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The map label for this gene is hycF [H]

Identifier: 212223421

GI number: 212223421

Start: 241082

End: 241699

Strand: Reverse

Name: hycF [H]

Synonym: TON_0275

Alternate gene names: 212223421

Gene position: 241699-241082 (Counterclockwise)

Preceding gene: 212223422

Following gene: 212223420

Centisome position: 13.08

GC content: 56.8

Gene sequence:

>618_bases
ATGGCCCAGGCGATTTCCTTCACCGACAGGCTCAAGTTCTGGAAGCGACCAGAGGAGGACGTTAAGAAGGCTCCCGTCAC
GACTTCTTATCCTTTTGTTGATATCGAAAAGCCGCCGGAATATAGGGGCATACCTCGCATAGATCCTCACCTCTGCATTG
GTTGTGGAGCCTGTGTTAGGGCCTGTCCACCGGACGCGCTCACGATAGAGTGGGACTTCGAGAACGGGAGGAAGAGGATA
GTCTTCAACGCCGCGCGCTGCATAAGGTGCCACCGCTGCGTCGAGGTTTGTCCAACCGGTGCGATGCAGGGCACAACGAG
GTTCGAGATAGCGACGCCGAACAAGGAGGACCTCATCGAGGTCGTTGACCACAAGCTCTACAGATGCCCGCGCTGTGGGC
GGTACGAGGAGTTCACCGAGAGGCAGATAGGGAAGATGTTCCAGATTCTGCCGGAGGAAGTCATTGACCAGCACGGCATA
GCTGAGAGGGCTTTTCTCTGCAGGGAGTGCAGGATGGAGGAGAGCGCCAAGACCTTGGCGGTTCAAGGGCCCTATGCGGA
TAGCCTTCTCCTTTCCCTCTATCCGAGAGGCTCAAAGGTGATGGGTGAGAGGAGATGA

Upstream 100 bases:

>100_bases
AGAGGGTTCAGATAGTGGACGTTGAGACCGGAAAGGCCCAGACCCTGAACGAGCAGCAGTTCAACATGCTCTCAATACAG
AAGGGCAAGGGGGTGGCCTG

Downstream 100 bases:

>100_bases
GCGGGTTGAAGTCCGTTTGGGTCTTCCACGTTGACAGTGGGAGCTGCAACGGCTGCGACATAGAGATACTCGACGTGCTC
ACGCCCTATTACGACGCCGA

Product: Formate hydrogen lyase subunit 6

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 6; Hydrogenase-3 component F [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MAQAISFTDRLKFWKRPEEDVKKAPVTTSYPFVDIEKPPEYRGIPRIDPHLCIGCGACVRACPPDALTIEWDFENGRKRI
VFNAARCIRCHRCVEVCPTGAMQGTTRFEIATPNKEDLIEVVDHKLYRCPRCGRYEEFTERQIGKMFQILPEEVIDQHGI
AERAFLCRECRMEESAKTLAVQGPYADSLLLSLYPRGSKVMGERR

Sequences:

>Translated_205_residues
MAQAISFTDRLKFWKRPEEDVKKAPVTTSYPFVDIEKPPEYRGIPRIDPHLCIGCGACVRACPPDALTIEWDFENGRKRI
VFNAARCIRCHRCVEVCPTGAMQGTTRFEIATPNKEDLIEVVDHKLYRCPRCGRYEEFTERQIGKMFQILPEEVIDQHGI
AERAFLCRECRMEESAKTLAVQGPYADSLLLSLYPRGSKVMGERR
>Mature_204_residues
AQAISFTDRLKFWKRPEEDVKKAPVTTSYPFVDIEKPPEYRGIPRIDPHLCIGCGACVRACPPDALTIEWDFENGRKRIV
FNAARCIRCHRCVEVCPTGAMQGTTRFEIATPNKEDLIEVVDHKLYRCPRCGRYEEFTERQIGKMFQILPEEVIDQHGIA
ERAFLCRECRMEESAKTLAVQGPYADSLLLSLYPRGSKVMGERR

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789075, Length=114, Percent_Identity=42.1052631578947, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788833, Length=114, Percent_Identity=43.859649122807, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 23543; Mature: 23412

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
5.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAISFTDRLKFWKRPEEDVKKAPVTTSYPFVDIEKPPEYRGIPRIDPHLCIGCGACVR
CCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCHHEEECCHHHH
ACPPDALTIEWDFENGRKRIVFNAARCIRCHRCVEVCPTGAMQGTTRFEIATPNKEDLIE
CCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHH
VVDHKLYRCPRCGRYEEFTERQIGKMFQILPEEVIDQHGIAERAFLCRECRMEESAKTLA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEE
VQGPYADSLLLSLYPRGSKVMGERR
EECCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AQAISFTDRLKFWKRPEEDVKKAPVTTSYPFVDIEKPPEYRGIPRIDPHLCIGCGACVR
CCCHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCHHEEECCHHHH
ACPPDALTIEWDFENGRKRIVFNAARCIRCHRCVEVCPTGAMQGTTRFEIATPNKEDLIE
CCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHH
VVDHKLYRCPRCGRYEEFTERQIGKMFQILPEEVIDQHGIAERAFLCRECRMEESAKTLA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEE
VQGPYADSLLLSLYPRGSKVMGERR
EECCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]