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The map label for this gene is dgoD [H]

Identifier: 209551243

GI number: 209551243

Start: 3800097

End: 3801263

Strand: Direct

Name: dgoD [H]

Synonym: Rleg2_3671

Alternate gene names: 209551243

Gene position: 3800097-3801263 (Clockwise)

Preceding gene: 209551242

Following gene: 209551244

Centisome position: 83.74

GC content: 59.9

Gene sequence:

>1167_bases
ATGAAAATCACCAGCGTTCGGCCGTGGCTGATCAAGTCCGATGCTTCTTATTGGGGAGAGTTTCTGTTCGTCGAAGTGAC
AACCGATGAGGGGGTGAGCGGCTGGGGAGAAATCACCACCACGACAAGGCTCGCCAATCGCGCCCTCTGCACGATCCTGC
GGCAGATCGGTGCTGCTGTGACAGGCGAGGACCCGGCGCGTATCGAGTATCTGTGGCACAAGATATTCCGCAGCTTCACC
TACATGGGCAGTCGCGGCGCCGCAGTCGAATGCGTCAGCGCCATCGATATCGCCCTTTGGGATATCCGGGGCAAAGTTCT
TGGCAAGCCGATTTACGAGCTGTTGGGCGGACCGGTACGCGATGAAATTGCGCTCTACACCCACCCCAACCAGGCCAAAT
TTACCAGCAATGAGGCGGTGATCCGGGAAATTCGAGACATCGTCGAGTCCGGCCACACGGCGCTCAAGTTCGATCCTTTC
CCCCACCAAGGCCGCACCGCCGATGGACAGGCTCGCGAACAGCGGGACGGCTACCTCGATGGCAGCATGACCCGCAAGGA
TGAGCGCGAGGCCGCCGAACTGACCGCCCTCATCCGCGAAACCGCGGGCCCTGATGTCGACATCCTCATCGATGCGCATG
GCCGGTTCGACGTGCCCACCGCCATTCGCCTTTGTCGGAGCCTTGAGGAAGCTGGTCAGATCGACTGGTTCGAGGAGCCT
TGTCCACCGGAGAGCCTCAACGCGCTCAGACAGGTCCGTGAAAAGGTCAGCGCCGCTATCTCGTGGGGCGAGCGCGGCCA
CACGAAGTGGGATTTCGTGCCGGTGCTCGAAAACAGGCTCGCTGACTACATCATGCCTGACGTCACCTGGACCGGCGGCA
TTACTGAACTGAAGAAGATTTCCGCCCTATGCGAGGCCTACTACATCCCGGTCTCGCCGCATGACGCCGCAGGGCCGATC
AACGTGGTTGCGGGCGCGCAAGTGATGATGACCGTTCCCAACTTCTACAAACTCGAAACGTCGGAGTGGAACCTGGGCAA
ATACGATCACCTCATCGACAGACCGCTCGATGTTTCGAACGGCAGCCTCAAGCTTACGCCCAAGCCTGGTCTCGGCGTCG
AAATGAACCGCGACTACCTGCAAAACCACGAGATCGAGCTGGACTAG

Upstream 100 bases:

>100_bases
CATCCTGGAACACATGCGTGCGCGGAACGCCGCTCGCGCAGGATAGAAGCCATCGCCCGGAATATCGTTATCCCGACCAC
TTCAGACAAAGGCAGTTGAG

Downstream 100 bases:

>100_bases
CAACGCTCTCCGCCGCCCCAGCTTGCGCGGCGGCGAGCCCGCCCGACCAAAACGAGGATAAATCATGCCAGAGGCAGATG
GAGCCGACGAGGCGCTCAAT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKITSVRPWLIKSDASYWGEFLFVEVTTDEGVSGWGEITTTTRLANRALCTILRQIGAAVTGEDPARIEYLWHKIFRSFT
YMGSRGAAVECVSAIDIALWDIRGKVLGKPIYELLGGPVRDEIALYTHPNQAKFTSNEAVIREIRDIVESGHTALKFDPF
PHQGRTADGQAREQRDGYLDGSMTRKDEREAAELTALIRETAGPDVDILIDAHGRFDVPTAIRLCRSLEEAGQIDWFEEP
CPPESLNALRQVREKVSAAISWGERGHTKWDFVPVLENRLADYIMPDVTWTGGITELKKISALCEAYYIPVSPHDAAGPI
NVVAGAQVMMTVPNFYKLETSEWNLGKYDHLIDRPLDVSNGSLKLTPKPGLGVEMNRDYLQNHEIELD

