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The map label for this gene is rpoH2 [H]

Identifier: 209551384

GI number: 209551384

Start: 3947056

End: 3947919

Strand: Reverse

Name: rpoH2 [H]

Synonym: Rleg2_3813

Alternate gene names: 209551384

Gene position: 3947919-3947056 (Counterclockwise)

Preceding gene: 209551385

Following gene: 209551381

Centisome position: 87.0

GC content: 63.31

Gene sequence:

>864_bases
ATGAAGAACATGTCTGCAGACCGGCGCATGATCAAGATCGCGATGGCCGCCCCCTATCTCGCCCGCCAGGAAGAGCACGA
TCTCGCCATCCGCTGGAAGGATCACGACGACCGCGGCGCGCGCAACCAGATCGCCATGGCCCATATGCGGCTCGTCATAT
CGATGGCGGGCAAGTTCCGCAATTTCGGCCTGCCGATGAGCGATCTCGTGCAGGAGGGCTATGTCGGCCTGCTGGAGGCC
GCCGCCCGCTTCGAGCCGGAACGGGACGTGCGCTTTTCGACCTATGCAAGCTGGTGGATCAGGGCTTCGATCCAGGATTA
TATCCTGCGCAACTGGTCGATCGTGCGCGGCGGCACGAGCTCGGCGCAAAAGGCGCTGTTCTTCAATCTTCGTCGTCTGC
GCGCCAAGCTCGCCAAGGGCGACACACAGCTGACGCTGCAATCCATTCACCAGGAAATCGCCGCGGCGCTCGGCGTCAGC
CTCGCCGATGTGCAGACGATGGATGCCAGGCTTTCCGGCAACGACGCCTCGCTGCAGGCGCCTTCGGTCTCCGGCGATGC
CGAGAGTGCGGAGAAGATGGACTTCCTCGTCAGCGACGATCCCCTGCCGGACGAGCAGGTTTCCAACATGATCGATGGCG
AGCGCCGCCGCGTCTGGCTCGCCTCGGCGCTGAAACATCTCAACGAACGCGAGATGAAAATCATCAGCGCCCGGCGTCTG
GCGGAAGACGGTGCCACGCTCGAAGAACTCGGCGCCGATCTCGGTATTTCCAAGGAGCGTGTGCGGCAGATCGAAAGCCG
GGCGATGGAGAAGCTCCGCAGCGCGCTGGTGAGCGCCGATCCGCATATGGCGGCCTATGCTTAG

Upstream 100 bases:

>100_bases
CCTTTATGCATTCAGAACCCCATTCAGTCCGGACAGCGATGTTCCGGAAGGTGCGTTTTATTGTTCATTTAGGGCGAGAG
GCCTGTTTCAGGAGATCGAA

Downstream 100 bases:

>100_bases
ATTTAAAGTTCTACTGCGTCCCGAGGGCGTCGAAAGACGCCCGGCGCGGTAACTTCGTTCCCTCCAGCAGCACTGACTCG
CCCGGCGTAAGTCGGGTTTT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKNMSADRRMIKIAMAAPYLARQEEHDLAIRWKDHDDRGARNQIAMAHMRLVISMAGKFRNFGLPMSDLVQEGYVGLLEA
AARFEPERDVRFSTYASWWIRASIQDYILRNWSIVRGGTSSAQKALFFNLRRLRAKLAKGDTQLTLQSIHQEIAAALGVS
LADVQTMDARLSGNDASLQAPSVSGDAESAEKMDFLVSDDPLPDEQVSNMIDGERRRVWLASALKHLNEREMKIISARRL
AEDGATLEELGADLGISKERVRQIESRAMEKLRSALVSADPHMAAYA

Sequences:

>Translated_287_residues
MKNMSADRRMIKIAMAAPYLARQEEHDLAIRWKDHDDRGARNQIAMAHMRLVISMAGKFRNFGLPMSDLVQEGYVGLLEA
AARFEPERDVRFSTYASWWIRASIQDYILRNWSIVRGGTSSAQKALFFNLRRLRAKLAKGDTQLTLQSIHQEIAAALGVS
LADVQTMDARLSGNDASLQAPSVSGDAESAEKMDFLVSDDPLPDEQVSNMIDGERRRVWLASALKHLNEREMKIISARRL
AEDGATLEELGADLGISKERVRQIESRAMEKLRSALVSADPHMAAYA
>Mature_287_residues
MKNMSADRRMIKIAMAAPYLARQEEHDLAIRWKDHDDRGARNQIAMAHMRLVISMAGKFRNFGLPMSDLVQEGYVGLLEA
AARFEPERDVRFSTYASWWIRASIQDYILRNWSIVRGGTSSAQKALFFNLRRLRAKLAKGDTQLTLQSIHQEIAAALGVS
LADVQTMDARLSGNDASLQAPSVSGDAESAEKMDFLVSDDPLPDEQVSNMIDGERRRVWLASALKHLNEREMKIISARRL
AEDGATLEELGADLGISKERVRQIESRAMEKLRSALVSADPHMAAYA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=274, Percent_Identity=37.2262773722628, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789098, Length=272, Percent_Identity=32.3529411764706, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=30.672268907563, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=28.110599078341, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32143; Mature: 32143

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMSADRRMIKIAMAAPYLARQEEHDLAIRWKDHDDRGARNQIAMAHMRLVISMAGKFR
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
NFGLPMSDLVQEGYVGLLEAAARFEPERDVRFSTYASWWIRASIQDYILRNWSIVRGGTS
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCC
SAQKALFFNLRRLRAKLAKGDTQLTLQSIHQEIAAALGVSLADVQTMDARLSGNDASLQA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
PSVSGDAESAEKMDFLVSDDPLPDEQVSNMIDGERRRVWLASALKHLNEREMKIISARRL
CCCCCCCHHHHHHHEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEDGATLEELGADLGISKERVRQIESRAMEKLRSALVSADPHMAAYA
HHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure
MKNMSADRRMIKIAMAAPYLARQEEHDLAIRWKDHDDRGARNQIAMAHMRLVISMAGKFR
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
NFGLPMSDLVQEGYVGLLEAAARFEPERDVRFSTYASWWIRASIQDYILRNWSIVRGGTS
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCC
SAQKALFFNLRRLRAKLAKGDTQLTLQSIHQEIAAALGVSLADVQTMDARLSGNDASLQA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
PSVSGDAESAEKMDFLVSDDPLPDEQVSNMIDGERRRVWLASALKHLNEREMKIISARRL
CCCCCCCHHHHHHHEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEDGATLEELGADLGISKERVRQIESRAMEKLRSALVSADPHMAAYA
HHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]