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The map label for this gene is emrK [H]

Identifier: 209550991

GI number: 209550991

Start: 3530836

End: 3532056

Strand: Reverse

Name: emrK [H]

Synonym: Rleg2_3415

Alternate gene names: 209550991

Gene position: 3532056-3530836 (Counterclockwise)

Preceding gene: 209550997

Following gene: 209550990

Centisome position: 77.83

GC content: 64.21

Gene sequence:

>1221_bases
ATGTCGAGCAACCAGAAAAGCAACGTCGCGCGGATTGTCAGCGAAAACGCCGAAGCCGAAGAGGCAAAGGCCGAAGTCGT
GACCGTTGCCGAAGCTCCGATGGCTGAAGCCCTGGAAAAGCCGTCTGCCCCTGCACAGTCGGCGCCGGCATCAGCTCCGG
TCGCTGCACCTGCAGCTCCGAAGAAGCGCCGCAGCCCGGTACTGCCGATCGTCGCTCTCGTCATTCTGGCGGGTGGCGGC
TGGTATGGTTACGAATGGTGGACGAACGGCCGCTTCATGGTGTCGACAGACGACGCCTATATCGAGGGCGATATTGCAAC
CATATCGCCGAAGGTCACGGGCTACGTCGCCAGGGTGAATGTGGTCGCCAACCAGGAAGTCAAAGCGGGCGACGTGCTTG
CCACGCTTGATAACGGCGACTATCAGAACGCCCTCGATCAGGCGCAGGCCCAGATCGCCACCGAACAGCTTTCGCTGCAG
CGCATCGACGCGCAGATCGAAGGCGCCAATGCCAGCCTCGTGCAGGCGCGGGCGCAAAAGGTGGCACTCGAAGCGGCCGT
TCGCGGTGCCGAGATCACTCAGAAGCGCCAGAGCGAGCTGCAGGCGAAGTCGGTCGGCACTGCCGCCGATCTCGACAATG
CCAATATCGCGCTCGACCAGGCCAAGGCCAATCTTGCCGGCGGCGACGCCAACATCACCGCCGCGCAGGCCAACATCACC
ATCCTCCAGGCGCAGCGCAAGGAGGCCGAAGGTTCGGTCCGGTCGCTGGAAATCTCGCGCGACAAGGCCGCCCGCGACCT
CTCCTTCACCGTGCTCAAGGCGCCCTATGACGGCATCGTCGGCAACCGCTCGGTCCAGGAAGGTGATCTGGTTTCGCCCG
GCCAGCGCCTGATGGCGCTCGTTCCGGTGCGGGAGCTCTATATCGACGCCAACTTCAAGGAAACGCAGATCCAGCATCTG
GTTGCGGGCTCGAAGGTCAACGTCCATGTCGATGCCTATAGCGACCATCCGGTCGTCGGCACCGTCGAATCGATCTCGCC
GGCGTCCGGCTCGGTGTTCTCGCTGCTGCCGCCGGAAAATGCGACGGGCAATTTCACCAAGGTGATCCAGCGCGTGCCGG
TGCGCATCGCGCTGCCGCAGGACGCGCTCGACAGCGGGCGTCTGCGCGCGGGCCTCAGCGTCGTCGTCGACGTCGATACC
CGCACGGCGCCGAGCAAGTAA

Upstream 100 bases:

>100_bases
GATTGGTTCTATACATCTTGATATGACGATAAAACAGGTTTAAGTCAACTGAACCGAACCGTTCAGTTCGATTATTTACT
CCAAGATCGGTAAAGTGGTC

Downstream 100 bases:

>100_bases
GCCTTTAGACAAGGCGGAGGTTCTCCGATGGCCGGTAGCGCGACAGCAACAGCGGGGACGATTCCGGCAGCGCCTGCCCA
CGCCGACGAACACATGGATC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSSNQKSNVARIVSENAEAEEAKAEVVTVAEAPMAEALEKPSAPAQSAPASAPVAAPAAPKKRRSPVLPIVALVILAGGG
WYGYEWWTNGRFMVSTDDAYIEGDIATISPKVTGYVARVNVVANQEVKAGDVLATLDNGDYQNALDQAQAQIATEQLSLQ
RIDAQIEGANASLVQARAQKVALEAAVRGAEITQKRQSELQAKSVGTAADLDNANIALDQAKANLAGGDANITAAQANIT
ILQAQRKEAEGSVRSLEISRDKAARDLSFTVLKAPYDGIVGNRSVQEGDLVSPGQRLMALVPVRELYIDANFKETQIQHL
VAGSKVNVHVDAYSDHPVVGTVESISPASGSVFSLLPPENATGNFTKVIQRVPVRIALPQDALDSGRLRAGLSVVVDVDT
RTAPSK

Sequences:

>Translated_406_residues
MSSNQKSNVARIVSENAEAEEAKAEVVTVAEAPMAEALEKPSAPAQSAPASAPVAAPAAPKKRRSPVLPIVALVILAGGG
WYGYEWWTNGRFMVSTDDAYIEGDIATISPKVTGYVARVNVVANQEVKAGDVLATLDNGDYQNALDQAQAQIATEQLSLQ
RIDAQIEGANASLVQARAQKVALEAAVRGAEITQKRQSELQAKSVGTAADLDNANIALDQAKANLAGGDANITAAQANIT
ILQAQRKEAEGSVRSLEISRDKAARDLSFTVLKAPYDGIVGNRSVQEGDLVSPGQRLMALVPVRELYIDANFKETQIQHL
VAGSKVNVHVDAYSDHPVVGTVESISPASGSVFSLLPPENATGNFTKVIQRVPVRIALPQDALDSGRLRAGLSVVVDVDT
RTAPSK
>Mature_405_residues
SSNQKSNVARIVSENAEAEEAKAEVVTVAEAPMAEALEKPSAPAQSAPASAPVAAPAAPKKRRSPVLPIVALVILAGGGW
YGYEWWTNGRFMVSTDDAYIEGDIATISPKVTGYVARVNVVANQEVKAGDVLATLDNGDYQNALDQAQAQIATEQLSLQR
IDAQIEGANASLVQARAQKVALEAAVRGAEITQKRQSELQAKSVGTAADLDNANIALDQAKANLAGGDANITAAQANITI
LQAQRKEAEGSVRSLEISRDKAARDLSFTVLKAPYDGIVGNRSVQEGDLVSPGQRLMALVPVRELYIDANFKETQIQHLV
AGSKVNVHVDAYSDHPVVGTVESISPASGSVFSLLPPENATGNFTKVIQRVPVRIALPQDALDSGRLRAGLSVVVDVDTR
TAPSK

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=341, Percent_Identity=34.6041055718475, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789041, Length=364, Percent_Identity=34.3406593406593, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1790519, Length=325, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87081951, Length=318, Percent_Identity=29.874213836478, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789637, Length=322, Percent_Identity=26.7080745341615, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787013, Length=323, Percent_Identity=27.2445820433437, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790024, Length=248, Percent_Identity=25.4032258064516, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42784; Mature: 42653

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNQKSNVARIVSENAEAEEAKAEVVTVAEAPMAEALEKPSAPAQSAPASAPVAAPAAP
CCCCCHHHHHHHHHCCCCHHHHHEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
KKRRSPVLPIVALVILAGGGWYGYEWWTNGRFMVSTDDAYIEGDIATISPKVTGYVARVN
HHCCCCCHHHHHHHHHCCCCEECEEEEECCEEEEECCCCEEECCEEEECCCCEEEEEEEE
VVANQEVKAGDVLATLDNGDYQNALDQAQAQIATEQLSLQRIDAQIEGANASLVQARAQK
EEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHH
VALEAAVRGAEITQKRQSELQAKSVGTAADLDNANIALDQAKANLAGGDANITAAQANIT
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECEEE
ILQAQRKEAEGSVRSLEISRDKAARDLSFTVLKAPYDGIVGNRSVQEGDLVSPGQRLMAL
EEEEHHHHCCCCEEEEEECHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHEEH
VPVRELYIDANFKETQIQHLVAGSKVNVHVDAYSDHPVVGTVESISPASGSVFSLLPPEN
CCEEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEHHCCCCCCCCEEEEECCCC
ATGNFTKVIQRVPVRIALPQDALDSGRLRAGLSVVVDVDTRTAPSK
CCCHHHHHHHHCCEEEECCCCCCCCCCEECCCEEEEEECCCCCCCC
>Mature Secondary Structure 
SSNQKSNVARIVSENAEAEEAKAEVVTVAEAPMAEALEKPSAPAQSAPASAPVAAPAAP
CCCCHHHHHHHHHCCCCHHHHHEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
KKRRSPVLPIVALVILAGGGWYGYEWWTNGRFMVSTDDAYIEGDIATISPKVTGYVARVN
HHCCCCCHHHHHHHHHCCCCEECEEEEECCEEEEECCCCEEECCEEEECCCCEEEEEEEE
VVANQEVKAGDVLATLDNGDYQNALDQAQAQIATEQLSLQRIDAQIEGANASLVQARAQK
EEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHH
VALEAAVRGAEITQKRQSELQAKSVGTAADLDNANIALDQAKANLAGGDANITAAQANIT
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECEEE
ILQAQRKEAEGSVRSLEISRDKAARDLSFTVLKAPYDGIVGNRSVQEGDLVSPGQRLMAL
EEEEHHHHCCCCEEEEEECHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHEEH
VPVRELYIDANFKETQIQHLVAGSKVNVHVDAYSDHPVVGTVESISPASGSVFSLLPPEN
CCEEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEHHCCCCCCCCEEEEECCCC
ATGNFTKVIQRVPVRIALPQDALDSGRLRAGLSVVVDVDTRTAPSK
CCCHHHHHHHHCCEEEECCCCCCCCCCEECCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]