Sequences:

>Translated_388_residues
MKITSVRPWLIKSDASYWGEFLFVEVTTDEGVSGWGEITTTTRLANRALCTILRQIGAAVTGEDPARIEYLWHKIFRSFT
YMGSRGAAVECVSAIDIALWDIRGKVLGKPIYELLGGPVRDEIALYTHPNQAKFTSNEAVIREIRDIVESGHTALKFDPF
PHQGRTADGQAREQRDGYLDGSMTRKDEREAAELTALIRETAGPDVDILIDAHGRFDVPTAIRLCRSLEEAGQIDWFEEP
CPPESLNALRQVREKVSAAISWGERGHTKWDFVPVLENRLADYIMPDVTWTGGITELKKISALCEAYYIPVSPHDAAGPI
NVVAGAQVMMTVPNFYKLETSEWNLGKYDHLIDRPLDVSNGSLKLTPKPGLGVEMNRDYLQNHEIELD
>Mature_388_residues
MKITSVRPWLIKSDASYWGEFLFVEVTTDEGVSGWGEITTTTRLANRALCTILRQIGAAVTGEDPARIEYLWHKIFRSFT
YMGSRGAAVECVSAIDIALWDIRGKVLGKPIYELLGGPVRDEIALYTHPNQAKFTSNEAVIREIRDIVESGHTALKFDPF
PHQGRTADGQAREQRDGYLDGSMTRKDEREAAELTALIRETAGPDVDILIDAHGRFDVPTAIRLCRSLEEAGQIDWFEEP
CPPESLNALRQVREKVSAAISWGERGHTKWDFVPVLENRLADYIMPDVTWTGGITELKKISALCEAYYIPVSPHDAAGPI
NVVAGAQVMMTVPNFYKLETSEWNLGKYDHLIDRPLDVSNGSLKLTPKPGLGVEMNRDYLQNHEIELD

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=384, Percent_Identity=31.25, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787864, Length=374, Percent_Identity=26.4705882352941, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI226510960, Length=374, Percent_Identity=28.0748663101604, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 43430; Mature: 43430

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITSVRPWLIKSDASYWGEFLFVEVTTDEGVSGWGEITTTTRLANRALCTILRQIGAAV
CCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TGEDPARIEYLWHKIFRSFTYMGSRGAAVECVSAIDIALWDIRGKVLGKPIYELLGGPVR
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHCCCCCC
DEIALYTHPNQAKFTSNEAVIREIRDIVESGHTALKFDPFPHQGRTADGQAREQRDGYLD
CCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHCCCCC
GSMTRKDEREAAELTALIRETAGPDVDILIDAHGRFDVPTAIRLCRSLEEAGQIDWFEEP
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCC
CPPESLNALRQVREKVSAAISWGERGHTKWDFVPVLENRLADYIMPDVTWTGGITELKKI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHH
SALCEAYYIPVSPHDAAGPINVVAGAQVMMTVPNFYKLETSEWNLGKYDHLIDRPLDVSN
HHHHHHEEECCCCCCCCCCEEEEECCEEEEECCCEEEEECCCCCCCCHHHHHCCCCCCCC
GSLKLTPKPGLGVEMNRDYLQNHEIELD
CEEEECCCCCCCCEECHHHHHCCCCCCC
>Mature Secondary Structure
MKITSVRPWLIKSDASYWGEFLFVEVTTDEGVSGWGEITTTTRLANRALCTILRQIGAAV
CCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TGEDPARIEYLWHKIFRSFTYMGSRGAAVECVSAIDIALWDIRGKVLGKPIYELLGGPVR
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHCCCCCC
DEIALYTHPNQAKFTSNEAVIREIRDIVESGHTALKFDPFPHQGRTADGQAREQRDGYLD
CCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHCCCCC
GSMTRKDEREAAELTALIRETAGPDVDILIDAHGRFDVPTAIRLCRSLEEAGQIDWFEEP
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCC
CPPESLNALRQVREKVSAAISWGERGHTKWDFVPVLENRLADYIMPDVTWTGGITELKKI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHH
SALCEAYYIPVSPHDAAGPINVVAGAQVMMTVPNFYKLETSEWNLGKYDHLIDRPLDVSN
HHHHHHEEECCCCCCCCCCEEEEECCEEEEECCCEEEEECCCCCCCCHHHHHCCCCCCCC
GSLKLTPKPGLGVEMNRDYLQNHEIELD
CEEEECCCCCCCCEECHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]