATGCAGATTGGTTAGATCTTTTACA
ATGGCGTCATATTGATAATAAAGATGCATTATTTGAGCAAATTGCTGTTGGAGATTTACTTCCTCAACTTGTAGCTAACC
ATTTGTTTGCAAATGACAAGCATCCTCGAGCAGAAAATTCAGATCGACTAATTCAAGGTACTGAAGGCATTGATGTTAAA
TATGCCCACTGTTGTAATCCTATTTTAGGCGACCCAATTCAAGGCCATTTAACACGACGTGGTTTAATTGTGCACCGTAT
TCGTTGTCATAATTTGCTTCATGAACAACATTTACACCCAGAAAATATTATGCCGCTTCAATGGAAAGCAGATGACGTAG
ATGATGTACGCTTTACTGCTTACCTTGCGATTTACATGGCAATGAATGATGAGCAGGTATCAGACCTCATTTACCAATGT
CGTAAAAATAATGCTGGGGTAGAAATGGTACATTCCAACGAACAGCGTACTTTCGTCAATATTGTAGTAAACAACCGTAA
ACATATTGCAAAAGTAATTCGTGATTTACGAATGCACTATGGCTTCCCACGTATTGAGCGTCTAGACGCCCCTGCTCCAC
AAATGGAAATTTCCAAAGTCAGCTAA

Upstream 100 bases:

>100_bases
ATACACTTTTTCAAACGAAAAAATTGACAAGTTCCGCAATATGCTTTTCATTAAGAAGTAGATAGATTTAGTTTTTTAAG
CAACAGAAAAAGGGTGGTCT

Downstream 100 bases:

>100_bases
AATTTGTTCTGCTTTACTAAAATCTAACTTCTAGAAGTACCTAATCAATTAAATCGATGATACTTTTGTCAAAACGTATC
ATCGATTATGATGAAAAAAG

Product: guanosine-3,5-bis(diphosphate) 3-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MPGEEVSQAKQQLKLIIDPYLSVSEVEKVLAACDFGDLAHTGITRKSGEPYILHPIAVSCILANMRLDPETLMAALLHDV
IEDTQYTKDDIIERFGQTVAELVDGVTKLSQSSDKEYNKAASFRKILQATLQDPRVIIIKLADRYHNMTTLGALRPDKRA
RIAQETFDIFVPMARLVGMNEMADNLENLCYQNLDLDMFDNVQNALLQTKPERCKYQSIWEQNLAELLHNYHIQGRIKKK
NNNIELLRHFVKNEMDLQELTHSHAFEIVLQSIADCDRLVAALKENFQVIQYQDHIRRPLPGGNQSLMIKLKGEKTTLSL
TIQTELMRKAARFGVVLGENAPQTCRSAIQASMQNLNTLIDGECAKTTFNDLLDYLHQEKIWVYTPHGQLHELPQGATVV
DFAYSASLFLGNHAVGAKVDGEIKPLSTPLVSGQVIEIITDVLATPNPDWLSFINTQKARRALQHVLKDQDIEEQRLVGA
QALSRALKLFNRSINDLSDADWLDLLQWRHIDNKDALFEQIAVGDLLPQLVANHLFANDKHPRAENSDRLIQGTEGIDVK
YAHCCNPILGDPIQGHLTRRGLIVHRIRCHNLLHEQHLHPENIMPLQWKADDVDDVRFTAYLAIYMAMNDEQVSDLIYQC
RKNNAGVEMVHSNEQRTFVNIVVNNRKHIAKVIRDLRMHYGFPRIERLDAPAPQMEISKVS

Sequences:

>Translated_701_residues
MPGEEVSQAKQQLKLIIDPYLSVSEVEKVLAACDFGDLAHTGITRKSGEPYILHPIAVSCILANMRLDPETLMAALLHDV
IEDTQYTKDDIIERFGQTVAELVDGVTKLSQSSDKEYNKAASFRKILQATLQDPRVIIIKLADRYHNMTTLGALRPDKRA
RIAQETFDIFVPMARLVGMNEMADNLENLCYQNLDLDMFDNVQNALLQTKPERCKYQSIWEQNLAELLHNYHIQGRIKKK
NNNIELLRHFVKNEMDLQELTHSHAFEIVLQSIADCDRLVAALKENFQVIQYQDHIRRPLPGGNQSLMIKLKGEKTTLSL
TIQTELMRKAARFGVVLGENAPQTCRSAIQASMQNLNTLIDGECAKTTFNDLLDYLHQEKIWVYTPHGQLHELPQGATVV
DFAYSASLFLGNHAVGAKVDGEIKPLSTPLVSGQVIEIITDVLATPNPDWLSFINTQKARRALQHVLKDQDIEEQRLVGA
QALSRALKLFNRSINDLSDADWLDLLQWRHIDNKDALFEQIAVGDLLPQLVANHLFANDKHPRAENSDRLIQGTEGIDVK
YAHCCNPILGDPIQGHLTRRGLIVHRIRCHNLLHEQHLHPENIMPLQWKADDVDDVRFTAYLAIYMAMNDEQVSDLIYQC
RKNNAGVEMVHSNEQRTFVNIVVNNRKHIAKVIRDLRMHYGFPRIERLDAPAPQMEISKVS
>Mature_700_residues
PGEEVSQAKQQLKLIIDPYLSVSEVEKVLAACDFGDLAHTGITRKSGEPYILHPIAVSCILANMRLDPETLMAALLHDVI
EDTQYTKDDIIERFGQTVAELVDGVTKLSQSSDKEYNKAASFRKILQATLQDPRVIIIKLADRYHNMTTLGALRPDKRAR
IAQETFDIFVPMARLVGMNEMADNLENLCYQNLDLDMFDNVQNALLQTKPERCKYQSIWEQNLAELLHNYHIQGRIKKKN
NNIELLRHFVKNEMDLQELTHSHAFEIVLQSIADCDRLVAALKENFQVIQYQDHIRRPLPGGNQSLMIKLKGEKTTLSLT
IQTELMRKAARFGVVLGENAPQTCRSAIQASMQNLNTLIDGECAKTTFNDLLDYLHQEKIWVYTPHGQLHELPQGATVVD
FAYSASLFLGNHAVGAKVDGEIKPLSTPLVSGQVIEIITDVLATPNPDWLSFINTQKARRALQHVLKDQDIEEQRLVGAQ
ALSRALKLFNRSINDLSDADWLDLLQWRHIDNKDALFEQIAVGDLLPQLVANHLFANDKHPRAENSDRLIQGTEGIDVKY
AHCCNPILGDPIQGHLTRRGLIVHRIRCHNLLHEQHLHPENIMPLQWKADDVDDVRFTAYLAIYMAMNDEQVSDLIYQCR
KNNAGVEMVHSNEQRTFVNIVVNNRKHIAKVIRDLRMHYGFPRIERLDAPAPQMEISKVS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=139, Percent_Identity=38.1294964028777, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1790082, Length=693, Percent_Identity=33.1890331890332, Blast_Score=356, Evalue=3e-99,
Organism=Escherichia coli, GI1789147, Length=597, Percent_Identity=24.9581239530988, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI25143118, Length=134, Percent_Identity=38.8059701492537, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79688; Mature: 79557

