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The map label for this gene is dgoD [H]

Identifier: 209551244

GI number: 209551244

Start: 3801328

End: 3802689

Strand: Direct

Name: dgoD [H]

Synonym: Rleg2_3672

Alternate gene names: 209551244

Gene position: 3801328-3802689 (Clockwise)

Preceding gene: 209551243

Following gene: 209551245

Centisome position: 83.77

GC content: 60.65

Gene sequence:

>1362_bases
ATGCCAGAGGCAGATGGAGCCGACGAGGCGCTCAATCGGGTGAACACGCATTCCAAACCGTCCGACCTTCGCATCACCGA
CATGCGGGTCGCCGAAATCGTCGGCGCGCCGTTCACCACGGTGCTGCTTAAGATTTACACCAACCAGGGCATTGTCGGGC
TTGGCGAGGTGCGCGACGGCGCCAGCGCCACCTATGCGCTGATGCTGAAGAGCCGGTTGCTTGGCGAGAACCCTTGCAAC
ATCGACCGACTGTTTCGCCGGATCAAGCAGTTCGGCGGGCACGGCCGGCAAGGCGGCGGCGTCTCGGCGATTGAAATCGC
GTTGTGGGATCTTGCCGGCAAGGCCTATGGCGTCCCGATCTACCAGATGCTCGGCGGCCGTTTTCGGGAGAAAGTGCGCG
TCTACTGCGATACCGACGCCACCGTGCCGAGCGGCACCGAGACCGGTAAGCGCCTCAAGCAGCGCATGGAACTCGGCTTC
ACCTTTCTCAAAATGGATTTGGGATTGATGCAGATCGCGGATGTGCCCGGCGCGGTCGTCTTTCCTGCCGGCTCATTGGA
AGGATACCGCCATCCTCCTAATCGCGGGCCGCTGAAGACCATTGAAGAGCGGCTTGCCCGCAACGCTGCCTACGATTTGC
ATAACGTCCAGCACCCGTTCACCGGCCTTCAGTTTTCCGACAAGGGCCTCGACCTGCTGGAACAATACATCGCCGAGGTT
CGTGAGGTCATCGGCATGGATGTGCCGCTTGCGATCGACCATATCGGCCATATCTCGATGCAGAACGGCATTCGCCTTGC
CAGGCGAATTGAAAAATACGTGCCGGCCTGGCTTGAGGATGTGATCCCATGGCAGCATACCGAGCAATACCGACAACTGC
AGGACGCCACTACGGTGCCGATCTGCACCGGCGAGGATATCTACCTCAAGGAAGGCTTCGAGCCTCTGCTCAAAAGCGGC
GGCGTCTCCGTTATCCACCCCGACCTGCTCACCAGTGGGGGCATCCTCGAGACCAAGAAGATCGGCGATATGGCGCAGGA
CCGCGGTGTCGCCATGGCCATCCATATGGCGGAAAGTCCGATCGCAGCAATGGCCGCGGCACATGTTGCAACCGCGACCG
AAAATTTCATGGCGCTCGAATACCATTCGGCCGATGTCGACTGGTGGGACGATATCGTCACCGGCCTTCCGAAGCCGCTC
GTGCAAAACGGCTTCATCACTGTTCCCGACAAGCCGGGCCTCGGGATCGACGACGTCGTCGACGAGGTGATCTCGCAGCA
TCTGCAGCCGGGCGTCACCGGCATCTGGCAGCATACCGACCACTGGGACGATGAGTATTCCTGGGACCGCACCTGGAGCT
GA

Upstream 100 bases:

>100_bases
GACTACCTGCAAAACCACGAGATCGAGCTGGACTAGCAACGCTCTCCGCCGCCCCAGCTTGCGCGGCGGCGAGCCCGCCC
GACCAAAACGAGGATAAATC

Downstream 100 bases:

>100_bases
GGTGAAAAGGAACTGAAGATGAAGATCACCGATCTCCGCTGTGCCGTCATCGGCAAACACCCGATCGTCCGCATCGTCAC
GGACGAGGGCCTCTACGGCT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MPEADGADEALNRVNTHSKPSDLRITDMRVAEIVGAPFTTVLLKIYTNQGIVGLGEVRDGASATYALMLKSRLLGENPCN
IDRLFRRIKQFGGHGRQGGGVSAIEIALWDLAGKAYGVPIYQMLGGRFREKVRVYCDTDATVPSGTETGKRLKQRMELGF
TFLKMDLGLMQIADVPGAVVFPAGSLEGYRHPPNRGPLKTIEERLARNAAYDLHNVQHPFTGLQFSDKGLDLLEQYIAEV
REVIGMDVPLAIDHIGHISMQNGIRLARRIEKYVPAWLEDVIPWQHTEQYRQLQDATTVPICTGEDIYLKEGFEPLLKSG
GVSVIHPDLLTSGGILETKKIGDMAQDRGVAMAIHMAESPIAAMAAAHVATATENFMALEYHSADVDWWDDIVTGLPKPL
VQNGFITVPDKPGLGIDDVVDEVISQHLQPGVTGIWQHTDHWDDEYSWDRTWS

