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The map label for this gene is hyfC [H]

Identifier: 212223423

GI number: 212223423

Start: 243454

End: 244356

Strand: Reverse

Name: hyfC [H]

Synonym: TON_0277

Alternate gene names: 212223423

Gene position: 244356-243454 (Counterclockwise)

Preceding gene: 212223424

Following gene: 212223422

Centisome position: 13.23

GC content: 54.93

Gene sequence:

>903_bases
ATGGACTACGTAAGCATTATCGCTGCTCCGATCGTCCTCTTCCTCCTTCCACCGTTCCTTGACGGAATAGGGAGAAGGAT
AAAGGCGAGGATTCAGTACAGGAGAGGACCGCCTATAATGCAGACGTTCTACGACCTCGAAAAGCTTCTCAAGCTGCCGT
CAGTGCTTCCAACTGAGGGCCCAATCTTCAGGCTGGCCCCGTACATAGCCCTGGCATCTGCCATTGCCGGCGGCCTAATG
CTTCCCTTCGGAAGCGAGCCGGTGTTGGCTTTTGGAAAGAGCCTCATAGTGTTCTTCTACGTCATGGCGATGGTCAGCGT
AGTGATGATACTTGCTGCTTTCTCCGTCCAGAACGCGTTCTCTCACATAGGTGGACACAGAGAGGTCATGCTGATACTCT
CGATTGAGCCAGTGCTGGCCGTCGTCTTCGGTGTCCTGGCATTCAAGCTTGGAACGCTCAACGTCGCTGAGATGCCTTTC
AGTGCTAACCTCTCGCTTTCCGTTGCCCTAGCTTACATCTTGCTGGCTTACGCGGTCTACGTTGAGGGCGGATTCGTTCC
ATTTGACATAGCTGAGGCAGAAACCGAAGTAATCGGGGGCCCGCTCACCGAGTACAGCGGAAGGCTCCTCGGAGTCTTCA
AGTACGCCCTGCTCGTCAAGAGGGTTGTCCTGCTCTGGCTGCTGGCGTCTATGATTGTGATTCCCGCCATGAGGTCTCTC
GGTATAACAAGCTCAATGGCACTGCTCGTCGCCCAGCTGGTCGTTACGTTTCTGCTTTATTCGCTTGCCGTGGCCGTTGA
GGCTGCAAACGCCCGCCTGAGGATCGACCAGGCGGTTTCCCTTAACAAGAAGGTCTTCCTGATGTCCCTTGCTGTCCTGA
TAATAGCGCTGGTGGGGTGGTGA

Upstream 100 bases:

>100_bases
CTGGCAGCGGGCATCTCGGTGTCCATGGACGAGCTCTTCCTGGCTTCAATGCTGACCACGGTGATAATACTCGCACTCCT
TGTGTTGTGAGGTGATGGTG

Downstream 100 bases:

>100_bases
ATTATGGAGTGCAGCGTGTGTGCGGGTGGATGCAGATCGGCTGAAGTTGAGGACGTCCTTGAGGATGGTCATCTAAAGGA
ATTCGTGGAGAAGTTTAGGG

Product: Hydrogenase 4, component C or formate hydrogen lyase, subunit 4

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDYVSIIAAPIVLFLLPPFLDGIGRRIKARIQYRRGPPIMQTFYDLEKLLKLPSVLPTEGPIFRLAPYIALASAIAGGLM
LPFGSEPVLAFGKSLIVFFYVMAMVSVVMILAAFSVQNAFSHIGGHREVMLILSIEPVLAVVFGVLAFKLGTLNVAEMPF
SANLSLSVALAYILLAYAVYVEGGFVPFDIAEAETEVIGGPLTEYSGRLLGVFKYALLVKRVVLLWLLASMIVIPAMRSL
GITSSMALLVAQLVVTFLLYSLAVAVEAANARLRIDQAVSLNKKVFLMSLAVLIIALVGW

Sequences:

>Translated_300_residues
MDYVSIIAAPIVLFLLPPFLDGIGRRIKARIQYRRGPPIMQTFYDLEKLLKLPSVLPTEGPIFRLAPYIALASAIAGGLM
LPFGSEPVLAFGKSLIVFFYVMAMVSVVMILAAFSVQNAFSHIGGHREVMLILSIEPVLAVVFGVLAFKLGTLNVAEMPF
SANLSLSVALAYILLAYAVYVEGGFVPFDIAEAETEVIGGPLTEYSGRLLGVFKYALLVKRVVLLWLLASMIVIPAMRSL
GITSSMALLVAQLVVTFLLYSLAVAVEAANARLRIDQAVSLNKKVFLMSLAVLIIALVGW
>Mature_300_residues
MDYVSIIAAPIVLFLLPPFLDGIGRRIKARIQYRRGPPIMQTFYDLEKLLKLPSVLPTEGPIFRLAPYIALASAIAGGLM
LPFGSEPVLAFGKSLIVFFYVMAMVSVVMILAAFSVQNAFSHIGGHREVMLILSIEPVLAVVFGVLAFKLGTLNVAEMPF
SANLSLSVALAYILLAYAVYVEGGFVPFDIAEAETEVIGGPLTEYSGRLLGVFKYALLVKRVVLLWLLASMIVIPAMRSL
GITSSMALLVAQLVVTFLLYSLAVAVEAANARLRIDQAVSLNKKVFLMSLAVLIIALVGW

Specific function: Unknown

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI87082115, Length=224, Percent_Identity=35.7142857142857, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789077, Length=285, Percent_Identity=32.9824561403509, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 32481; Mature: 32481

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYVSIIAAPIVLFLLPPFLDGIGRRIKARIQYRRGPPIMQTFYDLEKLLKLPSVLPTEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
PIFRLAPYIALASAIAGGLMLPFGSEPVLAFGKSLIVFFYVMAMVSVVMILAAFSVQNAF
CHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHIGGHREVMLILSIEPVLAVVFGVLAFKLGTLNVAEMPFSANLSLSVALAYILLAYAVY
HHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VEGGFVPFDIAEAETEVIGGPLTEYSGRLLGVFKYALLVKRVVLLWLLASMIVIPAMRSL
HCCCCCEEEHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GITSSMALLVAQLVVTFLLYSLAVAVEAANARLRIDQAVSLNKKVFLMSLAVLIIALVGW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDYVSIIAAPIVLFLLPPFLDGIGRRIKARIQYRRGPPIMQTFYDLEKLLKLPSVLPTEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
PIFRLAPYIALASAIAGGLMLPFGSEPVLAFGKSLIVFFYVMAMVSVVMILAAFSVQNAF
CHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHIGGHREVMLILSIEPVLAVVFGVLAFKLGTLNVAEMPFSANLSLSVALAYILLAYAVY
HHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VEGGFVPFDIAEAETEVIGGPLTEYSGRLLGVFKYALLVKRVVLLWLLASMIVIPAMRSL
HCCCCCEEEHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GITSSMALLVAQLVVTFLLYSLAVAVEAANARLRIDQAVSLNKKVFLMSLAVLIIALVGW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]