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGEEVSQAKQQLKLIIDPYLSVSEVEKVLAACDFGDLAHTGITRKSGEPYILHPIAVSC
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEHHHHHHH
ILANMRLDPETLMAALLHDVIEDTQYTKDDIIERFGQTVAELVDGVTKLSQSSDKEYNKA
HHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ASFRKILQATLQDPRVIIIKLADRYHNMTTLGALRPDKRARIAQETFDIFVPMARLVGMN
HHHHHHHHHHHCCCCEEEEEEHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHH
EMADNLENLCYQNLDLDMFDNVQNALLQTKPERCKYQSIWEQNLAELLHNYHIQGRIKKK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
NNNIELLRHFVKNEMDLQELTHSHAFEIVLQSIADCDRLVAALKENFQVIQYQDHIRRPL
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHCCCC
PGGNQSLMIKLKGEKTTLSLTIQTELMRKAARFGVVLGENAPQTCRSAIQASMQNLNTLI
CCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHHHH
DGECAKTTFNDLLDYLHQEKIWVYTPHGQLHELPQGATVVDFAYSASLFLGNHAVGAKVD
CCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCEEEEHHHHHHHEECCCCCCCCCC
GEIKPLSTPLVSGQVIEIITDVLATPNPDWLSFINTQKARRALQHVLKDQDIEEQRLVGA
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QALSRALKLFNRSINDLSDADWLDLLQWRHIDNKDALFEQIAVGDLLPQLVANHLFANDK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
HPRAENSDRLIQGTEGIDVKYAHCCNPILGDPIQGHLTRRGLIVHRIRCHNLLHEQHLHP
CCCCCCCCCEEECCCCCCEEHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCC
ENIMPLQWKADDVDDVRFTAYLAIYMAMNDEQVSDLIYQCRKNNAGVEMVHSNEQRTFVN
CCCCCCEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEE
IVVNNRKHIAKVIRDLRMHYGFPRIERLDAPAPQMEISKVS
EEECCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PGEEVSQAKQQLKLIIDPYLSVSEVEKVLAACDFGDLAHTGITRKSGEPYILHPIAVSC
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEHHHHHHH
ILANMRLDPETLMAALLHDVIEDTQYTKDDIIERFGQTVAELVDGVTKLSQSSDKEYNKA
HHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ASFRKILQATLQDPRVIIIKLADRYHNMTTLGALRPDKRARIAQETFDIFVPMARLVGMN
HHHHHHHHHHHCCCCEEEEEEHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHH
EMADNLENLCYQNLDLDMFDNVQNALLQTKPERCKYQSIWEQNLAELLHNYHIQGRIKKK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
NNNIELLRHFVKNEMDLQELTHSHAFEIVLQSIADCDRLVAALKENFQVIQYQDHIRRPL
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHCCCC
PGGNQSLMIKLKGEKTTLSLTIQTELMRKAARFGVVLGENAPQTCRSAIQASMQNLNTLI
CCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHHHH
DGECAKTTFNDLLDYLHQEKIWVYTPHGQLHELPQGATVVDFAYSASLFLGNHAVGAKVD
CCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCEEEEHHHHHHHEECCCCCCCCCC
GEIKPLSTPLVSGQVIEIITDVLATPNPDWLSFINTQKARRALQHVLKDQDIEEQRLVGA
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QALSRALKLFNRSINDLSDADWLDLLQWRHIDNKDALFEQIAVGDLLPQLVANHLFANDK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
HPRAENSDRLIQGTEGIDVKYAHCCNPILGDPIQGHLTRRGLIVHRIRCHNLLHEQHLHP
CCCCCCCCCEEECCCCCCEEHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCC
ENIMPLQWKADDVDDVRFTAYLAIYMAMNDEQVSDLIYQCRKNNAGVEMVHSNEQRTFVN
CCCCCCEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEE
IVVNNRKHIAKVIRDLRMHYGFPRIERLDAPAPQMEISKVS
EEECCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]