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The map label for this gene is pgaA [H]

Identifier: 213158546

GI number: 213158546

Start: 2591925

End: 2592932

Strand: Reverse

Name: pgaA [H]

Synonym: AB57_2498

Alternate gene names: 213158546

Gene position: 2592932-2591925 (Counterclockwise)

Preceding gene: 213158547

Following gene: 213158545

Centisome position: 64.01

GC content: 43.75

Gene sequence:

>1008_bases
ATGCAAAATGCTCAAGCTTTAGGTGATATTCCGACATGGCGAAAAACGACTCAAAACTTAGTGCAATATTACCCTGATGA
TAGTGGGGTCATTAAAAGTCGAAAAGAATTAGAAGATCGTAATAGAGCCACTATTTCACACTCAACCACTTGGGGGCAAA
GTAAGGCCGACGGTCGCGATACTGTAAGTGGGCAGAATGGTTTAAAAGACCGTGAAATGGAAACGCGTCTTAATAGCCCT
TGGATTAATGATAATTATCGTTTATTCGCTTGGCATCAAGATCGTTATGGTGAGTACCGTTTTGGCGATGTGCATGACCA
ACGTTATGGGGTCGGAGCAGAATGGCAGGCGAACCGTAAGGCGTTGTCGGCAATTGTGTCGCAAAGTACGGATGGTGGAC
AAGCAGGAGTCCGTCTTGATTGGTCCCAATGGCTAAACGACCACTGGCAGTATCAATTGCAATATAACAGTCAGGCTGAC
ATTCCTCTACAAGCCCTTGATGCAGGGGAAGATGGTCAATCTTACCGAGCTGCTGTCACATGGCAAAAAGATGAATCTCG
CCAGATTGGCGCGAGTTACGGTTTAACTGATATTAGTGATGGTAACAAACAACAGGAATTTTCAACATTTTGGCGTGAAA
GGCTATTCGACGCACCTCATCATATTACTTATGGCACTGTTCGAGGTTTCTACGGTACTAACAGCCAAGATCAAACAGCT
TATTTTAGTCCAAGTTCACATTACAGCGCTGAGTTGAACTTAAGTCATGACTGGGTGACTTGGCGTGAATATGAGCGTTC
ATTTAAGCAGCATTTTGAAGCGGGTGTAGGCCTTTATAAACAGGCCGATTACTCTGCTAAACCTACTTACTCTTTGCAAT
ATCAACATCAATGGCAGCTATCCCGGACATGGCAGCTCAATTATGGAATTGGTTGGCAATACCATCCTTACGATGGACAT
GATGAACAACATACCTACGGTATTTTTGGATTTGAAGGGCGTTTTTAA

Upstream 100 bases:

>100_bases
GCGTGTAGAACGTTGGAGAGAAAAACCGCTAGAGGCAAAAAAAACAATTTCCCGGTTAAATGGTATAGATCCGATTGCTA
AAGACACCCGCATTAATGAA

Downstream 100 bases:

>100_bases
TGAATAATTTATTGTCGAAAATGTTGGGCTGTGCCTTGGTCTCAACATTGTTGCCTCTGCAATTTGCATATGCCCAGATA
GAAAAAGATTTACCAAAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: PGA export protein; Poly-beta-1,6-GlcNAc export protein [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MQNAQALGDIPTWRKTTQNLVQYYPDDSGVIKSRKELEDRNRATISHSTTWGQSKADGRDTVSGQNGLKDREMETRLNSP
WINDNYRLFAWHQDRYGEYRFGDVHDQRYGVGAEWQANRKALSAIVSQSTDGGQAGVRLDWSQWLNDHWQYQLQYNSQAD
IPLQALDAGEDGQSYRAAVTWQKDESRQIGASYGLTDISDGNKQQEFSTFWRERLFDAPHHITYGTVRGFYGTNSQDQTA
YFSPSSHYSAELNLSHDWVTWREYERSFKQHFEAGVGLYKQADYSAKPTYSLQYQHQWQLSRTWQLNYGIGWQYHPYDGH
DEQHTYGIFGFEGRF

Sequences:

>Translated_335_residues
MQNAQALGDIPTWRKTTQNLVQYYPDDSGVIKSRKELEDRNRATISHSTTWGQSKADGRDTVSGQNGLKDREMETRLNSP
WINDNYRLFAWHQDRYGEYRFGDVHDQRYGVGAEWQANRKALSAIVSQSTDGGQAGVRLDWSQWLNDHWQYQLQYNSQAD
IPLQALDAGEDGQSYRAAVTWQKDESRQIGASYGLTDISDGNKQQEFSTFWRERLFDAPHHITYGTVRGFYGTNSQDQTA
YFSPSSHYSAELNLSHDWVTWREYERSFKQHFEAGVGLYKQADYSAKPTYSLQYQHQWQLSRTWQLNYGIGWQYHPYDGH
DEQHTYGIFGFEGRF
>Mature_335_residues
MQNAQALGDIPTWRKTTQNLVQYYPDDSGVIKSRKELEDRNRATISHSTTWGQSKADGRDTVSGQNGLKDREMETRLNSP
WINDNYRLFAWHQDRYGEYRFGDVHDQRYGVGAEWQANRKALSAIVSQSTDGGQAGVRLDWSQWLNDHWQYQLQYNSQAD
IPLQALDAGEDGQSYRAAVTWQKDESRQIGASYGLTDISDGNKQQEFSTFWRERLFDAPHHITYGTVRGFYGTNSQDQTA
YFSPSSHYSAELNLSHDWVTWREYERSFKQHFEAGVGLYKQADYSAKPTYSLQYQHQWQLSRTWQLNYGIGWQYHPYDGH
DEQHTYGIFGFEGRF

Specific function: Exports the biofilm adhesin polysaccharide poly-beta- 1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N- deacetylation of PGA by PgaB is needed for PGA export through the PgaA pori

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI1787261, Length=325, Percent_Identity=28.9230769230769, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39073; Mature: 39073

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNAQALGDIPTWRKTTQNLVQYYPDDSGVIKSRKELEDRNRATISHSTTWGQSKADGRD
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
TVSGQNGLKDREMETRLNSPWINDNYRLFAWHQDRYGEYRFGDVHDQRYGVGAEWQANRK
CCCCCCCCCCHHHHHHCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHH
ALSAIVSQSTDGGQAGVRLDWSQWLNDHWQYQLQYNSQADIPLQALDAGEDGQSYRAAVT
HHHHHHHCCCCCCCCCEEECHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCCEEEEEE
WQKDESRQIGASYGLTDISDGNKQQEFSTFWRERLFDAPHHITYGTVRGFYGTNSQDQTA
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEE
YFSPSSHYSAELNLSHDWVTWREYERSFKQHFEAGVGLYKQADYSAKPTYSLQYQHQWQL
EECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEEEEEEE
SRTWQLNYGIGWQYHPYDGHDEQHTYGIFGFEGRF
EEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure
MQNAQALGDIPTWRKTTQNLVQYYPDDSGVIKSRKELEDRNRATISHSTTWGQSKADGRD
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
TVSGQNGLKDREMETRLNSPWINDNYRLFAWHQDRYGEYRFGDVHDQRYGVGAEWQANRK
CCCCCCCCCCHHHHHHCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHH
ALSAIVSQSTDGGQAGVRLDWSQWLNDHWQYQLQYNSQADIPLQALDAGEDGQSYRAAVT
HHHHHHHCCCCCCCCCEEECHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCCEEEEEE
WQKDESRQIGASYGLTDISDGNKQQEFSTFWRERLFDAPHHITYGTVRGFYGTNSQDQTA
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCEE
YFSPSSHYSAELNLSHDWVTWREYERSFKQHFEAGVGLYKQADYSAKPTYSLQYQHQWQL
EECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEEEEEEE
SRTWQLNYGIGWQYHPYDGHDEQHTYGIFGFEGRF
EEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]