Click here to switch to the map view.

The map label for this gene is serA [H]

Identifier: 212224614

GI number: 212224614

Start: 1341022

End: 1341945

Strand: Reverse

Name: serA [H]

Synonym: TON_1463

Alternate gene names: 212224614

Gene position: 1341945-1341022 (Counterclockwise)

Preceding gene: 212224615

Following gene: 212224613

Centisome position: 72.63

GC content: 55.74

Gene sequence:

>924_bases
ATGAATGTGGTCAAGGTTTTGGTTGCCGCACCGCTTCACGAGAAGGCAATAGAGGTTTTGAAGAACGCTGGGTTTGAGGT
AGTTTGTGAGGAGTATCCAGATGAGGATAAACTGATAGAGCTTGCCAAGGATGTTGAGGCGATTATAGTCAGGAGCAAGC
CTAAGGTGACCCGGAAGGTTATTGAGGCGGCTCCAAAGCTCAAGGTTATCGGAAGGGCTGGCGTCGGCCTCGACAACATC
GACCTTGATGCCGCCAAGGAGAGGGGCATAAAGGTCGTTAACAGCCCTGGAGCATCAAGCAGGAGCGTCGCTGAGCTTGT
CGTCGCCCTCATGTTTGCCGTTGCCAGAAAGATAGCCTTCGCCGACAGGAAGATGCGCGGGGGCGTTTGGGCCAAGAAGC
AGTGTATGGGGATCGAGCTCGAAGGAAAGACCATAGGCGTAGTCGGTTTCGGCAGAATAGGCTACAACGTGGCCAAGCTA
GCAAAGGCTCTTGGCATGAACGTCCTCCTCTACGACCCATATCCAGATGAGGAAAGGGCCAAAGAAGTTGGAGGAAAGTT
CGTCAGCCTGGAGGAGCTTCTCAAGGAGAGCGATGTTGTTACGCTTCACGTCCCGCTTATCGATGCCACCTACCACATGA
TAAACGAGGAGCGGCTGAAGCTGATGAAGCCGACCGCCATCCTTATCAACGCGGCCAGGGGAGCCGTTATAGACACCGAA
GCCCTTGTCAAGGCCCTCGGGGAGGGCTGGATTGCTGGAGCTGGCTTGGACGTCTTCGAGGAGGAGCCCCTGCCTGAGGG
TCATCCGCTTACGAAGTTCGACAACGTTGTCCTCACCCCGCACATCGGCGCCTCGACCGTCGAGGCCCAGATGAGGGCCG
GCGTCCAGGTCGCCGAGCAGATAGTTGAGATTCTAAAGAAGTGA

Upstream 100 bases:

>100_bases
AACTTGTATGGAACCAGATACTGACGAAAATTGGAGACATAGCCGACCGCGCTGAGGACACCTCCGATCAGGTCATGCTC
ATGGCAATAAAGAGGAGGGG

Downstream 100 bases:

>100_bases
TGGCCTTTCCTTCTACCGTCTATTTTTCATCTACGTTGTGTCTATGGAATGCCCGAAATATAACCAAAACTTTTTAAAGC
TGGGTCAGGTTCACTATAAC

Product: D-3-phosphoglycerate dehydrogenase

Products: NA

Alternate protein names: PGDH [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNVVKVLVAAPLHEKAIEVLKNAGFEVVCEEYPDEDKLIELAKDVEAIIVRSKPKVTRKVIEAAPKLKVIGRAGVGLDNI
DLDAAKERGIKVVNSPGASSRSVAELVVALMFAVARKIAFADRKMRGGVWAKKQCMGIELEGKTIGVVGFGRIGYNVAKL
AKALGMNVLLYDPYPDEERAKEVGGKFVSLEELLKESDVVTLHVPLIDATYHMINEERLKLMKPTAILINAARGAVIDTE
ALVKALGEGWIAGAGLDVFEEEPLPEGHPLTKFDNVVLTPHIGASTVEAQMRAGVQVAEQIVEILKK

Sequences:

>Translated_307_residues
MNVVKVLVAAPLHEKAIEVLKNAGFEVVCEEYPDEDKLIELAKDVEAIIVRSKPKVTRKVIEAAPKLKVIGRAGVGLDNI
DLDAAKERGIKVVNSPGASSRSVAELVVALMFAVARKIAFADRKMRGGVWAKKQCMGIELEGKTIGVVGFGRIGYNVAKL
AKALGMNVLLYDPYPDEERAKEVGGKFVSLEELLKESDVVTLHVPLIDATYHMINEERLKLMKPTAILINAARGAVIDTE
ALVKALGEGWIAGAGLDVFEEEPLPEGHPLTKFDNVVLTPHIGASTVEAQMRAGVQVAEQIVEILKK
>Mature_307_residues
MNVVKVLVAAPLHEKAIEVLKNAGFEVVCEEYPDEDKLIELAKDVEAIIVRSKPKVTRKVIEAAPKLKVIGRAGVGLDNI
DLDAAKERGIKVVNSPGASSRSVAELVVALMFAVARKIAFADRKMRGGVWAKKQCMGIELEGKTIGVVGFGRIGYNVAKL
AKALGMNVLLYDPYPDEERAKEVGGKFVSLEELLKESDVVTLHVPLIDATYHMINEERLKLMKPTAILINAARGAVIDTE
ALVKALGEGWIAGAGLDVFEEEPLPEGHPLTKFDNVVLTPHIGASTVEAQMRAGVQVAEQIVEILKK

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=305, Percent_Identity=41.9672131147541, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI6912396, Length=258, Percent_Identity=36.046511627907, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI61743967, Length=294, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI4557497, Length=294, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI145580578, Length=245, Percent_Identity=37.1428571428571, Blast_Score=157, Evalue=8e-39,
Organism=Homo sapiens, GI4557499, Length=245, Percent_Identity=37.1428571428571, Blast_Score=157, Evalue=8e-39,
Organism=Homo sapiens, GI145580575, Length=255, Percent_Identity=36.8627450980392, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI1789279, Length=313, Percent_Identity=40.5750798722045, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI87082289, Length=260, Percent_Identity=36.5384615384615, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1787645, Length=247, Percent_Identity=36.0323886639676, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788660, Length=260, Percent_Identity=35.3846153846154, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081824, Length=154, Percent_Identity=33.1168831168831, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=303, Percent_Identity=40.9240924092409, Blast_Score=228, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI25147481, Length=278, Percent_Identity=28.7769784172662, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=310, Percent_Identity=39.3548387096774, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6322116, Length=308, Percent_Identity=38.3116883116883, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324055, Length=228, Percent_Identity=36.8421052631579, Blast_Score=148, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6324964, Length=277, Percent_Identity=33.9350180505415, Blast_Score=132, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6324980, Length=183, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6325144, Length=305, Percent_Identity=25.2459016393443, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321253, Length=271, Percent_Identity=26.9372693726937, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921140, Length=307, Percent_Identity=40.7166123778502, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24646446, Length=261, Percent_Identity=37.5478927203065, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24646448, Length=261, Percent_Identity=37.5478927203065, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24646452, Length=261, Percent_Identity=37.5478927203065, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24646450, Length=261, Percent_Identity=37.5478927203065, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI62472511, Length=261, Percent_Identity=37.5478927203065, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24585516, Length=309, Percent_Identity=29.7734627831715, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI28574286, Length=308, Percent_Identity=30.8441558441558, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28571528, Length=311, Percent_Identity=34.4051446945338, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45552429, Length=290, Percent_Identity=31.3793103448276, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24585514, Length=290, Percent_Identity=31.3793103448276, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI28574282, Length=290, Percent_Identity=31.3793103448276, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45551003, Length=290, Percent_Identity=31.3793103448276, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28574284, Length=290, Percent_Identity=31.3793103448276, Blast_Score=131, Evalue=6e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR006236
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C; PF01842 ACT [H]

EC number: =1.1.1.95 [H]

Molecular weight: Translated: 33214; Mature: 33214

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVKVLVAAPLHEKAIEVLKNAGFEVVCEEYPDEDKLIELAKDVEAIIVRSKPKVTRKV
CCHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHEEECCCCHHHHHH
IEAAPKLKVIGRAGVGLDNIDLDAAKERGIKVVNSPGASSRSVAELVVALMFAVARKIAF
HHHCCCEEEEECCCCCCCCCCCCHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ADRKMRGGVWAKKQCMGIELEGKTIGVVGFGRIGYNVAKLAKALGMNVLLYDPYPDEERA
HHHHHCCCCCHHHHEEEEEECCCEEEEEEECHHHHHHHHHHHHHCCEEEEECCCCCHHHH
KEVGGKFVSLEELLKESDVVTLHVPLIDATYHMINEERLKLMKPTAILINAARGAVIDTE
HHHCCCEECHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECHH
ALVKALGEGWIAGAGLDVFEEEPLPEGHPLTKFDNVVLTPHIGASTVEAQMRAGVQVAEQ
HHHHHHCCCCEECCCCCHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
IVEILKK
HHHHHCC
>Mature Secondary Structure
MNVVKVLVAAPLHEKAIEVLKNAGFEVVCEEYPDEDKLIELAKDVEAIIVRSKPKVTRKV
CCHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHEEECCCCHHHHHH
IEAAPKLKVIGRAGVGLDNIDLDAAKERGIKVVNSPGASSRSVAELVVALMFAVARKIAF
HHHCCCEEEEECCCCCCCCCCCCHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
ADRKMRGGVWAKKQCMGIELEGKTIGVVGFGRIGYNVAKLAKALGMNVLLYDPYPDEERA
HHHHHCCCCCHHHHEEEEEECCCEEEEEEECHHHHHHHHHHHHHCCEEEEECCCCCHHHH
KEVGGKFVSLEELLKESDVVTLHVPLIDATYHMINEERLKLMKPTAILINAARGAVIDTE
HHHCCCEECHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECHH
ALVKALGEGWIAGAGLDVFEEEPLPEGHPLTKFDNVVLTPHIGASTVEAQMRAGVQVAEQ
HHHHHHCCCCEECCCCCHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
IVEILKK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]