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The map label for this gene is dapE [H]

Identifier: 212224613

GI number: 212224613

Start: 1339388

End: 1340494

Strand: Reverse

Name: dapE [H]

Synonym: TON_1462

Alternate gene names: 212224613

Gene position: 1340494-1339388 (Counterclockwise)

Preceding gene: 212224614

Following gene: 212224612

Centisome position: 72.55

GC content: 55.28

Gene sequence:

>1107_bases
ATGGAGTTCGAACTCCTCAAGAAGCTCGTCTCCATTCGCTCTCCCTTTGGCGAAGAAGGAGAAATCTCCCGTTTTATTGC
CTCGTTTCTTGAAGAGCACGGCTTTAGGGTGGAGCTCCTCCCGGTTGAAGGCTTTGGCGACGACGTGATTGTCTATCTCC
CTGGTAAGGGCCACACCGTTGTTCTGAACGGCCACATGGACACCGTGAATCTCTCGAAGGGCTGGACAAGGAATCCCTGG
GGCGAGCTCGATGGCGATAGATTTTACGGCCTCGGCAGTGCGGACATGAAAGCAGGTCTTGCCGCTATCATGGCTGCCTT
CATTGAGATAGCTGAGCTTCCGAGAAGAGAGCGGCCGAACATCATATTCACCGCAGTCTCCGACGAGGAGGGCTATTCCC
GGGGAACCTGGAGGCTCATAGAGAGTGGAAAACTTAGTGATGCCGACATCGTGCTCGTTGCCGAGCCCACTAACGAGAAG
CTGATGCTCGGCGCGAGGGGACGATTCGTGATCCAAGTCGAGGCCTTTGGAAGAAAGGCCCACGCAGCCCGGCCGGAGTT
TGGAATCAATGCCGTTGAGGAGCTTGGGAAACTCGTTGGAAACCTCTCAAGGATACGTTTTAAGAGGCACAGGAAGCTCG
GCACGGGCTCATACTGCACGCTCCACATCGAAGGAACCGCCGACGGCCTGAGCGTTCCCGACTATGCGAAGGCGATAATA
GACAGGCACGTTGTCGTTGGTGAGGACTGGGAAAAAGTCAGGGGCGAGCTCCTAAAACTTGCTGAGAGGCTCGATCTGAA
GGTCGAACTCAAAATAGAGAGGTTCCCGAGGCCAACGCCCGAGATGCTGCCCTACACTGTAAGGGAAAACGACCGCTTTG
TGAACCTTTTCAAAAAGGTCCGCGGCTCGCTCTTCGGAAGGGGGCTGGAGATAACTTACGGCAGGAGCGTCGGGGACTTC
AACTACTTCGGAACATATCTGGGCAAGCCAACAATCGTTTACGGGCCCATCGGAGGGAACTGGCACTCCGCCGATGAGTG
GGTGAGTGTAGAGTCACTGAGGAGGGTTAAGAAGGTCTACGTAGAGTACCTGAAGGCTCTGGCATAG

Upstream 100 bases:

>100_bases
CCTTTGATCAGACTTTGTCCCACAAAGTTTGCCTGCACAAGCAAAAGTGTTTGGGAAAGGGCTTTAAGATGCTTGCCATT
TCTAGTCTTTGGTGATCCTC

Downstream 100 bases:

>100_bases
CCTCAGGAAACCGGTCTTCATCACGGCTCAGTCCAGGGAGTTCTCGTCATCGGCGGCAAAGGTTTTAAGCTTCCCCTAAA
ATCTTTTTCTATGTTCCACG

Product: ArgE/DapE-related deacylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MEFELLKKLVSIRSPFGEEGEISRFIASFLEEHGFRVELLPVEGFGDDVIVYLPGKGHTVVLNGHMDTVNLSKGWTRNPW
GELDGDRFYGLGSADMKAGLAAIMAAFIEIAELPRRERPNIIFTAVSDEEGYSRGTWRLIESGKLSDADIVLVAEPTNEK
LMLGARGRFVIQVEAFGRKAHAARPEFGINAVEELGKLVGNLSRIRFKRHRKLGTGSYCTLHIEGTADGLSVPDYAKAII
DRHVVVGEDWEKVRGELLKLAERLDLKVELKIERFPRPTPEMLPYTVRENDRFVNLFKKVRGSLFGRGLEITYGRSVGDF
NYFGTYLGKPTIVYGPIGGNWHSADEWVSVESLRRVKKVYVEYLKALA

