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The map label for this gene is purH [H]

Identifier: 213158571

GI number: 213158571

Start: 2618533

End: 2620107

Strand: Direct

Name: purH [H]

Synonym: AB57_2525

Alternate gene names: 213158571

Gene position: 2618533-2620107 (Clockwise)

Preceding gene: 213158570

Following gene: 213158572

Centisome position: 64.65

GC content: 41.59

Gene sequence:

>1575_bases
ATGACTATTAAACGTGCTTTGATTTCTGTATCAGACAAAACAGGAATTGTCGAATTTGCTCAAAACCTTGCAGCTTTAGG
GGTAGAATTATTATCTACAGGCGGCACATACAAGTTGCTTAAAGATAATAATATTGCTGTTGTTGAAGTATCTGAACATA
CCGGTTTCCCGGAAATGATGGATGGCCGTGTTAAAACACTACATCCAAAAATTCATGGCGGTATTTTGGCTCGTCGTGGT
CTAGACGAAGCTGTTATGCAAGAACACAACATCGATCCAATCGATTTAGTTGTAGTTAACCTTTACCCATTTGCGGCTAC
AGTAGCTAAACCTAACTGTTCTCTTGCTGATGCTATCGAAAATATCGACATCGGTGGTCCAACAATGGTACGTGCTGCTG
CTAAGAACCATGCATCTGTCGGTATTGTGGTAAATGCTTCAGATTACGATACAGTTATTAGCGAATTAAAAGCTTCTGGT
TCACTTTCTTACGAAACACGCTTCGATTTAGCGGTTAAAGCATTTGAACATACAGCTCAATATGACGGCATGATTGCTTC
TTACTTAGGCGCTCGCGTTGGTAAGGCCGAAGGTGAAGCAGATTTATTCCCTCGTACGTTCAATACCCAATTAAACAAAG
CACAAGATTTACGTTACGGCGAAAATCCACACCAATCTGCTGCTTTCTATGTTGAAGCAAATGCAAAAGAAGCTTCTGTT
TCTACTGCAAAACAATTACAAGGCAAAGAATTGTCTTATAACAACATTGCTGATACTGATGCTGCCCTTGAGTGCGTGAA
ATCATTTGCAAAACCAGCTTGTGTCATCGTAAAACATGCTAACCCTTGTGGTGTTGCCGTTTCTTTAGACGGTATTAAAG
CTGCTTATGATTTAGCTTACGCAACAGATCCAGAATCTGCTTTCGGCGGTATTATTGCATTTAACCGTGAGTTAGATGTT
GCTACTGCCCAAGCAATTGTAGACCGCCAATTCGTTGAAGTAATTATTGCTCCAAGCATTGCTGATGGTGTACTTGAAGT
TACTGGCGCGAAGAAAAATGTTCGTGTTTTAGTATGTGGTGAATTACCTGCAATTGATGCACGTGCTCCACAACTTGACT
ATAAACGCGTTAACGGCGGCTTACTTGTTCAAGACCAAGACTTGGGCATGATTACTAAAGATGATCTTAAAGTTGTGACT
AAACGTGCACCAACCGAACAAGAAATTGATGACCTTATTTTCGCATGGAAAGTTGCGAAATACGTAAAATCAAACGCTAT
TGTTTATGCCAAAAACCGTCAAACTATTGGTGTAGGTGCAGGCCAAATGAGCCGTGTTAACTCAGCACGTATCGCAGCAA
TTAAAGCTGAGCATGCTGGTCTGGTTGTTGAAGGCGCAGTTATGGCATCTGATGCATTCTTCCCGTTCCGTGACGGTATT
GATAATGCAGCAAAAGCTGGCATTAAATGCATTATTCAACCGGGTGGTTCTATGCGTGATGAAGAAGTTATTGCAGCTGC
TGATGAAGCTGGCATTGCAATGGTCTTCACTGGTATGCGCCATTTCCGCCACTAA

Upstream 100 bases:

>100_bases
TGAGTGAATAAATGATAAAATGCTCACGGCTTCGTGGGCATTTTTTTTGTGCTTCTAATTGGTTTTAATCCGTAGACTTG
AAATTGTTGACTTGAACATC

Downstream 100 bases:

>100_bases
TTTAAATTTAGTCCTCCCTAACCCTCCTTTTATTCAGGAGGGTTTTCCACAATAATGGAACTATCTCTCCACCTTGAGAA
ATAGAGGGGGATGAAAAAGG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTIKRALISVSDKTGIVEFAQNLAALGVELLSTGGTYKLLKDNNIAVVEVSEHTGFPEMMDGRVKTLHPKIHGGILARRG
LDEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIENIDIGGPTMVRAAAKNHASVGIVVNASDYDTVISELKASG
SLSYETRFDLAVKAFEHTAQYDGMIASYLGARVGKAEGEADLFPRTFNTQLNKAQDLRYGENPHQSAAFYVEANAKEASV
STAKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAYATDPESAFGGIIAFNRELDV
ATAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCGELPAIDARAPQLDYKRVNGGLLVQDQDLGMITKDDLKVVT
KRAPTEQEIDDLIFAWKVAKYVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGI
DNAAKAGIKCIIQPGGSMRDEEVIAAADEAGIAMVFTGMRHFRH

Sequences:

>Translated_524_residues
MTIKRALISVSDKTGIVEFAQNLAALGVELLSTGGTYKLLKDNNIAVVEVSEHTGFPEMMDGRVKTLHPKIHGGILARRG
LDEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIENIDIGGPTMVRAAAKNHASVGIVVNASDYDTVISELKASG
SLSYETRFDLAVKAFEHTAQYDGMIASYLGARVGKAEGEADLFPRTFNTQLNKAQDLRYGENPHQSAAFYVEANAKEASV
STAKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAYATDPESAFGGIIAFNRELDV
ATAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCGELPAIDARAPQLDYKRVNGGLLVQDQDLGMITKDDLKVVT
KRAPTEQEIDDLIFAWKVAKYVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGI
DNAAKAGIKCIIQPGGSMRDEEVIAAADEAGIAMVFTGMRHFRH
>Mature_523_residues
TIKRALISVSDKTGIVEFAQNLAALGVELLSTGGTYKLLKDNNIAVVEVSEHTGFPEMMDGRVKTLHPKIHGGILARRGL
DEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIENIDIGGPTMVRAAAKNHASVGIVVNASDYDTVISELKASGS
LSYETRFDLAVKAFEHTAQYDGMIASYLGARVGKAEGEADLFPRTFNTQLNKAQDLRYGENPHQSAAFYVEANAKEASVS
TAKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAYATDPESAFGGIIAFNRELDVA
TAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCGELPAIDARAPQLDYKRVNGGLLVQDQDLGMITKDDLKVVTK
RAPTEQEIDDLIFAWKVAKYVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGID
NAAKAGIKCIIQPGGSMRDEEVIAAADEAGIAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=507, Percent_Identity=39.4477317554241, Blast_Score=287, Evalue=2e-77,
Organism=Escherichia coli, GI1790439, Length=527, Percent_Identity=70.0189753320683, Blast_Score=750, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=511, Percent_Identity=36.5949119373777, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI71985574, Length=343, Percent_Identity=29.4460641399417, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=50, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323768, Length=484, Percent_Identity=37.1900826446281, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6323056, Length=487, Percent_Identity=36.7556468172485, Blast_Score=266, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24649832, Length=507, Percent_Identity=39.0532544378698, Blast_Score=291, Evalue=1e-78,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56038; Mature: 55906

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKRALISVSDKTGIVEFAQNLAALGVELLSTGGTYKLLKDNNIAVVEVSEHTGFPEMM
CCCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCHHHH
DGRVKTLHPKIHGGILARRGLDEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIE
CCCEEEECCHHHCCHHHHCCCCHHHHHHCCCCCEEEEEEEEECCEEEECCCCCCHHHHHH
NIDIGGPTMVRAAAKNHASVGIVVNASDYDTVISELKASGSLSYETRFDLAVKAFEHTAQ
HCCCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
YDGMIASYLGARVGKAEGEADLFPRTFNTQLNKAQDLRYGENPHQSAAFYVEANAKEASV
HCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHH
STAKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAY
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCHHHHEEE
ATDPESAFGGIIAFNRELDVATAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCG
ECCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCCEEEEEEC
ELPAIDARAPQLDYKRVNGGLLVQDQDLGMITKDDLKVVTKRAPTEQEIDDLIFAWKVAK
CCCCCCCCCCCCCCEECCCCEEEEECCCCCEECCHHHHHHCCCCCHHHHHHHHHHHHHHH
YVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGI
HHHCCEEEEEECCEEEEECCCCHHHCCCCEEEEEEECCCCEEEECEEEECCCCCCHHCCC
DNAAKAGIKCIIQPGGSMRDEEVIAAADEAGIAMVFTGMRHFRH
CHHHCCCEEEEECCCCCCCCCCEEEECCCCCEEHEEHHHHHHCC
>Mature Secondary Structure 
TIKRALISVSDKTGIVEFAQNLAALGVELLSTGGTYKLLKDNNIAVVEVSEHTGFPEMM
CCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCHHHH
DGRVKTLHPKIHGGILARRGLDEAVMQEHNIDPIDLVVVNLYPFAATVAKPNCSLADAIE
CCCEEEECCHHHCCHHHHCCCCHHHHHHCCCCCEEEEEEEEECCEEEECCCCCCHHHHHH
NIDIGGPTMVRAAAKNHASVGIVVNASDYDTVISELKASGSLSYETRFDLAVKAFEHTAQ
HCCCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
YDGMIASYLGARVGKAEGEADLFPRTFNTQLNKAQDLRYGENPHQSAAFYVEANAKEASV
HCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHH
STAKQLQGKELSYNNIADTDAALECVKSFAKPACVIVKHANPCGVAVSLDGIKAAYDLAY
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCHHHHEEE
ATDPESAFGGIIAFNRELDVATAQAIVDRQFVEVIIAPSIADGVLEVTGAKKNVRVLVCG
ECCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCCEEEEEEC
ELPAIDARAPQLDYKRVNGGLLVQDQDLGMITKDDLKVVTKRAPTEQEIDDLIFAWKVAK
CCCCCCCCCCCCCCEECCCCEEEEECCCCCEECCHHHHHHCCCCCHHHHHHHHHHHHHHH
YVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLVVEGAVMASDAFFPFRDGI
HHHCCEEEEEECCEEEEECCCCHHHCCCCEEEEEEECCCCEEEECEEEECCCCCCHHCCC
DNAAKAGIKCIIQPGGSMRDEEVIAAADEAGIAMVFTGMRHFRH
CHHHCCCEEEEECCCCCCCCCCEEEECCCCCEEHEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA