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The map label for this gene is phnI [H]

Identifier: 209551693

GI number: 209551693

Start: 4302572

End: 4303678

Strand: Reverse

Name: phnI [H]

Synonym: Rleg2_4122

Alternate gene names: 209551693

Gene position: 4303678-4302572 (Counterclockwise)

Preceding gene: 209551694

Following gene: 209551692

Centisome position: 94.84

GC content: 65.58

Gene sequence:

>1107_bases
ATGTATGTTGCCGTCAAGGGTGGCGAGGCCGCCATCGCCAATGCCCACCGCCTGCTCGCCGACCGCCGCCGCGGCGACCG
TTCGCTGCCGGCAATCGGCATTGACCAGATCGTGGCGCAGCTGGCGCTCGCCGTCGACCGGGTCATGGCCGAGGCCTCGC
TTTTCGACCGCACGCTCGCAGCACTCGCCGTCCGCCAGTCGCGCGGCGATATGATCGAGGCGATCTTCCTGCTGCGCGCC
TATCGCACGACGCTGCCGCGTTTCGGCTATTCGAGGCCGCTCGACACGGCTGATATGACGATCGAACGCCGGATCTCGGC
GACCTACAAGGATCTGCCGGGCGGCCAGCTTCTGGGACCCACCTTCGATTATACCCACCGCCTGCTCGATCCCTCGCTGC
TTGAGGACGAGGCGGTCGAAGCGCCCGCCCAGCGCGCCGCCGAAACCGGCCGCGTCATGCGGGTCTCCGAAATCCTCGGC
GAGGAAGGCCTGATCGAGGCCGACGGCGACATGCCCGAAGATCACGAGATCGGTGATCTCACCCGCGAGCCGATGGAATT
CCCGATGACCCGCGATCTGCGCCTGCAGGCGCTCGCCCGCGGCGACGAAGGTTTCCTGCTGGCGCTCGGCTATTCCACCC
AGCGCGGTTATGGCCGCAACCACCCCTTTACAGGGGAGATCCGCATCGGCGAGGTCGAGGTGGAATTCGATGTGCCGGAA
CTCGGCTTCGCCGTTTCGCTCGGCGTGATCCAGGTCACCGAGTGCCAGATGGTCAATCAGTTCAAGGGTTCGGCCAAGGC
GCCGCCGCAATTCACCCGCGGCTACGGCCTGGTCTTCGGCCAGAGCGAGCGCAAGGCGATGGCGATGTCGCTGGTGGATC
GCGCATTGCGGGCTGAAGAGCTCGGCGAGGATATCACCGCGCCGGCCCAGGACGAGGAATTCGTCATCTCCCATTCCGAC
AACGTCCAGGCGACCGGCTTCGTCGAGCACCTGAAGCTTCCGCATTATGTGGACTTCCAAGCCGAACTCGATCTCGTCCG
CCGCATGCGCCGTGAATTCGAAGCCGCCCGCAGCAGCGGCGAAGATATGAAGGAAGCCGCCGAATGA

Upstream 100 bases:

>100_bases
GCGCTCTTCCCGCGCGGCATCGACATCGTGCTGACATCGGCGACACGTTTTCTCTGCCTGCCGCGCACCACCAAGATCAC
AGCAACGGAGATCTGAGCTC

Downstream 100 bases:

>100_bases
GCGATCTGGCCAGCTACAACTTCGCCTATCTCGACGAACAGACCAAGCGGATGATCCGCCGCGCCATCCTGAAGGCGATC
GCCATACCCGGCTACCAGGT

Product: phosphonate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MYVAVKGGEAAIANAHRLLADRRRGDRSLPAIGIDQIVAQLALAVDRVMAEASLFDRTLAALAVRQSRGDMIEAIFLLRA
YRTTLPRFGYSRPLDTADMTIERRISATYKDLPGGQLLGPTFDYTHRLLDPSLLEDEAVEAPAQRAAETGRVMRVSEILG
EEGLIEADGDMPEDHEIGDLTREPMEFPMTRDLRLQALARGDEGFLLALGYSTQRGYGRNHPFTGEIRIGEVEVEFDVPE
LGFAVSLGVIQVTECQMVNQFKGSAKAPPQFTRGYGLVFGQSERKAMAMSLVDRALRAEELGEDITAPAQDEEFVISHSD
NVQATGFVEHLKLPHYVDFQAELDLVRRMRREFEAARSSGEDMKEAAE

