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The map label for this gene is dgoD [H]

Identifier: 209551245

GI number: 209551245

Start: 3802708

End: 3803907

Strand: Direct

Name: dgoD [H]

Synonym: Rleg2_3673

Alternate gene names: 209551245

Gene position: 3802708-3803907 (Clockwise)

Preceding gene: 209551244

Following gene: 209551265

Centisome position: 83.8

GC content: 60.33

Gene sequence:

>1200_bases
ATGAAGATCACCGATCTCCGCTGTGCCGTCATCGGCAAACACCCGATCGTCCGCATCGTCACGGACGAGGGCCTCTACGG
CTTGGGCGAAGTCGAATTCACCAAGTCCTATCTGAAGCCCTGGGTACTGCATTTTCGTGACGCGCTGATCGGCGAGGACC
CGACCGACGTCGAGCGCGTCATGCTGAAGATCCGCCAACGCGGCTCTTTCAAGCCCTATGGCGCGGCGGTGAGCGCGATT
GAGCATGCGCTGTGGGATATTGCCGGCAAGGCCGCCGGTGTGCCCGCCTATAAACTGCTCGGCGGCAAGGTGCGTGACAA
GGTGCGCGTCTATAACGGCTCTGTTCGCCGGAAACGCACGGGCGACCGGCCGGAGGATTACGCCGCCGACGTCAAGTGGA
TGATGGAGCAGCCGCAGAACTTCTTCATGATCAAGCAAGGCATCTCGTTCCACTCCAACATGAAGGACACCATCGAAGAC
TTCCACTACGGCGTGACGCAGAAGAAGGCTGGCTATCACGGCGCCATGGATCAGGGCGTGATCAGCGAGCGCTGTTTCAA
TCACATGCTCGACTGCGTGATCGCCATGAAGGAAGTGCTGGGCGACAAAGTCAGCCTGGCGCTCGACTGCGGCCCCGGCT
GGATGCTGCCCGACGCGATCAAATTCGCCCGCGCGGTCGAAAAGTACAATTTGATGTGGCTCGAGGATATGCTGACTGGC
GACTACGTGCCCTGGGTCAATCCGCAGGCCTATCGGGAACTGACAACCTCCACCTCGACGCCCATCCACACTGGTGAGCA
GATCTATCTGCGGCACAATTTCAAGGAACTGATCGAGACGCAGGCGGTCCGCGTCATCGGCCCCGATCCAGCCGATATCG
GCGGCATTGCCGAGCTCAAATGGGTCGCCGAGCACGCCTACATGCACTCGATCCTGATGGCACCGCACGGCACTGCCAAC
GGCCTGCTGGGCCTCGGCGCGTTGATCAATGTCTGCGCCACGTTGCCGGCAAATTACGTCGCGTTCGAATATCCGAGCGC
CTCCGACCCCTGGTGGGAGGATCTGGTAATCGGATTGCCGCCGCAGATCGTGAAGGACAGCATGGTGGACCTTCTGGAAG
CGCCGGGGCTGGGCCTCGATATCGACGCTGAAGCGGCCAGGAAATATCTGAGCGAAGAGGATGTGGACTTTTTCGACTGA

Upstream 100 bases:

>100_bases
TCTGCAGCCGGGCGTCACCGGCATCTGGCAGCATACCGACCACTGGGACGATGAGTATTCCTGGGACCGCACCTGGAGCT
GAGGTGAAAAGGAACTGAAG

Downstream 100 bases:

>100_bases
ACCGCCCTGCGCCTGTCGCGGCCAGCTTGTCGGGCGCCAGGCAATAGAACCAAAACAGCCGCCTGCCTCGCGGCAAGCGG
CTGAATGCTTTTTTACGAAT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKITDLRCAVIGKHPIVRIVTDEGLYGLGEVEFTKSYLKPWVLHFRDALIGEDPTDVERVMLKIRQRGSFKPYGAAVSAI
EHALWDIAGKAAGVPAYKLLGGKVRDKVRVYNGSVRRKRTGDRPEDYAADVKWMMEQPQNFFMIKQGISFHSNMKDTIED
FHYGVTQKKAGYHGAMDQGVISERCFNHMLDCVIAMKEVLGDKVSLALDCGPGWMLPDAIKFARAVEKYNLMWLEDMLTG
DYVPWVNPQAYRELTTSTSTPIHTGEQIYLRHNFKELIETQAVRVIGPDPADIGGIAELKWVAEHAYMHSILMAPHGTAN
GLLGLGALINVCATLPANYVAFEYPSASDPWWEDLVIGLPPQIVKDSMVDLLEAPGLGLDIDAEAARKYLSEEDVDFFD

Sequences:

>Translated_399_residues
MKITDLRCAVIGKHPIVRIVTDEGLYGLGEVEFTKSYLKPWVLHFRDALIGEDPTDVERVMLKIRQRGSFKPYGAAVSAI
EHALWDIAGKAAGVPAYKLLGGKVRDKVRVYNGSVRRKRTGDRPEDYAADVKWMMEQPQNFFMIKQGISFHSNMKDTIED
FHYGVTQKKAGYHGAMDQGVISERCFNHMLDCVIAMKEVLGDKVSLALDCGPGWMLPDAIKFARAVEKYNLMWLEDMLTG
DYVPWVNPQAYRELTTSTSTPIHTGEQIYLRHNFKELIETQAVRVIGPDPADIGGIAELKWVAEHAYMHSILMAPHGTAN
GLLGLGALINVCATLPANYVAFEYPSASDPWWEDLVIGLPPQIVKDSMVDLLEAPGLGLDIDAEAARKYLSEEDVDFFD
>Mature_399_residues
MKITDLRCAVIGKHPIVRIVTDEGLYGLGEVEFTKSYLKPWVLHFRDALIGEDPTDVERVMLKIRQRGSFKPYGAAVSAI
EHALWDIAGKAAGVPAYKLLGGKVRDKVRVYNGSVRRKRTGDRPEDYAADVKWMMEQPQNFFMIKQGISFHSNMKDTIED
FHYGVTQKKAGYHGAMDQGVISERCFNHMLDCVIAMKEVLGDKVSLALDCGPGWMLPDAIKFARAVEKYNLMWLEDMLTG
DYVPWVNPQAYRELTTSTSTPIHTGEQIYLRHNFKELIETQAVRVIGPDPADIGGIAELKWVAEHAYMHSILMAPHGTAN
GLLGLGALINVCATLPANYVAFEYPSASDPWWEDLVIGLPPQIVKDSMVDLLEAPGLGLDIDAEAARKYLSEEDVDFFD

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=379, Percent_Identity=25.8575197889182, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787864, Length=395, Percent_Identity=26.8354430379747, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI226510960, Length=320, Percent_Identity=26.5625, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44606; Mature: 44606

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITDLRCAVIGKHPIVRIVTDEGLYGLGEVEFTKSYLKPWVLHFRDALIGEDPTDVERV
CCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MLKIRQRGSFKPYGAAVSAIEHALWDIAGKAAGVPAYKLLGGKVRDKVRVYNGSVRRKRT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHEEEECCCCHHCCC
GDRPEDYAADVKWMMEQPQNFFMIKQGISFHSNMKDTIEDFHYGVTQKKAGYHGAMDQGV
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHH
ISERCFNHMLDCVIAMKEVLGDKVSLALDCGPGWMLPDAIKFARAVEKYNLMWLEDMLTG
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHCC
DYVPWVNPQAYRELTTSTSTPIHTGEQIYLRHNFKELIETQAVRVIGPDPADIGGIAELK
CCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHCCEEEECCCCCCCCCHHHHH
WVAEHAYMHSILMAPHGTANGLLGLGALINVCATLPANYVAFEYPSASDPWWEDLVIGLP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHEECCC
PQIVKDSMVDLLEAPGLGLDIDAEAARKYLSEEDVDFFD
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKITDLRCAVIGKHPIVRIVTDEGLYGLGEVEFTKSYLKPWVLHFRDALIGEDPTDVERV
CCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MLKIRQRGSFKPYGAAVSAIEHALWDIAGKAAGVPAYKLLGGKVRDKVRVYNGSVRRKRT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHEEEECCCCHHCCC
GDRPEDYAADVKWMMEQPQNFFMIKQGISFHSNMKDTIEDFHYGVTQKKAGYHGAMDQGV
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHH
ISERCFNHMLDCVIAMKEVLGDKVSLALDCGPGWMLPDAIKFARAVEKYNLMWLEDMLTG
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHCC
DYVPWVNPQAYRELTTSTSTPIHTGEQIYLRHNFKELIETQAVRVIGPDPADIGGIAELK
CCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHCCEEEECCCCCCCCCHHHHH
WVAEHAYMHSILMAPHGTANGLLGLGALINVCATLPANYVAFEYPSASDPWWEDLVIGLP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHEECCC
PQIVKDSMVDLLEAPGLGLDIDAEAARKYLSEEDVDFFD
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA