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The map label for this gene is pgaC [H]

Identifier: 213158544

GI number: 213158544

Start: 2588849

End: 2590096

Strand: Reverse

Name: pgaC [H]

Synonym: AB57_2496

Alternate gene names: 213158544

Gene position: 2590096-2588849 (Counterclockwise)

Preceding gene: 213158545

Following gene: 213158543

Centisome position: 63.94

GC content: 38.94

Gene sequence:

>1248_bases
ATGAAAGTATTACTCGATATTTTATTTGCTTTTGCCTTTTTATATCCGTTGTTGATGGCTTGGACATGGATGGTAGGAGG
CCTCTGGTTTTTCTTTAAAAGGGAATATCATGAGCAGCAACTGCCTGAGCCATCAAGTGAAGGGTGCAGTATTATTATTC
CTTGCTTTAATGAAGTAGCTCAGGTCCGACAAACGATTCGTTATGCCTTACAGACAAAATATCCGAATTTTGAAGTCATT
GCTGTAAATGATGGAAGTAGTGACAGCACTGCAGAAATTCTTGATGAACTGGCAGCTCAAGATGCCCGATTAAGAGTTGT
GCACCTGGCAGAAAACCAAGGTAAGGCCGTAGCTTTAAGATCGGGAGTGTTAGTAAGTAAATACGAATATCTAGTTTGTA
TTGACGGAGATGCTTTATTACATCCTCATGCAGTACTTTGGCTTATGCAGCCTTTTTTAAATTTTCCAAGAATTGGTGCT
GTAACTGGTAATCCTAGAATTTTAAATCGCTCAAGTATTTTAGGTAAATTACAAGTTGGCGAGTTCTCTTCAATAATTGG
CTTGATTAAGCGCGCACAAAGAACATATGGCCGTATTTTTACAGTATCAGGTGTAATTGCAGCATTTAGAAAGACCGCTT
TGGTTCGCGTGGGCTTTTGGTCTGATGACAAAATTACGGAAGATATTGATATCTCTTGGAAGCTCCAAATGGACCATTGG
GATATTCAATATATTCCTCAAGCACTTTGTTATATCTATATGCCGGAAACCTTTAAAGGCCTTTGGAAACAGCGTTTACG
CTGGGCACAAGGTGGGGTAGAGGTATTATTAGAATATATTCCGAAAATGTTCAAACTCCGCTTGCGCCGTATGTGGCCGG
TAATGCTCGAAGCTTTAGTCAGCATTATTTGGTCATATGTGATGATTATGATTTTTATTCTATTTTTTGTGGGATTGTTT
GTGGATTTACCGCAACAATTTCAAATTAATTCGCTCATGCCGCAGTGGTATGGCGTGATATTAGGTGGAACATGTCTTGT
TCAATTTCTAGTAAGTTTATGGATAGATCATCGTTATGATCGTGGTCGGCTTTTTAGAAATTATTTTTGGGTAATTTGGT
ATCCATTATTTTTCTGGTTACTGACGTTATTTACCAGTGTTGTTGCAGTACCCAAAACTATATTTAACACCAAAAAACGT
GCTCGTTGGGTAAGCCCTGATCGAGGCTTCCGTGGAGATCACTCATGA

Upstream 100 bases:

>100_bases
CAAAAAATGTTCATGAATATTTATATAACCCGATTAGCTTGAATTCGAGTTCGATCATGTATCAGCCCTTTGCAGGTCTC
GCGACTGAGGGGAAAAAGTA

Downstream 100 bases:

>100_bases
AAAATGATACAAATATCGACGTAGAAGTTTTAGATATACCTGAATATATTGATCAGCCTGAATATGTGAAAAATAAAACT
GCAAACTATACGTTACAAAC

Product: glycosyl transferase, family 2 protein

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKVLLDILFAFAFLYPLLMAWTWMVGGLWFFFKREYHEQQLPEPSSEGCSIIIPCFNEVAQVRQTIRYALQTKYPNFEVI
AVNDGSSDSTAEILDELAAQDARLRVVHLAENQGKAVALRSGVLVSKYEYLVCIDGDALLHPHAVLWLMQPFLNFPRIGA
VTGNPRILNRSSILGKLQVGEFSSIIGLIKRAQRTYGRIFTVSGVIAAFRKTALVRVGFWSDDKITEDIDISWKLQMDHW
DIQYIPQALCYIYMPETFKGLWKQRLRWAQGGVEVLLEYIPKMFKLRLRRMWPVMLEALVSIIWSYVMIMIFILFFVGLF
VDLPQQFQINSLMPQWYGVILGGTCLVQFLVSLWIDHRYDRGRLFRNYFWVIWYPLFFWLLTLFTSVVAVPKTIFNTKKR
ARWVSPDRGFRGDHS

