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The map label for this gene is thiI [H]

Identifier: 212224155

GI number: 212224155

Start: 930299

End: 930889

Strand: Reverse

Name: thiI [H]

Synonym: TON_1006

Alternate gene names: 212224155

Gene position: 930889-930299 (Counterclockwise)

Preceding gene: 212224156

Following gene: 212224153

Centisome position: 50.38

GC content: 52.96

Gene sequence:

>591_bases
ATGAAAGCTGTTGCACTGCTCAGCTCGGGCATTGATTCTCCAGTGGCGATATACCTCATGCTCAGAAAGGGCATGGAGAT
AACGCCGGTCCACTTCAAGCAGAGCGATAAGAAGGAGTCAAAGGTTCTGGAGCTCTGCGAGATTCTGAGTAAGTACGGGA
AGCTCAACGAGCCCGTAATCGTTGATGCTTACGAGGAACAGGCGCCAGTCTTTTCCAAGCTGGTCGAGATAGGAAAGGCC
AAGTGGACGTGTCTTTTCTGCAAGTACACGATGCTGAGGAAGGCCTGCAGGGTGGGGCACGAAATCGGGGCAAAAGCTAT
AGTTACGGGTGACTCCCTCGGGCAGGTGGCATCGCAGACCCTCGACAACCTGCTGATAATAAGTCAGGCGAGTGATCTGC
CTATTTTGAGGCCCCTCATCGGCCGCGACAAGGAGGAGATAGTCAAAATAGCAAAGGAGATAGGCACGTTCGAAATAAGC
ATAGAACCTGAAGAGCCCTGTCCGTTCGTGCCGAAGTATCCGGTGGTGCGCGGCTCCCTTGGGGAGTTCGAGAAGATAAG
GGAGAAACTCGTAAAGGAGGGGGTGCTTTAA

Upstream 100 bases:

>100_bases
GATACTGGCCTGGTTTACCTATATTGGCCTGCTCCAGATTAGAGGTTCCCAACTCTAAGTTGAGGAAAACTTCATATATG
GCTCTCCCCTTCTTCCGAAC

Downstream 100 bases:

>100_bases
AGCATCTCATTCCTCGCATAAGATTCTCTCAAGCCCACATCGAGAATGCAGATGCAGTTCCTTATACGAGCATCCTCGGA
CACACCACTGGTCACCACGG

Product: protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKAVALLSSGIDSPVAIYLMLRKGMEITPVHFKQSDKKESKVLELCEILSKYGKLNEPVIVDAYEEQAPVFSKLVEIGKA
KWTCLFCKYTMLRKACRVGHEIGAKAIVTGDSLGQVASQTLDNLLIISQASDLPILRPLIGRDKEEIVKIAKEIGTFEIS
IEPEEPCPFVPKYPVVRGSLGEFEKIREKLVKEGVL

Sequences:

>Translated_196_residues
MKAVALLSSGIDSPVAIYLMLRKGMEITPVHFKQSDKKESKVLELCEILSKYGKLNEPVIVDAYEEQAPVFSKLVEIGKA
KWTCLFCKYTMLRKACRVGHEIGAKAIVTGDSLGQVASQTLDNLLIISQASDLPILRPLIGRDKEEIVKIAKEIGTFEIS
IEPEEPCPFVPKYPVVRGSLGEFEKIREKLVKEGVL
>Mature_196_residues
MKAVALLSSGIDSPVAIYLMLRKGMEITPVHFKQSDKKESKVLELCEILSKYGKLNEPVIVDAYEEQAPVFSKLVEIGKA
KWTCLFCKYTMLRKACRVGHEIGAKAIVTGDSLGQVASQTLDNLLIISQASDLPILRPLIGRDKEEIVKIAKEIGTFEIS
IEPEEPCPFVPKYPVVRGSLGEFEKIREKLVKEGVL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=189, Percent_Identity=33.8624338624339, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 21760; Mature: 21760

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVALLSSGIDSPVAIYLMLRKGMEITPVHFKQSDKKESKVLELCEILSKYGKLNEPVI
CCHHHHHHCCCCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VDAYEEQAPVFSKLVEIGKAKWTCLFCKYTMLRKACRVGHEIGAKAIVTGDSLGQVASQT
EECHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
LDNLLIISQASDLPILRPLIGRDKEEIVKIAKEIGTFEISIEPEEPCPFVPKYPVVRGSL
HHHEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCH
GEFEKIREKLVKEGVL
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAVALLSSGIDSPVAIYLMLRKGMEITPVHFKQSDKKESKVLELCEILSKYGKLNEPVI
CCHHHHHHCCCCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VDAYEEQAPVFSKLVEIGKAKWTCLFCKYTMLRKACRVGHEIGAKAIVTGDSLGQVASQT
EECHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
LDNLLIISQASDLPILRPLIGRDKEEIVKIAKEIGTFEISIEPEEPCPFVPKYPVVRGSL
HHHEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCH
GEFEKIREKLVKEGVL
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA