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The map label for this gene is phnJ [H]

Identifier: 209551692

GI number: 209551692

Start: 4301700

End: 4302575

Strand: Reverse

Name: phnJ [H]

Synonym: Rleg2_4121

Alternate gene names: 209551692

Gene position: 4302575-4301700 (Counterclockwise)

Preceding gene: 209551693

Following gene: 209551691

Centisome position: 94.81

GC content: 61.53

Gene sequence:

>876_bases
ATGAGCGATCTGGCCAGCTACAACTTCGCCTATCTCGACGAACAGACCAAGCGGATGATCCGCCGCGCCATCCTGAAGGC
GATCGCCATACCCGGCTACCAGGTTCCCTTCGCCTCGCGCGAAATGCCGATGCCCTACGGCTGGGGCACCGGCGGCGTGC
AGGTGACGGCCTCGATCATCGGGCCGGATGACGTGCTGAAAGTCATCGACCAGGGTGCCGACGACACGACCAATGCCGTT
TCCATCCGGGCTTTCTTCCAGAAGGTCGCCAATGTCGCGGTGACGACGCGAACCAGTGATGCGACGATCATCCAGACGCG
CCACCGCATTCCGGAAGAAAAGCTTAAGGACGGCCAGGTGCTCGTCTATCAGGTGCCGATCCCCGAGCCGCTGCGCTTCC
TCGAGCCGCGCGAGACCGAGACCCGCAAGATGCACGCGCTCGAAGAATACGGCCTGATGCATGTGAAACTCTATGAGGAT
ATCGCCCATAACGGCCGGATCTCCAGGACCTATGCCTATCCTGTCAAGGTGCACGGCCGCTACGTGATGGACCCTTCGCC
GACCCCGAAATTCGACAATCCGAAGATGCACATGTCGGATGCGCTGCAGCTCTTCGGCGCCGGACGCGAGAAGCGCATCT
ATGCGGTTCCGCCCTATACCGACGTCGTCAGCCTGGATTTCGAGGATTATCCCTTCGATATCCAGCGCTTCGACAAGCCC
TGCGCCCTCTGCGGCGGCGAGGATGTCTATCTCGACGAAGTGGTTCTCGACGACAGGGGCGGACGCATGTTCGTCTGCTC
CGACACCGATCATTGCGAAGACCGCCGCGCCCACGGGCATGCCGGTGAAATGCTGGCCCGGGAGGCTGCAGAATGA

Upstream 100 bases:

>100_bases
ATTATGTGGACTTCCAAGCCGAACTCGATCTCGTCCGCCGCATGCGCCGTGAATTCGAAGCCGCCCGCAGCAGCGGCGAA
GATATGAAGGAAGCCGCCGA

Downstream 100 bases:

>100_bases
GCGATATTCCGCTTCTCAAAGTCCACGACGTTTCGAAATTCTACGGCAACCGGATCGGCTGCCGCGACGTTTCCTTCGAG
CTCTGGCCGGGCGAAGTGCT

Product: phosphonate metabolism PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSDLASYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTASIIGPDDVLKVIDQGADDTTNAV
SIRAFFQKVANVAVTTRTSDATIIQTRHRIPEEKLKDGQVLVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYED
IAHNGRISRTYAYPVKVHGRYVMDPSPTPKFDNPKMHMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDYPFDIQRFDKP
CALCGGEDVYLDEVVLDDRGGRMFVCSDTDHCEDRRAHGHAGEMLAREAAE

Sequences:

>Translated_291_residues
MSDLASYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTASIIGPDDVLKVIDQGADDTTNAV
SIRAFFQKVANVAVTTRTSDATIIQTRHRIPEEKLKDGQVLVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYED
IAHNGRISRTYAYPVKVHGRYVMDPSPTPKFDNPKMHMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDYPFDIQRFDKP
CALCGGEDVYLDEVVLDDRGGRMFVCSDTDHCEDRRAHGHAGEMLAREAAE
>Mature_290_residues
SDLASYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTASIIGPDDVLKVIDQGADDTTNAVS
IRAFFQKVANVAVTTRTSDATIIQTRHRIPEEKLKDGQVLVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYEDI
AHNGRISRTYAYPVKVHGRYVMDPSPTPKFDNPKMHMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDYPFDIQRFDKPC
ALCGGEDVYLDEVVLDDRGGRMFVCSDTDHCEDRRAHGHAGEMLAREAAE

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=272, Percent_Identity=76.8382352941177, Blast_Score=450, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 32892; Mature: 32761

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLASYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTASII
CCCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
GPDDVLKVIDQGADDTTNAVSIRAFFQKVANVAVTTRTSDATIIQTRHRIPEEKLKDGQV
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCEEEEECCCCCEEEHHHHCCCHHHCCCCCE
LVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYEDIAHNGRISRTYAYPVKVHGR
EEEECCCCCHHHHCCCCCHHHHHHHHHHHHCCEEEEEEHHHHCCCCEEEEEEEEEEECCE
YVMDPSPTPKFDNPKMHMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDYPFDIQRFDKP
EEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCHHHHCCCC
CALCGGEDVYLDEVVLDDRGGRMFVCSDTDHCEDRRAHGHAGEMLAREAAE
CEECCCCCEEEEEEEEECCCCEEEEECCCCCCHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SDLASYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTASII
CCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEE
GPDDVLKVIDQGADDTTNAVSIRAFFQKVANVAVTTRTSDATIIQTRHRIPEEKLKDGQV
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCEEEEECCCCCEEEHHHHCCCHHHCCCCCE
LVYQVPIPEPLRFLEPRETETRKMHALEEYGLMHVKLYEDIAHNGRISRTYAYPVKVHGR
EEEECCCCCHHHHCCCCCHHHHHHHHHHHHCCEEEEEEHHHHCCCCEEEEEEEEEEECCE
YVMDPSPTPKFDNPKMHMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDYPFDIQRFDKP
EEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCHHHHCCCC
CALCGGEDVYLDEVVLDDRGGRMFVCSDTDHCEDRRAHGHAGEMLAREAAE
CEECCCCCEEEEEEEEECCCCEEEEECCCCCCHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]