Sequences:

>Translated_415_residues
MKVLLDILFAFAFLYPLLMAWTWMVGGLWFFFKREYHEQQLPEPSSEGCSIIIPCFNEVAQVRQTIRYALQTKYPNFEVI
AVNDGSSDSTAEILDELAAQDARLRVVHLAENQGKAVALRSGVLVSKYEYLVCIDGDALLHPHAVLWLMQPFLNFPRIGA
VTGNPRILNRSSILGKLQVGEFSSIIGLIKRAQRTYGRIFTVSGVIAAFRKTALVRVGFWSDDKITEDIDISWKLQMDHW
DIQYIPQALCYIYMPETFKGLWKQRLRWAQGGVEVLLEYIPKMFKLRLRRMWPVMLEALVSIIWSYVMIMIFILFFVGLF
VDLPQQFQINSLMPQWYGVILGGTCLVQFLVSLWIDHRYDRGRLFRNYFWVIWYPLFFWLLTLFTSVVAVPKTIFNTKKR
ARWVSPDRGFRGDHS
>Mature_415_residues
MKVLLDILFAFAFLYPLLMAWTWMVGGLWFFFKREYHEQQLPEPSSEGCSIIIPCFNEVAQVRQTIRYALQTKYPNFEVI
AVNDGSSDSTAEILDELAAQDARLRVVHLAENQGKAVALRSGVLVSKYEYLVCIDGDALLHPHAVLWLMQPFLNFPRIGA
VTGNPRILNRSSILGKLQVGEFSSIIGLIKRAQRTYGRIFTVSGVIAAFRKTALVRVGFWSDDKITEDIDISWKLQMDHW
DIQYIPQALCYIYMPETFKGLWKQRLRWAQGGVEVLLEYIPKMFKLRLRRMWPVMLEALVSIIWSYVMIMIFILFFVGLF
VDLPQQFQINSLMPQWYGVILGGTCLVQFLVSLWIDHRYDRGRLFRNYFWVIWYPLFFWLLTLFTSVVAVPKTIFNTKKR
ARWVSPDRGFRGDHS

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=416, Percent_Identity=52.6442307692308, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1786560, Length=229, Percent_Identity=26.2008733624454, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 48384; Mature: 48384

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLDILFAFAFLYPLLMAWTWMVGGLWFFFKREYHEQQLPEPSSEGCSIIIPCFNEVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHH
QVRQTIRYALQTKYPNFEVIAVNDGSSDSTAEILDELAAQDARLRVVHLAENQGKAVALR
HHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEEE
SGVLVSKYEYLVCIDGDALLHPHAVLWLMQPFLNFPRIGAVTGNPRILNRSSILGKLQVG
CCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEHHHHHCCCCCC
EFSSIIGLIKRAQRTYGRIFTVSGVIAAFRKTALVRVGFWSDDKITEDIDISWKLQMDHW
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEECCC
DIQYIPQALCYIYMPETFKGLWKQRLRWAQGGVEVLLEYIPKMFKLRLRRMWPVMLEALV
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIIWSYVMIMIFILFFVGLFVDLPQQFQINSLMPQWYGVILGGTCLVQFLVSLWIDHRYD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGRLFRNYFWVIWYPLFFWLLTLFTSVVAVPKTIFNTKKRARWVSPDRGFRGDHS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVLLDILFAFAFLYPLLMAWTWMVGGLWFFFKREYHEQQLPEPSSEGCSIIIPCFNEVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHH
QVRQTIRYALQTKYPNFEVIAVNDGSSDSTAEILDELAAQDARLRVVHLAENQGKAVALR
HHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEEE
SGVLVSKYEYLVCIDGDALLHPHAVLWLMQPFLNFPRIGAVTGNPRILNRSSILGKLQVG
CCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEHHHHHCCCCCC
EFSSIIGLIKRAQRTYGRIFTVSGVIAAFRKTALVRVGFWSDDKITEDIDISWKLQMDHW
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEECCC
DIQYIPQALCYIYMPETFKGLWKQRLRWAQGGVEVLLEYIPKMFKLRLRRMWPVMLEALV
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIIWSYVMIMIFILFFVGLFVDLPQQFQINSLMPQWYGVILGGTCLVQFLVSLWIDHRYD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RGRLFRNYFWVIWYPLFFWLLTLFTSVVAVPKTIFNTKKRARWVSPDRGFRGDHS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]