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The map label for this gene is livM [H]

Identifier: 212223304

GI number: 212223304

Start: 135158

End: 136186

Strand: Reverse

Name: livM [H]

Synonym: TON_0158

Alternate gene names: 212223304

Gene position: 136186-135158 (Counterclockwise)

Preceding gene: 212223305

Following gene: 212223302

Centisome position: 7.37

GC content: 48.1

Gene sequence:

>1029_bases
ATGATCATGGAACTGCTCCAGCTCATACTCGTCTGGATTGGGATATATGCGGTAGTAAACATAAGCCTGAACATTGAGTA
CGGTTACACAGGAATACCAAACTTCGGAAGGCACTTCGCCGTCCTTATAGGGGCGATTATGCTTGGTGGAGCTGTAAATC
GTATCCTCATGGCTATGCTCAGAATCGACGGCGATCTTATAACTGGAACCACCGATCTCGTCTTCAAAGCCAACAGCCTC
TTCGCAGTCCACCCCATCTATGGACTGGTTTATTTCTTTGGCTCTCTAACATTGGCTTTCCTGTTCGGGGCCCTCTTGGG
TGCCCTCTTCATACTACCGAGCGCTAGGCTCAAGGGGGATTACCTCGGAATGACGCTCTTGGCCATAGGTGAGGTAACGT
TTCTGCTGGCCTACTACACTCCATCTTTAATGGGCGGGTACTATGGAACAGCGGTTCCAGACCCACTAGCCTTTTTGTCG
GGAAGCAACAGGGAGTGGGTATTCATAGGGCTTGTTCTCCTGGTAATGGTACTGGTATTCATATTCGCTGAGAAACTTCT
CAACACACCCTTTGGAAGACTGTTGAAGGCCCACAGGGAGAACGAGGAAGTTGTGAAGGCTTTCGGTCGGGACGTTATGA
GGATTAGGATTAAAGCCTCAATTATTGGCTCGGGAATAGCAGCTCTCGGCGGAGTCCTCTATGGCTTCCATGCTCTTAAC
CTGATAAGTAGCGCATTCACTAGGATTGAGTGGGCGTTCTACCCATTCATCATGATACTCCTAGGGGGAAGGGGCAATAA
CAGAGGAGTTCTCCTTGGAACAGCTATCTTTGTGACGCTGAAAGTTCTAATAGAGACTTACAAGTTCCAGCTGGTACAGC
TACTACACATACCCTTTGAGCCAGTATGGCTAGAATACATTCTATTTGGTGTTTTAGCCCTATTGATAATCTACTACAGG
CCGGAGGGGTTAATAAGAGAGGGTCAGATACTGACAGGGCCAATAAAGCGGTTAAGAGACGGAAGGTAA

Upstream 100 bases:

>100_bases
TCGTCTACGGAAAGGTGGTTTCTCTCATGATAATGATAGCCGTACTACTTCTGATGCCCGAGGGGATTACCGGCACAAAG
GTCTGGAGGAGGTTGATGAG

Downstream 100 bases:

>100_bases
ACCCATAGTCCCCATTTCTGTCCCTAGCTGCCTCGGGGACAATCTCCTCTTTTTATCATTAGCGAAAAATGCCAGCAGGT
TTACGTGATGGAAAGAAGGC

Product: branched-chain amino acid transport ATP-binding protein livM

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MIMELLQLILVWIGIYAVVNISLNIEYGYTGIPNFGRHFAVLIGAIMLGGAVNRILMAMLRIDGDLITGTTDLVFKANSL
FAVHPIYGLVYFFGSLTLAFLFGALLGALFILPSARLKGDYLGMTLLAIGEVTFLLAYYTPSLMGGYYGTAVPDPLAFLS
GSNREWVFIGLVLLVMVLVFIFAEKLLNTPFGRLLKAHRENEEVVKAFGRDVMRIRIKASIIGSGIAALGGVLYGFHALN
LISSAFTRIEWAFYPFIMILLGGRGNNRGVLLGTAIFVTLKVLIETYKFQLVQLLHIPFEPVWLEYILFGVLALLIIYYR
PEGLIREGQILTGPIKRLRDGR

Sequences:

>Translated_342_residues
MIMELLQLILVWIGIYAVVNISLNIEYGYTGIPNFGRHFAVLIGAIMLGGAVNRILMAMLRIDGDLITGTTDLVFKANSL
FAVHPIYGLVYFFGSLTLAFLFGALLGALFILPSARLKGDYLGMTLLAIGEVTFLLAYYTPSLMGGYYGTAVPDPLAFLS
GSNREWVFIGLVLLVMVLVFIFAEKLLNTPFGRLLKAHRENEEVVKAFGRDVMRIRIKASIIGSGIAALGGVLYGFHALN
LISSAFTRIEWAFYPFIMILLGGRGNNRGVLLGTAIFVTLKVLIETYKFQLVQLLHIPFEPVWLEYILFGVLALLIIYYR
PEGLIREGQILTGPIKRLRDGR
>Mature_342_residues
MIMELLQLILVWIGIYAVVNISLNIEYGYTGIPNFGRHFAVLIGAIMLGGAVNRILMAMLRIDGDLITGTTDLVFKANSL
FAVHPIYGLVYFFGSLTLAFLFGALLGALFILPSARLKGDYLGMTLLAIGEVTFLLAYYTPSLMGGYYGTAVPDPLAFLS
GSNREWVFIGLVLLVMVLVFIFAEKLLNTPFGRLLKAHRENEEVVKAFGRDVMRIRIKASIIGSGIAALGGVLYGFHALN
LISSAFTRIEWAFYPFIMILLGGRGNNRGVLLGTAIFVTLKVLIETYKFQLVQLLHIPFEPVWLEYILFGVLALLIIYYR
PEGLIREGQILTGPIKRLRDGR

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=281, Percent_Identity=28.8256227758007, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 37908; Mature: 37908

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMELLQLILVWIGIYAVVNISLNIEYGYTGIPNFGRHFAVLIGAIMLGGAVNRILMAML
CHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RIDGDLITGTTDLVFKANSLFAVHPIYGLVYFFGSLTLAFLFGALLGALFILPSARLKGD
HHCCCEEECCHHHEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YLGMTLLAIGEVTFLLAYYTPSLMGGYYGTAVPDPLAFLSGSNREWVFIGLVLLVMVLVF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHH
IFAEKLLNTPFGRLLKAHRENEEVVKAFGRDVMRIRIKASIIGSGIAALGGVLYGFHALN
HHHHHHHCCCHHHHHHHHCCCHHHHHHHCCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHH
LISSAFTRIEWAFYPFIMILLGGRGNNRGVLLGTAIFVTLKVLIETYKFQLVQLLHIPFE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVWLEYILFGVLALLIIYYRPEGLIREGQILTGPIKRLRDGR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHCCCC
>Mature Secondary Structure
MIMELLQLILVWIGIYAVVNISLNIEYGYTGIPNFGRHFAVLIGAIMLGGAVNRILMAML
CHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RIDGDLITGTTDLVFKANSLFAVHPIYGLVYFFGSLTLAFLFGALLGALFILPSARLKGD
HHCCCEEECCHHHEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YLGMTLLAIGEVTFLLAYYTPSLMGGYYGTAVPDPLAFLSGSNREWVFIGLVLLVMVLVF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHH
IFAEKLLNTPFGRLLKAHRENEEVVKAFGRDVMRIRIKASIIGSGIAALGGVLYGFHALN
HHHHHHHCCCHHHHHHHHCCCHHHHHHHCCHHHHHEEHHHHHHHHHHHHHHHHHHHHHHH
LISSAFTRIEWAFYPFIMILLGGRGNNRGVLLGTAIFVTLKVLIETYKFQLVQLLHIPFE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVWLEYILFGVLALLIIYYRPEGLIREGQILTGPIKRLRDGR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]