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The map label for this gene is purD [H]

Identifier: 213158572

GI number: 213158572

Start: 2620233

End: 2621519

Strand: Direct

Name: purD [H]

Synonym: AB57_2526

Alternate gene names: 213158572

Gene position: 2620233-2621519 (Clockwise)

Preceding gene: 213158571

Following gene: 213158573

Centisome position: 64.69

GC content: 42.89

Gene sequence:

>1287_bases
ATGAATATTTTAGTTTTAGGTAGTGGTGGTCGTGAGCATGCTTTAGCATGGAAAATCGCGCAAGATGCCAAAGTTACTCA
AGTTTTCGTTGCGCCAGGTAACGCAGGAACTGCAACAGAACCAAAATGCGTTAACGTTCAGCTCGATATTTTAGACAACC
CGGCAATTATTGCTTTTGCTAAAGAAAATAATGTTGAACTGATTGTTGTTGGCCCAGAAGCGCCATTAGTAAATGGTGTT
GTTGATGCTGCGCGTGAAGCAGGCGTAAAAATTTGGGGACCTACTCAATATGCAGCACAGCTTGAAGGTTCAAAAGCATT
TGCTAAGCACTTTTTAAAACGCCACAACATTCCAACTGCTTTTTATGAAGTATTTACGGAAGTTGATGCAGCAAAAGCTT
ATGTTGAAAAAAATGGCGCACCAATTGTAATTAAAGCTGACGGTCTTGCGGCTGGTAAAGGCGTTATCGTTGCAATGACC
AATGAAGAAGCTTTTGCAGCAATTGATGACATGCTTGCAGGCAACAAATTTGGTGATGCAGGCTCACGTGTTGTAATTGA
ACAGTTCCTTGCTGGTGAAGAAGCTTCTTTCATTTGTATGATTGATGGTAAAAACATTTTACCAATGGCAACTTCACAAG
ATCACAAACGTATTTTTGAAGGTGACCAAGGTCCTAACACAGGTGGTATGGGTGCCTACTCTCCTGCTCCAGTTGTGACT
CCAGACGTTTTTGAGCGTGTAATGAATGAAATTATGCGCCCAACTGTTGATGGTATGGCTGCTGATGGACATGTTTATAC
TGGCTTCTTATATGCTGGTTTAATGATTGATGAACAAGGTCAACCACGCGTAATTGAGTTTAACTGCCGTTTCGGTGATC
CGGAAACTCAACCTATCATGATGCGTTTGAAGTCATCTCTTGTTGATTTGGTTGAAGCAGGTATCGCAGGTAACTTACCA
AGCGAAGCAGAATGGGATGAGCGTAAGTCAATCGGTATTGTACTTGCAGCAGAAGGTTACCCAGACAGCGTGCGTAAAGG
TGACGTTATCTCAGGTATCGGTCAATCACCAGAAGATACTAAAATCTTCCATGCAGGTACTGCTACTCGTGAAGACGGCC
ATATTGTTACTTCTGGCGGACGTGTGCTTTGCGTGACTGCTCTAGGTGACAGCGTTCTTGAAGCACAAATCAATGCTTTA
GAAGTATGTGGGCAAGTAACCTTCACAGGTATGCAATACCGCAGTGACATCGGTTACCGTGCTATTGCTCGTGAAAAAGC
TGAATAA

Upstream 100 bases:

>100_bases
CCTTTTATTCAGGAGGGTTTTCCACAATAATGGAACTATCTCTCCACCTTGAGAAATAGAGGGGGATGAAAAAGGATTAA
AGTTAAGGAACAAATTAAAA

Downstream 100 bases:

>100_bases
AAATTTAAATAGTAAAAAAGCTCTCTTATGAGGGCTTTTTTATTTTGCTTATTCACTTATCACAAATATTCATATTAATC
TTAATTTTTATTCAATTCTG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNILVLGSGGREHALAWKIAQDAKVTQVFVAPGNAGTATEPKCVNVQLDILDNPAIIAFAKENNVELIVVGPEAPLVNGV
VDAAREAGVKIWGPTQYAAQLEGSKAFAKHFLKRHNIPTAFYEVFTEVDAAKAYVEKNGAPIVIKADGLAAGKGVIVAMT
NEEAFAAIDDMLAGNKFGDAGSRVVIEQFLAGEEASFICMIDGKNILPMATSQDHKRIFEGDQGPNTGGMGAYSPAPVVT
PDVFERVMNEIMRPTVDGMAADGHVYTGFLYAGLMIDEQGQPRVIEFNCRFGDPETQPIMMRLKSSLVDLVEAGIAGNLP
SEAEWDERKSIGIVLAAEGYPDSVRKGDVISGIGQSPEDTKIFHAGTATREDGHIVTSGGRVLCVTALGDSVLEAQINAL
EVCGQVTFTGMQYRSDIGYRAIAREKAE

Sequences:

