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The map label for this gene is azoR [H]

Identifier: 213157256

GI number: 213157256

Start: 2030746

End: 2031354

Strand: Direct

Name: azoR [H]

Synonym: AB57_1943

Alternate gene names: 213157256

Gene position: 2030746-2031354 (Clockwise)

Preceding gene: 213157255

Following gene: 213157258

Centisome position: 50.14

GC content: 42.69

Gene sequence:

>609_bases
ATGAAACTACTTCAAATTGACACAAGTATTTTAGGTGAGCAGTCTGTTAGCCGCCAACTCACCTCATCTGTTATTCGTCA
GCTCAGCACAGCTTATCCAGATGCAGAAATTATTCATCACGATTTTGCTTTAGAGCCAATTCCTCATTTGTCTGATGCTG
AGTTTTTAGCATGGCAAGGTGTTGAACCTAATAATGAAACAGCGCAACAACGTGTTGCAAGAAACACCCAATATTTGGAT
GAGTTCTTATCGTCAGATATCGTTGTAATTGGCGCACCTATGTATAACTTTAGCTTCCCATCACAGTTAAAAGCGTGGCT
TGACCGTCTATCTGTTGCTGGTAAAACATTCCGTTACACGGAAAACGGTCCTCAAGGTTTAGTAGAGGGGAAACGTGTCA
TTATTGCTTCTTCGCGTGGTGGTGTGTATTCAGAAGGTTCACCAGCCGAAGCTTTAGACTATCAAGAAACTTATATCAAA
GCGTTTTTCAATTTTATTGGGGTGACTGATATCACTTTTGTGCGCGCCGAAGGAATCGCTTTCGGACCAGAAGCACGTCA
AGCTGCCTTAGATAATGCAGCAGCGCAAATTGCCCAGTTAGCGGGTTAA

Upstream 100 bases:

>100_bases
GTCATATTACTTGGTTCCCTAAATGTGCCTTCTTGTGCATAAGAATAATAGTAGTAACATAATTAGCTCAAGTAACAATT
TTATACTGTAGGTATAAGCG

Downstream 100 bases:

>100_bases
AAATATAAATAGCCGAACAGTAAAGGAACTGTTCGGCCTTATTAAGATTATGATTTTAAGAAATTAATTCAATCAGTAAA
TAACTAAAAGAAAGTGACAG

Product: fmn-dependent NADH-azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MKLLQIDTSILGEQSVSRQLTSSVIRQLSTAYPDAEIIHHDFALEPIPHLSDAEFLAWQGVEPNNETAQQRVARNTQYLD
EFLSSDIVVIGAPMYNFSFPSQLKAWLDRLSVAGKTFRYTENGPQGLVEGKRVIIASSRGGVYSEGSPAEALDYQETYIK
AFFNFIGVTDITFVRAEGIAFGPEARQAALDNAAAQIAQLAG

Sequences:

>Translated_202_residues
MKLLQIDTSILGEQSVSRQLTSSVIRQLSTAYPDAEIIHHDFALEPIPHLSDAEFLAWQGVEPNNETAQQRVARNTQYLD
EFLSSDIVVIGAPMYNFSFPSQLKAWLDRLSVAGKTFRYTENGPQGLVEGKRVIIASSRGGVYSEGSPAEALDYQETYIK
AFFNFIGVTDITFVRAEGIAFGPEARQAALDNAAAQIAQLAG
>Mature_202_residues
MKLLQIDTSILGEQSVSRQLTSSVIRQLSTAYPDAEIIHHDFALEPIPHLSDAEFLAWQGVEPNNETAQQRVARNTQYLD
EFLSSDIVVIGAPMYNFSFPSQLKAWLDRLSVAGKTFRYTENGPQGLVEGKRVIIASSRGGVYSEGSPAEALDYQETYIK
AFFNFIGVTDITFVRAEGIAFGPEARQAALDNAAAQIAQLAG

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=198, Percent_Identity=40.9090909090909, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 22173; Mature: 22173

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLQIDTSILGEQSVSRQLTSSVIRQLSTAYPDAEIIHHDFALEPIPHLSDAEFLAWQG
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEECC
VEPNNETAQQRVARNTQYLDEFLSSDIVVIGAPMYNFSFPSQLKAWLDRLSVAGKTFRYT
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEC
ENGPQGLVEGKRVIIASSRGGVYSEGSPAEALDYQETYIKAFFNFIGVTDITFVRAEGIA
CCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCE
FGPEARQAALDNAAAQIAQLAG
ECCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLLQIDTSILGEQSVSRQLTSSVIRQLSTAYPDAEIIHHDFALEPIPHLSDAEFLAWQG
CCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEECC
VEPNNETAQQRVARNTQYLDEFLSSDIVVIGAPMYNFSFPSQLKAWLDRLSVAGKTFRYT
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEC
ENGPQGLVEGKRVIIASSRGGVYSEGSPAEALDYQETYIKAFFNFIGVTDITFVRAEGIA
CCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHEEEEECCCE
FGPEARQAALDNAAAQIAQLAG
ECCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]