Sequences:

>Translated_453_residues
MPEADGADEALNRVNTHSKPSDLRITDMRVAEIVGAPFTTVLLKIYTNQGIVGLGEVRDGASATYALMLKSRLLGENPCN
IDRLFRRIKQFGGHGRQGGGVSAIEIALWDLAGKAYGVPIYQMLGGRFREKVRVYCDTDATVPSGTETGKRLKQRMELGF
TFLKMDLGLMQIADVPGAVVFPAGSLEGYRHPPNRGPLKTIEERLARNAAYDLHNVQHPFTGLQFSDKGLDLLEQYIAEV
REVIGMDVPLAIDHIGHISMQNGIRLARRIEKYVPAWLEDVIPWQHTEQYRQLQDATTVPICTGEDIYLKEGFEPLLKSG
GVSVIHPDLLTSGGILETKKIGDMAQDRGVAMAIHMAESPIAAMAAAHVATATENFMALEYHSADVDWWDDIVTGLPKPL
VQNGFITVPDKPGLGIDDVVDEVISQHLQPGVTGIWQHTDHWDDEYSWDRTWS
>Mature_452_residues
PEADGADEALNRVNTHSKPSDLRITDMRVAEIVGAPFTTVLLKIYTNQGIVGLGEVRDGASATYALMLKSRLLGENPCNI
DRLFRRIKQFGGHGRQGGGVSAIEIALWDLAGKAYGVPIYQMLGGRFREKVRVYCDTDATVPSGTETGKRLKQRMELGFT
FLKMDLGLMQIADVPGAVVFPAGSLEGYRHPPNRGPLKTIEERLARNAAYDLHNVQHPFTGLQFSDKGLDLLEQYIAEVR
EVIGMDVPLAIDHIGHISMQNGIRLARRIEKYVPAWLEDVIPWQHTEQYRQLQDATTVPICTGEDIYLKEGFEPLLKSGG
VSVIHPDLLTSGGILETKKIGDMAQDRGVAMAIHMAESPIAAMAAAHVATATENFMALEYHSADVDWWDDIVTGLPKPLV
QNGFITVPDKPGLGIDDVVDEVISQHLQPGVTGIWQHTDHWDDEYSWDRTWS

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=425, Percent_Identity=26.1176470588235, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1787864, Length=389, Percent_Identity=28.0205655526992, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 50085; Mature: 49954

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEADGADEALNRVNTHSKPSDLRITDMRVAEIVGAPFTTVLLKIYTNQGIVGLGEVRDG
CCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCC
ASATYALMLKSRLLGENPCNIDRLFRRIKQFGGHGRQGGGVSAIEIALWDLAGKAYGVPI
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
YQMLGGRFREKVRVYCDTDATVPSGTETGKRLKQRMELGFTFLKMDLGLMQIADVPGAVV
HHHHCHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEE
FPAGSLEGYRHPPNRGPLKTIEERLARNAAYDLHNVQHPFTGLQFSDKGLDLLEQYIAEV
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
REVIGMDVPLAIDHIGHISMQNGIRLARRIEKYVPAWLEDVIPWQHTEQYRQLQDATTVP
HHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCE
ICTGEDIYLKEGFEPLLKSGGVSVIHPDLLTSGGILETKKIGDMAQDRGVAMAIHMAESP
EECCCCEEHHCCCHHHHHCCCCEEECHHHHCCCCCEEHHHHHHHHHCCCEEEEEEECCCC
IAAMAAAHVATATENFMALEYHSADVDWWDDIVTGLPKPLVQNGFITVPDKPGLGIDDVV
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCHHHHHCCEEECCCCCCCCHHHHH
DEVISQHLQPGVTGIWQHTDHWDDEYSWDRTWS
HHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PEADGADEALNRVNTHSKPSDLRITDMRVAEIVGAPFTTVLLKIYTNQGIVGLGEVRDG
CCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCCC
ASATYALMLKSRLLGENPCNIDRLFRRIKQFGGHGRQGGGVSAIEIALWDLAGKAYGVPI
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
YQMLGGRFREKVRVYCDTDATVPSGTETGKRLKQRMELGFTFLKMDLGLMQIADVPGAVV
HHHHCHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEE
FPAGSLEGYRHPPNRGPLKTIEERLARNAAYDLHNVQHPFTGLQFSDKGLDLLEQYIAEV
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
REVIGMDVPLAIDHIGHISMQNGIRLARRIEKYVPAWLEDVIPWQHTEQYRQLQDATTVP
HHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCE
ICTGEDIYLKEGFEPLLKSGGVSVIHPDLLTSGGILETKKIGDMAQDRGVAMAIHMAESP
EECCCCEEHHCCCHHHHHCCCCEEECHHHHCCCCCEEHHHHHHHHHCCCEEEEEEECCCC
IAAMAAAHVATATENFMALEYHSADVDWWDDIVTGLPKPLVQNGFITVPDKPGLGIDDVV
HHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCHHHHHCCEEECCCCCCCCHHHHH
DEVISQHLQPGVTGIWQHTDHWDDEYSWDRTWS
HHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA