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The map label for this gene is emrB [H]

Identifier: 209550990

GI number: 209550990

Start: 3529210

End: 3530808

Strand: Reverse

Name: emrB [H]

Synonym: Rleg2_3414

Alternate gene names: 209550990

Gene position: 3530808-3529210 (Counterclockwise)

Preceding gene: 209550991

Following gene: 209550989

Centisome position: 77.81

GC content: 60.29

Gene sequence:

>1599_bases
ATGGCCGGTAGCGCGACAGCAACAGCGGGGACGATTCCGGCAGCGCCTGCCCACGCCGACGAACACATGGATCCGAGGAA
GCTCATCGCCTTCTTCGCGATGGTGCTCGGCATGTTCATGTCGATCCTCGACATCCAGATCGTCTCGGCCTCGCTCGCCG
AAATCCAGGCCGGCCTTTCGGCCGGCAGCGATGAAGTCGGCTGGGTGCAGACGGCCTATCTGATCGCTGAGGTGATCATG
ATCCCGCTGTCGGGCACGCTGGCGCGGATCATTTCGACGCGCTATCTTTTCGCGATCTCGGCCGCCGGTTTCACCATGGC
AAGCGCGCTTGCCGCGACGGCGACGAATATCGACCAGATGATCGTCTACCGCGCCATTCAAGGCTTCATCGGCGGCGGCA
TGATCCCGTCGGTGTTCGCGGCCGCCTTCACCATCTTCCCGGCGTCGAAGCGCAGCATCGTTTCGCCGATCATCGGCCTG
ATCGCGACGCTGGCGCCGACCATCGGCCCGACGGTCGGCGGTTATCTCAGCCACGCCTTCTCCTGGCATTGGCTGTTCCT
CGTCAATATCGTTCCCGGCATCATCGTTACGATCGTCACCTGGAACTTCATCGATTTCGACAAGCCGGAACTGTCGCTCT
TCAAGAAGTTCGACTGGTGGGGACTGATCTCGATGGGCATCTTCCTCGGCACGATGGAATATGTGCTCGAAGAGGGTAAT
TCGAACGACTGGTTCAACGACAGTTCGATCGCGATTGCCGCCGTCGCCTCGGGCGTTGGAGCCATCGTCTTCTTCTATCG
CGCCTTCACGGTGGAGTTTCCCGTCGTCGATCTCAAGGCCTTTGCGAACCGGAATTTCTCATTCGGCTCGATCTTCTCCT
TCGTCATGGGTATCGGCCTCTATGGTCTCACTTATATCTACCCGGTCTATCTCGGCCGCATCCGCGGCTATGATTCGCTG
ATGATCGGCCAGACGATGTTCGTCTCCGGGCTGGCGATGTTCTTCACTGCACCGATCGCCGGGCGGCTGTCGACGAAGAT
GGACCTGCGGCTGATGATGGTGATCGGTTTCATCAGCTTCGCCGCCGGCACCTACATCATGATCCACCTGACGGCGGATT
GGGATTTCTACGAACTGTTCATTCCGCAGATCCTGCGCGGTTTCGGGCTGATGATGTGCATGGTGCCGATCAACAATATC
GCGCTCGGCACGATGCCGCCCTCGCGCATCCGCGGCGCATCCGGCCTGTTCAACCTGACCAGAAACCTCGGCGGCGCCGT
CGGTCTCGCGGTCATCAACACCGTGCTCACCAACCGCCAGGACGTGCATTACGAGCGGCTGCGGGAGAATATGGACTGGG
GCAATCCGGCGGCGATCGACCAGATGAACAACATGACCGCCAATTTCAACACATACGGCATGGACGGCGCCTCGGTCGCG
ATCAAGCAGATGGTCGGCCTTGCCACCAAGCAGGCTGTGATCCTGTCCTTCAGCGACGTGTTCCTGATCCTGACCGCGCT
TTTCGTTGCGATGATCTTCGGTGTCGCGATGATCAAGAAACCCGCGCCGCAAGGCGGGGGAGGCGGTGGCGGCCACTGA

Upstream 100 bases:

>100_bases
CGACAGCGGGCGTCTGCGCGCGGGCCTCAGCGTCGTCGTCGACGTCGATACCCGCACGGCGCCGAGCAAGTAAGCCTTTA
GACAAGGCGGAGGTTCTCCG

Downstream 100 bases:

>100_bases
CCTCCGGCCTGCCGCCCCATGGAAAGGTCCTCTCCGGAGGACCTTTTTATTTGCCGTTTTCCAGCCCTTATGTCATCAGC
ATGTAATCTTCGGTCGGCTT

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MAGSATATAGTIPAAPAHADEHMDPRKLIAFFAMVLGMFMSILDIQIVSASLAEIQAGLSAGSDEVGWVQTAYLIAEVIM
IPLSGTLARIISTRYLFAISAAGFTMASALAATATNIDQMIVYRAIQGFIGGGMIPSVFAAAFTIFPASKRSIVSPIIGL
IATLAPTIGPTVGGYLSHAFSWHWLFLVNIVPGIIVTIVTWNFIDFDKPELSLFKKFDWWGLISMGIFLGTMEYVLEEGN
SNDWFNDSSIAIAAVASGVGAIVFFYRAFTVEFPVVDLKAFANRNFSFGSIFSFVMGIGLYGLTYIYPVYLGRIRGYDSL
MIGQTMFVSGLAMFFTAPIAGRLSTKMDLRLMMVIGFISFAAGTYIMIHLTADWDFYELFIPQILRGFGLMMCMVPINNI
ALGTMPPSRIRGASGLFNLTRNLGGAVGLAVINTVLTNRQDVHYERLRENMDWGNPAAIDQMNNMTANFNTYGMDGASVA
IKQMVGLATKQAVILSFSDVFLILTALFVAMIFGVAMIKKPAPQGGGGGGGH

Sequences:

>Translated_532_residues
MAGSATATAGTIPAAPAHADEHMDPRKLIAFFAMVLGMFMSILDIQIVSASLAEIQAGLSAGSDEVGWVQTAYLIAEVIM
IPLSGTLARIISTRYLFAISAAGFTMASALAATATNIDQMIVYRAIQGFIGGGMIPSVFAAAFTIFPASKRSIVSPIIGL
IATLAPTIGPTVGGYLSHAFSWHWLFLVNIVPGIIVTIVTWNFIDFDKPELSLFKKFDWWGLISMGIFLGTMEYVLEEGN
SNDWFNDSSIAIAAVASGVGAIVFFYRAFTVEFPVVDLKAFANRNFSFGSIFSFVMGIGLYGLTYIYPVYLGRIRGYDSL
MIGQTMFVSGLAMFFTAPIAGRLSTKMDLRLMMVIGFISFAAGTYIMIHLTADWDFYELFIPQILRGFGLMMCMVPINNI
ALGTMPPSRIRGASGLFNLTRNLGGAVGLAVINTVLTNRQDVHYERLRENMDWGNPAAIDQMNNMTANFNTYGMDGASVA
IKQMVGLATKQAVILSFSDVFLILTALFVAMIFGVAMIKKPAPQGGGGGGGH
>Mature_531_residues
AGSATATAGTIPAAPAHADEHMDPRKLIAFFAMVLGMFMSILDIQIVSASLAEIQAGLSAGSDEVGWVQTAYLIAEVIMI
PLSGTLARIISTRYLFAISAAGFTMASALAATATNIDQMIVYRAIQGFIGGGMIPSVFAAAFTIFPASKRSIVSPIIGLI
ATLAPTIGPTVGGYLSHAFSWHWLFLVNIVPGIIVTIVTWNFIDFDKPELSLFKKFDWWGLISMGIFLGTMEYVLEEGNS
NDWFNDSSIAIAAVASGVGAIVFFYRAFTVEFPVVDLKAFANRNFSFGSIFSFVMGIGLYGLTYIYPVYLGRIRGYDSLM
IGQTMFVSGLAMFFTAPIAGRLSTKMDLRLMMVIGFISFAAGTYIMIHLTADWDFYELFIPQILRGFGLMMCMVPINNIA
LGTMPPSRIRGASGLFNLTRNLGGAVGLAVINTVLTNRQDVHYERLRENMDWGNPAAIDQMNNMTANFNTYGMDGASVAI
KQMVGLATKQAVILSFSDVFLILTALFVAMIFGVAMIKKPAPQGGGGGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=492, Percent_Identity=29.0650406504065, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1788710, Length=498, Percent_Identity=28.9156626506024, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI1790195, Length=434, Percent_Identity=23.5023041474654, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081983, Length=428, Percent_Identity=26.1682242990654, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788392, Length=276, Percent_Identity=27.8985507246377, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=135, Percent_Identity=27.4074074074074, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=191, Percent_Identity=25.130890052356, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57328; Mature: 57197

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSATATAGTIPAAPAHADEHMDPRKLIAFFAMVLGMFMSILDIQIVSASLAEIQAGLS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AGSDEVGWVQTAYLIAEVIMIPLSGTLARIISTRYLFAISAAGFTMASALAATATNIDQM
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYRAIQGFIGGGMIPSVFAAAFTIFPASKRSIVSPIIGLIATLAPTIGPTVGGYLSHAF
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SWHWLFLVNIVPGIIVTIVTWNFIDFDKPELSLFKKFDWWGLISMGIFLGTMEYVLEEGN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
SNDWFNDSSIAIAAVASGVGAIVFFYRAFTVEFPVVDLKAFANRNFSFGSIFSFVMGIGL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
YGLTYIYPVYLGRIRGYDSLMIGQTMFVSGLAMFFTAPIAGRLSTKMDLRLMMVIGFISF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHH
AAGTYIMIHLTADWDFYELFIPQILRGFGLMMCMVPINNIALGTMPPSRIRGASGLFNLT
HCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHCCCHHHHHHH
RNLGGAVGLAVINTVLTNRQDVHYERLRENMDWGNPAAIDQMNNMTANFNTYGMDGASVA
HHHCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHH
IKQMVGLATKQAVILSFSDVFLILTALFVAMIFGVAMIKKPAPQGGGGGGGH
HHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGSATATAGTIPAAPAHADEHMDPRKLIAFFAMVLGMFMSILDIQIVSASLAEIQAGLS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC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HHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]