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The map label for this gene is pgaB [H]

Identifier: 213158545

GI number: 213158545

Start: 2590096

End: 2591925

Strand: Reverse

Name: pgaB [H]

Synonym: AB57_2497

Alternate gene names: 213158545

Gene position: 2591925-2590096 (Counterclockwise)

Preceding gene: 213158546

Following gene: 213158544

Centisome position: 63.99

GC content: 37.32

Gene sequence:

>1830_bases
ATGAATAATTTATTGTCGAAAATGTTGGGCTGTGCCTTGGTCTCAACATTGTTGCCTCTGCAATTTGCATATGCCCAGAT
AGAAAAAGATTTACCAAAAAATCATACGGTTTCTTTAACCTTTCATGATGTCAGGGATGATGTCTTAAAAGAAGGGGATC
GGGATATTTATGCAATCCAAACGAAAAATTTAGCACAGTTCTTTGACTGGTTAAGTCAATCTGATTGGAAGCCCATTCGC
TTAAAAGATATTGAAGAAGCGCGTAAACAAGGTAAGGAATTACCTCATAATTCTATTTTGTTAACTTTCGATGATGGTGC
TTTAAGTAGTTATAGCCGTGTATTTCCATTACTTAAGCAATATCAGATTCCGGCAGTATTTGCACTTCCGACCAGCTGGC
TAAATGGGAATACACAAGCTGGCTATGAAGCTTATGGGCAAGGAAATCTAGTCAATTGGAAGCAAGTGCGAGAAATGCAA
GCTTCGGGCTTAGCCGAGTTTGCAAGTCATAGTGATGATTTGCATCATGGAGTATTGGCTAATCCCCAAGGGAATGAACA
GCCTGCTGCGACTTCTTACGCGTATTTAAAACCCCAAAAGCGTTATGAAACGGATGTGGAATATCAACAACGTATTTTGC
AGGACTTAAAAAAATCATACGCCGTTTTAAAGAAAGAAGTGGGTGTTGAACCTAAGGCAATTATTTGGCCTTACGGTGCT
GTGAATGAGCAATTAGAAAAATTGTCTCAAGAAGCCGGCTTTATTTTTTCTTTCAGTTTAGGGCGTGATGGAATGAATAG
AGTGGGTGACTCGACTTTTAAACGTAGTTTGGTCACTAATAATCCTACAGCTGAGCAATTAACAGAGGGTATGATCAATA
TTCTCAACTTTGAAGAATTAGATTTATTTAAGCAACCTCGCCACTTTGTGAGTATGGACTTAAAACAATTAGCTGCTTCA
ACTAATACTCAGTCAGATGAGAAACTAGGATTATTGTTATCCAAGCTTTATAGCTTAAAAAATAACACATTGATATTGAA
AACTTTAGATGATCAAGATGGCGATGGGCAGTATGACATTGCTTATTTCCCAACAACTCAACTAAGCGTCCAGCAAGATA
TTTTGAACCGAACTTTATGGCAGGCACAAACTCGTGCCGGACAGTCGGTTATTTTAGAGTTGCCTGTATATCCTCAAAAA
AATAAACCGTTTTTGGTGGCAGACTTAGCGAAGGATATCGCACGCTTCAATAGTAATTTAAGTGGTATTCAACTTAATGC
TGGAACCACTTTAAACTGTGCTATGCAAAGCAAAACAATAAAAGAAAATGCCTGTGCCGACCAATTAAAACAGCTCACAT
ATGTGAGCCAGCTTACTCAAAAGGCTGTGAAGCCTTATTTAAACATGAGTAATCAGGCTCAGTTTAGTTTATTGCTTACA
CCTGATTTTGAACATATCGAAAATTTACCAACGCTTTTAAAAACGCTGTTATCCCAACATGATTTAGTAAATCTTAAGTT
CAATATAGTGGGTAAGCAAAAACAGTTTAATCATGTATTAGCAATTTTAAATACGCTTGATTCGAAATATAAACAGCGCA
TTATGCTGACTTTATCATTACCCGAAAATAGTCAGCAAAATGTCTGGCAAGAAGTGAAACAAGGTCTTTTCAATATTCAG
CGCATAGGTATTCAGAAATTTGGTGTTGATGGCTATACCAATGAAAACTCAAAAAATGTTCATGAATATTTATATAACCC
GATTAGCTTGAATTCGAGTTCGATCATGTATCAGCCCTTTGCAGGTCTCGCGACTGAGGGGAAAAAGTAA

Upstream 100 bases:

>100_bases
CATGGCAGCTCAATTATGGAATTGGTTGGCAATACCATCCTTACGATGGACATGATGAACAACATACCTACGGTATTTTT
GGATTTGAAGGGCGTTTTTA

Downstream 100 bases:

>100_bases
TGAAAGTATTACTCGATATTTTATTTGCTTTTGCCTTTTTATATCCGTTGTTGATGGCTTGGACATGGATGGTAGGAGGC
CTCTGGTTTTTCTTTAAAAG

Product: polysaccharide deacetylase

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MNNLLSKMLGCALVSTLLPLQFAYAQIEKDLPKNHTVSLTFHDVRDDVLKEGDRDIYAIQTKNLAQFFDWLSQSDWKPIR
LKDIEEARKQGKELPHNSILLTFDDGALSSYSRVFPLLKQYQIPAVFALPTSWLNGNTQAGYEAYGQGNLVNWKQVREMQ
ASGLAEFASHSDDLHHGVLANPQGNEQPAATSYAYLKPQKRYETDVEYQQRILQDLKKSYAVLKKEVGVEPKAIIWPYGA
VNEQLEKLSQEAGFIFSFSLGRDGMNRVGDSTFKRSLVTNNPTAEQLTEGMINILNFEELDLFKQPRHFVSMDLKQLAAS
TNTQSDEKLGLLLSKLYSLKNNTLILKTLDDQDGDGQYDIAYFPTTQLSVQQDILNRTLWQAQTRAGQSVILELPVYPQK
NKPFLVADLAKDIARFNSNLSGIQLNAGTTLNCAMQSKTIKENACADQLKQLTYVSQLTQKAVKPYLNMSNQAQFSLLLT
PDFEHIENLPTLLKTLLSQHDLVNLKFNIVGKQKQFNHVLAILNTLDSKYKQRIMLTLSLPENSQQNVWQEVKQGLFNIQ
RIGIQKFGVDGYTNENSKNVHEYLYNPISLNSSSIMYQPFAGLATEGKK

Sequences:

>Translated_609_residues
MNNLLSKMLGCALVSTLLPLQFAYAQIEKDLPKNHTVSLTFHDVRDDVLKEGDRDIYAIQTKNLAQFFDWLSQSDWKPIR
LKDIEEARKQGKELPHNSILLTFDDGALSSYSRVFPLLKQYQIPAVFALPTSWLNGNTQAGYEAYGQGNLVNWKQVREMQ
ASGLAEFASHSDDLHHGVLANPQGNEQPAATSYAYLKPQKRYETDVEYQQRILQDLKKSYAVLKKEVGVEPKAIIWPYGA
VNEQLEKLSQEAGFIFSFSLGRDGMNRVGDSTFKRSLVTNNPTAEQLTEGMINILNFEELDLFKQPRHFVSMDLKQLAAS
TNTQSDEKLGLLLSKLYSLKNNTLILKTLDDQDGDGQYDIAYFPTTQLSVQQDILNRTLWQAQTRAGQSVILELPVYPQK
NKPFLVADLAKDIARFNSNLSGIQLNAGTTLNCAMQSKTIKENACADQLKQLTYVSQLTQKAVKPYLNMSNQAQFSLLLT
PDFEHIENLPTLLKTLLSQHDLVNLKFNIVGKQKQFNHVLAILNTLDSKYKQRIMLTLSLPENSQQNVWQEVKQGLFNIQ
RIGIQKFGVDGYTNENSKNVHEYLYNPISLNSSSIMYQPFAGLATEGKK
>Mature_609_residues
MNNLLSKMLGCALVSTLLPLQFAYAQIEKDLPKNHTVSLTFHDVRDDVLKEGDRDIYAIQTKNLAQFFDWLSQSDWKPIR
LKDIEEARKQGKELPHNSILLTFDDGALSSYSRVFPLLKQYQIPAVFALPTSWLNGNTQAGYEAYGQGNLVNWKQVREMQ
ASGLAEFASHSDDLHHGVLANPQGNEQPAATSYAYLKPQKRYETDVEYQQRILQDLKKSYAVLKKEVGVEPKAIIWPYGA
VNEQLEKLSQEAGFIFSFSLGRDGMNRVGDSTFKRSLVTNNPTAEQLTEGMINILNFEELDLFKQPRHFVSMDLKQLAAS
TNTQSDEKLGLLLSKLYSLKNNTLILKTLDDQDGDGQYDIAYFPTTQLSVQQDILNRTLWQAQTRAGQSVILELPVYPQK
NKPFLVADLAKDIARFNSNLSGIQLNAGTTLNCAMQSKTIKENACADQLKQLTYVSQLTQKAVKPYLNMSNQAQFSLLLT
PDFEHIENLPTLLKTLLSQHDLVNLKFNIVGKQKQFNHVLAILNTLDSKYKQRIMLTLSLPENSQQNVWQEVKQGLFNIQ
RIGIQKFGVDGYTNENSKNVHEYLYNPISLNSSSIMYQPFAGLATEGKK

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=377, Percent_Identity=32.3607427055703, Blast_Score=218, Evalue=9e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 68905; Mature: 68905

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLLSKMLGCALVSTLLPLQFAYAQIEKDLPKNHTVSLTFHDVRDDVLKEGDRDIYAIQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCCEEEEE
TKNLAQFFDWLSQSDWKPIRLKDIEEARKQGKELPHNSILLTFDDGALSSYSRVFPLLKQ
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
YQIPAVFALPTSWLNGNTQAGYEAYGQGNLVNWKQVREMQASGLAEFASHSDDLHHGVLA
CCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEC
NPQGNEQPAATSYAYLKPQKRYETDVEYQQRILQDLKKSYAVLKKEVGVEPKAIIWPYGA
CCCCCCCCCCCCCCEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCH
VNEQLEKLSQEAGFIFSFSLGRDGMNRVGDSTFKRSLVTNNPTAEQLTEGMINILNFEEL
HHHHHHHHHHHCCEEEEEECCCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
DLFKQPRHFVSMDLKQLAASTNTQSDEKLGLLLSKLYSLKNNTLILKTLDDQDGDGQYDI
HHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEE
AYFPTTQLSVQQDILNRTLWQAQTRAGQSVILELPVYPQKNKPFLVADLAKDIARFNSNL
EECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHCCCC
SGIQLNAGTTLNCAMQSKTIKENACADQLKQLTYVSQLTQKAVKPYLNMSNQAQFSLLLT
CCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PDFEHIENLPTLLKTLLSQHDLVNLKFNIVGKQKQFNHVLAILNTLDSKYKQRIMLTLSL
CCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCEEEEEEEC
PENSQQNVWQEVKQGLFNIQRIGIQKFGVDGYTNENSKNVHEYLYNPISLNSSSIMYQPF
CCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCC
AGLATEGKK
CCCCCCCCC
>Mature Secondary Structure
MNNLLSKMLGCALVSTLLPLQFAYAQIEKDLPKNHTVSLTFHDVRDDVLKEGDRDIYAIQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCCEEEEE
TKNLAQFFDWLSQSDWKPIRLKDIEEARKQGKELPHNSILLTFDDGALSSYSRVFPLLKQ
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
YQIPAVFALPTSWLNGNTQAGYEAYGQGNLVNWKQVREMQASGLAEFASHSDDLHHGVLA
CCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEC
NPQGNEQPAATSYAYLKPQKRYETDVEYQQRILQDLKKSYAVLKKEVGVEPKAIIWPYGA
CCCCCCCCCCCCCCEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCH
VNEQLEKLSQEAGFIFSFSLGRDGMNRVGDSTFKRSLVTNNPTAEQLTEGMINILNFEEL
HHHHHHHHHHHCCEEEEEECCCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
DLFKQPRHFVSMDLKQLAASTNTQSDEKLGLLLSKLYSLKNNTLILKTLDDQDGDGQYDI
HHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEE
AYFPTTQLSVQQDILNRTLWQAQTRAGQSVILELPVYPQKNKPFLVADLAKDIARFNSNL
EECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHCCCC
SGIQLNAGTTLNCAMQSKTIKENACADQLKQLTYVSQLTQKAVKPYLNMSNQAQFSLLLT
CCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
PDFEHIENLPTLLKTLLSQHDLVNLKFNIVGKQKQFNHVLAILNTLDSKYKQRIMLTLSL
CCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCEEEEEEEC
PENSQQNVWQEVKQGLFNIQRIGIQKFGVDGYTNENSKNVHEYLYNPISLNSSSIMYQPF
CCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCC
AGLATEGKK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]