Sequences:

>Translated_368_residues
MYVAVKGGEAAIANAHRLLADRRRGDRSLPAIGIDQIVAQLALAVDRVMAEASLFDRTLAALAVRQSRGDMIEAIFLLRA
YRTTLPRFGYSRPLDTADMTIERRISATYKDLPGGQLLGPTFDYTHRLLDPSLLEDEAVEAPAQRAAETGRVMRVSEILG
EEGLIEADGDMPEDHEIGDLTREPMEFPMTRDLRLQALARGDEGFLLALGYSTQRGYGRNHPFTGEIRIGEVEVEFDVPE
LGFAVSLGVIQVTECQMVNQFKGSAKAPPQFTRGYGLVFGQSERKAMAMSLVDRALRAEELGEDITAPAQDEEFVISHSD
NVQATGFVEHLKLPHYVDFQAELDLVRRMRREFEAARSSGEDMKEAAE
>Mature_368_residues
MYVAVKGGEAAIANAHRLLADRRRGDRSLPAIGIDQIVAQLALAVDRVMAEASLFDRTLAALAVRQSRGDMIEAIFLLRA
YRTTLPRFGYSRPLDTADMTIERRISATYKDLPGGQLLGPTFDYTHRLLDPSLLEDEAVEAPAQRAAETGRVMRVSEILG
EEGLIEADGDMPEDHEIGDLTREPMEFPMTRDLRLQALARGDEGFLLALGYSTQRGYGRNHPFTGEIRIGEVEVEFDVPE
LGFAVSLGVIQVTECQMVNQFKGSAKAPPQFTRGYGLVFGQSERKAMAMSLVDRALRAEELGEDITAPAQDEEFVISHSD
NVQATGFVEHLKLPHYVDFQAELDLVRRMRREFEAARSSGEDMKEAAE

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3626

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnI family [H]

Homologues:

Organism=Escherichia coli, GI1790537, Length=357, Percent_Identity=60.7843137254902, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008773 [H]

Pfam domain/function: PF05861 PhnI [H]

EC number: NA

Molecular weight: Translated: 40818; Mature: 40818

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVAVKGGEAAIANAHRLLADRRRGDRSLPAIGIDQIVAQLALAVDRVMAEASLFDRTLA
CEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAVRQSRGDMIEAIFLLRAYRTTLPRFGYSRPLDTADMTIERRISATYKDLPGGQLLGP
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TFDYTHRLLDPSLLEDEAVEAPAQRAAETGRVMRVSEILGEEGLIEADGDMPEDHEIGDL
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHCCCCEEECCCCCCCCCCCCCC
TREPMEFPMTRDLRLQALARGDEGFLLALGYSTQRGYGRNHPFTGEIRIGEVEVEFDVPE
CCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCC
LGFAVSLGVIQVTECQMVNQFKGSAKAPPQFTRGYGLVFGQSERKAMAMSLVDRALRAEE
CCCEEEECEEEEHHHHHHHHHCCCCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHH
LGEDITAPAQDEEFVISHSDNVQATGFVEHLKLPHYVDFQAELDLVRRMRREFEAARSSG
HCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHCCC
EDMKEAAE
HHHHHHCC
>Mature Secondary Structure
MYVAVKGGEAAIANAHRLLADRRRGDRSLPAIGIDQIVAQLALAVDRVMAEASLFDRTLA
CEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAVRQSRGDMIEAIFLLRAYRTTLPRFGYSRPLDTADMTIERRISATYKDLPGGQLLGP
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TFDYTHRLLDPSLLEDEAVEAPAQRAAETGRVMRVSEILGEEGLIEADGDMPEDHEIGDL
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHCCCCEEECCCCCCCCCCCCCC
TREPMEFPMTRDLRLQALARGDEGFLLALGYSTQRGYGRNHPFTGEIRIGEVEVEFDVPE
CCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCC
LGFAVSLGVIQVTECQMVNQFKGSAKAPPQFTRGYGLVFGQSERKAMAMSLVDRALRAEE
CCCEEEECEEEEHHHHHHHHHCCCCCCCCCHHCCCEEEECCCHHHHHHHHHHHHHHHHHH
LGEDITAPAQDEEFVISHSDNVQATGFVEHLKLPHYVDFQAELDLVRRMRREFEAARSSG
HCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHCCC
EDMKEAAE
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]