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The map label for this gene is bigR [H]

Identifier: 209551214

GI number: 209551214

Start: 3771756

End: 3772097

Strand: Reverse

Name: bigR [H]

Synonym: Rleg2_3640

Alternate gene names: 209551214

Gene position: 3772097-3771756 (Counterclockwise)

Preceding gene: 209551215

Following gene: 209551213

Centisome position: 83.12

GC content: 55.26

Gene sequence:

>342_bases
ATGCCGTTGCCTAAACCGGAGCCGGGGATGACCGGCCAGGACTTGCACATACTCGCTGGAAAAGCCCATGAAGCGAGCGA
TCTGCTGAAAGCTTTGGCGCATCACACCCGATTGCTGATCCTCTGCATCCTGGCGAAACAGGAGCGGACGGTCGGTGAAA
TCGAAAATATTCTCGGCATTCAGCAGGCGATGGTGTCGCAGCAGCTTGCGCGTTTGAGGCTCGAAGGCCTGGTCAATACG
CGCCGTCAAGGCAGGCTGGTTTATTACAGTATCGGCAATGTCAGCGTGCTTGCCTTCCTCGAATCGCTGTTCGATCTTTT
TCCCGCTGCGGAAAACTGTTAA

Upstream 100 bases:

>100_bases
ACTTGGACCTTTTTTGGCATTTCATCACTATTGACCAATTCGTTTGTGCGAATATGCTATTGTGCTGATCTACCAAATCG
CCTCCCTTCCGAGATATCGA

Downstream 100 bases:

>100_bases
AGCGCGTCGCCTTAACTTGATTCATTCCCTTGCGGAGCACGGTTGCTGTGTCGAAGATGAGGCCGGACGCGAAATGTCGC
GGGAGACCGGATGATCGACA

Product: ArsR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 113; Mature: 112

Protein sequence:

>113_residues
MPLPKPEPGMTGQDLHILAGKAHEASDLLKALAHHTRLLILCILAKQERTVGEIENILGIQQAMVSQQLARLRLEGLVNT
RRQGRLVYYSIGNVSVLAFLESLFDLFPAAENC

Sequences:

>Translated_113_residues
MPLPKPEPGMTGQDLHILAGKAHEASDLLKALAHHTRLLILCILAKQERTVGEIENILGIQQAMVSQQLARLRLEGLVNT
RRQGRLVYYSIGNVSVLAFLESLFDLFPAAENC
>Mature_112_residues
PLPKPEPGMTGQDLHILAGKAHEASDLLKALAHHTRLLILCILAKQERTVGEIENILGIQQAMVSQQLARLRLEGLVNTR
RQGRLVYYSIGNVSVLAFLESLFDLFPAAENC

Specific function: Represses an operon that comprises itself, XF_0764, XF_0765, XF_0766 and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=95, Percent_Identity=40, Blast_Score=71, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 12486; Mature: 12355

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPKPEPGMTGQDLHILAGKAHEASDLLKALAHHTRLLILCILAKQERTVGEIENILGI
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QQAMVSQQLARLRLEGLVNTRRQGRLVYYSIGNVSVLAFLESLFDLFPAAENC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PLPKPEPGMTGQDLHILAGKAHEASDLLKALAHHTRLLILCILAKQERTVGEIENILGI
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
QQAMVSQQLARLRLEGLVNTRRQGRLVYYSIGNVSVLAFLESLFDLFPAAENC
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]