>Translated_428_residues
MNILVLGSGGREHALAWKIAQDAKVTQVFVAPGNAGTATEPKCVNVQLDILDNPAIIAFAKENNVELIVVGPEAPLVNGV
VDAAREAGVKIWGPTQYAAQLEGSKAFAKHFLKRHNIPTAFYEVFTEVDAAKAYVEKNGAPIVIKADGLAAGKGVIVAMT
NEEAFAAIDDMLAGNKFGDAGSRVVIEQFLAGEEASFICMIDGKNILPMATSQDHKRIFEGDQGPNTGGMGAYSPAPVVT
PDVFERVMNEIMRPTVDGMAADGHVYTGFLYAGLMIDEQGQPRVIEFNCRFGDPETQPIMMRLKSSLVDLVEAGIAGNLP
SEAEWDERKSIGIVLAAEGYPDSVRKGDVISGIGQSPEDTKIFHAGTATREDGHIVTSGGRVLCVTALGDSVLEAQINAL
EVCGQVTFTGMQYRSDIGYRAIAREKAE
>Mature_428_residues
MNILVLGSGGREHALAWKIAQDAKVTQVFVAPGNAGTATEPKCVNVQLDILDNPAIIAFAKENNVELIVVGPEAPLVNGV
VDAAREAGVKIWGPTQYAAQLEGSKAFAKHFLKRHNIPTAFYEVFTEVDAAKAYVEKNGAPIVIKADGLAAGKGVIVAMT
NEEAFAAIDDMLAGNKFGDAGSRVVIEQFLAGEEASFICMIDGKNILPMATSQDHKRIFEGDQGPNTGGMGAYSPAPVVT
PDVFERVMNEIMRPTVDGMAADGHVYTGFLYAGLMIDEQGQPRVIEFNCRFGDPETQPIMMRLKSSLVDLVEAGIAGNLP
SEAEWDERKSIGIVLAAEGYPDSVRKGDVISGIGQSPEDTKIFHAGTATREDGHIVTSGGRVLCVTALGDSVLEAQINAL
EVCGQVTFTGMQYRSDIGYRAIAREKAE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=48.5915492957746, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=48.5915492957746, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=48.5915492957746, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=48.5915492957746, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=64.018691588785, Blast_Score=543, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17567511, Length=428, Percent_Identity=43.4579439252336, Blast_Score=313, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=42.5055928411633, Blast_Score=339, Evalue=4e-94,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=44.8837209302326, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=44.8837209302326, Blast_Score=346, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45600; Mature: 45600

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50975 ATP_GRASP ; PS00178 AA_TRNA_LIGASE_I ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVLGSGGREHALAWKIAQDAKVTQVFVAPGNAGTATEPKCVNVQLDILDNPAIIAFA
CEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCEEEEEEEEEECCCEEEEEE
KENNVELIVVGPEAPLVNGVVDAAREAGVKIWGPTQYAAQLEGSKAFAKHFLKRHNIPTA
ECCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCHH
FYEVFTEVDAAKAYVEKNGAPIVIKADGLAAGKGVIVAMTNEEAFAAIDDMLAGNKFGDA
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCC
GSRVVIEQFLAGEEASFICMIDGKNILPMATSQDHKRIFEGDQGPNTGGMGAYSPAPVVT
CCHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCC
PDVFERVMNEIMRPTVDGMAADGHVYTGFLYAGLMIDEQGQPRVIEFNCRFGDPETQPIM
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEEECCCCCCCHHH
MRLKSSLVDLVEAGIAGNLPSEAEWDERKSIGIVLAAEGYPDSVRKGDVISGIGQSPEDT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
KIFHAGTATREDGHIVTSGGRVLCVTALGDSVLEAQINALEVCGQVTFTGMQYRSDIGYR
EEEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHCCEEEECEEHHCCCCHH
AIAREKAE
HHHHCCCC
>Mature Secondary Structure
MNILVLGSGGREHALAWKIAQDAKVTQVFVAPGNAGTATEPKCVNVQLDILDNPAIIAFA
CEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCEEEEEEEEEECCCEEEEEE
KENNVELIVVGPEAPLVNGVVDAAREAGVKIWGPTQYAAQLEGSKAFAKHFLKRHNIPTA
ECCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCHH
FYEVFTEVDAAKAYVEKNGAPIVIKADGLAAGKGVIVAMTNEEAFAAIDDMLAGNKFGDA
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCC
GSRVVIEQFLAGEEASFICMIDGKNILPMATSQDHKRIFEGDQGPNTGGMGAYSPAPVVT
CCHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCC
PDVFERVMNEIMRPTVDGMAADGHVYTGFLYAGLMIDEQGQPRVIEFNCRFGDPETQPIM
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEEECCCCCCCHHH
MRLKSSLVDLVEAGIAGNLPSEAEWDERKSIGIVLAAEGYPDSVRKGDVISGIGQSPEDT
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
KIFHAGTATREDGHIVTSGGRVLCVTALGDSVLEAQINALEVCGQVTFTGMQYRSDIGYR
EEEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHCCEEEECEEHHCCCCHH
AIAREKAE
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]