Click here to switch to the map view.

The map label for this gene is pcm

Identifier: 212224325

GI number: 212224325

Start: 1072706

End: 1073368

Strand: Reverse

Name: pcm

Synonym: TON_1176

Alternate gene names: 212224325

Gene position: 1073368-1072706 (Counterclockwise)

Preceding gene: 212224326

Following gene: 212224324

Centisome position: 58.1

GC content: 53.7

Gene sequence:

>663_bases
ATGTTGATGGAGGATGCTGATGTTCTTTGGTATCATACGGTGGAGAAACTTAAGAGGGAAGGCATAATCCGGAGCGAAAA
GGTCAGGCAGGCTTTTCTGAAGGTCCCGAGGTATCTCTTCGTTCTTCCTGAGCATAAAAAGTGGGCGCACGTTGACGAAC
CGCTTCCCATTCCCGGCGGTCAGACGATAAGCGCCCCCCACATGGTCGCGATAATGCTCGAGCTTGCCGAACTTGAGGAA
GGTATGAACGTTCTTGATATAGGTACCGGAAGCGGCTGGAACGCGGCTTTAGCAGCGGAGCTTGTTAAGACCGATGTCTA
TACCGTCGAGAGGATTCCGGAGCTTGTCGAGTTCGCGAGGAAAAACCTTGAAAAAGCTGGCTATGCCGATAGGGTTCACG
TCATCATCGGCGATGGCACCAAAGGTTTCCCACCTAAAGCGCCCTACGACAGGATACTGGTCGCTGCTGGAGCACCAAAT
GTCCCAGAACCTCTCGTTGAACAGCTCAAGCCTGGAGGGAAGCTCATAATCCCCGTCGGGAGCTACCACCTTTGGCAGGA
GTTGTACGAGGTAATAAAGCTTAAGGACGGAAGCGTGAAGGTCAAGCGGCACGGTGGAGTTGCCTTTGTGCCCCTCATCG
GGGAGCACGGCTGGAAGGAGTAG

Upstream 100 bases:

>100_bases
GGTGCCCTTGTCATCATAGCCCTTGCTTGGTACCAAGCCAAGAAAGTCTGAAGAGGAAGGGCAAGGTTGATACGCCCTTT
CTCATATTTTCTCTCCGGTG

Downstream 100 bases:

>100_bases
GCTTTTAAGTCGTGATGAGTTTTTCCTCTGGTGGTATCATGGTGAGACTGATTGCCTTTGACCTTGAGGGAACGCTGGTC
AAATCCGTCTCAAGCTGGGT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MLMEDADVLWYHTVEKLKREGIIRSEKVRQAFLKVPRYLFVLPEHKKWAHVDEPLPIPGGQTISAPHMVAIMLELAELEE
GMNVLDIGTGSGWNAALAAELVKTDVYTVERIPELVEFARKNLEKAGYADRVHVIIGDGTKGFPPKAPYDRILVAAGAPN
VPEPLVEQLKPGGKLIIPVGSYHLWQELYEVIKLKDGSVKVKRHGGVAFVPLIGEHGWKE

Sequences:

>Translated_220_residues
MLMEDADVLWYHTVEKLKREGIIRSEKVRQAFLKVPRYLFVLPEHKKWAHVDEPLPIPGGQTISAPHMVAIMLELAELEE
GMNVLDIGTGSGWNAALAAELVKTDVYTVERIPELVEFARKNLEKAGYADRVHVIIGDGTKGFPPKAPYDRILVAAGAPN
VPEPLVEQLKPGGKLIIPVGSYHLWQELYEVIKLKDGSVKVKRHGGVAFVPLIGEHGWKE
>Mature_220_residues
MLMEDADVLWYHTVEKLKREGIIRSEKVRQAFLKVPRYLFVLPEHKKWAHVDEPLPIPGGQTISAPHMVAIMLELAELEE
GMNVLDIGTGSGWNAALAAELVKTDVYTVERIPELVEFARKNLEKAGYADRVHVIIGDGTKGFPPKAPYDRILVAAGAPN
VPEPLVEQLKPGGKLIIPVGSYHLWQELYEVIKLKDGSVKVKRHGGVAFVPLIGEHGWKE

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=207, Percent_Identity=41.0628019323672, Blast_Score=134, Evalue=5e-32,
Organism=Escherichia coli, GI1789100, Length=202, Percent_Identity=40.0990099009901, Blast_Score=119, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71983477, Length=215, Percent_Identity=33.4883720930233, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI193207222, Length=213, Percent_Identity=31.4553990610329, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17981723, Length=213, Percent_Identity=37.5586854460094, Blast_Score=123, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_THEON (B6YX51)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002307561.1
- ProteinModelPortal:   B6YX51
- GeneID:   7018198
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_1176
- HOGENOM:   HBG699907
- OMA:   VAMSKAN
- ProtClustDB:   PRK00312
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 24493; Mature: 24493

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 64-64

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMEDADVLWYHTVEKLKREGIIRSEKVRQAFLKVPRYLFVLPEHKKWAHVDEPLPIPGG
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
QTISAPHMVAIMLELAELEEGMNVLDIGTGSGWNAALAAELVKTDVYTVERIPELVEFAR
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KNLEKAGYADRVHVIIGDGTKGFPPKAPYDRILVAAGAPNVPEPLVEQLKPGGKLIIPVG
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECC
SYHLWQELYEVIKLKDGSVKVKRHGGVAFVPLIGEHGWKE
CHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCC
>Mature Secondary Structure
MLMEDADVLWYHTVEKLKREGIIRSEKVRQAFLKVPRYLFVLPEHKKWAHVDEPLPIPGG
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
QTISAPHMVAIMLELAELEEGMNVLDIGTGSGWNAALAAELVKTDVYTVERIPELVEFAR
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KNLEKAGYADRVHVIIGDGTKGFPPKAPYDRILVAAGAPNVPEPLVEQLKPGGKLIIPVG
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECC
SYHLWQELYEVIKLKDGSVKVKRHGGVAFVPLIGEHGWKE
CHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA