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The map label for this gene is mtnN [C]

Identifier: 209551876

GI number: 209551876

Start: 4475305

End: 4475943

Strand: Reverse

Name: mtnN [C]

Synonym: Rleg2_4309

Alternate gene names: 209551876

Gene position: 4475943-4475305 (Counterclockwise)

Preceding gene: 209551877

Following gene: 209551875

Centisome position: 98.63

GC content: 63.22

Gene sequence:

>639_bases
ATGAAGTTCGAGTTGAAGTCGGTCGCGGGTAAATCCATGCTGTTCGTCATGGCGGCAGAGGCCGAATACGGCCCCTTCCT
GCGCTCGCGCATCGAACCCCTGATGACCGGCGTCGGCCCGGTCGAGGCGGCAGTGGCGCTGACCAAGACGCTGGCGCGGC
TGGATGCCGCCGACGACCTGCCGGATCTCGTGGTTTCCCTCGGCTCGGCCGGCTCGGCGAAACTCGAGCAGACCGAAATC
TATCAGGTAACCTCGGTTTCCTACCGCGACATGGACGCCTCGCCGCTCGGCTTCGAAAAGGGCAGGACACCCTTTCTCGA
CCTGCCGGCGGTACTCGAGCTGCCGCTGCGCATTCCAGGTATTCCCGAAGCAAGCCTTTCCACCGGCGGCAACGTGATTT
CGGGTGCGGCCTACAGCGCCATCGACGCCGACATGGTCGACATGGAGACCTTTGCGGTGCTGCGCGCCTGTCAGGGCTAC
AAGCTGCCGCTGATTGGCCTTCGCGGCATTTCCGACGGCGCCGTCGAACTGCAGCATATTTCCGGCTGGACCGAATATCT
GCACATCGTCGACCGCAAGCTTTCCTACGGCGTCGACAGCCTGTTCACGGCGCTGGAGGACGGGGTTTTCTGGTTCTAG

Upstream 100 bases:

>100_bases
CCGACGGACGAGGCTACGCGATCAGCGACGGGCCGGCGAAACTCATCGCCTCCATGACCGGGACGATGATGGTGATCCGC
GGTAGAGAAGGAATTACGGG

Downstream 100 bases:

>100_bases
GCCCTAAAGCCGCGCGTCTTTCAGACGCGCGAGGGACAATCGGAACAATAAAAACCCGACGCCGCCGATTTCCCGGATTG
CAAGGCGGAGCGCTTTTCAT

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: 5-methylthio-D-ribose; adenine

Alternate protein names: Nucleoside Phosphorylase; MTA/SAH Nucleosidase; Nucleoside Phosphorylase-Like Protein; Nucleosidase; Phosphorylase; 5-Methylthioadenosine/S-Adenosylhomocysteinenucl Eosidase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKFELKSVAGKSMLFVMAAEAEYGPFLRSRIEPLMTGVGPVEAAVALTKTLARLDAADDLPDLVVSLGSAGSAKLEQTEI
YQVTSVSYRDMDASPLGFEKGRTPFLDLPAVLELPLRIPGIPEASLSTGGNVISGAAYSAIDADMVDMETFAVLRACQGY
KLPLIGLRGISDGAVELQHISGWTEYLHIVDRKLSYGVDSLFTALEDGVFWF

Sequences:

>Translated_212_residues
MKFELKSVAGKSMLFVMAAEAEYGPFLRSRIEPLMTGVGPVEAAVALTKTLARLDAADDLPDLVVSLGSAGSAKLEQTEI
YQVTSVSYRDMDASPLGFEKGRTPFLDLPAVLELPLRIPGIPEASLSTGGNVISGAAYSAIDADMVDMETFAVLRACQGY
KLPLIGLRGISDGAVELQHISGWTEYLHIVDRKLSYGVDSLFTALEDGVFWF
>Mature_212_residues
MKFELKSVAGKSMLFVMAAEAEYGPFLRSRIEPLMTGVGPVEAAVALTKTLARLDAADDLPDLVVSLGSAGSAKLEQTEI
YQVTSVSYRDMDASPLGFEKGRTPFLDLPAVLELPLRIPGIPEASLSTGGNVISGAAYSAIDADMVDMETFAVLRACQGY
KLPLIGLRGISDGAVELQHISGWTEYLHIVDRKLSYGVDSLFTALEDGVFWF

Specific function: Responsible For Cleavage Of The Glycosidic Bond In Both 5'-Methylthioadenosine (Mta) And S-Adenosylhomocysteine (Sah). [C]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.2.16; 3.2.2.9

Molecular weight: Translated: 22780; Mature: 22780

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFELKSVAGKSMLFVMAAEAEYGPFLRSRIEPLMTGVGPVEAAVALTKTLARLDAADDL
CCCCCCHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCH
PDLVVSLGSAGSAKLEQTEIYQVTSVSYRDMDASPLGFEKGRTPFLDLPAVLELPLRIPG
HHHHHHHCCCCCCCCCHHHEEEEEECEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
IPEASLSTGGNVISGAAYSAIDADMVDMETFAVLRACQGYKLPLIGLRGISDGAVELQHI
CCCCCCCCCCCEECCCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEHHH
SGWTEYLHIVDRKLSYGVDSLFTALEDGVFWF
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKFELKSVAGKSMLFVMAAEAEYGPFLRSRIEPLMTGVGPVEAAVALTKTLARLDAADDL
CCCCCCHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCH
PDLVVSLGSAGSAKLEQTEIYQVTSVSYRDMDASPLGFEKGRTPFLDLPAVLELPLRIPG
HHHHHHHCCCCCCCCCHHHEEEEEECEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
IPEASLSTGGNVISGAAYSAIDADMVDMETFAVLRACQGYKLPLIGLRGISDGAVELQHI
CCCCCCCCCCCEECCCHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEHHH
SGWTEYLHIVDRKLSYGVDSLFTALEDGVFWF
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1.8 {5'-methylthioadenosine}} 0.0004 {5'-methylthioadenosine}} 0.00031 {5'-methylthioadenosine}} 3 {S-adenosylhomocysteine}} 0.0043 {S-adenosylhomocysteine}} [C]

Substrates: methylthioadenosine; H2O

Specific reaction: methylthioadenosine + H2O = 5-methylthio-D-ribose + adenine

General reaction: N-Glycosyl bond hydrolysis [C]

Inhibitor: 5'-Chlor of ormycin; 5'-Ethyl thioadenosine; 5'-Methyl thi of ormycin; 5'-Methyl thioinosine; 5'-Methyl thiotubercidin; 5'-n-Propyl thioadenosine; Adenine; AMP; L-Methionine; N-2-Hydroxy ethyl piperazine-N'-2-ethanesulfonic acid; S-8-Aza-Adenosyl homocyst

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA