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The map label for this gene is miaE [H]

Identifier: 213158237

GI number: 213158237

Start: 3053761

End: 3054405

Strand: Reverse

Name: miaE [H]

Synonym: AB57_2956

Alternate gene names: 213158237

Gene position: 3054405-3053761 (Counterclockwise)

Preceding gene: 213158238

Following gene: 213158236

Centisome position: 75.41

GC content: 40.78

Gene sequence:

>645_bases
ATGTCGAGTATCAATTACGATGAATTAATGCAGCCTGTATTAGCATTTTTAGGGTGCCAAACCCCAAAAGAATGGTTGGA
TGAGGCCGTTAATAATTTAGACATCTTGATGCAAGATCATGCTAACTGTGAGAAAAAGGCAGCGGGTACGGCCATGAACT
TGATGTTCCGCTATAGTTTTTTTACAGACCTACAAGTTAAGCTTGCCCAGCTTGTACGTGAAGAAATGCTTCACTATGAA
CAAGTTCTAGAATTTATGACGAAACGTGGGCAAGAGTGGAAAGGTTTAAGTGCTGGACGCTATGCAGGTGGCTTACGAAA
AGAAATCCGCACATATGAACCAGAAGCTCTAATTGATGTACTCGTGATTGGGGCTTTTGTAGAAGCACGTTCATGTGAAC
GCTTTTATGCGCTTGCTCCGCGTGTTGATGATGAGTTAGGACGCTATTACCGTTACTTGCTTAAGTCAGAATCTCGTCAT
TTTGAAGACTATTTAGCACTAGCGCTTGATGTTGCAAAAACTGCCAAAATGAAAGATCCTGAAGAAGATATTCAACAGCG
TATTGAACATATCCGTGAAGTTGAAAAGAATCTGATTTTGACTCCAGATGATACTTTCCGTTTTCATAGTGGTATCCCTG
CATAA

Upstream 100 bases:

>100_bases
AAATATTGGTCACTCAATTATTGCGGAAAGTGTTTTTGTTGGACTGGTACAGGCGGTGAAAGACATGAAAACAGCGATTC
AGGCAGCAGGATAAGTAGTA

Downstream 100 bases:

>100_bases
GCTTGGATACCTCAAGTTAAAAAGTTTACCTTTCACGGTAAACTTTTTTATTAAGTAGCTCTAACTTCTAAATAGTTTGA
GCCGATATTTAAGGTTAAGT

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSSINYDELMQPVLAFLGCQTPKEWLDEAVNNLDILMQDHANCEKKAAGTAMNLMFRYSFFTDLQVKLAQLVREEMLHYE
QVLEFMTKRGQEWKGLSAGRYAGGLRKEIRTYEPEALIDVLVIGAFVEARSCERFYALAPRVDDELGRYYRYLLKSESRH
FEDYLALALDVAKTAKMKDPEEDIQQRIEHIREVEKNLILTPDDTFRFHSGIPA

Sequences:

>Translated_214_residues
MSSINYDELMQPVLAFLGCQTPKEWLDEAVNNLDILMQDHANCEKKAAGTAMNLMFRYSFFTDLQVKLAQLVREEMLHYE
QVLEFMTKRGQEWKGLSAGRYAGGLRKEIRTYEPEALIDVLVIGAFVEARSCERFYALAPRVDDELGRYYRYLLKSESRH
FEDYLALALDVAKTAKMKDPEEDIQQRIEHIREVEKNLILTPDDTFRFHSGIPA
>Mature_213_residues
SSINYDELMQPVLAFLGCQTPKEWLDEAVNNLDILMQDHANCEKKAAGTAMNLMFRYSFFTDLQVKLAQLVREEMLHYEQ
VLEFMTKRGQEWKGLSAGRYAGGLRKEIRTYEPEALIDVLVIGAFVEARSCERFYALAPRVDDELGRYYRYLLKSESRHF
EDYLALALDVAKTAKMKDPEEDIQQRIEHIREVEKNLILTPDDTFRFHSGIPA

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 24867; Mature: 24736

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSINYDELMQPVLAFLGCQTPKEWLDEAVNNLDILMQDHANCEKKAAGTAMNLMFRYSF
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FTDLQVKLAQLVREEMLHYEQVLEFMTKRGQEWKGLSAGRYAGGLRKEIRTYEPEALIDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
LVIGAFVEARSCERFYALAPRVDDELGRYYRYLLKSESRHFEDYLALALDVAKTAKMKDP
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCC
EEDIQQRIEHIREVEKNLILTPDDTFRFHSGIPA
HHHHHHHHHHHHHHHHCEECCCCCCHHCCCCCCC
>Mature Secondary Structure 
SSINYDELMQPVLAFLGCQTPKEWLDEAVNNLDILMQDHANCEKKAAGTAMNLMFRYSF
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FTDLQVKLAQLVREEMLHYEQVLEFMTKRGQEWKGLSAGRYAGGLRKEIRTYEPEALIDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
LVIGAFVEARSCERFYALAPRVDDELGRYYRYLLKSESRHFEDYLALALDVAKTAKMKDP
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCC
EEDIQQRIEHIREVEKNLILTPDDTFRFHSGIPA
HHHHHHHHHHHHHHHHCEECCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]