Sequences:

>Translated_368_residues
MEFELLKKLVSIRSPFGEEGEISRFIASFLEEHGFRVELLPVEGFGDDVIVYLPGKGHTVVLNGHMDTVNLSKGWTRNPW
GELDGDRFYGLGSADMKAGLAAIMAAFIEIAELPRRERPNIIFTAVSDEEGYSRGTWRLIESGKLSDADIVLVAEPTNEK
LMLGARGRFVIQVEAFGRKAHAARPEFGINAVEELGKLVGNLSRIRFKRHRKLGTGSYCTLHIEGTADGLSVPDYAKAII
DRHVVVGEDWEKVRGELLKLAERLDLKVELKIERFPRPTPEMLPYTVRENDRFVNLFKKVRGSLFGRGLEITYGRSVGDF
NYFGTYLGKPTIVYGPIGGNWHSADEWVSVESLRRVKKVYVEYLKALA
>Mature_368_residues
MEFELLKKLVSIRSPFGEEGEISRFIASFLEEHGFRVELLPVEGFGDDVIVYLPGKGHTVVLNGHMDTVNLSKGWTRNPW
GELDGDRFYGLGSADMKAGLAAIMAAFIEIAELPRRERPNIIFTAVSDEEGYSRGTWRLIESGKLSDADIVLVAEPTNEK
LMLGARGRFVIQVEAFGRKAHAARPEFGINAVEELGKLVGNLSRIRFKRHRKLGTGSYCTLHIEGTADGLSVPDYAKAII
DRHVVVGEDWEKVRGELLKLAERLDLKVELKIERFPRPTPEMLPYTVRENDRFVNLFKKVRGSLFGRGLEITYGRSVGDF
NYFGTYLGKPTIVYGPIGGNWHSADEWVSVESLRRVKKVYVEYLKALA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789236, Length=349, Percent_Identity=25.7879656160458, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790395, Length=361, Percent_Identity=24.3767313019391, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41362; Mature: 41362

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFELLKKLVSIRSPFGEEGEISRFIASFLEEHGFRVELLPVEGFGDDVIVYLPGKGHTV
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCEE
VLNGHMDTVNLSKGWTRNPWGELDGDRFYGLGSADMKAGLAAIMAAFIEIAELPRRERPN
EEECCCCEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IIFTAVSDEEGYSRGTWRLIESGKLSDADIVLVAEPTNEKLMLGARGRFVIQVEAFGRKA
EEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEECCCEEEEEEEECCCHH
HAARPEFGINAVEELGKLVGNLSRIRFKRHRKLGTGSYCTLHIEGTADGLSVPDYAKAII
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHH
DRHVVVGEDWEKVRGELLKLAERLDLKVELKIERFPRPTPEMLPYTVRENDRFVNLFKKV
HCCEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHCCEEECCCCHHHHHHHHH
RGSLFGRGLEITYGRSVGDFNYFGTYLGKPTIVYGPIGGNWHSADEWVSVESLRRVKKVY
HHHHHCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHH
VEYLKALA
HHHHHHHC
>Mature Secondary Structure
MEFELLKKLVSIRSPFGEEGEISRFIASFLEEHGFRVELLPVEGFGDDVIVYLPGKGHTV
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCEE
VLNGHMDTVNLSKGWTRNPWGELDGDRFYGLGSADMKAGLAAIMAAFIEIAELPRRERPN
EEECCCCEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IIFTAVSDEEGYSRGTWRLIESGKLSDADIVLVAEPTNEKLMLGARGRFVIQVEAFGRKA
EEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEECCCEEEEEEEECCCHH
HAARPEFGINAVEELGKLVGNLSRIRFKRHRKLGTGSYCTLHIEGTADGLSVPDYAKAII
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHH
DRHVVVGEDWEKVRGELLKLAERLDLKVELKIERFPRPTPEMLPYTVRENDRFVNLFKKV
HCCEEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHCCEEECCCCHHHHHHHHH
RGSLFGRGLEITYGRSVGDFNYFGTYLGKPTIVYGPIGGNWHSADEWVSVESLRRVKKVY
HHHHHCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHH
VEYLKALA
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA