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The map label for this gene is cysO [H]

Identifier: 212224153

GI number: 212224153

Start: 927853

End: 928674

Strand: Reverse

Name: cysO [H]

Synonym: TON_1004

Alternate gene names: 212224153

Gene position: 928674-927853 (Counterclockwise)

Preceding gene: 212224155

Following gene: 212224152

Centisome position: 50.26

GC content: 52.8

Gene sequence:

>822_bases
ATGTATTTTGCCAAGTTAGAGTTCTTTAACCCCTTCAGCAGGAGCATTAAGGACAGGGCCGTTTTTAACATGCTTATGAA
GGCCATCGAGCGCGGGGACATCAACGGCACGAGGAAGCTTTTTGAGGCAACTTCCGGCAACGTCGGGATTTCTCTGGCGG
CGCTCAGCAACGTTCTCGGTATTGAGTTCAGGGCATACCTGCCAAAGCCGACACCAAAGGCCACCCAGGTTCTGCTCAGA
GTACTCGGTGCGGATGTTATCATGACTGACTTCGAGACTATAGACCCCACCATGGTTCAGTACGTCGTCGAAGAAGCAAA
GAAGGCTGGAGCGGCTAACCTCAACCAGTTCGAGAACGACGACAACTTCGAGGCCCACTACCGCTTTACCGCCAGGGAGA
TAGACGAGCAGCTGAAGAGCATTGGCAAAAGGCCCGACGTTATGATAGCGGGCATAGGCACTTCGGGCCACATAGCAGGT
CTGGCAAAGTACTTCAAGGAGCGCTATGACACAACCGTTGTGGGCGTCGTCCCGGCGAAGGGTGAGAAGATCCCCGGCAT
AAAGCGCCTTGAAACAAAGCCCAAGTGGTACTTCCAAGTAGACATAGACAGGGTCGTGGAGATAACCCGGAACGAAGCCA
TTGAGGGTGCCCTCAGCGTGGCACGCAGAGACGGTCTCTTGATAGGACTCAGCTCTGGAGCAGTGGTCAAAGCGTACGAA
AAAGTCTCCAGCGAATTCGGTGAGAAAACATACGTCCTGATCTTTCCGGACGATGGATTTAAATACGTGGAGATATTTGA
GGGTTACCTGGGGATCACATGA

Upstream 100 bases:

>100_bases
CTTTTATGGTGATGGCAAATGAACGTGTTTAACAACACACTGGAGCTGTTCGAAAAGTACAAGCCGACCCCCCTTGTTAG
GCTTTCCACCGAGAGGGGGG

Downstream 100 bases:

>100_bases
AAAGGTTAACCCTCGCCACCTACCTTCTTTTCCTCAACGGCTTTCTGCTTCTGTATTATGCCTACTCCTTCGGTTCAGTA
GTTTACCTAGTCTTCGGCCT

Product: cysteine synthase

Products: NA

Alternate protein names: Cystathionine beta-synthase; Cysteine synthase; O-acetylserine sulfhydrylase; O-phosphoserine sulfhydrylase; Serine sulfhydrase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MYFAKLEFFNPFSRSIKDRAVFNMLMKAIERGDINGTRKLFEATSGNVGISLAALSNVLGIEFRAYLPKPTPKATQVLLR
VLGADVIMTDFETIDPTMVQYVVEEAKKAGAANLNQFENDDNFEAHYRFTAREIDEQLKSIGKRPDVMIAGIGTSGHIAG
LAKYFKERYDTTVVGVVPAKGEKIPGIKRLETKPKWYFQVDIDRVVEITRNEAIEGALSVARRDGLLIGLSSGAVVKAYE
KVSSEFGEKTYVLIFPDDGFKYVEIFEGYLGIT

Sequences:

>Translated_273_residues
MYFAKLEFFNPFSRSIKDRAVFNMLMKAIERGDINGTRKLFEATSGNVGISLAALSNVLGIEFRAYLPKPTPKATQVLLR
VLGADVIMTDFETIDPTMVQYVVEEAKKAGAANLNQFENDDNFEAHYRFTAREIDEQLKSIGKRPDVMIAGIGTSGHIAG
LAKYFKERYDTTVVGVVPAKGEKIPGIKRLETKPKWYFQVDIDRVVEITRNEAIEGALSVARRDGLLIGLSSGAVVKAYE
KVSSEFGEKTYVLIFPDDGFKYVEIFEGYLGIT
>Mature_273_residues
MYFAKLEFFNPFSRSIKDRAVFNMLMKAIERGDINGTRKLFEATSGNVGISLAALSNVLGIEFRAYLPKPTPKATQVLLR
VLGADVIMTDFETIDPTMVQYVVEEAKKAGAANLNQFENDDNFEAHYRFTAREIDEQLKSIGKRPDVMIAGIGTSGHIAG
LAKYFKERYDTTVVGVVPAKGEKIPGIKRLETKPKWYFQVDIDRVVEITRNEAIEGALSVARRDGLLIGLSSGAVVKAYE
KVSSEFGEKTYVLIFPDDGFKYVEIFEGYLGIT

Specific function: Cysteine synthase that can also catalyze the synthesis of S-sulfo-L-cysteine from thiosulfate and O(3)-acetyl-L-serine, as well as the sulfhydrylation of L-serine by sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=291, Percent_Identity=28.5223367697594, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI295821200, Length=291, Percent_Identity=28.5223367697594, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI4557415, Length=291, Percent_Identity=28.5223367697594, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI2367138, Length=264, Percent_Identity=32.5757575757576, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788754, Length=287, Percent_Identity=31.0104529616725, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI32566674, Length=274, Percent_Identity=29.9270072992701, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17562970, Length=274, Percent_Identity=29.9270072992701, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535051, Length=270, Percent_Identity=30.3703703703704, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115535073, Length=284, Percent_Identity=31.3380281690141, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17561720, Length=255, Percent_Identity=30.5882352941176, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI32566672, Length=202, Percent_Identity=27.7227722772277, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25147552, Length=263, Percent_Identity=29.277566539924, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=296, Percent_Identity=32.4324324324324, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321449, Length=316, Percent_Identity=31.6455696202532, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24643623, Length=181, Percent_Identity=34.2541436464088, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129101, Length=181, Percent_Identity=34.2541436464088, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.22; =2.5.1.47; =2.5.1.65 [H]

Molecular weight: Translated: 30416; Mature: 30416

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFAKLEFFNPFSRSIKDRAVFNMLMKAIERGDINGTRKLFEATSGNVGISLAALSNVLG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEHHHHHHHHH
IEFRAYLPKPTPKATQVLLRVLGADVIMTDFETIDPTMVQYVVEEAKKAGAANLNQFEND
CEEEEECCCCCCHHHHHHHHHHCCHHEEECCHHCCHHHHHHHHHHHHHCCCCCCHHCCCC
DNFEAHYRFTAREIDEQLKSIGKRPDVMIAGIGTSGHIAGLAKYFKERYDTTVVGVVPAK
CCCEEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECC
GEKIPGIKRLETKPKWYFQVDIDRVVEITRNEAIEGALSVARRDGLLIGLSSGAVVKAYE
CCCCCCCHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHH
KVSSEFGEKTYVLIFPDDGFKYVEIFEGYLGIT
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MYFAKLEFFNPFSRSIKDRAVFNMLMKAIERGDINGTRKLFEATSGNVGISLAALSNVLG
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEHHHHHHHHH
IEFRAYLPKPTPKATQVLLRVLGADVIMTDFETIDPTMVQYVVEEAKKAGAANLNQFEND
CEEEEECCCCCCHHHHHHHHHHCCHHEEECCHHCCHHHHHHHHHHHHHCCCCCCHHCCCC
DNFEAHYRFTAREIDEQLKSIGKRPDVMIAGIGTSGHIAGLAKYFKERYDTTVVGVVPAK
CCCEEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECC
GEKIPGIKRLETKPKWYFQVDIDRVVEITRNEAIEGALSVARRDGLLIGLSSGAVVKAYE
CCCCCCCHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHH
KVSSEFGEKTYVLIFPDDGFKYVEIFEGYLGIT
HHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966; 12644499 [H]