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The map label for this gene is 212223574

Identifier: 212223574

GI number: 212223574

Start: 400644

End: 401303

Strand: Reverse

Name: 212223574

Synonym: TON_0428

Alternate gene names: NA

Gene position: 401303-400644 (Counterclockwise)

Preceding gene: 212223575

Following gene: 212223573

Centisome position: 21.72

GC content: 53.48

Gene sequence:

>660_bases
ATGCTCCACCTGGTGATAGCTGACTCCGAGCTGGAGCTGGCTCCAAAGTCGATAGTTGATCACCCAGCGATAGTCAACTA
CGCCAAGAGGAGGAACAAGAAGCCAGAGGAAGTACTCCTGGACAGCACTTACCACCACTCGGCGCTCAAGAAGCTCGAAG
ACGGTGAGAGGCGCGGGAGGCCAGACATAGTGCACGTCTGCCTCCTCAACGCGCTTGAGAGCATAGCGAACAAGGAGGGC
AAGCTCCGCGTTTACGTCCACACGAGGAACGACGAGGTGATTTACATCAAGCCAGAGACAAGGATTCCGAGGAACTACAA
CCGCTTCGTTGGTCTGATGGAAAGTCTCTTCAAGAACGGTGTTGTGCCTGAGGGACTTGAGCTGCTTCGCATGGAGGAGA
AGAGCCTGGCTGAGCTCATCGACGAAATAAAACCGGACGGAGTCTTCGTCATGCACGAGAACGGGGAGTCCATGAAGCCT
CAGGAGTTTGGAAAGGTCCTTGCAGGACTCCAGAGCCCGCTGGTGGTTGTCGGAGGATTTCCCCACGGAGACTTCAGGAG
TGAAATTCCCGGAAAGAAGATAAGCCTTTACAAAGCCCCATTGATGGCATGGACAGTTGTGAACGAGATAATCATCAACT
TTGAGCATTGGGTTCTCTGA

Upstream 100 bases:

>100_bases
GGTGTTATACCTCCGGAGATACTCGGAATGCGCATAGACACCTTTACACGGATCACTGAGGAACTTGCCGAGAGAGGAAT
AACGCTGGAGAGGTGGGAGA

Downstream 100 bases:

>100_bases
AAAGGGTAGAAGGGCTACATAATGTTTTTATATTTTGCCGATGTTTAATTCTTTTAGGCCTCTTGAAACAACTCCAACTA
TCTCCTCGGGGGTGTAATTC

Product: ribosome biogenesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MLHLVIADSELELAPKSIVDHPAIVNYAKRRNKKPEEVLLDSTYHHSALKKLEDGERRGRPDIVHVCLLNALESIANKEG
KLRVYVHTRNDEVIYIKPETRIPRNYNRFVGLMESLFKNGVVPEGLELLRMEEKSLAELIDEIKPDGVFVMHENGESMKP
QEFGKVLAGLQSPLVVVGGFPHGDFRSEIPGKKISLYKAPLMAWTVVNEIIINFEHWVL

Sequences:

>Translated_219_residues
MLHLVIADSELELAPKSIVDHPAIVNYAKRRNKKPEEVLLDSTYHHSALKKLEDGERRGRPDIVHVCLLNALESIANKEG
KLRVYVHTRNDEVIYIKPETRIPRNYNRFVGLMESLFKNGVVPEGLELLRMEEKSLAELIDEIKPDGVFVMHENGESMKP
QEFGKVLAGLQSPLVVVGGFPHGDFRSEIPGKKISLYKAPLMAWTVVNEIIINFEHWVL
>Mature_219_residues
MLHLVIADSELELAPKSIVDHPAIVNYAKRRNKKPEEVLLDSTYHHSALKKLEDGERRGRPDIVHVCLLNALESIANKEG
KLRVYVHTRNDEVIYIKPETRIPRNYNRFVGLMESLFKNGVVPEGLELLRMEEKSLAELIDEIKPDGVFVMHENGESMKP
QEFGKVLAGLQSPLVVVGGFPHGDFRSEIPGKKISLYKAPLMAWTVVNEIIINFEHWVL

Specific function: Involved in ribosomal biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing

COG id: COG1756

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NEP1 family

Homologues:

Organism=Homo sapiens, GI194328699, Length=182, Percent_Identity=32.967032967033, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17555712, Length=82, Percent_Identity=42.6829268292683, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323215, Length=181, Percent_Identity=31.4917127071823, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24639698, Length=186, Percent_Identity=29.5698924731183, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NEP1_THEON (B6YTM6)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306810.1
- GeneID:   7016723
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0428
- HOGENOM:   HBG512786
- OMA:   RVYVHTR
- ProtClustDB:   PRK04171
- HAMAP:   MF_00554
- InterPro:   IPR005304
- PANTHER:   PTHR12636

Pfam domain/function: PF03587 EMG1

EC number: NA

Molecular weight: Translated: 24908; Mature: 24908

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLVIADSELELAPKSIVDHPAIVNYAKRRNKKPEEVLLDSTYHHSALKKLEDGERRGR
CEEEEEECCCCCCCCHHHHCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCC
PDIVHVCLLNALESIANKEGKLRVYVHTRNDEVIYIKPETRIPRNYNRFVGLMESLFKNG
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHCC
VVPEGLELLRMEEKSLAELIDEIKPDGVFVMHENGESMKPQEFGKVLAGLQSPLVVVGGF
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECC
PHGDFRSEIPGKKISLYKAPLMAWTVVNEIIINFEHWVL
CCCCCHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLHLVIADSELELAPKSIVDHPAIVNYAKRRNKKPEEVLLDSTYHHSALKKLEDGERRGR
CEEEEEECCCCCCCCHHHHCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCC
PDIVHVCLLNALESIANKEGKLRVYVHTRNDEVIYIKPETRIPRNYNRFVGLMESLFKNG
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHCC
VVPEGLELLRMEEKSLAELIDEIKPDGVFVMHENGESMKPQEFGKVLAGLQSPLVVVGGF
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECC
PHGDFRSEIPGKKISLYKAPLMAWTVVNEIIINFEHWVL
CCCCCHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA