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The map label for this gene is hycE [H]

Identifier: 212223422

GI number: 212223422

Start: 241699

End: 243450

Strand: Reverse

Name: hycE [H]

Synonym: TON_0276

Alternate gene names: 212223422

Gene position: 243450-241699 (Counterclockwise)

Preceding gene: 212223423

Following gene: 212223421

Centisome position: 13.18

GC content: 55.71

Gene sequence:

>1752_bases
ATGGAGTGCAGCGTGTGTGCGGGTGGATGCAGATCGGCTGAAGTTGAGGACGTCCTTGAGGATGGTCATCTAAAGGAATT
CGTGGAGAAGTTTAGGGGAGCGATCTTCGAGTGCAAGAAGCTGACGAGGAACCAGTACCTGTTCATCGTTGATAGGGAGG
CACTTCCGGAGATGGTCCTCCACTGGCACAACCATTCCGAGCTAAAAGAAACCCACTTCTCGATGGGAACAGGAACCGAT
GAGAGGAACATCGCCGGAAAGTTCACCTACGCTCCGGTAATAAACGTTGCCGTTGAGCCTGGAAACGGGGAGAGGAACTA
CTGGGTTATTCTGAAGGCCTACCTCGACGAGGACAACCCGGAGTTCCCCTCCATAGCCGCGAAGCTTCCAGCAGCCCTCT
GGGCGGAGAGGGAAGTCTATGATCTGCTTGGCTTCAACCCCAAAGGCCATCCCGACCTGAGGAGGCTCGTCCTGCCGGAG
GACTGGCCGGAGGGTGTTTACCCGCTCAGGAAGGACCATGACTACAAGGCCTCGCCGATGGATACGCCAAAGTGCTACTA
CAAGCCCGGGCCGCCCGACACAATGACGGTTCCGATTGGTCCGTACCACCTGGCGCTCGACGAGCCGGCCCACTTCAGGA
TATTCGTCAAGGGGGAAACGGTGGTTGACGTTGACTACCGCGGCTTCTACTCCCACAGGGGAATCGAGAAGATAGGAGAG
GGAAGACTGACCTACAATCAGGTGCTCTTCATAGCCGAGAGAATATGTGGAATCTGTGGCTTCCAGCACTCGACGAGCTA
CGCCCAGGCGGTTGAAAACATAGCCGGCGTTGAAATCCCCGAGAGGGCCATGTACATAAGGACGATAATGCTGGAGATAG
AAAGGATTCACTCCCACATGCTCTGGGCCGGTGTTGCGGCTCACCTGACGGGCTTTGACACGGGATTCATGCACGCTTGG
CGCGTCAGAGAGCCTGTTATGTGGCTCGCAGAGAGGCTCACAGGAAACAGGAAGACCTACGGAATCAACATCGTCGGAGG
AGTTAGGAGGGACTTCCTCGACTACCGCAAGGAGATGATAATGGAGAAGATCAAGGAGCTCAGGAGGCAGGTCGAAGAGT
TCATCGAAATAGCGACCGGTACGGCAACCTTCGTCAAGAGGGCCGAGGGGGTTGGAATTCTGCCGTACAAGGTGGCCAAG
GCTTACTCAGTCCTTGGTCCGAACGGAAGGGCCAGTGGGAGGAACATTGACATTAGAAGGGATCAGCCGTTCGCAGCATA
CAAGGATTTGGACTTCAAGGTTCCAGTCTACAAGGAGGGCGACGTCTTGGCAAGGTTCCTCATCAGGATGGACGAGGTGC
TCGAGAGCATCTGGATAATAGAGCAGGCCATTGACCAGATGCCGGGAGGAGACGTCTTCGTGCCGATAGGGGAGCTTCCG
GAGTATGAAGAGGCCCTAGGCTACAGTGAAGCTCCAAGGGGCGAAGTCATCCACTACGTCATGACTGACAAGAAGAACAA
GGTCTACCGCTGGAAGGTTAGAGCCCCGACCTACAACAACCTTCCAGCTGTTCCGGAGATGCTCAAGGGCTACAGCGTTG
CCGATGCCCCGCTCATCATAGCGAGCATAGATCCGTGCTACTCCTGTACGGAGAGGGTTCAGATAGTGGACGTTGAGACC
GGAAAGGCCCAGACCCTGAACGAGCAGCAGTTCAACATGCTCTCAATACAGAAGGGCAAGGGGGTGGCCTGA

Upstream 100 bases:

>100_bases
AAACGCCCGCCTGAGGATCGACCAGGCGGTTTCCCTTAACAAGAAGGTCTTCCTGATGTCCCTTGCTGTCCTGATAATAG
CGCTGGTGGGGTGGTGAATT

Downstream 100 bases:

>100_bases
TGGCCCAGGCGATTTCCTTCACCGACAGGCTCAAGTTCTGGAAGCGACCAGAGGAGGACGTTAAGAAGGCTCCCGTCACG
ACTTCTTATCCTTTTGTTGA

Product: Hydrogenase 4, component G or formate hydrogen lyase, subunit 5

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MECSVCAGGCRSAEVEDVLEDGHLKEFVEKFRGAIFECKKLTRNQYLFIVDREALPEMVLHWHNHSELKETHFSMGTGTD
ERNIAGKFTYAPVINVAVEPGNGERNYWVILKAYLDEDNPEFPSIAAKLPAALWAEREVYDLLGFNPKGHPDLRRLVLPE
DWPEGVYPLRKDHDYKASPMDTPKCYYKPGPPDTMTVPIGPYHLALDEPAHFRIFVKGETVVDVDYRGFYSHRGIEKIGE
GRLTYNQVLFIAERICGICGFQHSTSYAQAVENIAGVEIPERAMYIRTIMLEIERIHSHMLWAGVAAHLTGFDTGFMHAW
RVREPVMWLAERLTGNRKTYGINIVGGVRRDFLDYRKEMIMEKIKELRRQVEEFIEIATGTATFVKRAEGVGILPYKVAK
AYSVLGPNGRASGRNIDIRRDQPFAAYKDLDFKVPVYKEGDVLARFLIRMDEVLESIWIIEQAIDQMPGGDVFVPIGELP
EYEEALGYSEAPRGEVIHYVMTDKKNKVYRWKVRAPTYNNLPAVPEMLKGYSVADAPLIIASIDPCYSCTERVQIVDVET
GKAQTLNEQQFNMLSIQKGKGVA

Sequences:

>Translated_583_residues
MECSVCAGGCRSAEVEDVLEDGHLKEFVEKFRGAIFECKKLTRNQYLFIVDREALPEMVLHWHNHSELKETHFSMGTGTD
ERNIAGKFTYAPVINVAVEPGNGERNYWVILKAYLDEDNPEFPSIAAKLPAALWAEREVYDLLGFNPKGHPDLRRLVLPE
DWPEGVYPLRKDHDYKASPMDTPKCYYKPGPPDTMTVPIGPYHLALDEPAHFRIFVKGETVVDVDYRGFYSHRGIEKIGE
GRLTYNQVLFIAERICGICGFQHSTSYAQAVENIAGVEIPERAMYIRTIMLEIERIHSHMLWAGVAAHLTGFDTGFMHAW
RVREPVMWLAERLTGNRKTYGINIVGGVRRDFLDYRKEMIMEKIKELRRQVEEFIEIATGTATFVKRAEGVGILPYKVAK
AYSVLGPNGRASGRNIDIRRDQPFAAYKDLDFKVPVYKEGDVLARFLIRMDEVLESIWIIEQAIDQMPGGDVFVPIGELP
EYEEALGYSEAPRGEVIHYVMTDKKNKVYRWKVRAPTYNNLPAVPEMLKGYSVADAPLIIASIDPCYSCTERVQIVDVET
GKAQTLNEQQFNMLSIQKGKGVA
>Mature_583_residues
MECSVCAGGCRSAEVEDVLEDGHLKEFVEKFRGAIFECKKLTRNQYLFIVDREALPEMVLHWHNHSELKETHFSMGTGTD
ERNIAGKFTYAPVINVAVEPGNGERNYWVILKAYLDEDNPEFPSIAAKLPAALWAEREVYDLLGFNPKGHPDLRRLVLPE
DWPEGVYPLRKDHDYKASPMDTPKCYYKPGPPDTMTVPIGPYHLALDEPAHFRIFVKGETVVDVDYRGFYSHRGIEKIGE
GRLTYNQVLFIAERICGICGFQHSTSYAQAVENIAGVEIPERAMYIRTIMLEIERIHSHMLWAGVAAHLTGFDTGFMHAW
RVREPVMWLAERLTGNRKTYGINIVGGVRRDFLDYRKEMIMEKIKELRRQVEEFIEIATGTATFVKRAEGVGILPYKVAK
AYSVLGPNGRASGRNIDIRRDQPFAAYKDLDFKVPVYKEGDVLARFLIRMDEVLESIWIIEQAIDQMPGGDVFVPIGELP
EYEEALGYSEAPRGEVIHYVMTDKKNKVYRWKVRAPTYNNLPAVPEMLKGYSVADAPLIIASIDPCYSCTERVQIVDVET
GKAQTLNEQQFNMLSIQKGKGVA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=421, Percent_Identity=28.2660332541568, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI260898743, Length=411, Percent_Identity=28.4671532846715, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1789076, Length=573, Percent_Identity=42.9319371727749, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1788832, Length=564, Percent_Identity=41.1347517730496, Blast_Score=467, Evalue=1e-132,
Organism=Escherichia coli, GI145693162, Length=502, Percent_Identity=28.2868525896414, Blast_Score=193, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17568379, Length=387, Percent_Identity=27.1317829457364, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17555284, Length=387, Percent_Identity=26.3565891472868, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24638644, Length=386, Percent_Identity=30.5699481865285, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI221459469, Length=386, Percent_Identity=29.2746113989637, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 66298; Mature: 66298

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECSVCAGGCRSAEVEDVLEDGHLKEFVEKFRGAIFECKKLTRNQYLFIVDREALPEMVL
CCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
HWHNHSELKETHFSMGTGTDERNIAGKFTYAPVINVAVEPGNGERNYWVILKAYLDEDNP
HHCCCHHHHHHHHCCCCCCCCCCCCCEEEECEEEEEEEECCCCCCCEEEEEEEEECCCCC
EFPSIAAKLPAALWAEREVYDLLGFNPKGHPDLRRLVLPEDWPEGVYPLRKDHDYKASPM
CCCHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCCC
DTPKCYYKPGPPDTMTVPIGPYHLALDEPAHFRIFVKGETVVDVDYRGFYSHRGIEKIGE
CCCCCEECCCCCCEEEEECCCEEEEECCCCEEEEEEECCEEEEECCCCHHHHCCHHHHCC
GRLTYNQVLFIAERICGICGFQHSTSYAQAVENIAGVEIPERAMYIRTIMLEIERIHSHM
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LWAGVAAHLTGFDTGFMHAWRVREPVMWLAERLTGNRKTYGINIVGGVRRDFLDYRKEMI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHH
MEKIKELRRQVEEFIEIATGTATFVKRAEGVGILPYKVAKAYSVLGPNGRASGRNIDIRR
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEC
DQPFAAYKDLDFKVPVYKEGDVLARFLIRMDEVLESIWIIEQAIDQMPGGDVFVPIGELP
CCCCHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
EYEEALGYSEAPRGEVIHYVMTDKKNKVYRWKVRAPTYNNLPAVPEMLKGYSVADAPLII
CHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCCCCEEE
ASIDPCYSCTERVQIVDVETGKAQTLNEQQFNMLSIQKGKGVA
EECCHHHHHCCCEEEEEECCCCCCCCCHHHCCEEEEECCCCCC
>Mature Secondary Structure
MECSVCAGGCRSAEVEDVLEDGHLKEFVEKFRGAIFECKKLTRNQYLFIVDREALPEMVL
CCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
HWHNHSELKETHFSMGTGTDERNIAGKFTYAPVINVAVEPGNGERNYWVILKAYLDEDNP
HHCCCHHHHHHHHCCCCCCCCCCCCCEEEECEEEEEEEECCCCCCCEEEEEEEEECCCCC
EFPSIAAKLPAALWAEREVYDLLGFNPKGHPDLRRLVLPEDWPEGVYPLRKDHDYKASPM
CCCHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCCC
DTPKCYYKPGPPDTMTVPIGPYHLALDEPAHFRIFVKGETVVDVDYRGFYSHRGIEKIGE
CCCCCEECCCCCCEEEEECCCEEEEECCCCEEEEEEECCEEEEECCCCHHHHCCHHHHCC
GRLTYNQVLFIAERICGICGFQHSTSYAQAVENIAGVEIPERAMYIRTIMLEIERIHSHM
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LWAGVAAHLTGFDTGFMHAWRVREPVMWLAERLTGNRKTYGINIVGGVRRDFLDYRKEMI
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHH
MEKIKELRRQVEEFIEIATGTATFVKRAEGVGILPYKVAKAYSVLGPNGRASGRNIDIRR
HHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEC
DQPFAAYKDLDFKVPVYKEGDVLARFLIRMDEVLESIWIIEQAIDQMPGGDVFVPIGELP
CCCCHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
EYEEALGYSEAPRGEVIHYVMTDKKNKVYRWKVRAPTYNNLPAVPEMLKGYSVADAPLII
CHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCCCCEEE
ASIDPCYSCTERVQIVDVETGKAQTLNEQQFNMLSIQKGKGVA
EECCHHHHHCCCEEEEEECCCCCCCCCHHHCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]